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The map label for this gene is alkK [H]

Identifier: 91786587

GI number: 91786587

Start: 696273

End: 697925

Strand: Direct

Name: alkK [H]

Synonym: Bpro_0683

Alternate gene names: 91786587

Gene position: 696273-697925 (Clockwise)

Preceding gene: 91786586

Following gene: 91786588

Centisome position: 13.39

GC content: 59.35

Gene sequence:

>1653_bases
ATGAGTGCAAAAGCCTACGCAGAGGGCCTTGACAGGAATGCCGCCAACCATGTACCTCTGACGCCACTAAATTTTCTTGA
TCGTACGGCTGACGTTTTTCCGCATCGAACCGCCATCATCCATGGAACCTTCCGCCAAACCTGGGCTGAAACACGCGACC
GTTGTTACCGTCTCGCTTCTGCGCTCGTCAAGTTGGGAATACAAGCCGGCGACACAGTGTCGATCATCGCGCCGAACACG
CCCGCCATGCTGGAGGCTCACTTCGGCGTTCCTTTGTCAGGGGCCGTACTCAATGCCATCAACTGTCGCCTTGACGCTGA
CGGGATTGCTTTCATCTTGCGGCACGGTGAGTGCAAACTGTTGTTGGTGGATCGTGAATTTTCGCCGCTGGTAGTTAAAG
CCCTTCAAGGCGTGTCCAACCCACCCCGTGTGATCGATATCAACGATCTCGAGGCGCCTGCCGGCGCCGCCATCGGCGAG
ACGGATTACGAGTCATTTTTGGCCGGCGGAGACCCGGCATTCCCCGGCCGTTGGCCAACTGATGAGTGGACACCGATCGC
GCTCAACTACACGTCAGGCACGACGGGTGACCCCAAAGGCGTGGTGCCAAGCCACCGGGGTACCTACCTCATGAGTCTCC
TGCAGATGACCGACTGGGCACTGCCGCGCGCGCCGGTCTATTTGTGGACGCTGCCGATGTTCCATGCCAATGGCTGGTGT
TTTACCTGGGCGATCACCGCAGCGGCAGGCACGCATGTGTGCCTCCGCAAGGTGACGGCGGCTAATGTGTTCACTGCCAT
TTCGGATCACGGTGTTGATCACTTCTGCGCAGCACCGATAGTGATGGCTGGCATTGCCAGTACACCTGAGGCGGAGCGCC
GGCCGCTGCCGCGCCGCGTGCGTGTATTGACAGCCGGGTCTCCTCCGCCGGCTGCTGTTCTTGAAGCTGTCAGGGCCATG
GGCTTTGATGTGGACCACGTCTTCGGCATTACCGAGATCTCCGGCACTCCGGTTAGTTGTGCTTGGCAGACCGAATGGGA
CGAGCTACCCGCTGACCAGCAAGGTCGCCTCCAGGCACGTCAAGGCGTGCGTGCAGCAGCCCTGGAGAATCTCCGGGTTG
CTGACCCTGATACGCTCGAACCCGTACCAAGAGATGGCGTCGCCACGGGCGAAATTCTGATACGTGGCAACACCGTGATG
ATGGGCTATCTGAAAAACCCTGGCGCTACCGCCAAGGCTTTTGCGGGTGGCTGGTTCCACACGGGCGACGTGGCCGTTGT
GCATCCGGACGGCTATATGCAGATCACGGACCGTTCGAAGGACATCATTATTTCTGGCGGAGAAAACATATCGTCGGTAG
AAGTGGAGGAGGTTCTGTACCGGCTCGCCGGTGTGCTGAATGCCGCGGTAGTCGCGCAGCCTGACGACAAATGGGGCGAA
GTGCCCTGTGCCTTCGTTGAACTCAAACCGGATGCGCCGCTCCTCACTGAGGTCGATGTGATCTCATTCTGCCGTGACCG
CCTGGCCCATTTCAAGTGCCCCCGTCGCGTGGTATTTGCAGAGTTGCCGAAGACTGCCACGGGCAAGATTCAGAAGTTCC
GCTTGCGCGAGCTGGCTGGCAGCCGCAATGCGATTACACAGTTGGCTGCATGA

Upstream 100 bases:

>100_bases
ACGCAGATCCCAGCCGCATTGAGAACTCACTCGTTACCGACCGTGAGGTGGTCAGCAGTTTCACCACTTTTCACGAAACC
ACCTACAAGGAGACAGAGCA

Downstream 100 bases:

>100_bases
GTGTAGGGCTGCGAAGGGGTACCCGCTGCGGGGAGGTCGGGTCCGCCCTGGGCGGCGGATTCAGCGGCCGCTGAATGCGA
TCTGGTAGCCAATCCCTGCC

Product: AMP-dependent synthetase and ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSAKAYAEGLDRNAANHVPLTPLNFLDRTADVFPHRTAIIHGTFRQTWAETRDRCYRLASALVKLGIQAGDTVSIIAPNT
PAMLEAHFGVPLSGAVLNAINCRLDADGIAFILRHGECKLLLVDREFSPLVVKALQGVSNPPRVIDINDLEAPAGAAIGE
TDYESFLAGGDPAFPGRWPTDEWTPIALNYTSGTTGDPKGVVPSHRGTYLMSLLQMTDWALPRAPVYLWTLPMFHANGWC
FTWAITAAAGTHVCLRKVTAANVFTAISDHGVDHFCAAPIVMAGIASTPEAERRPLPRRVRVLTAGSPPPAAVLEAVRAM
GFDVDHVFGITEISGTPVSCAWQTEWDELPADQQGRLQARQGVRAAALENLRVADPDTLEPVPRDGVATGEILIRGNTVM
MGYLKNPGATAKAFAGGWFHTGDVAVVHPDGYMQITDRSKDIIISGGENISSVEVEEVLYRLAGVLNAAVVAQPDDKWGE
VPCAFVELKPDAPLLTEVDVISFCRDRLAHFKCPRRVVFAELPKTATGKIQKFRLRELAGSRNAITQLAA

Sequences:

>Translated_550_residues
MSAKAYAEGLDRNAANHVPLTPLNFLDRTADVFPHRTAIIHGTFRQTWAETRDRCYRLASALVKLGIQAGDTVSIIAPNT
PAMLEAHFGVPLSGAVLNAINCRLDADGIAFILRHGECKLLLVDREFSPLVVKALQGVSNPPRVIDINDLEAPAGAAIGE
TDYESFLAGGDPAFPGRWPTDEWTPIALNYTSGTTGDPKGVVPSHRGTYLMSLLQMTDWALPRAPVYLWTLPMFHANGWC
FTWAITAAAGTHVCLRKVTAANVFTAISDHGVDHFCAAPIVMAGIASTPEAERRPLPRRVRVLTAGSPPPAAVLEAVRAM
GFDVDHVFGITEISGTPVSCAWQTEWDELPADQQGRLQARQGVRAAALENLRVADPDTLEPVPRDGVATGEILIRGNTVM
MGYLKNPGATAKAFAGGWFHTGDVAVVHPDGYMQITDRSKDIIISGGENISSVEVEEVLYRLAGVLNAAVVAQPDDKWGE
VPCAFVELKPDAPLLTEVDVISFCRDRLAHFKCPRRVVFAELPKTATGKIQKFRLRELAGSRNAITQLAA
>Mature_549_residues
SAKAYAEGLDRNAANHVPLTPLNFLDRTADVFPHRTAIIHGTFRQTWAETRDRCYRLASALVKLGIQAGDTVSIIAPNTP
AMLEAHFGVPLSGAVLNAINCRLDADGIAFILRHGECKLLLVDREFSPLVVKALQGVSNPPRVIDINDLEAPAGAAIGET
DYESFLAGGDPAFPGRWPTDEWTPIALNYTSGTTGDPKGVVPSHRGTYLMSLLQMTDWALPRAPVYLWTLPMFHANGWCF
TWAITAAAGTHVCLRKVTAANVFTAISDHGVDHFCAAPIVMAGIASTPEAERRPLPRRVRVLTAGSPPPAAVLEAVRAMG
FDVDHVFGITEISGTPVSCAWQTEWDELPADQQGRLQARQGVRAAALENLRVADPDTLEPVPRDGVATGEILIRGNTVMM
GYLKNPGATAKAFAGGWFHTGDVAVVHPDGYMQITDRSKDIIISGGENISSVEVEEVLYRLAGVLNAAVVAQPDDKWGEV
PCAFVELKPDAPLLTEVDVISFCRDRLAHFKCPRRVVFAELPKTATGKIQKFRLRELAGSRNAITQLAA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=577, Percent_Identity=27.9029462738302, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI187761345, Length=557, Percent_Identity=26.2118491921005, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI187761343, Length=557, Percent_Identity=26.2118491921005, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI157311624, Length=489, Percent_Identity=25.1533742331288, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI157311622, Length=489, Percent_Identity=25.1533742331288, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI58082049, Length=489, Percent_Identity=25.1533742331288, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI122937307, Length=517, Percent_Identity=24.7582205029014, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI38505220, Length=516, Percent_Identity=25.3875968992248, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI42544132, Length=489, Percent_Identity=23.721881390593, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=517, Percent_Identity=25.1450676982592, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI42794756, Length=483, Percent_Identity=23.3954451345756, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI42794760, Length=483, Percent_Identity=23.3954451345756, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI42794758, Length=483, Percent_Identity=23.3954451345756, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI221142682, Length=524, Percent_Identity=27.4809160305344, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI145693145, Length=527, Percent_Identity=27.5142314990512, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788107, Length=540, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=27.6717557251908, Blast_Score=122, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71994703, Length=592, Percent_Identity=26.8581081081081, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71994694, Length=592, Percent_Identity=26.8581081081081, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71994690, Length=592, Percent_Identity=26.8581081081081, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17559526, Length=542, Percent_Identity=25.4612546125461, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17560308, Length=538, Percent_Identity=25.2788104089219, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=448, Percent_Identity=28.5714285714286, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=26.1627906976744, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=519, Percent_Identity=24.4701348747592, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=560, Percent_Identity=24.8214285714286, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=519, Percent_Identity=24.4701348747592, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=531, Percent_Identity=25.0470809792844, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=491, Percent_Identity=22.6069246435845, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=514, Percent_Identity=23.9299610894942, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319699, Length=362, Percent_Identity=27.9005524861878, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24581924, Length=555, Percent_Identity=25.4054054054054, Blast_Score=140, Evalue=3e-33,
Organism=Drosophila melanogaster, GI21355181, Length=508, Percent_Identity=27.1653543307087, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859661, Length=529, Percent_Identity=27.2211720226843, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24648260, Length=501, Percent_Identity=24.5508982035928, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24656500, Length=520, Percent_Identity=26.7307692307692, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19922652, Length=513, Percent_Identity=24.9512670565302, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21356947, Length=517, Percent_Identity=23.9845261121857, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24653035, Length=517, Percent_Identity=22.0502901353965, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648257, Length=482, Percent_Identity=24.4813278008299, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281365686, Length=474, Percent_Identity=23.2067510548523, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI161076582, Length=523, Percent_Identity=21.7973231357553, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17933690, Length=95, Percent_Identity=38.9473684210526, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20130357, Length=512, Percent_Identity=21.6796875, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59413; Mature: 59281

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKAYAEGLDRNAANHVPLTPLNFLDRTADVFPHRTAIIHGTFRQTWAETRDRCYRLAS
CCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHH
ALVKLGIQAGDTVSIIAPNTPAMLEAHFGVPLSGAVLNAINCRLDADGIAFILRHGECKL
HHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHEEEEECCCCEEEEEECCCEEE
LLVDREFSPLVVKALQGVSNPPRVIDINDLEAPAGAAIGETDYESFLAGGDPAFPGRWPT
EEECCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
DEWTPIALNYTSGTTGDPKGVVPSHRGTYLMSLLQMTDWALPRAPVYLWTLPMFHANGWC
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEHCCCCEE
FTWAITAAAGTHVCLRKVTAANVFTAISDHGVDHFCAAPIVMAGIASTPEAERRPLPRRV
EEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCEE
RVLTAGSPPPAAVLEAVRAMGFDVDHVFGITEISGTPVSCAWQTEWDELPADQQGRLQAR
EEEECCCCCHHHHHHHHHHHCCCHHHHEEEEEECCCCEEEEECCCHHHCCCCCCCHHHHH
QGVRAAALENLRVADPDTLEPVPRDGVATGEILIRGNTVMMGYLKNPGATAKAFAGGWFH
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCHHHHCCCCEE
TGDVAVVHPDGYMQITDRSKDIIISGGENISSVEVEEVLYRLAGVLNAAVVAQPDDKWGE
CCCEEEECCCCEEEEECCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHEEEECCCCCCCC
VPCAFVELKPDAPLLTEVDVISFCRDRLAHFKCPRRVVFAELPKTATGKIQKFRLRELAG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
SRNAITQLAA
CCHHHHHHCC
>Mature Secondary Structure 
SAKAYAEGLDRNAANHVPLTPLNFLDRTADVFPHRTAIIHGTFRQTWAETRDRCYRLAS
CCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHH
ALVKLGIQAGDTVSIIAPNTPAMLEAHFGVPLSGAVLNAINCRLDADGIAFILRHGECKL
HHHHHCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHEEEEECCCCEEEEEECCCEEE
LLVDREFSPLVVKALQGVSNPPRVIDINDLEAPAGAAIGETDYESFLAGGDPAFPGRWPT
EEECCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCC
DEWTPIALNYTSGTTGDPKGVVPSHRGTYLMSLLQMTDWALPRAPVYLWTLPMFHANGWC
CCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEHCCCCEE
FTWAITAAAGTHVCLRKVTAANVFTAISDHGVDHFCAAPIVMAGIASTPEAERRPLPRRV
EEEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCEE
RVLTAGSPPPAAVLEAVRAMGFDVDHVFGITEISGTPVSCAWQTEWDELPADQQGRLQAR
EEEECCCCCHHHHHHHHHHHCCCHHHHEEEEEECCCCEEEEECCCHHHCCCCCCCHHHHH
QGVRAAALENLRVADPDTLEPVPRDGVATGEILIRGNTVMMGYLKNPGATAKAFAGGWFH
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCHHHHCCCCEE
TGDVAVVHPDGYMQITDRSKDIIISGGENISSVEVEEVLYRLAGVLNAAVVAQPDDKWGE
CCCEEEECCCCEEEEECCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHEEEECCCCCCCC
VPCAFVELKPDAPLLTEVDVISFCRDRLAHFKCPRRVVFAELPKTATGKIQKFRLRELAG
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCC
SRNAITQLAA
CCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]