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The map label for this gene is guaC

Identifier: 91787600

GI number: 91787600

Start: 1771115

End: 1772092

Strand: Reverse

Name: guaC

Synonym: Bpro_1718

Alternate gene names: 91787600

Gene position: 1772092-1771115 (Counterclockwise)

Preceding gene: 91787601

Following gene: 91787599

Centisome position: 34.08

GC content: 59.51

Gene sequence:

>978_bases
ATGGAAATCTTCGATTACGAAAACGTGCTGCTGCTGCCGCGCAAATGCCGGGTGGAAAGCCGCTCGGAATGCAATGCCGG
TGTGGCGCTGGGCGGGCGCACCTTCCGTATCCCGGTGGTGCCGGCCAACATGAAAACGGTGGTCGACGAAAACATCTGTG
CCTGGATGGCCAAAAACGGCTACTTCTACGTGATGCACCGCTTTGACCTGGACAACCTGCAGTTCGTCAAAAACATGAAG
GCCAAAGGCGTTTACGCGTCGATCTCGCTGGGCGTCAAAAAACCAGACTACGACACGGTGGACCAGTTTGTGGCCGAGGG
TCTCGCCCCCGAATACATCACGATCGACATTGCCCATGGCCATGCCGACACCGTGCAGCGCATGATCGCCTACCTGAAAC
AGAAACTGCCTGCATCGTTCGTGATTGCCGGCAACGTGGCCACACCCGAAGCGGTGATTGACCTGGAAAACTGGGGCGCC
GATGCGACCAAGGTGGGCATCGGCCCCGGCAAGGTCTGCATCACGAAAATGAAGACGGGCTTTGGCACCGGCGGCTGGCA
GCTCTCGGCGCTCAAATGGTGCGCCCGGGTGGCGACCAAGCCCATCATTGCCGACGGCGGCATCCGCGAACACGGTGACA
TTGCCAAGTCCATCCGTTTTGGCGCGACCATGGTGATGATCGGCTCCCTGCTCGCCGGCCTGGAAGAAAGCCCCGGCAAG
ACCGTGGAGGTCGATGGCAAGCTCTTCAAGGAGTATTACGGCAGCGCCTCGGACTTCAACAAGGGCGAGTACAAGCACGT
TGAAGGCAAACGCATCCTGGAACCCATCAAGGGCACGCTGGCGGACACCCTGCGCGAGATGGAAGAAGACATCCAGAGCT
CCATCAGCTATGCCGGGGGCAAAAAACTGATGGACATCCGCAAGGCCAACTACGTGATCCTGGGCGGCGACAACGCAGGC
GAGCACCTGCTGATGTGA

Upstream 100 bases:

>100_bases
TTTGCGCCTTGCGGCTCATCCCGCAAAAGCCCCGCCGCGCGCGGCGCGAGTTAGACAGAGCATCCCTGGTTATCAACAGT
CTTCAGGAGCTTGGGAAACT

Downstream 100 bases:

>100_bases
GGCTTGGCCGATCCAGCCTGCCGCAGGAGTACAGTCATCGCCAAACCATGAAAGCGAGGATCCGTGCCCACTGCAGCCAT
TGAAATACGCCGAAAATATT

Product: guanosine 5'-monophosphate oxidoreductase

Products: NA

Alternate protein names: Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MEIFDYENVLLLPRKCRVESRSECNAGVALGGRTFRIPVVPANMKTVVDENICAWMAKNGYFYVMHRFDLDNLQFVKNMK
AKGVYASISLGVKKPDYDTVDQFVAEGLAPEYITIDIAHGHADTVQRMIAYLKQKLPASFVIAGNVATPEAVIDLENWGA
DATKVGIGPGKVCITKMKTGFGTGGWQLSALKWCARVATKPIIADGGIREHGDIAKSIRFGATMVMIGSLLAGLEESPGK
TVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPIKGTLADTLREMEEDIQSSISYAGGKKLMDIRKANYVILGGDNAG
EHLLM

Sequences:

>Translated_325_residues
MEIFDYENVLLLPRKCRVESRSECNAGVALGGRTFRIPVVPANMKTVVDENICAWMAKNGYFYVMHRFDLDNLQFVKNMK
AKGVYASISLGVKKPDYDTVDQFVAEGLAPEYITIDIAHGHADTVQRMIAYLKQKLPASFVIAGNVATPEAVIDLENWGA
DATKVGIGPGKVCITKMKTGFGTGGWQLSALKWCARVATKPIIADGGIREHGDIAKSIRFGATMVMIGSLLAGLEESPGK
TVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPIKGTLADTLREMEEDIQSSISYAGGKKLMDIRKANYVILGGDNAG
EHLLM
>Mature_325_residues
MEIFDYENVLLLPRKCRVESRSECNAGVALGGRTFRIPVVPANMKTVVDENICAWMAKNGYFYVMHRFDLDNLQFVKNMK
AKGVYASISLGVKKPDYDTVDQFVAEGLAPEYITIDIAHGHADTVQRMIAYLKQKLPASFVIAGNVATPEAVIDLENWGA
DATKVGIGPGKVCITKMKTGFGTGGWQLSALKWCARVATKPIIADGGIREHGDIAKSIRFGATMVMIGSLLAGLEESPGK
TVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPIKGTLADTLREMEEDIQSSISYAGGKKLMDIRKANYVILGGDNAG
EHLLM

