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The map label for this gene is argG

Identifier: 91787483

GI number: 91787483

Start: 1650757

End: 1652097

Strand: Reverse

Name: argG

Synonym: Bpro_1599

Alternate gene names: 91787483

Gene position: 1652097-1650757 (Counterclockwise)

Preceding gene: 91787484

Following gene: 91787480

Centisome position: 31.77

GC content: 64.88

Gene sequence:

>1341_bases
ATGGCCACCATCCTCCAGAACCTGCCCGCCGGCCAGAAAGTCGGCATCGCCTTTTCCGGCGGCCTTGATACCAGCGCCGC
GCTGCACTGGATGAAACTCAAGGGCGCCCTTCCCTACGCCTACACGGCCAACCTGGGCCAGCCCGACGAGCCCGACTACG
ACGAGATCCCACGCAAGGCATTGCAGTACGGCGCCGAGAAGGCCCGCCTGATCGACTGCCGCAGCCAGCTCGCCGCCGAA
GGCATTGCCGCCCTGCAAAGCGGCGCCTTCCACATCACGACCGCAGGCGTCACCTACTTCAACACCACGCCGCTGGGCCG
CGCCGTGACCGGCACAATGCTGGTGTCCGCCATGAAGGACGACGACGTCAACATCTGGGGCGACGGCAGCACCTTCAAGG
GCAACGACATCGAGCGCTTTTACCGCTACGGCCTGCTGACCAACCCCAACCTCCGGATTTACAAGCCCTGGCTGGACCAG
CTCTTCATTGACGAGCTGGGCGGGCGCGCCGAAATGTCGGCCTTCATGACGCAGGCCGGTTTCGGCTACAAGATGTCGGC
CGAAAAGGCCTACTCCACCGACTCCAACATGCTGGGCGCCACCCACGAAGCCAAGGACCTGGAGCACCTGAACAGCGGCA
TGAAGATCGTGAACCCGATCATGGGCGTGGCCTTCTGGAAAGACGAGGTCGAGGTCAAGCGCGAGGAAGTCACCGTGCGC
TTCGAGGAAGGCCAGCCCGTCGCGCTCAACGGCAAGACGTTTTCCAACACTGTTGAGTTGATCCTCGAAGCCAACCGCAT
TGGCGGGCGCCACGGCCTGGGCATGAGCGACCAGATCGAAAACCGCATCATCGAGGCCAAGAGCCGCGGCATCTACGAGG
CACCCGGCCTGGCGCTGCTGTTCATCGCTTACGAGCGCCTGGTGACCGGCATCCACAACGAAGACACGATCGAGCAGTAC
CGCGACAACGGCCGGCGCCTGGGCCGCCTGCTGTACCAGGGCCGCTGGTTCGACCCGCAGGCCATCATGCTGCGCGAGAC
GGCGCAGCGCTGGGTAGCGCGTGCGGTCACCGGCGAGGTCACGGTGGAACTGCGCCGCGGCAACGACTACTCGATCCTGA
ACACGGTCTCGCCGAACCTGACCTACAAGCCCGAGCGGCTGAGCATGGAAAAGGTGGAGGACGCGCCGTTCTCCCCGCTG
GACCGCATCGGCCAGCTGACCATGCGCAACCTCGACATCGTCGACACGCGCGACAAGCTCGCGATCTACACGCAGGCCGG
CCTGCTGTCAGCCGGCCAGGGCGCGTCGCCGCCGCAACTGAAGAACGACGAACGCAAGTGA

Upstream 100 bases:

>100_bases
GGGCAACGCGAAGTTTGACATCCGCGTGGCAGAAGGCTTCGAGGCCTACCACTACATCTGCCACTTCGGTTAATCGCCCC
TCCCCTCAGGAACCCATTTC

Downstream 100 bases:

>100_bases
GCCGTTGCGGAGGCCGGGGCCGCGATCAGTCGCCTCCGCCGCCGCACCCTCCGCCGCAGCCCGAGCCGCCATCGCCTGAC
GAACCGCCGTCGCCGCCGCT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MATILQNLPAGQKVGIAFSGGLDTSAALHWMKLKGALPYAYTANLGQPDEPDYDEIPRKALQYGAEKARLIDCRSQLAAE
GIAALQSGAFHITTAGVTYFNTTPLGRAVTGTMLVSAMKDDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLRIYKPWLDQ
LFIDELGGRAEMSAFMTQAGFGYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGMKIVNPIMGVAFWKDEVEVKREEVTVR
FEEGQPVALNGKTFSNTVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQY
RDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAVTGEVTVELRRGNDYSILNTVSPNLTYKPERLSMEKVEDAPFSPL
DRIGQLTMRNLDIVDTRDKLAIYTQAGLLSAGQGASPPQLKNDERK

Sequences:

