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The map label for this gene is dadA2 [H]

Identifier: 91787280

GI number: 91787280

Start: 1421357

End: 1422562

Strand: Reverse

Name: dadA2 [H]

Synonym: Bpro_1385

Alternate gene names: 91787280

Gene position: 1422562-1421357 (Counterclockwise)

Preceding gene: 91787281

Following gene: 91787277

Centisome position: 27.36

GC content: 58.96

Gene sequence:

>1206_bases
ATGAAAGTCTGCGTTATCGGCGCCGGCATCGTGGGCTGCTCGACGGCCTATCAATTGGCCCGCATGGGGCATGAGGTGCA
CCTCGTGGATGAGGCCGCCGGTCCGGGCCTGGGAACGAGCAACGCCAACGGGGCCCAGCTCAGCTACAGCTATGTGGAGC
CACTGGCCTCACCGCACACCCTGGGCGGACTTCCCGGCATGTTGCTTTCACGCGGGTCGCCCCTTCGGTTCCGGCCCAGG
CTGGATTGGAGGCAGTGGTACTGGGGCTTCCAGTTTCTCAAGGCATGCACCGCAAAACAGGTTGAAAGCGGGACCCGAAC
ACTGCTGCAGCTATCGCAGCTCAGCCGCGACACGCTGATGAGATGGATGGAAAGCGAGGATTGGTCGTTTGATTACAAAA
TCAACGGGAAGCTGGTCCTTTGCCCCACCCGGGACTGCCTGAAACGCCAGGAAGCGCAGATCAAATTCCAAAGCCAGTTC
GGATGCCATCAGAAAATTCTGGGCGTGCGGGAATGCGTTGAAAAAGAACCCTCATTGCAAAGCTACTCCGGGAAATTCGC
CGGCGGCGTGTGGACTGCCGAAGAGCGGGTGGCCGACCCTTACAAGCTCTGCCAGGAGCTGCTCCGAAGCTTGGTGCGCA
TGGGTGCGAATGTTTCGTTCAATGCCAGGGTGAATGACTTTGTCACTTCGGGTGAGCGTCTGGATACCGTTCGCACCAGC
AAAGGCGACCTGAGGTCCGACGCCTTCGTGCTGGCCACCGGCGTGACGGCCCCAGCTCATGCGTCGAAACTCGGTATTTA
CCTTCCTGTTTACCCCCTCAAGGGCTACAGCATCACGCTGCCGATCAAGGGTGGGCGCCAGGCGCCAAAAGCCAGCGTCA
CGGATCTTTCTCTCAAGACAGTTTTTGCTCCCCTCGCACAAAACCTGAGAGTGGCCGCGATGGCCGAGATCGGCGGCCAT
GATCTTTCCATTCCGGGTGACCGCATCAAGCAAATGGTGGCGTCGGTTGAATCTGTTTTTCCTGGCCTATGCGATTTGTC
GTCCACTCGCCCCTGGGCGGGTTTACGGCCTGCCACGCCGACCTCCGTCCCAATCGTAGGGCGCTCCAGGTTCAAAAATC
TGTTCTTGAATGTTGGGCATGGTGCACTCGGGCTAACGCTCGCGGCAGGCAGTGCGGTAGCTATATCTCAAGAAATTTCG
TCATGA

Upstream 100 bases:

>100_bases
TGGGTGTTGGCCTTGCAGATGTGGCCTGCGCCTATCTGGCCTATGCCCGTTATACCCTATCCCCAAAAGCCCGTGAGGCT
TCAGCGCAGGAGCTCGCCGC

Downstream 100 bases:

>100_bases
CAATCAAAGAAGAACCAGCCATGCGACGGTAGGATAAAAGCTGTGCTGGGCTGGCCATCGACAATCGAACATTGCCGATA
ACTGAAATTCGAAGCAGCGC

Product: D-amino-acid dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKVCVIGAGIVGCSTAYQLARMGHEVHLVDEAAGPGLGTSNANGAQLSYSYVEPLASPHTLGGLPGMLLSRGSPLRFRPR
LDWRQWYWGFQFLKACTAKQVESGTRTLLQLSQLSRDTLMRWMESEDWSFDYKINGKLVLCPTRDCLKRQEAQIKFQSQF
GCHQKILGVRECVEKEPSLQSYSGKFAGGVWTAEERVADPYKLCQELLRSLVRMGANVSFNARVNDFVTSGERLDTVRTS
KGDLRSDAFVLATGVTAPAHASKLGIYLPVYPLKGYSITLPIKGGRQAPKASVTDLSLKTVFAPLAQNLRVAAMAEIGGH
DLSIPGDRIKQMVASVESVFPGLCDLSSTRPWAGLRPATPTSVPIVGRSRFKNLFLNVGHGALGLTLAAGSAVAISQEIS
S

Sequences:

>Translated_401_residues
MKVCVIGAGIVGCSTAYQLARMGHEVHLVDEAAGPGLGTSNANGAQLSYSYVEPLASPHTLGGLPGMLLSRGSPLRFRPR
LDWRQWYWGFQFLKACTAKQVESGTRTLLQLSQLSRDTLMRWMESEDWSFDYKINGKLVLCPTRDCLKRQEAQIKFQSQF
GCHQKILGVRECVEKEPSLQSYSGKFAGGVWTAEERVADPYKLCQELLRSLVRMGANVSFNARVNDFVTSGERLDTVRTS
KGDLRSDAFVLATGVTAPAHASKLGIYLPVYPLKGYSITLPIKGGRQAPKASVTDLSLKTVFAPLAQNLRVAAMAEIGGH
DLSIPGDRIKQMVASVESVFPGLCDLSSTRPWAGLRPATPTSVPIVGRSRFKNLFLNVGHGALGLTLAAGSAVAISQEIS
S
>Mature_401_residues
MKVCVIGAGIVGCSTAYQLARMGHEVHLVDEAAGPGLGTSNANGAQLSYSYVEPLASPHTLGGLPGMLLSRGSPLRFRPR
LDWRQWYWGFQFLKACTAKQVESGTRTLLQLSQLSRDTLMRWMESEDWSFDYKINGKLVLCPTRDCLKRQEAQIKFQSQF
GCHQKILGVRECVEKEPSLQSYSGKFAGGVWTAEERVADPYKLCQELLRSLVRMGANVSFNARVNDFVTSGERLDTVRTS
KGDLRSDAFVLATGVTAPAHASKLGIYLPVYPLKGYSITLPIKGGRQAPKASVTDLSLKTVFAPLAQNLRVAAMAEIGGH
DLSIPGDRIKQMVASVESVFPGLCDLSSTRPWAGLRPATPTSVPIVGRSRFKNLFLNVGHGALGLTLAAGSAVAISQEIS
S

Specific function: Oxidative deamination of D-amino acids [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=400, Percent_Identity=31.25, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023080
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.99.1 [H]

Molecular weight: Translated: 43491; Mature: 43491

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVCVIGAGIVGCSTAYQLARMGHEVHLVDEAAGPGLGTSNANGAQLSYSYVEPLASPHT
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHCCCCCCC
LGGLPGMLLSRGSPLRFRPRLDWRQWYWGFQFLKACTAKQVESGTRTLLQLSQLSRDTLM
CCCCCHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWMESEDWSFDYKINGKLVLCPTRDCLKRQEAQIKFQSQFGCHQKILGVRECVEKEPSLQ
HHHCCCCCEEEEEECCEEEEECCHHHHHHHHHCEEEHHHCCHHHHHHHHHHHHCCCCCCH
SYSGKFAGGVWTAEERVADPYKLCQELLRSLVRMGANVSFNARVNDFVTSGERLDTVRTS
HCCCCCCCCEECCHHHCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCHHHHEECC
KGDLRSDAFVLATGVTAPAHASKLGIYLPVYPLKGYSITLPIKGGRQAPKASVTDLSLKT
CCCCCCCCEEEEECCCCCCCHHHCEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHH
VFAPLAQNLRVAAMAEIGGHDLSIPGDRIKQMVASVESVFPGLCDLSSTRPWAGLRPATP
HHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCC
TSVPIVGRSRFKNLFLNVGHGALGLTLAAGSAVAISQEISS
CCCCCCCHHHHHHHHEECCCCCEEEEEECCCEEEEHHHHCC
>Mature Secondary Structure
MKVCVIGAGIVGCSTAYQLARMGHEVHLVDEAAGPGLGTSNANGAQLSYSYVEPLASPHT
CEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHCCCCCCC
LGGLPGMLLSRGSPLRFRPRLDWRQWYWGFQFLKACTAKQVESGTRTLLQLSQLSRDTLM
CCCCCHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWMESEDWSFDYKINGKLVLCPTRDCLKRQEAQIKFQSQFGCHQKILGVRECVEKEPSLQ
HHHCCCCCEEEEEECCEEEEECCHHHHHHHHHCEEEHHHCCHHHHHHHHHHHHCCCCCCH
SYSGKFAGGVWTAEERVADPYKLCQELLRSLVRMGANVSFNARVNDFVTSGERLDTVRTS
HCCCCCCCCEECCHHHCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCHHHHEECC
KGDLRSDAFVLATGVTAPAHASKLGIYLPVYPLKGYSITLPIKGGRQAPKASVTDLSLKT
CCCCCCCCEEEEECCCCCCCHHHCEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHH
VFAPLAQNLRVAAMAEIGGHDLSIPGDRIKQMVASVESVFPGLCDLSSTRPWAGLRPATP
HHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCC
TSVPIVGRSRFKNLFLNVGHGALGLTLAAGSAVAISQEISS
CCCCCCCHHHHHHHHEECCCCCEEEEEECCCEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]