Specific function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI50541954, Length=327, Percent_Identity=33.0275229357798, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI50541952, Length=327, Percent_Identity=33.0275229357798, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI50541948, Length=327, Percent_Identity=33.0275229357798, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI50541956, Length=327, Percent_Identity=33.0275229357798, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI156104880, Length=327, Percent_Identity=32.4159021406728, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI66933016, Length=179, Percent_Identity=35.195530726257, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI217035148, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI217035146, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI217035150, Length=171, Percent_Identity=35.672514619883, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI217035152, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI156616279, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI34328928, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI34328930, Length=178, Percent_Identity=34.8314606741573, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1786293, Length=330, Percent_Identity=31.5151515151515, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1788855, Length=165, Percent_Identity=42.4242424242424, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17560440, Length=323, Percent_Identity=32.1981424148607, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71994385, Length=145, Percent_Identity=40.6896551724138, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994389, Length=145, Percent_Identity=40.6896551724138, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323585, Length=183, Percent_Identity=32.7868852459016, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322012, Length=183, Percent_Identity=32.7868852459016, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323464, Length=183, Percent_Identity=32.7868852459016, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571163, Length=173, Percent_Identity=37.5722543352601, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24641071, Length=180, Percent_Identity=36.6666666666667, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641073, Length=180, Percent_Identity=36.6666666666667, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GUAC_POLSJ (Q12CT8)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548552.1
- ProteinModelPortal:   Q12CT8
- SMR:   Q12CT8
- STRING:   Q12CT8
- GeneID:   4015620
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1718
- NMPDR:   fig|296591.1.peg.4021
- eggNOG:   COG0516
- HOGENOM:   HBG298985
- OMA:   PDYITID
- PhylomeDB:   Q12CT8
- ProtClustDB:   PRK05458
- BioCyc:   PSP296591:BPRO_1718-MONOMER
- HAMAP:   MF_01511
- InterPro:   IPR013785
- InterPro:   IPR005994
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF036500
- TIGRFAMs:   TIGR01306

Pfam domain/function: PF00478 IMPDH

EC number: =1.7.1.7

Molecular weight: Translated: 35567; Mature: 35567

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFDYENVLLLPRKCRVESRSECNAGVALGGRTFRIPVVPANMKTVVDENICAWMAKNG
CCCCCCCCEEEECHHCCCCCCCCCCCCEEECCEEEEEEEECCCHHHHHHHHHHEEEECCC
YFYVMHRFDLDNLQFVKNMKAKGVYASISLGVKKPDYDTVDQFVAEGLAPEYITIDIAHG
EEEEEEECCCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECC
HADTVQRMIAYLKQKLPASFVIAGNVATPEAVIDLENWGADATKVGIGPGKVCITKMKTG
CHHHHHHHHHHHHHHCCCEEEEECCCCCCHHEEEEHHCCCCCEEECCCCCCEEEEEECCC
FGTGGWQLSALKWCARVATKPIIADGGIREHGDIAKSIRFGATMVMIGSLLAGLEESPGK
CCCCCEEHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPIKGTLADTLREMEEDIQSSISYAGG
EEEECHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KKLMDIRKANYVILGGDNAGEHLLM
HHHEEEHHCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MEIFDYENVLLLPRKCRVESRSECNAGVALGGRTFRIPVVPANMKTVVDENICAWMAKNG
CCCCCCCCEEEECHHCCCCCCCCCCCCEEECCEEEEEEEECCCHHHHHHHHHHEEEECCC
YFYVMHRFDLDNLQFVKNMKAKGVYASISLGVKKPDYDTVDQFVAEGLAPEYITIDIAHG
EEEEEEECCCCHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECC
HADTVQRMIAYLKQKLPASFVIAGNVATPEAVIDLENWGADATKVGIGPGKVCITKMKTG
CHHHHHHHHHHHHHHCCCEEEEECCCCCCHHEEEEHHCCCCCEEECCCCCCEEEEEECCC
FGTGGWQLSALKWCARVATKPIIADGGIREHGDIAKSIRFGATMVMIGSLLAGLEESPGK
CCCCCEEHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPIKGTLADTLREMEEDIQSSISYAGG
EEEECHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KKLMDIRKANYVILGGDNAGEHLLM
HHHEEEHHCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA