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The map label for this gene is nac [H]

Identifier: 91787657

GI number: 91787657

Start: 1833762

End: 1834676

Strand: Direct

Name: nac [H]

Synonym: Bpro_1775

Alternate gene names: 91787657

Gene position: 1833762-1834676 (Clockwise)

Preceding gene: 91787653

Following gene: 91787658

Centisome position: 35.26

GC content: 60.22

Gene sequence:

>915_bases
ATGAATCTCCGGTTTGTTGAAGCTTTCTACTGGGTGGCATCACTGAAAAGCATCTCGCGGGCGGCTGAAAAGCTGTACCT
GACGCAGTCCGCCATGTCCAGCCGCATCGCCACGCTGGAAGAAGAACTGGGCATCCTGCTACTGGACCGGCGCGACAAGC
AGTTCAAGCTCACGGTGGCCGGTGCCCGTTTTCTGGTTTATGCGCAAAAGCTGCTGGAACTGCAGCGCGAAGTCAAAGCC
GAGATGGGGTCGGGGCAGGCCATGGCGATGTCCATGCGCATTGGCGCGATCGAGTCGGTGCTGCACTCCTGGCTGATTCC
CTGGCTGGAAAAGCTGCGTGCCGACTACCCGGGGCTGGAACTGGAACTGACGGTGGAAACCACGCCGATCCTGATCGACC
AGATCCAGCGCGGCACGCTGGACCTGGTCTTTGCGGTGTTGCCCGCGTCGGCCGAGGGCGTGCGAAGCCAGGCCTTGCCA
TCCATGGACATGGCGTTTGTCGGGCACCCGGACCTGCACAAAAAAAGAATTTACAAACTCGATGAGCTGGCCGGGATGGA
GTTGTTGACCTTCCAGAAAGGCTCCCAGCCGCATGTCACGCTGCTTGATTTGTTCAGGCAGCGCCGCATGGAACCCAAAA
AGGTGCACGCCATTTCCTCAATTTCGGCGATGGTTCAGCTGGTGCAGGGCGGCTTCGGGGTGGCCTTGTTGCCGCGCGCC
GCGGTAGAGCGGCTCACCGGCTTTGCGCGCCTGAAGCTGCTGACCTGCGACGCCAAGTTGCAGTCCTTGCCGATCAACGC
CAGTTACCGCACCGACCCCAGTTCCAGGACTGTGGAGACCGTTGTGCAGTCGGCCATTGCTTTCGCCGGAGGCCGCAACA
ACGCACCAAAAAGGCGACTAAAGGTAAACCCTTGA

Upstream 100 bases:

>100_bases
ACAATAGGGTGATAAGTATGAAGAGGATAGACGCAAATCCCGCGCCAGCTTCCCCGGACATACCCCGAACACCTTAACGC
CCGCTACAGAAGGCCCCCTC

Downstream 100 bases:

>100_bases
TATCGAAAAAATCGATGGTGTAAGCAAAAAATTTTGAATTTGCTGAAGGCTGAAGACCCCCACACAATTCGCTCATCGTC
ATCAAGGTGACACATGCAAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNLRFVEAFYWVASLKSISRAAEKLYLTQSAMSSRIATLEEELGILLLDRRDKQFKLTVAGARFLVYAQKLLELQREVKA
EMGSGQAMAMSMRIGAIESVLHSWLIPWLEKLRADYPGLELELTVETTPILIDQIQRGTLDLVFAVLPASAEGVRSQALP
SMDMAFVGHPDLHKKRIYKLDELAGMELLTFQKGSQPHVTLLDLFRQRRMEPKKVHAISSISAMVQLVQGGFGVALLPRA
AVERLTGFARLKLLTCDAKLQSLPINASYRTDPSSRTVETVVQSAIAFAGGRNNAPKRRLKVNP

Sequences:

>Translated_304_residues
MNLRFVEAFYWVASLKSISRAAEKLYLTQSAMSSRIATLEEELGILLLDRRDKQFKLTVAGARFLVYAQKLLELQREVKA
EMGSGQAMAMSMRIGAIESVLHSWLIPWLEKLRADYPGLELELTVETTPILIDQIQRGTLDLVFAVLPASAEGVRSQALP
SMDMAFVGHPDLHKKRIYKLDELAGMELLTFQKGSQPHVTLLDLFRQRRMEPKKVHAISSISAMVQLVQGGFGVALLPRA
AVERLTGFARLKLLTCDAKLQSLPINASYRTDPSSRTVETVVQSAIAFAGGRNNAPKRRLKVNP
>Mature_304_residues
MNLRFVEAFYWVASLKSISRAAEKLYLTQSAMSSRIATLEEELGILLLDRRDKQFKLTVAGARFLVYAQKLLELQREVKA
EMGSGQAMAMSMRIGAIESVLHSWLIPWLEKLRADYPGLELELTVETTPILIDQIQRGTLDLVFAVLPASAEGVRSQALP
SMDMAFVGHPDLHKKRIYKLDELAGMELLTFQKGSQPHVTLLDLFRQRRMEPKKVHAISSISAMVQLVQGGFGVALLPRA
AVERLTGFARLKLLTCDAKLQSLPINASYRTDPSSRTVETVVQSAIAFAGGRNNAPKRRLKVNP

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=29.3172690763052, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=22.9681978798587, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790399, Length=250, Percent_Identity=28.8, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994962, Length=169, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=249, Percent_Identity=28.9156626506024, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=26, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=263, Percent_Identity=25.8555133079848, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=279, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.4295532646048, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=241, Percent_Identity=23.6514522821577, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=239, Percent_Identity=28.8702928870293, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=254, Percent_Identity=24.4094488188976, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI145693193, Length=284, Percent_Identity=25.7042253521127, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33873; Mature: 33873

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRFVEAFYWVASLKSISRAAEKLYLTQSAMSSRIATLEEELGILLLDRRDKQFKLTVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEC
GARFLVYAQKLLELQREVKAEMGSGQAMAMSMRIGAIESVLHSWLIPWLEKLRADYPGLE
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LELTVETTPILIDQIQRGTLDLVFAVLPASAEGVRSQALPSMDMAFVGHPDLHKKRIYKL
EEEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCHHHHHHHHH
DELAGMELLTFQKGSQPHVTLLDLFRQRRMEPKKVHAISSISAMVQLVQGGFGVALLPRA
HHHCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
AVERLTGFARLKLLTCDAKLQSLPINASYRTDPSSRTVETVVQSAIAFAGGRNNAPKRRL
HHHHHHHHHHEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
KVNP
CCCC
>Mature Secondary Structure
MNLRFVEAFYWVASLKSISRAAEKLYLTQSAMSSRIATLEEELGILLLDRRDKQFKLTVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEC
GARFLVYAQKLLELQREVKAEMGSGQAMAMSMRIGAIESVLHSWLIPWLEKLRADYPGLE
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LELTVETTPILIDQIQRGTLDLVFAVLPASAEGVRSQALPSMDMAFVGHPDLHKKRIYKL
EEEEECCCHHHHHHHCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEECCCCCHHHHHHHHH
DELAGMELLTFQKGSQPHVTLLDLFRQRRMEPKKVHAISSISAMVQLVQGGFGVALLPRA
HHHCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHH
AVERLTGFARLKLLTCDAKLQSLPINASYRTDPSSRTVETVVQSAIAFAGGRNNAPKRRL
HHHHHHHHHHEEEEEECCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
KVNP
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]