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The map label for this gene is nac [H]

Identifier: 91788021

GI number: 91788021

Start: 2238587

End: 2239576

Strand: Direct

Name: nac [H]

Synonym: Bpro_2148

Alternate gene names: 91788021

Gene position: 2238587-2239576 (Clockwise)

Preceding gene: 91788014

Following gene: 91788022

Centisome position: 43.05

GC content: 63.94

Gene sequence:

>990_bases
TTGAATATTCAATATCACTGGGTACACTGTCGGGTACATCGGTCACCCGGATCCTCAGGATCCGTTCAGCAGGAGCCCAT
CATCGATTTACGCCGTCTTGAATATTTTGTGGCCGTTGCCGAGGCCGGCAGCTTCAGCAAGGCTGCAGCGGCGCTGGTGA
TGTCGCAGCCCGCGCTCAGCCAGCAGGTGGCCCTGCTCGAACAGGAAATCGGTCAACGCCTGCTCAACCGCACAGGGCGC
GGCGCCGAGCCCACCGACGCGGGGCTGGCGCTGCTGACGCACGCGCGGGCGATTTTTGAGCTGGCGGAACGGGCCCGTGC
TGACATGCAGGAGCGCAAGCTCATCCCCAGCGGCCGCATCACCATCGGCCTGCCGCCGCGCGTCGCCCACGCCATGACGG
CGGATTTGGTGGAGCGCTTTCGCGCCGAGTTCCCTGATGCCGCCATCAGCGTGGAAGAGGGGCTCAGCATTCGTCTGCGT
GAATGGCTGGTGGCCGGCCGCCTTGATGTGGCGGTGCTGTTTGACCCACCCGCGTCGCCGCTGCTGAATATTGAAACCAT
CGTGCGTGAGCCGTTGGTGCTGATGGCATCAACCCCCCTGCCACCACGCATCCGGCTGGCCGAGGTGGCGGCACTGTCAT
TGGTCCTGCCCAGCAAGCCCAATTCCTTGCGACAACTGCTTGAAAGCGAGGTCAAGCCCCGAGGTTACCTGCTCAAGGTG
GTGGCGGAGGTGGATTCGATAAAAACCGTGCTGTCGCTGGTTGCGCGCGGCGTGGCCGCAACCGTGCTGCCGGCCAGCGC
CATACGGGAATGGACGCATGACACACCGCCTTTCATGGCTGCCATCCATGCGCCGGTGATTCGCAACCGCCTGTGTGTTG
CGGTTCCCAAGGCGCGCCCGGCTACCCGGCTGAGCCGGTTTCTCGTGAGCCTGACGCGGGAGCTGTGCGAACAGTATTAC
GGCGATGCTGTGCACCAGCGAAAGCCCTGA

Upstream 100 bases:

>100_bases
GATTGAAGGTAAGGGTGGGTGTTGATGTCGTCATGGTGCTTGTCCGTATTCTGGCGGCGATGCCTCTGTTTTGGTTGGAT
GATTTTCAGATATCAGCTAT

Downstream 100 bases:

>100_bases
ACCCGTTGCTTCAGAACGCGCACTATCACGCACTGTCGCGGACTATCGCGCACTATTTTTGCTTCACCTTGTTTCCTTCT
GACTGTAGTAAATTAGATCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNIQYHWVHCRVHRSPGSSGSVQQEPIIDLRRLEYFVAVAEAGSFSKAAAALVMSQPALSQQVALLEQEIGQRLLNRTGR
GAEPTDAGLALLTHARAIFELAERARADMQERKLIPSGRITIGLPPRVAHAMTADLVERFRAEFPDAAISVEEGLSIRLR
EWLVAGRLDVAVLFDPPASPLLNIETIVREPLVLMASTPLPPRIRLAEVAALSLVLPSKPNSLRQLLESEVKPRGYLLKV
VAEVDSIKTVLSLVARGVAATVLPASAIREWTHDTPPFMAAIHAPVIRNRLCVAVPKARPATRLSRFLVSLTRELCEQYY
GDAVHQRKP

Sequences:

>Translated_329_residues
MNIQYHWVHCRVHRSPGSSGSVQQEPIIDLRRLEYFVAVAEAGSFSKAAAALVMSQPALSQQVALLEQEIGQRLLNRTGR
GAEPTDAGLALLTHARAIFELAERARADMQERKLIPSGRITIGLPPRVAHAMTADLVERFRAEFPDAAISVEEGLSIRLR
EWLVAGRLDVAVLFDPPASPLLNIETIVREPLVLMASTPLPPRIRLAEVAALSLVLPSKPNSLRQLLESEVKPRGYLLKV
VAEVDSIKTVLSLVARGVAATVLPASAIREWTHDTPPFMAAIHAPVIRNRLCVAVPKARPATRLSRFLVSLTRELCEQYY
GDAVHQRKP
>Mature_329_residues
MNIQYHWVHCRVHRSPGSSGSVQQEPIIDLRRLEYFVAVAEAGSFSKAAAALVMSQPALSQQVALLEQEIGQRLLNRTGR
GAEPTDAGLALLTHARAIFELAERARADMQERKLIPSGRITIGLPPRVAHAMTADLVERFRAEFPDAAISVEEGLSIRLR
EWLVAGRLDVAVLFDPPASPLLNIETIVREPLVLMASTPLPPRIRLAEVAALSLVLPSKPNSLRQLLESEVKPRGYLLKV
VAEVDSIKTVLSLVARGVAATVLPASAIREWTHDTPPFMAAIHAPVIRNRLCVAVPKARPATRLSRFLVSLTRELCEQYY
GDAVHQRKP

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=260, Percent_Identity=36.1538461538462, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI157672245, Length=241, Percent_Identity=33.6099585062241, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=34.7826086956522, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=31.0204081632653, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790399, Length=251, Percent_Identity=28.6852589641434, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787601, Length=242, Percent_Identity=24.7933884297521, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=22.9508196721311, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788748, Length=309, Percent_Identity=23.9482200647249, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789204, Length=241, Percent_Identity=26.9709543568465, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081904, Length=191, Percent_Identity=30.3664921465969, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87082132, Length=247, Percent_Identity=26.3157894736842, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=242, Percent_Identity=26.4462809917355, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788626, Length=164, Percent_Identity=34.7560975609756, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790208, Length=153, Percent_Identity=30.0653594771242, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787589, Length=147, Percent_Identity=35.3741496598639, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789506, Length=242, Percent_Identity=24.7933884297521, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367136, Length=247, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=30.1587301587302, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36180; Mature: 36180

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQYHWVHCRVHRSPGSSGSVQQEPIIDLRRLEYFVAVAEAGSFSKAAAALVMSQPALS
CCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
QQVALLEQEIGQRLLNRTGRGAEPTDAGLALLTHARAIFELAERARADMQERKLIPSGRI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TIGLPPRVAHAMTADLVERFRAEFPDAAISVEEGLSIRLREWLVAGRLDVAVLFDPPASP
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCHHHHHHHHHHCCCCEEEEECCCCCC
LLNIETIVREPLVLMASTPLPPRIRLAEVAALSLVLPSKPNSLRQLLESEVKPRGYLLKV
CHHHHHHHHCCHHEEECCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCHHHHHH
VAEVDSIKTVLSLVARGVAATVLPASAIREWTHDTPPFMAAIHAPVIRNRLCVAVPKARP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCH
ATRLSRFLVSLTRELCEQYYGDAVHQRKP
HHHHHHHHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure
MNIQYHWVHCRVHRSPGSSGSVQQEPIIDLRRLEYFVAVAEAGSFSKAAAALVMSQPALS
CCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
QQVALLEQEIGQRLLNRTGRGAEPTDAGLALLTHARAIFELAERARADMQERKLIPSGRI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TIGLPPRVAHAMTADLVERFRAEFPDAAISVEEGLSIRLREWLVAGRLDVAVLFDPPASP
EECCCHHHHHHHHHHHHHHHHHHCCCCCEEHHCCCHHHHHHHHHHCCCCEEEEECCCCCC
LLNIETIVREPLVLMASTPLPPRIRLAEVAALSLVLPSKPNSLRQLLESEVKPRGYLLKV
CHHHHHHHHCCHHEEECCCCCCCHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCHHHHHH
VAEVDSIKTVLSLVARGVAATVLPASAIREWTHDTPPFMAAIHAPVIRNRLCVAVPKARP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEECCCCH
ATRLSRFLVSLTRELCEQYYGDAVHQRKP
HHHHHHHHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]