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The map label for this gene is pcm [H]

Identifier: 91787548

GI number: 91787548

Start: 1717964

End: 1718665

Strand: Reverse

Name: pcm [H]

Synonym: Bpro_1666

Alternate gene names: 91787548

Gene position: 1718665-1717964 (Counterclockwise)

Preceding gene: 91787549

Following gene: 91787547

Centisome position: 33.05

GC content: 63.53

Gene sequence:

>702_bases
ATGAACTACGAACAAGCCCGATTCAACATGATTGAGCAGCAAATCCGCCCCTGGGAAGTGCTGGACAGCCAGGTCCTTTC
ACTGCTCGCCGTGGTCAAGCGCGAAGACTTCGTACCGGCCGCCTACCGGGCGCTGGCGTTTATGGACATGGAAATTCCGC
TGACCGCCGAAGCGGCGCACCACAAAAACCCCGGCCAGTGCATGCTGGCGCCCAAGGTCGAAGCCCGCATCCTGCAGGAC
GTGGCCGTGCAGAAACATGAGAAGGTGCTCGAGATCGGCGCCGGTTCCGGTTACATGGCGGCGCTGCTGGCGCACCGTGC
CCAGCAGGTGATCACGCTGGAGATCGACCCCACGCTGGCCCAGATGGCACGGAGCAACCTGCAAAAAGCCGGCCTCTACA
ACGCCGAAGTGCGTACCGGCGACGGCGCTGCCAATCTTGCGCAGGCCGTGTCCAGCAACGACCCGCTGCACGGACCGTTT
GACGTGATTGTGCTCAGCGGTTCCGTCGCCGAGGTGCCCGCTTCGCTGCTGTCGCTGCTCAAGGTCGGCGGACGCCTGAG
CGCCATCGTGGGTTTTGAGCCCGTGATGCGCGCCACCCTGGTCACGCGTGTCGCCGAAGAAGCCTGGCGCACCACCCATA
CCTGGGACACGGTGGCGCCGCGGTTGCTGAACTTTCCCGAGCCTTCGAAATTCAACTTCTGA

Upstream 100 bases:

>100_bases
CTTCCGCCGGCCCGGCTTCCGTCTCCAGCCCCGGCTAAAATCAAGGGTTTATCTCGTGCACTCCCCTTCCTTTCTTACCC
CGCCAACCCACAGGTCCCCC

Downstream 100 bases:

>100_bases
CAAGCGGCTTGAACTTTTTTTGCCACCACCTGCCAACCAGCAACCAAGGGCACCGCAATGATTTCCCAACTGAATCCTTC
TGACTTCGCCGCCTGGCGTG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MNYEQARFNMIEQQIRPWEVLDSQVLSLLAVVKREDFVPAAYRALAFMDMEIPLTAEAAHHKNPGQCMLAPKVEARILQD
VAVQKHEKVLEIGAGSGYMAALLAHRAQQVITLEIDPTLAQMARSNLQKAGLYNAEVRTGDGAANLAQAVSSNDPLHGPF
DVIVLSGSVAEVPASLLSLLKVGGRLSAIVGFEPVMRATLVTRVAEEAWRTTHTWDTVAPRLLNFPEPSKFNF

Sequences:

>Translated_233_residues
MNYEQARFNMIEQQIRPWEVLDSQVLSLLAVVKREDFVPAAYRALAFMDMEIPLTAEAAHHKNPGQCMLAPKVEARILQD
VAVQKHEKVLEIGAGSGYMAALLAHRAQQVITLEIDPTLAQMARSNLQKAGLYNAEVRTGDGAANLAQAVSSNDPLHGPF
DVIVLSGSVAEVPASLLSLLKVGGRLSAIVGFEPVMRATLVTRVAEEAWRTTHTWDTVAPRLLNFPEPSKFNF
>Mature_233_residues
MNYEQARFNMIEQQIRPWEVLDSQVLSLLAVVKREDFVPAAYRALAFMDMEIPLTAEAAHHKNPGQCMLAPKVEARILQD
VAVQKHEKVLEIGAGSGYMAALLAHRAQQVITLEIDPTLAQMARSNLQKAGLYNAEVRTGDGAANLAQAVSSNDPLHGPF
DVIVLSGSVAEVPASLLSLLKVGGRLSAIVGFEPVMRATLVTRVAEEAWRTTHTWDTVAPRLLNFPEPSKFNF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=187, Percent_Identity=34.2245989304813, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 25524; Mature: 25524

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYEQARFNMIEQQIRPWEVLDSQVLSLLAVVKREDFVPAAYRALAFMDMEIPLTAEAAH
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHC
HKNPGQCMLAPKVEARILQDVAVQKHEKVLEIGAGSGYMAALLAHRAQQVITLEIDPTLA
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECHHHH
QMARSNLQKAGLYNAEVRTGDGAANLAQAVSSNDPLHGPFDVIVLSGSVAEVPASLLSLL
HHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHH
KVGGRLSAIVGFEPVMRATLVTRVAEEAWRTTHTWDTVAPRLLNFPEPSKFNF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNYEQARFNMIEQQIRPWEVLDSQVLSLLAVVKREDFVPAAYRALAFMDMEIPLTAEAAH
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHC
HKNPGQCMLAPKVEARILQDVAVQKHEKVLEIGAGSGYMAALLAHRAQQVITLEIDPTLA
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECHHHH
QMARSNLQKAGLYNAEVRTGDGAANLAQAVSSNDPLHGPFDVIVLSGSVAEVPASLLSLL
HHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHH
KVGGRLSAIVGFEPVMRATLVTRVAEEAWRTTHTWDTVAPRLLNFPEPSKFNF
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA