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The map label for this gene is spoT [H]

Identifier: 91787229

GI number: 91787229

Start: 1373229

End: 1375475

Strand: Reverse

Name: spoT [H]

Synonym: Bpro_1333

Alternate gene names: 91787229

Gene position: 1375475-1373229 (Counterclockwise)

Preceding gene: 91787230

Following gene: 91787216

Centisome position: 26.45

GC content: 63.33

Gene sequence:

>2247_bases
ATGAAAGCCATGGTTTTGCCCGCCGTTTCCAGCAAGTCGACCCCGGCCGCCGCCAATGCTGCCGCGGCCAGTTTCGCTGC
ACTCACGGCCGGGCTCGACTACCTGAGCACTGCGGATATCGACAGTGTCCGGCGAGCCTACCGTTTCGCCGACGAAGCTC
ACCTGGGCCAGTTGCGCAACAGCGGTGAACCCTACATCACCCATCCGATTGCCGTCGCGCAACAGTGCGCGGAGTGGAAG
CTTGACGCCCAGGCGCTGATGGCGGCACTGCTGCACGATGCCATTGAAGACTGCGGCGTGACCAAGCCCGAACTGATCGA
GCGCTTTGGGGCGCCGGTGGCCGAGCTGGTGGATGGCCTGACCAAGCTGGAAAAGCTGGAGTTCAATACCCGCGAGGAAA
ACCAGGCCGAGTCGTTCCGCAAGATGCTGCTGGCGATGGCGCGCGATGTACGCGTCATTCTGATCAAGCTGGCCGACCGC
ACCCACAACATGCGCACGCTGGGCGATGTCCCGCGCGCCAAGTGGAGCCGCATCTCGCGCGAAACCCTGGACATCTACGC
ACCGATTGCCCACCGCCTGGGCCTGAACGCGACTTACCGGGAACTGCAAGACCTCTCCTTCCAGCACCTGCACCCCTGGC
GCTACGCGACACTGACCAAGGCCCTGCTGCGCGCCCGCGGCCGCCGGCGCGACGTGATCAAACGGGTGCAGTCCGAAGTC
GAATCGGCGTTTGCCAATGCCGGCATCCCGGTGCACATCTATGGGCGTGAGAAGACGCTGTATTCCATCTACCGCAAGAT
GGACGAAAAACACCTGTCTTTCGCCCAGGTCACCGACCTTTACGGCTTTCGGATCATCGTGCAGGACCTCACCGCCTGCT
ACACGGCCATGGGCGTTTTGCATCAGCTGTACAAGCCGCTGCCCGGAAAATTCAAGGACTATATTGCGATTCCGAAGATC
AATGGCTACCAGTCGCTGCACACCACACTGGTCGGTCCGTTCGGCACCAATATCGAGTTCCAGATGCGTACCGACGCCAT
GCATGTGGTGGCGGAGTCGGGGGTAGCCGCGCACTGGCTCTACAAGGCCACAGACCCCGAGAGTGATGCCTCCCAGCGCC
TGGGCGCCCAATGGCTGCAGTCCCTGCTGGATATCCAGCAGGAAACACGCGACGCCGCCGAGTTCTGGGACCATGTCAAG
ATCGACCTGTTTCCGGAAGCCGTCTATGTGTTCACCCCCAAGAGCCAGATCATGGCCATGCCGCGGGGTGCGACCATTGT
GGACTTCGCCTACGCCATCCACAGCGATGTCGGGCACCGCGCCGTAGCGGCCAAGGTCAACGGCGAGCAGGTCCCCCTGC
GCTCCGAGCTGCACAACGGCGACGTGGTCGAAATCATCACGGCCTCGGTCTCCAGTCCCAACCCAGGCTGGCTCGGCTTT
GTGCGCACCGGCAAGGCTCGCTCCAAAATCCGTCATCACCTCAAGACCATGGCACTGGCGGAATCGCAGGAGCTGGGTGA
AAAAATGCTGACGCAGGCCTTGCGTGCGGAAGGCATCGAAAGGGAGCGCATCCCCGAGGACGACGAAGCCCATCAGGCAA
CCTGGGAAAAAATCCTGCGCTTTACCGGCAACCGCTCACGTGCCGATTTGCTCACCGACATCGGGCTGGGCAAGCGTATC
GCAACCATGGTGGCCAAGCGCATTGTGACCTTGCTGGCAGAAACCGGCGAAAAACCCGACGCCCTGCTGATGAGCCGTGA
ACGCTATACCGCCCATGAGTCGGTATCACAGGGTGCGCTGCTGCTTGATGGCAGTGAGGGCGCCTCGGTGCAGTTTGCGC
CCTGTTGTCGGCCCATCCCGGGCGATGGCATTGTCGGCTACCTGGGCCGGGGTGAGGGGCTGGTCGTGCATACGGACGAC
TGCGCCGTGGCCAAGCGCCTGCAGCACCGCGACAGCGAGCGCTTCATTGCCGTGGACTGGTCCGACGAACCGGTGCGCAC
ATTTGAGACCAATCTTCTGGTGACGGTGGCCAACGGCAAAGGAGTTCTGGCCCGGGTGGCCGCCGCGCTGGCCAGCGCCG
AAGCCGACATCACGCATGTTGATATGGGGCAGGAAGCCGCGCAGGATGCAACCGACCTGCGCTTTGGGGTGGCGGTGCGG
GACCGTGTTCACCTGGCATCGGCGCTGCGCAGCGTCAAGCGCACCCCCTCCGTGCTGCGGGTGCAGCGCGCCAAGGCGGC
GGGGTGA