>Translated_446_residues
MATILQNLPAGQKVGIAFSGGLDTSAALHWMKLKGALPYAYTANLGQPDEPDYDEIPRKALQYGAEKARLIDCRSQLAAE
GIAALQSGAFHITTAGVTYFNTTPLGRAVTGTMLVSAMKDDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLRIYKPWLDQ
LFIDELGGRAEMSAFMTQAGFGYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGMKIVNPIMGVAFWKDEVEVKREEVTVR
FEEGQPVALNGKTFSNTVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQY
RDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAVTGEVTVELRRGNDYSILNTVSPNLTYKPERLSMEKVEDAPFSPL
DRIGQLTMRNLDIVDTRDKLAIYTQAGLLSAGQGASPPQLKNDERK
>Mature_445_residues
ATILQNLPAGQKVGIAFSGGLDTSAALHWMKLKGALPYAYTANLGQPDEPDYDEIPRKALQYGAEKARLIDCRSQLAAEG
IAALQSGAFHITTAGVTYFNTTPLGRAVTGTMLVSAMKDDDVNIWGDGSTFKGNDIERFYRYGLLTNPNLRIYKPWLDQL
FIDELGGRAEMSAFMTQAGFGYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGMKIVNPIMGVAFWKDEVEVKREEVTVRF
EEGQPVALNGKTFSNTVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALLFIAYERLVTGIHNEDTIEQYR
DNGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAVTGEVTVELRRGNDYSILNTVSPNLTYKPERLSMEKVEDAPFSPLD
RIGQLTMRNLDIVDTRDKLAIYTQAGLLSAGQGASPPQLKNDERK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=407, Percent_Identity=29.4840294840295, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI16950633, Length=407, Percent_Identity=29.4840294840295, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1789563, Length=446, Percent_Identity=78.2511210762332, Blast_Score=729, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324514, Length=418, Percent_Identity=28.9473684210526, Blast_Score=146, Evalue=8e-36,
Organism=Drosophila melanogaster, GI21358151, Length=392, Percent_Identity=28.8265306122449, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_POLSJ (Q12D55)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_548435.1
- ProteinModelPortal:   Q12D55
- SMR:   Q12D55
- STRING:   Q12D55
- GeneID:   4015764
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1599
- NMPDR:   fig|296591.1.peg.192
- eggNOG:   COG0137
- HOGENOM:   HBG289560
- OMA:   GGRKEMS
- PhylomeDB:   Q12D55
- ProtClustDB:   PRK05370
- BioCyc:   PSP296591:BPRO_1599-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00581
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 49569; Mature: 49438

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 43-43 BINDING 99-99 BINDING 129-129 BINDING 131-131 BINDING 131-131 BINDING 135-135 BINDING 135-135 BINDING 136-136 BINDING 136-136 BINDING 139-139 BINDING 192-192 BINDING 194-194 BINDING 201-201 BINDING 203-203 BINDING 280-280

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATILQNLPAGQKVGIAFSGGLDTSAALHWMKLKGALPYAYTANLGQPDEPDYDEIPRKA
CCCHHHHCCCCCEEEEEEECCCCHHHHHEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
LQYGAEKARLIDCRSQLAAEGIAALQSGAFHITTAGVTYFNTTPLGRAVTGTMLVSAMKD
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHCC
DDVNIWGDGSTFKGNDIERFYRYGLLTNPNLRIYKPWLDQLFIDELGGRAEMSAFMTQAG
CCCEEEECCCCCCCCCHHHHHHHCCEECCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCC
FGYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGMKIVNPIMGVAFWKDEVEVKREEVTVR
CCEEECCCHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHEEEEEEEEE
FEEGQPVALNGKTFSNTVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
ECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLVTGIHNEDTIEQYRDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAVTGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEE
TVELRRGNDYSILNTVSPNLTYKPERLSMEKVEDAPFSPLDRIGQLTMRNLDIVDTRDKL
EEEEECCCCEEEEEECCCCCEECCHHCCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCE
AIYTQAGLLSAGQGASPPQLKNDERK
EEEEECCCEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATILQNLPAGQKVGIAFSGGLDTSAALHWMKLKGALPYAYTANLGQPDEPDYDEIPRKA
CCHHHHCCCCCEEEEEEECCCCHHHHHEEHHHCCCCCEEEECCCCCCCCCCHHHHHHHH
LQYGAEKARLIDCRSQLAAEGIAALQSGAFHITTAGVTYFNTTPLGRAVTGTMLVSAMKD
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHCC
DDVNIWGDGSTFKGNDIERFYRYGLLTNPNLRIYKPWLDQLFIDELGGRAEMSAFMTQAG
CCCEEEECCCCCCCCCHHHHHHHCCEECCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHCC
FGYKMSAEKAYSTDSNMLGATHEAKDLEHLNSGMKIVNPIMGVAFWKDEVEVKREEVTVR
CCEEECCCHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHEEEEEEEEE
FEEGQPVALNGKTFSNTVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGLALL
ECCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHH
FIAYERLVTGIHNEDTIEQYRDNGRRLGRLLYQGRWFDPQAIMLRETAQRWVARAVTGEV
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEE
TVELRRGNDYSILNTVSPNLTYKPERLSMEKVEDAPFSPLDRIGQLTMRNLDIVDTRDKL
EEEEECCCCEEEEEECCCCCEECCHHCCHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCE
AIYTQAGLLSAGQGASPPQLKNDERK
EEEEECCCEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA