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The map label for this gene is ltaA [H]

Identifier: 91787945

GI number: 91787945

Start: 2150588

End: 2151727

Strand: Direct

Name: ltaA [H]

Synonym: Bpro_2069

Alternate gene names: 91787945

Gene position: 2150588-2151727 (Clockwise)

Preceding gene: 91787944

Following gene: 91787946

Centisome position: 41.36

GC content: 70.26

Gene sequence:

>1140_bases
ATGAACAGCCATCCTGGCGACAACCACCCAGGCGGTCCTGCCCAGCCGCTGATGGACCTGCGCAGCGACACCGTGACCCG
TCCGACCGAGGCCATGTATGCCGCCATGGCCACAGCCGAGCTGGGAGACGACGGCCTCGACGGTGACCCGACCGCACGCC
TGCTGGAAGCCGAAACCGCCCAGTTGCTGGGCAAGGCCGCGGGCCTCTACGTACCCAGCGCCACCATGGCCAACCTGCTG
GCGGTGCTCATCCAGGCCGGGCGGCAGGAAACCGTGTTGATGGAGGCCACCAGCCACCTCTACCGCGCCGAACGTGGCGC
GGCGACCCTCACCGGCAGCTTCTTCGAAGGCATCACGGGCCAGGCTGGCGCGATGCACATGGACCTGCTGGCGACCGCCA
TCGCGCCGGGCCGCGGCAAGCTGCGGCCGGCGCTGGTCTGCATGGAAACCTCGCACAACGATGCCGGTGGCGCGGCGCTG
CCCTTGACCCACATGCAGGCGGTCCAGCAACTGGCGCAGCGGCACGGAGCCGCTGTTCACCTCGACGGCGCGCGGCTCTT
CAACGCCTCGGTGGCGCTGGGCCTGGCGCCCAAGGTTCTGGCCGATACGGCCTGTACGGTGTCGGTGTGCCTGTCCAAAG
GCCTGAGCGCGCCCATGGGGGCGGTCCTCGTGGGACCGGCGCCGGTCATTCAGCAGGCGCGCGGCCTGCGCAAGATGCTG
GGCGGCAGCCAGCGGCAGGTGGGCATCGCCGCCGCTGCGGGCCGTGTCGCCATTCACGCCATGGTCGCGCGCTTGGCCGA
CGACCACGAACGCGCTCGTCAACTGAGCCAGGGCCTGCACGGCGCGAAGGATTCGTCTGGCCTGCGCGTGAACTGGCCGC
AGACCAACATCGTGCAGGTCGACGTGTCCGCGACCGGCCTCGACGCCGAAGGGTGGACGTCGCGCCTTCGGGAGCACGGC
GTGCTGGTGCGGCCCTGGGGTCGTGCGCTGCTGCGCTGCGTGACACACCGCCACGTCGATGACATGCAAATTGCCGCCGT
GATCGAAGCCTTCCGCGCCGTGGCCGCGACGCTGCCGAGCACAGGTCACAGCCGTGACATTGGAAGCGACGTTGCCCGGC
CGGCCGCAGCCATCTCGTAG

Upstream 100 bases:

>100_bases
TGGGGCCCCATCGTCAAGGCCTCCGGCGCGAGTGCGGACTGATGCGCTCAGCCGTCCCCAATACCATCAACACGCCGCTC
ACTCTCTGCAAGGTATACCC

Downstream 100 bases:

>100_bases
CCACCGCATGGCGTGCCAGGTCGGCAAACACCGAGTCCGAGCGCGCCCGATTCAGCCGCCAGCCCGTCAGCCCAACCGCC
GCCGTCGAACGTCGACGTCA

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNSHPGDNHPGGPAQPLMDLRSDTVTRPTEAMYAAMATAELGDDGLDGDPTARLLEAETAQLLGKAAGLYVPSATMANLL
AVLIQAGRQETVLMEATSHLYRAERGAATLTGSFFEGITGQAGAMHMDLLATAIAPGRGKLRPALVCMETSHNDAGGAAL
PLTHMQAVQQLAQRHGAAVHLDGARLFNASVALGLAPKVLADTACTVSVCLSKGLSAPMGAVLVGPAPVIQQARGLRKML
GGSQRQVGIAAAAGRVAIHAMVARLADDHERARQLSQGLHGAKDSSGLRVNWPQTNIVQVDVSATGLDAEGWTSRLREHG
VLVRPWGRALLRCVTHRHVDDMQIAAVIEAFRAVAATLPSTGHSRDIGSDVARPAAAIS

