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The map label for this gene is maiA [H]

Identifier: 91788009

GI number: 91788009

Start: 2227514

End: 2228152

Strand: Direct

Name: maiA [H]

Synonym: Bpro_2136

Alternate gene names: 91788009

Gene position: 2227514-2228152 (Clockwise)

Preceding gene: 91788007

Following gene: 91788010

Centisome position: 42.83

GC content: 60.25

Gene sequence:

>639_bases
ATGAAGCTTCACAACTATTTCCGTTCTTCCGCCTCCTTCCGCGTCCGGATTGCGCTTGAACTCAAGGCCCTGGACTATGA
GTACATTGCGGTGCACATTGCCCGGGGTGACCATAAAAAAGAGCCTTATGCAGCCCTTTCTGCCGACACTTTGGTGCCGC
TGCTGGAAGTGGACGGTGAAAAGCTGTCGCAGTCGATGGCCATCATCGAATACCTGGACGAAAAGCATCCGTCGCCGCCG
CTCCTGCCCGCCGATGCGGCAGGCCGCGCCAAGGTGCGTGCGCTGGCCCAGTCGATTGCCTGCGAGATTCACCCGCTCAA
CAACCTGCGCGTACTCAAGTACCTCGTGCGGGAACTCAAGGTGGAAGAAGAGGCCAAAAACACCTGGTACCGCCACTGGT
GCCGCGAAGGGCTGGAGGCCTTCGAGCGGCAGCTGGCCCAGTTGCCGGCCTCCACCTATTGCTATGGCAATACCCCCACC
CTGGCAGACTGCTGCCTGGTGCCGCAAATCTTCAACGCCAAACGCTTCAATGTGAACTTCGACGGCTTGCCCCGTACGAT
GGCGGCGTATGACGCCTGCATGGCGCTGCCGGCGTTCCAAAAGGCCCAGCCTTCGGCCTGCCCCGATAACGAAGCCTGA

Upstream 100 bases:

>100_bases
GGCGGAGAGTCGCGCAGGCGATTCGGCGGAACAGGTCGGCAACACTTTTGCAGCCTGTGCGGATGGCCGGCGCACGGCGT
TTCAGTTTATGCTTTCGGTC

Downstream 100 bases:

>100_bases
AAATGTGGCCCCCACGCTCCCCACTCCGTGTGGTTCGCTGCCCCCCGAGGGGGCTAATTTTCCTAGGGGCGGCCCGTCGG
AAAATTGCTCTGCAGACCAG

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKLHNYFRSSASFRVRIALELKALDYEYIAVHIARGDHKKEPYAALSADTLVPLLEVDGEKLSQSMAIIEYLDEKHPSPP
LLPADAAGRAKVRALAQSIACEIHPLNNLRVLKYLVRELKVEEEAKNTWYRHWCREGLEAFERQLAQLPASTYCYGNTPT
LADCCLVPQIFNAKRFNVNFDGLPRTMAAYDACMALPAFQKAQPSACPDNEA

Sequences:

>Translated_212_residues
MKLHNYFRSSASFRVRIALELKALDYEYIAVHIARGDHKKEPYAALSADTLVPLLEVDGEKLSQSMAIIEYLDEKHPSPP
LLPADAAGRAKVRALAQSIACEIHPLNNLRVLKYLVRELKVEEEAKNTWYRHWCREGLEAFERQLAQLPASTYCYGNTPT
LADCCLVPQIFNAKRFNVNFDGLPRTMAAYDACMALPAFQKAQPSACPDNEA
>Mature_212_residues
MKLHNYFRSSASFRVRIALELKALDYEYIAVHIARGDHKKEPYAALSADTLVPLLEVDGEKLSQSMAIIEYLDEKHPSPP
LLPADAAGRAKVRALAQSIACEIHPLNNLRVLKYLVRELKVEEEAKNTWYRHWCREGLEAFERQLAQLPASTYCYGNTPT
LADCCLVPQIFNAKRFNVNFDGLPRTMAAYDACMALPAFQKAQPSACPDNEA

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=42.8571428571429, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI22202622, Length=159, Percent_Identity=43.3962264150943, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI194440732, Length=210, Percent_Identity=32.3809523809524, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=38.4615384615385, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17556142, Length=197, Percent_Identity=38.0710659898477, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17510461, Length=208, Percent_Identity=34.6153846153846, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645375, Length=209, Percent_Identity=40.6698564593301, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355859, Length=209, Percent_Identity=40.6698564593301, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553325, Length=209, Percent_Identity=40.6698564593301, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21355857, Length=210, Percent_Identity=33.8095238095238, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23869; Mature: 23869

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHNYFRSSASFRVRIALELKALDYEYIAVHIARGDHKKEPYAALSADTLVPLLEVDGE
CCCHHHHHCCCCEEEEEEEEEEECCEEEEEEEEECCCCCCCCCHHHCHHHHHHHHHCCCH
KLSQSMAIIEYLDEKHPSPPLLPADAAGRAKVRALAQSIACEIHPLNNLRVLKYLVRELK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
VEEEAKNTWYRHWCREGLEAFERQLAQLPASTYCYGNTPTLADCCLVPQIFNAKRFNVNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCEEEECCC
DGLPRTMAAYDACMALPAFQKAQPSACPDNEA
CCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLHNYFRSSASFRVRIALELKALDYEYIAVHIARGDHKKEPYAALSADTLVPLLEVDGE
CCCHHHHHCCCCEEEEEEEEEEECCEEEEEEEEECCCCCCCCCHHHCHHHHHHHHHCCCH
KLSQSMAIIEYLDEKHPSPPLLPADAAGRAKVRALAQSIACEIHPLNNLRVLKYLVRELK
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHH
VEEEAKNTWYRHWCREGLEAFERQLAQLPASTYCYGNTPTLADCCLVPQIFNAKRFNVNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCEEEECCC
DGLPRTMAAYDACMALPAFQKAQPSACPDNEA
CCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]