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The map label for this gene is metG

Identifier: 91786826

GI number: 91786826

Start: 942189

End: 944258

Strand: Reverse

Name: metG

Synonym: Bpro_0924

Alternate gene names: 91786826

Gene position: 944258-942189 (Counterclockwise)

Preceding gene: 91786831

Following gene: 91786824

Centisome position: 18.16

GC content: 62.08

Gene sequence:

>2070_bases
ATGTCCCAGCGCCGCCTTTTCGTCACCACCGCCCTTCCCTACGCCAACGGGAATTTTCACATTGGCCACATCATGGAATA
CATCCAGGCCGACATCTGGGTGCGCTACCAGAGGATGCAGGGCCATGAGGTGAACTTTGTCGGCGCCGACGACGCGCACG
GCGCACCCATCATGATCGCCGCCGAAAAAGCGGGCAAGACACCGCAGCAGTTCGTGGCCGATATCGCCGCGGGCCGCAAG
CCTTACCTCGACGGCTTTCACATCAGTTTTGACAACTGGCATTCGACCGACGGGCCGGAAAATCACCAGCTGGCGCAGCA
GATCTATCTGGACCTCAAAAAGGCCGGGCTGATCGAAACCAAAACCGTCGAGCAGTTCTTCGACCCCGACAAGAACATGT
TCCTGCCCGACCGCTTCATCAAGGGCCAGTGCCCCAAATGTGGCGCGAAAGACCAGTACGGCGACAACTGCGAGGTATGC
GGCGCGGTCTATGCGCCGACCGACCTGATCAACCCGTACTCGGCCCTGTCGGGCGCCACGCCCGTGCTCAAAAGCTCGGA
ACATTTTTTCTTCAGGCTTTCCGACCCGCGCTGCGTGGCCTTCCTGGAGCAGTGGACCCAGGACGGCATGCTGCAGACCG
AAGTTGCCAACAAGGTCAAGGAATGGTTTTCGCCACGCCAGAACGTCGACGGCACCACCAGCGAAGGCCTGGACGACTGG
GACATCAGCCGCGACGCGCCTTATTTCGGCATCGAGATCCCGGATGCACCGGGCAAGTATTTCTACGTGTGGCTGGACGC
CCCGGTCGGCTACCTGGCCTCGCTGAAAAACCTGCTGGACAAGAAGGGCCTGGACTACGATGCCTACATGGCCGATCCGG
GGCTGGAGCAGTACCACTTCATCGGCAAGGACATCATCACCTTCCACACCCTGTTCTGGCCGGCCATGCTGCATTTCAGC
GGCCGCAAGACGCCGGACAACGTCTTTGTGCACGGCTTCCTGACCGTCAACAACGGCGAAAAAATGAGCAAGAGCCGCGG
TACCGGGCTGGACCCGCTCAAGTACCTCAACCTGGGCATGAATCCCGAATGGCTGCGCTACTACCTCGCCGCCAAGCTCA
ACGCCCGCAATGAAGACATCGACTTCAATCCCGAAGACTTCATGGCGCGCGTCAATGCCGACCTGGTCGGCAAATACATC
AACATTGCCAGCCGTGCGGCCGGATTCATCAGCAAGCGGTTTGACGGCCGGCTCGGCGAGCCGTCCGCTGATGGCGATGC
CCTGCTTGAAGTGTTGCGGGGCGCCTCGCCGGCGATCCAGGAGCTCTATGCCGAACGCGAATATGGCAAGGCCCTGCGCG
AGGTCATGCAACTGGCCGATCGTGTCAACGGCTATGTCGACCAGAACAAGCCCTGGGAGCTGGCCAAGCAGCCAGGCATG
GAAGCCCGGCTCCTCGACGTCTGCACGGTCTGCATCGAGGCTTTCCGCCTGCTGACCATCTACCTCAAGCCGGTGCTGCC
CGCGCTGGGCACCCAGGTGGAAGACTTCCTGAAAACCGGTTCGCTGACGTTCCGGGACGCGGGAGCCAGCCTCGGTAGTG
GCCACGTGATCGGCGAGTACAAGCACCTGATGCAGCGCGTCGACGTGAAGCAGCTGGACGCGCTGTTCGAGCCCCCGGCC
GCTGCGCCCGAGCCGGCACAGCTCACCCCCGGCGGGGAAGCGCTGGCGGCCCCGATCACCATCGACGATTTCGCGAAGGT
AGACCTGCGCATTGCAAAGATCGTCAACTGCGAAGCCGTGGAAGGCTCCACCAAGCTGCTGCGCCTGACGCTGGATGTGG
GTGAAGGCAAGATGCGCAACGTCTTCAGCGGCATCGCCTCGGCCTATACGCCCGACCAGCTGATCGGCAAGCACACGGTG
GTGGTGGCCAACCTGGCGCCGCGCAAAATGAAGTTTGGTGTCAGCGAAGGCATGGTGCTGGCCGCCAGCCACGCTGACGA
GAAAGCCCAGCCCGGCATTTATGTGCTGGAGCCGCTGCCTGGCGCATCACCGGGTTTGCGCGTTCGCTGA

