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The map label for this gene is nreB [H]

Identifier: 91786612

GI number: 91786612

Start: 720561

End: 722222

Strand: Direct

Name: nreB [H]

Synonym: Bpro_0708

Alternate gene names: 91786612

Gene position: 720561-722222 (Clockwise)

Preceding gene: 91786611

Following gene: 91786613

Centisome position: 13.86

GC content: 64.26

Gene sequence:

>1662_bases
ATGGAACAGGTGCCCACTTTTCTCCTCCGTTTGACGGGTGTCACCTTGTTGTATGCCGCCGCGGCAATAGGAGGGCTGAT
GTATGCCGTTGTCGGCAGCACGGTCACGCTGGTCTGGGCGCCGAGCGGGATCGCGCTGGCTGCACTCCTGGCCTACGGCT
ACCGCCTGTCTTTCGGCGTTGCGCTGGGCGCCTTTCTGGCGAATGCCTGGACCGGCATACCGCTTGCCGCCTCCGCAGTC
ATCGCCATGGGGAACACTCTGGAGGCCTTGGTCGGCGCATTTCTCCTGGTTCGCCTGGTCCGCTTTCGCAATGCCCTGGA
CCGACGTCGGGATGTATTCGCATTAATCGTGCTGGCCGCCATTTTCAGCACGATGTTGAGCGCTTCTGTGGGGGTGGCAA
CCCTGGCCCTGGGCGGCATTGTGTCCTTTGGAGAGTACGCCGCGGTGTGGTTGAAATGGTGGCTTGGGGACATGATGGGC
GTGCTGGTGGTAACCCCGCCACTGCTGCTCTGGCTCAGCCATTCCCGCCCTGTCCTGTCACCCCTGAAAGCCGTCGAGGC
CTTGTGTCTGGTCGCGGCACTCGTGCTTGTCAGCCACAAAATCTTTGGTGCGCCGGAATTGGCAGGCCATGGCTACTATC
CGGCCTCACTTGCCGTGTTCCCTTTCGTCATTTGGGGGGCGCTGCGCTTTGGTCGATGGGGCGCCAGCCTGGTCACGCTG
GTTGTCTCCGTGCTGGCCATTTGGGGCACCACACAAGGAACAGGGCCATTCGTGGTCGATCAGCCCGTGGACAGCCTGGT
GCGCTGGTGCGCGTTCGCGATTGTCGTGGCGGTGACCGGCCTCCTGCTGGCCGCATCCGTGGCCGAACAGCGGCGCGCGC
AGGCGGATCTCAAAAGCTCGCACGACGGCCTGGAGCAACGCGTGAAGGAGCGCACCCAGGCTATCGCCGACATCAACGCC
GATCTGCGGCGGGAAATGGCGGCGCGTCGGCGCCTGGAGAAAGCGCTGATCCGGGTCAGCGAAGAACAGCAGCAAGCCAT
CGGCCGGGAATTGCACGATGGCCTGGGTCAACACCTGACCGGCCTCGCCCTCTTCAGTGCGACCTTGCAGCAAAAGCTCC
ACGAGCGCGCGCAACCGGAGGCCGAAGCCGCGCGTCGGATCGTCGAGTTGGTCAATCAGGCGACAGCCATGACCCGCTCC
GTGGCCAGGGGCCTCTACCCGGCGGCACTGGAGGTCGGCGGCCTGTCCGCCGCGCTGGAGCAGCTGGCGGAGTACACCCG
CTCGCTGCAAGGCATGGCCTGCGTTTTCCGCTGCGGTGCGGACGTGCAGGTGCGCGACCCGCTGGTCGCCATCAACCTGT
ACCGGGTCGCCCAGGAAGCCGTCAATAATGCGGTGAAATACAGCCAGGCCAACCATTTGCGGATAGACCTGACCCGCGTC
GAGGACAGGCACCGGCTCGCCATCAGCGATGACGGCATCGGTGCCGACCCCGAGCGCATCTGGCATGGCCAGGGCTTGGG
AATGCACAGCATGCGTTATCGGGCGAGCCTGCTCGGCGGTACCTTTGCCATCGAGAGAAATGCCCACAGAGGCACCACCG
TCGCCGTCATGTATCCGGACCAGGGGGAACAGAATGAACAACAACGACAACACGGCTCGTAG

Upstream 100 bases:

>100_bases
CAACCGCCCTCGCGGCCTAGCCGATGCGGAATATCTCAAGCTGAAATTTATCAACTCGGTACCTCGGCTTGAACCAGCCC
GGATCGCTTCATGGAGAACA

Downstream 100 bases:

>100_bases
GCAGGAGAAGACCCGAATCATGATGGTGGACGACCATCCCCTCGTGCGGGAGGGCATGGCCCGGTTTCTCAATCTGCAGG
GGGATCTCGATCTGTGCTGC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MEQVPTFLLRLTGVTLLYAAAAIGGLMYAVVGSTVTLVWAPSGIALAALLAYGYRLSFGVALGAFLANAWTGIPLAASAV
IAMGNTLEALVGAFLLVRLVRFRNALDRRRDVFALIVLAAIFSTMLSASVGVATLALGGIVSFGEYAAVWLKWWLGDMMG
VLVVTPPLLLWLSHSRPVLSPLKAVEALCLVAALVLVSHKIFGAPELAGHGYYPASLAVFPFVIWGALRFGRWGASLVTL
VVSVLAIWGTTQGTGPFVVDQPVDSLVRWCAFAIVVAVTGLLLAASVAEQRRAQADLKSSHDGLEQRVKERTQAIADINA
DLRREMAARRRLEKALIRVSEEQQQAIGRELHDGLGQHLTGLALFSATLQQKLHERAQPEAEAARRIVELVNQATAMTRS
VARGLYPAALEVGGLSAALEQLAEYTRSLQGMACVFRCGADVQVRDPLVAINLYRVAQEAVNNAVKYSQANHLRIDLTRV
EDRHRLAISDDGIGADPERIWHGQGLGMHSMRYRASLLGGTFAIERNAHRGTTVAVMYPDQGEQNEQQRQHGS

Sequences:

>Translated_553_residues
MEQVPTFLLRLTGVTLLYAAAAIGGLMYAVVGSTVTLVWAPSGIALAALLAYGYRLSFGVALGAFLANAWTGIPLAASAV
IAMGNTLEALVGAFLLVRLVRFRNALDRRRDVFALIVLAAIFSTMLSASVGVATLALGGIVSFGEYAAVWLKWWLGDMMG
VLVVTPPLLLWLSHSRPVLSPLKAVEALCLVAALVLVSHKIFGAPELAGHGYYPASLAVFPFVIWGALRFGRWGASLVTL
VVSVLAIWGTTQGTGPFVVDQPVDSLVRWCAFAIVVAVTGLLLAASVAEQRRAQADLKSSHDGLEQRVKERTQAIADINA
DLRREMAARRRLEKALIRVSEEQQQAIGRELHDGLGQHLTGLALFSATLQQKLHERAQPEAEAARRIVELVNQATAMTRS
VARGLYPAALEVGGLSAALEQLAEYTRSLQGMACVFRCGADVQVRDPLVAINLYRVAQEAVNNAVKYSQANHLRIDLTRV
EDRHRLAISDDGIGADPERIWHGQGLGMHSMRYRASLLGGTFAIERNAHRGTTVAVMYPDQGEQNEQQRQHGS
>Mature_553_residues
MEQVPTFLLRLTGVTLLYAAAAIGGLMYAVVGSTVTLVWAPSGIALAALLAYGYRLSFGVALGAFLANAWTGIPLAASAV
IAMGNTLEALVGAFLLVRLVRFRNALDRRRDVFALIVLAAIFSTMLSASVGVATLALGGIVSFGEYAAVWLKWWLGDMMG
VLVVTPPLLLWLSHSRPVLSPLKAVEALCLVAALVLVSHKIFGAPELAGHGYYPASLAVFPFVIWGALRFGRWGASLVTL
VVSVLAIWGTTQGTGPFVVDQPVDSLVRWCAFAIVVAVTGLLLAASVAEQRRAQADLKSSHDGLEQRVKERTQAIADINA
DLRREMAARRRLEKALIRVSEEQQQAIGRELHDGLGQHLTGLALFSATLQQKLHERAQPEAEAARRIVELVNQATAMTRS
VARGLYPAALEVGGLSAALEQLAEYTRSLQGMACVFRCGADVQVRDPLVAINLYRVAQEAVNNAVKYSQANHLRIDLTRV
EDRHRLAISDDGIGADPERIWHGQGLGMHSMRYRASLLGGTFAIERNAHRGTTVAVMYPDQGEQNEQQRQHGS

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG3851

COG function: function code T; Signal transduction histidine kinase, glucose-6-phosphate specific

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1787474, Length=216, Percent_Identity=30.5555555555556, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788812, Length=211, Percent_Identity=27.9620853080569, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59703; Mature: 59703

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHCCC
>Mature Secondary Structure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CCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]