Sequences:

>Translated_379_residues
MNSHPGDNHPGGPAQPLMDLRSDTVTRPTEAMYAAMATAELGDDGLDGDPTARLLEAETAQLLGKAAGLYVPSATMANLL
AVLIQAGRQETVLMEATSHLYRAERGAATLTGSFFEGITGQAGAMHMDLLATAIAPGRGKLRPALVCMETSHNDAGGAAL
PLTHMQAVQQLAQRHGAAVHLDGARLFNASVALGLAPKVLADTACTVSVCLSKGLSAPMGAVLVGPAPVIQQARGLRKML
GGSQRQVGIAAAAGRVAIHAMVARLADDHERARQLSQGLHGAKDSSGLRVNWPQTNIVQVDVSATGLDAEGWTSRLREHG
VLVRPWGRALLRCVTHRHVDDMQIAAVIEAFRAVAATLPSTGHSRDIGSDVARPAAAIS
>Mature_379_residues
MNSHPGDNHPGGPAQPLMDLRSDTVTRPTEAMYAAMATAELGDDGLDGDPTARLLEAETAQLLGKAAGLYVPSATMANLL
AVLIQAGRQETVLMEATSHLYRAERGAATLTGSFFEGITGQAGAMHMDLLATAIAPGRGKLRPALVCMETSHNDAGGAAL
PLTHMQAVQQLAQRHGAAVHLDGARLFNASVALGLAPKVLADTACTVSVCLSKGLSAPMGAVLVGPAPVIQQARGLRKML
GGSQRQVGIAAAAGRVAIHAMVARLADDHERARQLSQGLHGAKDSSGLRVNWPQTNIVQVDVSATGLDAEGWTSRLREHG
VLVRPWGRALLRCVTHRHVDDMQIAAVIEAFRAVAATLPSTGHSRDIGSDVARPAAAIS

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=343, Percent_Identity=37.3177842565598, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI193206524, Length=353, Percent_Identity=37.1104815864023, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI193206522, Length=353, Percent_Identity=37.1104815864023, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI193206520, Length=353, Percent_Identity=37.1104815864023, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6320789, Length=299, Percent_Identity=29.4314381270903, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21355439, Length=374, Percent_Identity=37.4331550802139, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 39410; Mature: 39410

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHPGDNHPGGPAQPLMDLRSDTVTRPTEAMYAAMATAELGDDGLDGDPTARLLEAETA
CCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QLLGKAAGLYVPSATMANLLAVLIQAGRQETVLMEATSHLYRAERGAATLTGSFFEGITG
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHCCCC
QAGAMHMDLLATAIAPGRGKLRPALVCMETSHNDAGGAALPLTHMQAVQQLAQRHGAAVH
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
LDGARLFNASVALGLAPKVLADTACTVSVCLSKGLSAPMGAVLVGPAPVIQQARGLRKML
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHH
GGSQRQVGIAAAAGRVAIHAMVARLADDHERARQLSQGLHGAKDSSGLRVNWPQTNIVQV
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEE
DVSATGLDAEGWTSRLREHGVLVRPWGRALLRCVTHRHVDDMQIAAVIEAFRAVAATLPS
EECCCCCCCHHHHHHHHHCCCEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
TGHSRDIGSDVARPAAAIS
CCCCCCCCHHHHCHHHCCC
>Mature Secondary Structure
MNSHPGDNHPGGPAQPLMDLRSDTVTRPTEAMYAAMATAELGDDGLDGDPTARLLEAETA
CCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QLLGKAAGLYVPSATMANLLAVLIQAGRQETVLMEATSHLYRAERGAATLTGSFFEGITG
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECHHHHCCCC
QAGAMHMDLLATAIAPGRGKLRPALVCMETSHNDAGGAALPLTHMQAVQQLAQRHGAAVH
CCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEE
LDGARLFNASVALGLAPKVLADTACTVSVCLSKGLSAPMGAVLVGPAPVIQQARGLRKML
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHH
GGSQRQVGIAAAAGRVAIHAMVARLADDHERARQLSQGLHGAKDSSGLRVNWPQTNIVQV
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEE
DVSATGLDAEGWTSRLREHGVLVRPWGRALLRCVTHRHVDDMQIAAVIEAFRAVAATLPS
EECCCCCCCHHHHHHHHHCCCEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
TGHSRDIGSDVARPAAAIS
CCCCCCCCHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} 0.12 {DL-threo-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]