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The map label for this gene is hemL

Identifier: 91787031

GI number: 91787031

Start: 1171497

End: 1172825

Strand: Reverse

Name: hemL

Synonym: Bpro_1133

Alternate gene names: 91787031

Gene position: 1172825-1171497 (Counterclockwise)

Preceding gene: 91787032

Following gene: 91787027

Centisome position: 22.55

GC content: 64.79

Gene sequence:

>1329_bases
ATGGAACCACAAAACATTAAGCCCCACACCCCCGAGCTCTCCCGCAACACCGCGCTGTTCGAGCGCGCCAAAAAGTTGAT
CCCCGGCGGGGTGAATTCGCCGGTCAGGGCGTTCAAGGCCGTGGGAGGTACGCCGCGCTTTGTGCAGCGCGCCCAGGGCG
CCTACTTCTGGGACGCCGACGACCAGCGCTACATCGACTACATCGGCTCGTGGGGGCCAATGATTCTGGGGCACGGGCAT
CCCGCCGTGCTTGCGTCCGTTCAGAAAGCGCTGCTGGACGGTTTCTCTTATGGTGCGCCGACCGAGCGCGAAGTGGAACT
GGCTGAAGAGCTCGTGCGCCTGGTGCCGTCCCTCGAAATGGTGCGGCTGGTGAGCTCGGGTACCGAAGCAGCCATGAGCG
CCATCCGCCTGGCGCGCGGCGCGACCGGACGCAGCAAGATCATCAAGTTCGAGGGCTGCTACCACGGCCACGCCGACGCC
CTGCTGGTGAAAGCCGGCTCCGGCCTGGCCACCTTCGGCAACCCAACCAGCGCCGGTGTACCGCCCGAGGTAGTGCAGCA
CACCCTGGTGCTCGAATACAACCACCTGGCCCAGCTGGAAGAAGCTTTTGCGCTGCATGGCAGCGCGATTGCCTGCCTGA
TGATCGAGCCGATCGCCGGCAACATGAATTTCGTGCGGGCCAGCATTCCTTTCATGCAGCGTTGCCGCGAGCTGTGCACC
CAGTATGGTGCGCTGCTGGTGTTTGACGAAGTCATGACCGGCTTTCGCGTGGCCCTCGGTGGCGCGCAAAGCGTGTACGC
CAAAAGTATTCCCGGCTTCAAACCCGACATGACGGTGCTGGGCAAGGTGATTGGCGGCGGCATGCCGCTGGCGGCCTTTG
GCGGAACCCGCGCCGTGATGGAACAGCTGGCGCCTTTGGGCCCGGTCTACCAGGCCGGTACGCTGTCGGGCAACCCGGTG
GCCACCGCCTGCGGCCTGGCCACCTTGCGCGAGATCCAGAAACCCGGGTTTTATGAAGCACTGGGAGAGCGCACGCGCGG
TCTGGTCGCAGGCCTCACGCAGGCGGCCAACAAGGCGGGCGTGCCTTTTTGCGGTGACAGCGAGGGCGGCATGTTCGGCT
TTTTCCTGCTCGGGCAACTGCCTCAAAACTACGCCACGGTCATGACGACCGACGGTCCGGCCTTCAACCGCTTCTTTCAT
GCCATGCTGGCCAGCGGGGTGTACTACGCGCCGGCGCTGTACGAGGCCGGCTTTGTCAGCTCGGCGCACAGCGCGGCGGA
TATTGAGGCCACGGTGGATGCAGCGGCGAAGTTTTTTGCCGGGGCCTGA

Upstream 100 bases:

>100_bases
GCGCAGGCCGATGTCGATGATGAAGAATCCGAAGACGAAGCGGGCGACACGCCCGACGATGCCGCACTGACATCCAAGCC
TCCTTTTGACCTGCCGCCCA

Downstream 100 bases:

>100_bases
ACTGGCCTCATGAGGTGAGGCGCTCTTTCGCTGAATGCCCGGCGGATTAATCTGCTCCGGAGCCGGCGGCCCCAATATCA
AAATGGCCCGCGCCACCCGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MEPQNIKPHTPELSRNTALFERAKKLIPGGVNSPVRAFKAVGGTPRFVQRAQGAYFWDADDQRYIDYIGSWGPMILGHGH
PAVLASVQKALLDGFSYGAPTEREVELAEELVRLVPSLEMVRLVSSGTEAAMSAIRLARGATGRSKIIKFEGCYHGHADA
LLVKAGSGLATFGNPTSAGVPPEVVQHTLVLEYNHLAQLEEAFALHGSAIACLMIEPIAGNMNFVRASIPFMQRCRELCT
QYGALLVFDEVMTGFRVALGGAQSVYAKSIPGFKPDMTVLGKVIGGGMPLAAFGGTRAVMEQLAPLGPVYQAGTLSGNPV
ATACGLATLREIQKPGFYEALGERTRGLVAGLTQAANKAGVPFCGDSEGGMFGFFLLGQLPQNYATVMTTDGPAFNRFFH
AMLASGVYYAPALYEAGFVSSAHSAADIEATVDAAAKFFAGA

Sequences:

>Translated_442_residues
MEPQNIKPHTPELSRNTALFERAKKLIPGGVNSPVRAFKAVGGTPRFVQRAQGAYFWDADDQRYIDYIGSWGPMILGHGH
PAVLASVQKALLDGFSYGAPTEREVELAEELVRLVPSLEMVRLVSSGTEAAMSAIRLARGATGRSKIIKFEGCYHGHADA
LLVKAGSGLATFGNPTSAGVPPEVVQHTLVLEYNHLAQLEEAFALHGSAIACLMIEPIAGNMNFVRASIPFMQRCRELCT
QYGALLVFDEVMTGFRVALGGAQSVYAKSIPGFKPDMTVLGKVIGGGMPLAAFGGTRAVMEQLAPLGPVYQAGTLSGNPV
ATACGLATLREIQKPGFYEALGERTRGLVAGLTQAANKAGVPFCGDSEGGMFGFFLLGQLPQNYATVMTTDGPAFNRFFH
AMLASGVYYAPALYEAGFVSSAHSAADIEATVDAAAKFFAGA
>Mature_442_residues
MEPQNIKPHTPELSRNTALFERAKKLIPGGVNSPVRAFKAVGGTPRFVQRAQGAYFWDADDQRYIDYIGSWGPMILGHGH
PAVLASVQKALLDGFSYGAPTEREVELAEELVRLVPSLEMVRLVSSGTEAAMSAIRLARGATGRSKIIKFEGCYHGHADA
LLVKAGSGLATFGNPTSAGVPPEVVQHTLVLEYNHLAQLEEAFALHGSAIACLMIEPIAGNMNFVRASIPFMQRCRELCT
QYGALLVFDEVMTGFRVALGGAQSVYAKSIPGFKPDMTVLGKVIGGGMPLAAFGGTRAVMEQLAPLGPVYQAGTLSGNPV
ATACGLATLREIQKPGFYEALGERTRGLVAGLTQAANKAGVPFCGDSEGGMFGFFLLGQLPQNYATVMTTDGPAFNRFFH
AMLASGVYYAPALYEAGFVSSAHSAADIEATVDAAAKFFAGA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=299, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI284507298, Length=218, Percent_Identity=27.0642201834862, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786349, Length=429, Percent_Identity=57.3426573426573, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1789016, Length=374, Percent_Identity=32.0855614973262, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788044, Length=321, Percent_Identity=28.9719626168224, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787560, Length=333, Percent_Identity=30.03003003003, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789759, Length=320, Percent_Identity=29.0625, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693181, Length=308, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786991, Length=300, Percent_Identity=28.3333333333333, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=353, Percent_Identity=26.628895184136, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=148, Percent_Identity=30.4054054054054, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323470, Length=332, Percent_Identity=23.4939759036145, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324432, Length=331, Percent_Identity=24.773413897281, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21357415, Length=325, Percent_Identity=26.7692307692308, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_POLSJ (Q12EF7)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547983.1
- ProteinModelPortal:   Q12EF7
- SMR:   Q12EF7
- STRING:   Q12EF7
- GeneID:   4011871
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_1133
- NMPDR:   fig|296591.1.peg.3270
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   Q12EF7
- ProtClustDB:   PRK00062
- BioCyc:   PSP296591:BPRO_1133-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46774; Mature: 46774

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPQNIKPHTPELSRNTALFERAKKLIPGGVNSPVRAFKAVGGTPRFVQRAQGAYFWDAD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCEEECCC
DQRYIDYIGSWGPMILGHGHPAVLASVQKALLDGFSYGAPTEREVELAEELVRLVPSLEM
CCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHH
VRLVSSGTEAAMSAIRLARGATGRSKIIKFEGCYHGHADALLVKAGSGLATFGNPTSAGV
HHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCCCCCCC
PPEVVQHTLVLEYNHLAQLEEAFALHGSAIACLMIEPIAGNMNFVRASIPFMQRCRELCT
CHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHH
QYGALLVFDEVMTGFRVALGGAQSVYAKSIPGFKPDMTVLGKVIGGGMPLAAFGGTRAVM
HHCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHCCCHHHHH
EQLAPLGPVYQAGTLSGNPVATACGLATLREIQKPGFYEALGERTRGLVAGLTQAANKAG
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
VPFCGDSEGGMFGFFLLGQLPQNYATVMTTDGPAFNRFFHAMLASGVYYAPALYEAGFVS
CCCCCCCCCCEEHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
SAHSAADIEATVDAAAKFFAGA
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEPQNIKPHTPELSRNTALFERAKKLIPGGVNSPVRAFKAVGGTPRFVQRAQGAYFWDAD
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCEEECCC
DQRYIDYIGSWGPMILGHGHPAVLASVQKALLDGFSYGAPTEREVELAEELVRLVPSLEM
CCHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHH
VRLVSSGTEAAMSAIRLARGATGRSKIIKFEGCYHGHADALLVKAGSGLATFGNPTSAGV
HHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEECCCCCEECCCCCCCCC
PPEVVQHTLVLEYNHLAQLEEAFALHGSAIACLMIEPIAGNMNFVRASIPFMQRCRELCT
CHHHHHHHHHEEHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCHHHHHHHHHHH
QYGALLVFDEVMTGFRVALGGAQSVYAKSIPGFKPDMTVLGKVIGGGMPLAAFGGTRAVM
HHCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHCCCHHHHH
EQLAPLGPVYQAGTLSGNPVATACGLATLREIQKPGFYEALGERTRGLVAGLTQAANKAG
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
VPFCGDSEGGMFGFFLLGQLPQNYATVMTTDGPAFNRFFHAMLASGVYYAPALYEAGFVS
CCCCCCCCCCEEHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
SAHSAADIEATVDAAAKFFAGA
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA