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The map label for this gene is recE [H]

Identifier: 91787426

GI number: 91787426

Start: 1587636

End: 1588583

Strand: Reverse

Name: recE [H]

Synonym: Bpro_1536

Alternate gene names: 91787426

Gene position: 1588583-1587636 (Counterclockwise)

Preceding gene: 91787429

Following gene: 91787424

Centisome position: 30.55

GC content: 56.22

Gene sequence:

>948_bases
ATGAATACCGAAACCGTTTTAAATTCTTCTGCATTGATGTTCGCCGCTGGCCAAGGCTACGAATCTGAAAGTACCCCTGG
CTTTGCTGCTGGCTCGCGCATGTATGCAAATTTGCCCGCTGCCCAGTACCACAGTGACAAGGAAGCGTTGAGCTGCAGCA
TGCTCAAGCCGCTGCTGGAGTCCCCTGCCCATTTCCAGGCCCACCTGCTGTCGCTCAACAAGGCCACCAAAGCCATGGAT
TTCGGCAGCCTGGTGCACGGTCTGGTACTGGAGCCTCACTTGATCGGCACTGAGTTCTCCATCTACCCTGGCATAGCGGA
CGGACGCGCAGCCGAATACAAGTCCTTCCTCACCAAACATGCAAGCCGTCTGGTGGTTGACGAGCCCACCTTTGCGCGTG
CCCGAAAGCTGGCTGAGAAAATTCTGCATCGCATCGTGTTTGGACGCCCCTTTGGCGACTTTGTGAACGAAGGCATCCCC
GAAGCCAGCATTTATGTGGAAGAGCCCACTACGGGCTTGATGCTGCGTACCCGCCTGGACCTCTACCACCCCGAATACAG
CTTCGACTTGAAGACCACCCGCCATGGCACCATCAATGCCTTCATTCGTGACGCAGTGGACCTGCACTATGACTTCCAGG
CTTTCATGTACACCATCGCCCGCTCTCTTTACGAAGGCAGGACCACTCCCGCAAAGTTTGTCTTCATCGCGGCAGAAACC
GAAGCGCCCAGCTCCATCCATCAGATCACGGCGGGGCAATCATTCATGAGCAACGGTGCGAAGAAATTTCAGGAGGTTCT
GTCTGTTTACACGGCCTGCATGCAAACCGGCTTCTGGCCTGACTCCAGTGGCAATGCAGAGGCCGAAATCGAGCCGTGGC
AGAGTTTTAGCGGCAAGTCCGACTGGAAGGCTGCGCTGGGCGGCTACCAGCTTTCTACCACGCATTAG

Upstream 100 bases:

>100_bases
TGGTCAAGACCTCATCTGTGTCCCTTGCCAAATTCTTGTCCCACCCCTTGACGTAGCGCGTTTTCGTGTTTTCATAGACA
AAAGCACCTGAGAGGTTGAT

Downstream 100 bases:

>100_bases
GCCTACTTGGCGCGGATCAGTCTCGTTTTGATTCTGTTTACCACCTTCAACATGTCGTTATCCATGTTGAAGGGTTTTGC
GAGAAAATTGGGTGTCGAAC

Product: hypothetical protein

Products: 5'-Phosphomononucleotides [C]

Alternate protein names: Exodeoxyribonuclease VIII; EXO VIII [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNTETVLNSSALMFAAGQGYESESTPGFAAGSRMYANLPAAQYHSDKEALSCSMLKPLLESPAHFQAHLLSLNKATKAMD
FGSLVHGLVLEPHLIGTEFSIYPGIADGRAAEYKSFLTKHASRLVVDEPTFARARKLAEKILHRIVFGRPFGDFVNEGIP
EASIYVEEPTTGLMLRTRLDLYHPEYSFDLKTTRHGTINAFIRDAVDLHYDFQAFMYTIARSLYEGRTTPAKFVFIAAET
EAPSSIHQITAGQSFMSNGAKKFQEVLSVYTACMQTGFWPDSSGNAEAEIEPWQSFSGKSDWKAALGGYQLSTTH

Sequences:

>Translated_315_residues
MNTETVLNSSALMFAAGQGYESESTPGFAAGSRMYANLPAAQYHSDKEALSCSMLKPLLESPAHFQAHLLSLNKATKAMD
FGSLVHGLVLEPHLIGTEFSIYPGIADGRAAEYKSFLTKHASRLVVDEPTFARARKLAEKILHRIVFGRPFGDFVNEGIP
EASIYVEEPTTGLMLRTRLDLYHPEYSFDLKTTRHGTINAFIRDAVDLHYDFQAFMYTIARSLYEGRTTPAKFVFIAAET
EAPSSIHQITAGQSFMSNGAKKFQEVLSVYTACMQTGFWPDSSGNAEAEIEPWQSFSGKSDWKAALGGYQLSTTH
>Mature_315_residues
MNTETVLNSSALMFAAGQGYESESTPGFAAGSRMYANLPAAQYHSDKEALSCSMLKPLLESPAHFQAHLLSLNKATKAMD
FGSLVHGLVLEPHLIGTEFSIYPGIADGRAAEYKSFLTKHASRLVVDEPTFARARKLAEKILHRIVFGRPFGDFVNEGIP
EASIYVEEPTTGLMLRTRLDLYHPEYSFDLKTTRHGTINAFIRDAVDLHYDFQAFMYTIARSLYEGRTTPAKFVFIAAET
EAPSSIHQITAGQSFMSNGAKKFQEVLSVYTACMQTGFWPDSSGNAEAEIEPWQSFSGKSDWKAALGGYQLSTTH

Specific function: Is involved in the recE pathway of recombination. Has a strong preference for linear duplex substrate DNA and appears to be unable to initiate degradation from single-stranded breaks in DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787612, Length=262, Percent_Identity=27.0992366412214, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010584 [H]

Pfam domain/function: PF06630 Exonuc_VIII [H]

EC number: 3.1.11.- [C]

Molecular weight: Translated: 34791; Mature: 34791

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTETVLNSSALMFAAGQGYESESTPGFAAGSRMYANLPAAQYHSDKEALSCSMLKPLLE
CCCCHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHCCCHHHHHHHHHHHHHC
SPAHFQAHLLSLNKATKAMDFGSLVHGLVLEPHLIGTEFSIYPGIADGRAAEYKSFLTKH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHHHHHHHHHHH
ASRLVVDEPTFARARKLAEKILHRIVFGRPFGDFVNEGIPEASIYVEEPTTGLMLRTRLD
HHHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCEEEEEHHH
LYHPEYSFDLKTTRHGTINAFIRDAVDLHYDFQAFMYTIARSLYEGRTTPAKFVFIAAET
HCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
EAPSSIHQITAGQSFMSNGAKKFQEVLSVYTACMQTGFWPDSSGNAEAEIEPWQSFSGKS
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHCCCCCC
DWKAALGGYQLSTTH
CHHHHHCCEEEECCC
>Mature Secondary Structure
MNTETVLNSSALMFAAGQGYESESTPGFAAGSRMYANLPAAQYHSDKEALSCSMLKPLLE
CCCCHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEECCCHHHHCCCHHHHHHHHHHHHHC
SPAHFQAHLLSLNKATKAMDFGSLVHGLVLEPHLIGTEFSIYPGIADGRAAEYKSFLTKH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHHHHHHHHHHH
ASRLVVDEPTFARARKLAEKILHRIVFGRPFGDFVNEGIPEASIYVEEPTTGLMLRTRLD
HHHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCEEEEEHHH
LYHPEYSFDLKTTRHGTINAFIRDAVDLHYDFQAFMYTIARSLYEGRTTPAKFVFIAAET
HCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
EAPSSIHQITAGQSFMSNGAKKFQEVLSVYTACMQTGFWPDSSGNAEAEIEPWQSFSGKS
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHCCCCCC
DWKAALGGYQLSTTH
CHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double-Stranded DNA [C]

Specific reaction: Degradation Of Double-Stranded DNA. It Acts Progressively In A 5' To 3' Direction, Releasing 5'-Phosphomononucleotides. [C]

General reaction: Hydrolase; Acting on ester bonds; Exodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2649487; 2199308; 8244937 [H]