Upstream 100 bases:

>100_bases
CCTGTTGTTGAAAAGCACCGTGACCCGGTGCTTTTTTCTTTTTGGGCGCCGTAAAATCCGTTTTTTTCATGTTTTATTCA
TGCCGCGATACATTTAAGGC

Downstream 100 bases:

>100_bases
AATTGGCCCCCACGCTTGCTCACTTCGTGTAGCCGCTGCCCCCCGAGGGGGCCGCTGCGCCTGCGGCCCGGCTAAGCCGG
TTCCGCGGCCCCTGCTGGGC

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MKAMVLPAVSSKSTPAAANAAAASFAALTAGLDYLSTADIDSVRRAYRFADEAHLGQLRNSGEPYITHPIAVAQQCAEWK
LDAQALMAALLHDAIEDCGVTKPELIERFGAPVAELVDGLTKLEKLEFNTREENQAESFRKMLLAMARDVRVILIKLADR
THNMRTLGDVPRAKWSRISRETLDIYAPIAHRLGLNATYRELQDLSFQHLHPWRYATLTKALLRARGRRRDVIKRVQSEV
ESAFANAGIPVHIYGREKTLYSIYRKMDEKHLSFAQVTDLYGFRIIVQDLTACYTAMGVLHQLYKPLPGKFKDYIAIPKI
NGYQSLHTTLVGPFGTNIEFQMRTDAMHVVAESGVAAHWLYKATDPESDASQRLGAQWLQSLLDIQQETRDAAEFWDHVK
IDLFPEAVYVFTPKSQIMAMPRGATIVDFAYAIHSDVGHRAVAAKVNGEQVPLRSELHNGDVVEIITASVSSPNPGWLGF
VRTGKARSKIRHHLKTMALAESQELGEKMLTQALRAEGIERERIPEDDEAHQATWEKILRFTGNRSRADLLTDIGLGKRI
ATMVAKRIVTLLAETGEKPDALLMSRERYTAHESVSQGALLLDGSEGASVQFAPCCRPIPGDGIVGYLGRGEGLVVHTDD
CAVAKRLQHRDSERFIAVDWSDEPVRTFETNLLVTVANGKGVLARVAAALASAEADITHVDMGQEAAQDATDLRFGVAVR
DRVHLASALRSVKRTPSVLRVQRAKAAG

Sequences:

>Translated_748_residues
MKAMVLPAVSSKSTPAAANAAAASFAALTAGLDYLSTADIDSVRRAYRFADEAHLGQLRNSGEPYITHPIAVAQQCAEWK
LDAQALMAALLHDAIEDCGVTKPELIERFGAPVAELVDGLTKLEKLEFNTREENQAESFRKMLLAMARDVRVILIKLADR
THNMRTLGDVPRAKWSRISRETLDIYAPIAHRLGLNATYRELQDLSFQHLHPWRYATLTKALLRARGRRRDVIKRVQSEV
ESAFANAGIPVHIYGREKTLYSIYRKMDEKHLSFAQVTDLYGFRIIVQDLTACYTAMGVLHQLYKPLPGKFKDYIAIPKI
NGYQSLHTTLVGPFGTNIEFQMRTDAMHVVAESGVAAHWLYKATDPESDASQRLGAQWLQSLLDIQQETRDAAEFWDHVK
IDLFPEAVYVFTPKSQIMAMPRGATIVDFAYAIHSDVGHRAVAAKVNGEQVPLRSELHNGDVVEIITASVSSPNPGWLGF
VRTGKARSKIRHHLKTMALAESQELGEKMLTQALRAEGIERERIPEDDEAHQATWEKILRFTGNRSRADLLTDIGLGKRI
ATMVAKRIVTLLAETGEKPDALLMSRERYTAHESVSQGALLLDGSEGASVQFAPCCRPIPGDGIVGYLGRGEGLVVHTDD
CAVAKRLQHRDSERFIAVDWSDEPVRTFETNLLVTVANGKGVLARVAAALASAEADITHVDMGQEAAQDATDLRFGVAVR
DRVHLASALRSVKRTPSVLRVQRAKAAG
>Mature_748_residues
MKAMVLPAVSSKSTPAAANAAAASFAALTAGLDYLSTADIDSVRRAYRFADEAHLGQLRNSGEPYITHPIAVAQQCAEWK
LDAQALMAALLHDAIEDCGVTKPELIERFGAPVAELVDGLTKLEKLEFNTREENQAESFRKMLLAMARDVRVILIKLADR
THNMRTLGDVPRAKWSRISRETLDIYAPIAHRLGLNATYRELQDLSFQHLHPWRYATLTKALLRARGRRRDVIKRVQSEV
ESAFANAGIPVHIYGREKTLYSIYRKMDEKHLSFAQVTDLYGFRIIVQDLTACYTAMGVLHQLYKPLPGKFKDYIAIPKI
NGYQSLHTTLVGPFGTNIEFQMRTDAMHVVAESGVAAHWLYKATDPESDASQRLGAQWLQSLLDIQQETRDAAEFWDHVK
IDLFPEAVYVFTPKSQIMAMPRGATIVDFAYAIHSDVGHRAVAAKVNGEQVPLRSELHNGDVVEIITASVSSPNPGWLGF
VRTGKARSKIRHHLKTMALAESQELGEKMLTQALRAEGIERERIPEDDEAHQATWEKILRFTGNRSRADLLTDIGLGKRI
ATMVAKRIVTLLAETGEKPDALLMSRERYTAHESVSQGALLLDGSEGASVQFAPCCRPIPGDGIVGYLGRGEGLVVHTDD
CAVAKRLQHRDSERFIAVDWSDEPVRTFETNLLVTVANGKGVLARVAAALASAEADITHVDMGQEAAQDATDLRFGVAVR
DRVHLASALRSVKRTPSVLRVQRAKAAG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=712, Percent_Identity=44.9438202247191, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI1789147, Length=682, Percent_Identity=32.8445747800587, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 82726; Mature: 82726

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMVLPAVSSKSTPAAANAAAASFAALTAGLDYLSTADIDSVRRAYRFADEAHLGQLRN
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHC
SGEPYITHPIAVAQQCAEWKLDAQALMAALLHDAIEDCGVTKPELIERFGAPVAELVDGL
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHH
TKLEKLEFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRTLGDVPRAKWSRISR
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHCCCHHHHHHHHH
ETLDIYAPIAHRLGLNATYRELQDLSFQHLHPWRYATLTKALLRARGRRRDVIKRVQSEV
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ESAFANAGIPVHIYGREKTLYSIYRKMDEKHLSFAQVTDLYGFRIIVQDLTACYTAMGVL
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQLYKPLPGKFKDYIAIPKINGYQSLHTTLVGPFGTNIEFQMRTDAMHVVAESGVAAHWL
HHHHCCCCCCCCCEEECCCCCCCHHHHHHEECCCCCCEEEEEECHHHHHHHHCCCEEEEE
YKATDPESDASQRLGAQWLQSLLDIQQETRDAAEFWDHVKIDLFPEAVYVFTPKSQIMAM
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCHHHHEEC
PRGATIVDFAYAIHSDVGHRAVAAKVNGEQVPLRSELHNGDVVEIITASVSSPNPGWLGF
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEEEEEECCCCCCCCEEEE
VRTGKARSKIRHHLKTMALAESQELGEKMLTQALRAEGIERERIPEDDEAHQATWEKILR
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHH
FTGNRSRADLLTDIGLGKRIATMVAKRIVTLLAETGEKPDALLMSRERYTAHESVSQGAL
HCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCEE
LLDGSEGASVQFAPCCRPIPGDGIVGYLGRGEGLVVHTDDCAVAKRLQHRDSERFIAVDW
EEECCCCCCEEECCCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEEEEC
SDEPVRTFETNLLVTVANGKGVLARVAAALASAEADITHVDMGQEAAQDATDLRFGVAVR
CCCCCCEEECEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHH
DRVHLASALRSVKRTPSVLRVQRAKAAG
HHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKAMVLPAVSSKSTPAAANAAAASFAALTAGLDYLSTADIDSVRRAYRFADEAHLGQLRN
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHC
SGEPYITHPIAVAQQCAEWKLDAQALMAALLHDAIEDCGVTKPELIERFGAPVAELVDGL
CCCCEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHH
TKLEKLEFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRTLGDVPRAKWSRISR
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHCCCHHHHHHHHH
ETLDIYAPIAHRLGLNATYRELQDLSFQHLHPWRYATLTKALLRARGRRRDVIKRVQSEV
HHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ESAFANAGIPVHIYGREKTLYSIYRKMDEKHLSFAQVTDLYGFRIIVQDLTACYTAMGVL
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HQLYKPLPGKFKDYIAIPKINGYQSLHTTLVGPFGTNIEFQMRTDAMHVVAESGVAAHWL
HHHHCCCCCCCCCEEECCCCCCCHHHHHHEECCCCCCEEEEEECHHHHHHHHCCCEEEEE
YKATDPESDASQRLGAQWLQSLLDIQQETRDAAEFWDHVKIDLFPEAVYVFTPKSQIMAM
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEECCHHHHEEC
PRGATIVDFAYAIHSDVGHRAVAAKVNGEQVPLRSELHNGDVVEIITASVSSPNPGWLGF
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHCCCCCEEEEEEECCCCCCCCEEEE
VRTGKARSKIRHHLKTMALAESQELGEKMLTQALRAEGIERERIPEDDEAHQATWEKILR
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHH
FTGNRSRADLLTDIGLGKRIATMVAKRIVTLLAETGEKPDALLMSRERYTAHESVSQGAL
HCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCEE
LLDGSEGASVQFAPCCRPIPGDGIVGYLGRGEGLVVHTDDCAVAKRLQHRDSERFIAVDW
EEECCCCCCEEECCCCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHCCCCCEEEEEC
SDEPVRTFETNLLVTVANGKGVLARVAAALASAEADITHVDMGQEAAQDATDLRFGVAVR
CCCCCCEEECEEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCHHHHCCHHHHHHHHHHH
DRVHLASALRSVKRTPSVLRVQRAKAAG
HHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]