Upstream 100 bases:

>100_bases
CGGGACCGAAATGCCCCGAAAACACCCGGTCGCGGGGTGGGAGCGGCAAAGGCCGTCAGGCGACTTAAAATCATGGGTTA
GCCCCACCAAAGCACCCACC

Downstream 100 bases:

>100_bases
GCCCGACAGACTGCACTAGGGTTTGTGCTTCATCGCAGCGTGCGGGGCATCCGGCGCGCTGACAAACCGCAGTGCCGTGC
CGGCGTGCCCTGCAAGCTCG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSQRRLFVTTALPYANGNFHIGHIMEYIQADIWVRYQRMQGHEVNFVGADDAHGAPIMIAAEKAGKTPQQFVADIAAGRK
PYLDGFHISFDNWHSTDGPENHQLAQQIYLDLKKAGLIETKTVEQFFDPDKNMFLPDRFIKGQCPKCGAKDQYGDNCEVC
GAVYAPTDLINPYSALSGATPVLKSSEHFFFRLSDPRCVAFLEQWTQDGMLQTEVANKVKEWFSPRQNVDGTTSEGLDDW
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKKGLDYDAYMADPGLEQYHFIGKDIITFHTLFWPAMLHFS
GRKTPDNVFVHGFLTVNNGEKMSKSRGTGLDPLKYLNLGMNPEWLRYYLAAKLNARNEDIDFNPEDFMARVNADLVGKYI
NIASRAAGFISKRFDGRLGEPSADGDALLEVLRGASPAIQELYAEREYGKALREVMQLADRVNGYVDQNKPWELAKQPGM
EARLLDVCTVCIEAFRLLTIYLKPVLPALGTQVEDFLKTGSLTFRDAGASLGSGHVIGEYKHLMQRVDVKQLDALFEPPA
AAPEPAQLTPGGEALAAPITIDDFAKVDLRIAKIVNCEAVEGSTKLLRLTLDVGEGKMRNVFSGIASAYTPDQLIGKHTV
VVANLAPRKMKFGVSEGMVLAASHADEKAQPGIYVLEPLPGASPGLRVR

Sequences:

>Translated_689_residues
MSQRRLFVTTALPYANGNFHIGHIMEYIQADIWVRYQRMQGHEVNFVGADDAHGAPIMIAAEKAGKTPQQFVADIAAGRK
PYLDGFHISFDNWHSTDGPENHQLAQQIYLDLKKAGLIETKTVEQFFDPDKNMFLPDRFIKGQCPKCGAKDQYGDNCEVC
GAVYAPTDLINPYSALSGATPVLKSSEHFFFRLSDPRCVAFLEQWTQDGMLQTEVANKVKEWFSPRQNVDGTTSEGLDDW
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKKGLDYDAYMADPGLEQYHFIGKDIITFHTLFWPAMLHFS
GRKTPDNVFVHGFLTVNNGEKMSKSRGTGLDPLKYLNLGMNPEWLRYYLAAKLNARNEDIDFNPEDFMARVNADLVGKYI
NIASRAAGFISKRFDGRLGEPSADGDALLEVLRGASPAIQELYAEREYGKALREVMQLADRVNGYVDQNKPWELAKQPGM
EARLLDVCTVCIEAFRLLTIYLKPVLPALGTQVEDFLKTGSLTFRDAGASLGSGHVIGEYKHLMQRVDVKQLDALFEPPA
AAPEPAQLTPGGEALAAPITIDDFAKVDLRIAKIVNCEAVEGSTKLLRLTLDVGEGKMRNVFSGIASAYTPDQLIGKHTV
VVANLAPRKMKFGVSEGMVLAASHADEKAQPGIYVLEPLPGASPGLRVR
>Mature_688_residues
SQRRLFVTTALPYANGNFHIGHIMEYIQADIWVRYQRMQGHEVNFVGADDAHGAPIMIAAEKAGKTPQQFVADIAAGRKP
YLDGFHISFDNWHSTDGPENHQLAQQIYLDLKKAGLIETKTVEQFFDPDKNMFLPDRFIKGQCPKCGAKDQYGDNCEVCG
AVYAPTDLINPYSALSGATPVLKSSEHFFFRLSDPRCVAFLEQWTQDGMLQTEVANKVKEWFSPRQNVDGTTSEGLDDWD
ISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKKGLDYDAYMADPGLEQYHFIGKDIITFHTLFWPAMLHFSG
RKTPDNVFVHGFLTVNNGEKMSKSRGTGLDPLKYLNLGMNPEWLRYYLAAKLNARNEDIDFNPEDFMARVNADLVGKYIN
IASRAAGFISKRFDGRLGEPSADGDALLEVLRGASPAIQELYAEREYGKALREVMQLADRVNGYVDQNKPWELAKQPGME
ARLLDVCTVCIEAFRLLTIYLKPVLPALGTQVEDFLKTGSLTFRDAGASLGSGHVIGEYKHLMQRVDVKQLDALFEPPAA
APEPAQLTPGGEALAAPITIDDFAKVDLRIAKIVNCEAVEGSTKLLRLTLDVGEGKMRNVFSGIASAYTPDQLIGKHTVV
VANLAPRKMKFGVSEGMVLAASHADEKAQPGIYVLEPLPGASPGLRVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=652, Percent_Identity=27.9141104294479, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI24308436, Length=535, Percent_Identity=25.7943925233645, Blast_Score=149, Evalue=7e-36,
Organism=Escherichia coli, GI1788432, Length=697, Percent_Identity=54.232424677188, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1786861, Length=152, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17540794, Length=594, Percent_Identity=27.6094276094276, Blast_Score=214, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI212645096, Length=527, Percent_Identity=25.9962049335863, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71992781, Length=355, Percent_Identity=27.887323943662, Blast_Score=112, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=573, Percent_Identity=27.5741710296684, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321610, Length=583, Percent_Identity=24.8713550600343, Blast_Score=123, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24655311, Length=521, Percent_Identity=28.4069097888676, Blast_Score=201, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21358483, Length=531, Percent_Identity=26.1770244821092, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI21355409, Length=153, Percent_Identity=32.0261437908497, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_POLSJ (Q12F12)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_547778.1
- ProteinModelPortal:   Q12F12
- STRING:   Q12F12
- GeneID:   4011039
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_0924
- NMPDR:   fig|296591.1.peg.3011
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- PhylomeDB:   Q12F12
- ProtClustDB:   PRK00133
- BioCyc:   PSP296591:BPRO_0924-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76389; Mature: 76258

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 344-344

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRRLFVTTALPYANGNFHIGHIMEYIQADIWVRYQRMQGHEVNFVGADDAHGAPIMIA
CCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHEEHHCCCCEEEEECCCCCCCCEEEEE
AEKAGKTPQQFVADIAAGRKPYLDGFHISFDNWHSTDGPENHQLAQQIYLDLKKAGLIET
ECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
KTVEQFFDPDKNMFLPDRFIKGQCPKCGAKDQYGDNCEVCGAVYAPTDLINPYSALSGAT
HHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCHHHHHCCCC
PVLKSSEHFFFRLSDPRCVAFLEQWTQDGMLQTEVANKVKEWFSPRQNVDGTTSEGLDDW
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKKGLDYDAYMADPGLEQYHF
CCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHH
IGKDIITFHTLFWPAMLHFSGRKTPDNVFVHGFLTVNNGEKMSKSRGTGLDPLKYLNLGM
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHHCCCCCCCHHHHHCCCC
NPEWLRYYLAAKLNARNEDIDFNPEDFMARVNADLVGKYINIASRAAGFISKRFDGRLGE
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSADGDALLEVLRGASPAIQELYAEREYGKALREVMQLADRVNGYVDQNKPWELAKQPGM
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
EARLLDVCTVCIEAFRLLTIYLKPVLPALGTQVEDFLKTGSLTFRDAGASLGSGHVIGEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEECCCCCCCCCCCHHHH
KHLMQRVDVKQLDALFEPPAAAPEPAQLTPGGEALAAPITIDDFAKVDLRIAKIVNCEAV
HHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHEEEEHEECCEEC
EGSTKLLRLTLDVGEGKMRNVFSGIASAYTPDQLIGKHTVVVANLAPRKMKFGVSEGMVL
CCCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCHHCCCCCCCEEE
AASHADEKAQPGIYVLEPLPGASPGLRVR
EECCCCCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SQRRLFVTTALPYANGNFHIGHIMEYIQADIWVRYQRMQGHEVNFVGADDAHGAPIMIA
CCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHHEEHHCCCCEEEEECCCCCCCCEEEEE
AEKAGKTPQQFVADIAAGRKPYLDGFHISFDNWHSTDGPENHQLAQQIYLDLKKAGLIET
ECCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCH
KTVEQFFDPDKNMFLPDRFIKGQCPKCGAKDQYGDNCEVCGAVYAPTDLINPYSALSGAT
HHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCHHHHHCCCC
PVLKSSEHFFFRLSDPRCVAFLEQWTQDGMLQTEVANKVKEWFSPRQNVDGTTSEGLDDW
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLKNLLDKKGLDYDAYMADPGLEQYHF
CCCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHH
IGKDIITFHTLFWPAMLHFSGRKTPDNVFVHGFLTVNNGEKMSKSRGTGLDPLKYLNLGM
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHHCCCCCCCHHHHHCCCC
NPEWLRYYLAAKLNARNEDIDFNPEDFMARVNADLVGKYINIASRAAGFISKRFDGRLGE
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PSADGDALLEVLRGASPAIQELYAEREYGKALREVMQLADRVNGYVDQNKPWELAKQPGM
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCC
EARLLDVCTVCIEAFRLLTIYLKPVLPALGTQVEDFLKTGSLTFRDAGASLGSGHVIGEY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEECCCCCCCCCCCHHHH
KHLMQRVDVKQLDALFEPPAAAPEPAQLTPGGEALAAPITIDDFAKVDLRIAKIVNCEAV
HHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHEEEEHEECCEEC
EGSTKLLRLTLDVGEGKMRNVFSGIASAYTPDQLIGKHTVVVANLAPRKMKFGVSEGMVL
CCCCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHCCCEEEEEECCCCHHCCCCCCCEEE
AASHADEKAQPGIYVLEPLPGASPGLRVR
EECCCCCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA