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The map label for this gene is fumB [H]

Identifier: 91787922

GI number: 91787922

Start: 2125751

End: 2127385

Strand: Reverse

Name: fumB [H]

Synonym: Bpro_2046

Alternate gene names: 91787922

Gene position: 2127385-2125751 (Counterclockwise)

Preceding gene: 91787924

Following gene: 91787921

Centisome position: 40.91

GC content: 62.63

Gene sequence:

>1635_bases
GTGACCGCCCCGAAAAGACCGCCCCGAAAAGGCAACCCGGAAAAAGCCATTACCCCACCCAAGCACCTGACAGACCTGAC
CATGACGACCATCATCAAACAAGCCGACCTGATTGAATCCGTTGCCGCCGCCCTGCAGTACATCAGCTACTACCACCCCA
CCGACTACATCGCCCATCTGGCCCGTGCCTACGAGCGCGAGCAAAGCCCGGCGGCCAAGGATGCCATTGCGCAAATCCTG
ACCAACAGCAAGATGAGCGCCACGGGCCAGCGGCCAATCTGCCAGGACACCGGCATCGTCAACGTGTTTTTGAAGGTCGG
CATGGACGTGCGCTGGGAAGGCTTTACCGGCAGCATCGACGACGCCATCAACGAAGGCGTGCGCCGCGGCTACAACCACC
CCGACAACACGCTGCGCGCCTCCGTGGTGGCCGATCCGCAGTTCGACCGCAAGAACACCAAGGACAATACGCCCGCCGTG
ATCTTTACAGAGCTGGTGACCGGCAACACGGTTGAAGTCACGGTGGCAGCCAAAGGCGGCGGCAGTGAAAACAAGAGCAA
GATGGTGATGCTCAACCCCGGCGACTCGGTGGTCGACTGGGTGCTCAAAACCGTGCCCACCATGGGCGCCGGCTGGTGCC
CGCCCGGCATGCTGGGCATCGGCATTGGCGGCACCGCTGAAAAAGCCGTGCTGATGGCCAAGGAAAGCCTGATGGACGAC
CTGGACATGTACGAGCTGCAGGCCAAGTCGTCTGCGGGTGCCAAACTGACCAAAACCGAAGAGCTGCGCCTCGAACTGTT
CGAGAAAGTCAACGCGCTCGGCATTGGCGCGCAGGGCCTGGGTGGTCTCACCACCGTGCTCGACATCAAGATCAAGATGT
ACCCGACGCACGCGGCCAGCAAGCCTGTCGCCATGATCCCGAACTGCGCGGCCACGCGCCATGCACACTTTGTGCTCGAC
GGCAACGGCCCGGTCTACCTGGACCCGCCTTCGCTGGACCTCTGGCCCAATGTGAACTGGGAGCCCGACTACAACAAGAG
CAAAAAGGTCAACCTCAACACCCTGACCAAGGCAGAAGTCGCCAGCTGGAAGCCCGGCGACACCCTGCTGCTCAATGGCA
AGATGCTGACCGGTCGCGACGCCGCGCACAAACGCATTGCCGACATGCTGCTCAAGGGTGAAAAGCTGCCGGTCGACTTC
ACCAACCGCGTGATCTACTACGTCGGCCCGGTGGACCCGGTCAAGGGCGAGGCCGTTGGCCCGGCCGGCCCAACCACCGC
GACCCGCATGGACGGCTTCACCGAAATGATGCTGGCGCAAACCGGCTTGATCTCGATGGTGGGCAAGGCCGAGCGCGGCC
CCGTCGCGATTGAGGCCATCAAGAAGCACCAGAGCGCTTACCTGATGGCCGTGGGCGGCGCCGCCTACCTGGTATCCAAA
GCCATCAAGCACGCCGAAGTGGTGGGCTTTGCCGACCTGGGCATGGAAGCCATTTACGAGTTCGACGTGGTGGACATGCC
GGTGACGGTGGCGGTGGATGCCGGCGGCACCAGCGCGCACATCACCGGCCCGGCCGAGTGGCAGAAAAAAATTGCCACGG
GCGAGTTCAAGCAAATCCCGGTCACCAGCGCTTGA

Upstream 100 bases:

>100_bases
CTATGTGTGCCGGTACGGGTTTTCATGGTCATTTCCTCATGGCGCTGGGCCACCTGTTCACACCTGTCAAAATACGGGGT
TGCGGCTACCGTAGCACAGA

Downstream 100 bases:

>100_bases
GTCGCTTGCGGCGCTTGCACAAGGTTCGCGCTTGACCAGCCAGGCCATCGGGGTTTTCGACAGCGGCATCGGTGGCCTGA
GCATCCTCAAGGCGCTGCGG

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTAPKRPPRKGNPEKAITPPKHLTDLTMTTIIKQADLIESVAAALQYISYYHPTDYIAHLARAYEREQSPAAKDAIAQIL
TNSKMSATGQRPICQDTGIVNVFLKVGMDVRWEGFTGSIDDAINEGVRRGYNHPDNTLRASVVADPQFDRKNTKDNTPAV
IFTELVTGNTVEVTVAAKGGGSENKSKMVMLNPGDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDD
LDMYELQAKSSAGAKLTKTEELRLELFEKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLD
GNGPVYLDPPSLDLWPNVNWEPDYNKSKKVNLNTLTKAEVASWKPGDTLLLNGKMLTGRDAAHKRIADMLLKGEKLPVDF
TNRVIYYVGPVDPVKGEAVGPAGPTTATRMDGFTEMMLAQTGLISMVGKAERGPVAIEAIKKHQSAYLMAVGGAAYLVSK
AIKHAEVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIATGEFKQIPVTSA

Sequences:

>Translated_544_residues
MTAPKRPPRKGNPEKAITPPKHLTDLTMTTIIKQADLIESVAAALQYISYYHPTDYIAHLARAYEREQSPAAKDAIAQIL
TNSKMSATGQRPICQDTGIVNVFLKVGMDVRWEGFTGSIDDAINEGVRRGYNHPDNTLRASVVADPQFDRKNTKDNTPAV
IFTELVTGNTVEVTVAAKGGGSENKSKMVMLNPGDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDD
LDMYELQAKSSAGAKLTKTEELRLELFEKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLD
GNGPVYLDPPSLDLWPNVNWEPDYNKSKKVNLNTLTKAEVASWKPGDTLLLNGKMLTGRDAAHKRIADMLLKGEKLPVDF
TNRVIYYVGPVDPVKGEAVGPAGPTTATRMDGFTEMMLAQTGLISMVGKAERGPVAIEAIKKHQSAYLMAVGGAAYLVSK
AIKHAEVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIATGEFKQIPVTSA
>Mature_543_residues
TAPKRPPRKGNPEKAITPPKHLTDLTMTTIIKQADLIESVAAALQYISYYHPTDYIAHLARAYEREQSPAAKDAIAQILT
NSKMSATGQRPICQDTGIVNVFLKVGMDVRWEGFTGSIDDAINEGVRRGYNHPDNTLRASVVADPQFDRKNTKDNTPAVI
FTELVTGNTVEVTVAAKGGGSENKSKMVMLNPGDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDDL
DMYELQAKSSAGAKLTKTEELRLELFEKVNALGIGAQGLGGLTTVLDIKIKMYPTHAASKPVAMIPNCAATRHAHFVLDG
NGPVYLDPPSLDLWPNVNWEPDYNKSKKVNLNTLTKAEVASWKPGDTLLLNGKMLTGRDAAHKRIADMLLKGEKLPVDFT
NRVIYYVGPVDPVKGEAVGPAGPTTATRMDGFTEMMLAQTGLISMVGKAERGPVAIEAIKKHQSAYLMAVGGAAYLVSKA
IKHAEVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIATGEFKQIPVTSA

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=470, Percent_Identity=31.7021276595745, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787897, Length=470, Percent_Identity=33.4042553191489, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789442, Length=294, Percent_Identity=26.8707482993197, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789443, Length=172, Percent_Identity=29.6511627906977, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 58397; Mature: 58266

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPKRPPRKGNPEKAITPPKHLTDLTMTTIIKQADLIESVAAALQYISYYHPTDYIAHL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ARAYEREQSPAAKDAIAQILTNSKMSATGQRPICQDTGIVNVFLKVGMDVRWEGFTGSID
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEHHHCCCCEEECCCCCCHH
DAINEGVRRGYNHPDNTLRASVVADPQFDRKNTKDNTPAVIFTELVTGNTVEVTVAAKGG
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCC
GSENKSKMVMLNPGDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDD
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHH
LDMYELQAKSSAGAKLTKTEELRLELFEKVNALGIGAQGLGGLTTVLDIKIKMYPTHAAS
HHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHEECCCCCCCCEEEEEEEEEEEEECCCCC
KPVAMIPNCAATRHAHFVLDGNGPVYLDPPSLDLWPNVNWEPDYNKSKKVNLNTLTKAEV
CCEEECCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHH
ASWKPGDTLLLNGKMLTGRDAAHKRIADMLLKGEKLPVDFTNRVIYYVGPVDPVKGEAVG
CCCCCCCEEEECCEEECCCHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCCC
PAGPTTATRMDGFTEMMLAQTGLISMVGKAERGPVAIEAIKKHQSAYLMAVGGAAYLVSK
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHH
AIKHAEVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIATGEFKQIP
HHHHHHHEEHHHHCHHHHEEEEEECCCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCC
VTSA
CCCC
>Mature Secondary Structure 
TAPKRPPRKGNPEKAITPPKHLTDLTMTTIIKQADLIESVAAALQYISYYHPTDYIAHL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ARAYEREQSPAAKDAIAQILTNSKMSATGQRPICQDTGIVNVFLKVGMDVRWEGFTGSID
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHEEHHHCCCCEEECCCCCCHH
DAINEGVRRGYNHPDNTLRASVVADPQFDRKNTKDNTPAVIFTELVTGNTVEVTVAAKGG
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCC
GSENKSKMVMLNPGDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDD
CCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHH
LDMYELQAKSSAGAKLTKTEELRLELFEKVNALGIGAQGLGGLTTVLDIKIKMYPTHAAS
HHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHEECCCCCCCCEEEEEEEEEEEEECCCCC
KPVAMIPNCAATRHAHFVLDGNGPVYLDPPSLDLWPNVNWEPDYNKSKKVNLNTLTKAEV
CCEEECCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHH
ASWKPGDTLLLNGKMLTGRDAAHKRIADMLLKGEKLPVDFTNRVIYYVGPVDPVKGEAVG
CCCCCCCEEEECCEEECCCHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCCC
PAGPTTATRMDGFTEMMLAQTGLISMVGKAERGPVAIEAIKKHQSAYLMAVGGAAYLVSK
CCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEEECHHHHHHHH
AIKHAEVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIATGEFKQIP
HHHHHHHEEHHHHCHHHHEEEEEECCCEEEEEECCCCEEEECCHHHHHHHHCCCCCCCCC
VTSA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]