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The map label for this gene is bcr [H]

Identifier: 91786299

GI number: 91786299

Start: 402105

End: 403334

Strand: Reverse

Name: bcr [H]

Synonym: Bpro_0389

Alternate gene names: 91786299

Gene position: 403334-402105 (Counterclockwise)

Preceding gene: 91786300

Following gene: 91786297

Centisome position: 7.76

GC content: 63.33

Gene sequence:

>1230_bases
ATGAACCCGAACGCCGACCAACTCTGGCGCGCGCCGCGCTGGACGCTGGCCGTCCTGCTGGCAGTCCTCGGCATGCTGGG
GCCGTTTTCGATCGACACCTACATTCCGGCCTTCTCGGGCATTGCGACGGCGCTGGGCGCCTCGCCGGTCGAGATGCAGC
AAACGCTGTCGGCCTACCTGTTCGGTTTTGCCTTCATGAACCTGTTTCATGGTGCGCTGGCCGACAGCTTTGGCCGGCGG
CCAGTGGTGCTGTGGGGGATTGCGGTGTTCACCCTGGCGTCGGCCGGCTGCGCCTTGTCGCAGAGCATTGGCCAGCTGGT
TTTTTTCAGGGCGCTGCAGGGCCTCTCCACGGGCGCCGGCATTGTGGTGTCGCGTGCCGTGATACGCGACATGTACCCGC
CCGCGCAGGCTCAGCAGGTCATGAGCCAGGTCACCATCTATTTCGGAGTCGCGCCTGCAATTGCGCCCCTTATGGGCGGC
TGGCTGTTTGTGCACCTCGGCTGGCACAGCATTTTCTGGTTTCTCACGGGCGTCGGCGCAACGCTCTGGGCCGCCAACTT
CCGGTTTTTGCCGGAAACATTGCGCAGCGAGGAGCGCCAGCCCTTCGAAGTCAGGCACCTGATGCGCGGCTACTGGCAGC
TGGGCTCCAGTCCGCGCTTTTTGCTGCTGGCGCTGGCCAGCGGCGTGCCTTTCAATGGCATGTTCCTCTATGTGCTGGCG
GCCCCGGCGTTCCTCGGTCAACACCTGTCCCTGCAACCCACCGAATTTTTCTGGTTTTTCATTCTCACCATTTCCGGCAT
CATGTCGGGCGCCTGGCTGAGCGGCAGGCTGGCCGGAAAAATTGCACCCAAACGGCAGATACGGCACGGCTTTGTCATCA
TGCTGGTGACGTCGCTGGTCAACCTGGCCGCCAACCTGCTGTTCGCGCCGCATGTTTCCTGGGCGCTGTTCCCGATCGCC
GTATTTGCCTTTGGCTGGGCACTGATGGTGCCGGTCGTCACACTGCTGGTGCTGGACCTTTATCCCGAGCGGCGCGGCAT
GGCTTCCTCCCTGCAAGCCTTCATCGGTTCAACGGCCAACGGTCTGGTGGCGGGCCTGATTGCGCCGCTGGTCATGCACT
CGACGCCCGCGCTGGCGACTGTTTCACTGGGCATGATGTGCATCGGGCTGGTGTCGTGGGCGTATTTGCACCATCGCTGG
CCGGAAATCGGCCGTGCGGTGTCCCTATAG

Upstream 100 bases:

>100_bases
TGGCCGAACTGCCGCTGAAAACCGCCGTCAAAATTGCCGCCGAGATCACCGGTGCGCCGCGCAACGAGCTGTATGACACC
GCGCTCAAACTGAAGAAGGC

Downstream 100 bases:

>100_bases
CCCGTACCTGCTACCCGCCGCTGCAGTGAACGGCACGGATCAGTTGTTGTCGTTCATCCTGATCGGGATTTCCATTCCCT
GCGAGGTGCGCCGCACCACA

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MNPNADQLWRAPRWTLAVLLAVLGMLGPFSIDTYIPAFSGIATALGASPVEMQQTLSAYLFGFAFMNLFHGALADSFGRR
PVVLWGIAVFTLASAGCALSQSIGQLVFFRALQGLSTGAGIVVSRAVIRDMYPPAQAQQVMSQVTIYFGVAPAIAPLMGG
WLFVHLGWHSIFWFLTGVGATLWAANFRFLPETLRSEERQPFEVRHLMRGYWQLGSSPRFLLLALASGVPFNGMFLYVLA
APAFLGQHLSLQPTEFFWFFILTISGIMSGAWLSGRLAGKIAPKRQIRHGFVIMLVTSLVNLAANLLFAPHVSWALFPIA
VFAFGWALMVPVVTLLVLDLYPERRGMASSLQAFIGSTANGLVAGLIAPLVMHSTPALATVSLGMMCIGLVSWAYLHHRW
PEIGRAVSL

Sequences:

>Translated_409_residues
MNPNADQLWRAPRWTLAVLLAVLGMLGPFSIDTYIPAFSGIATALGASPVEMQQTLSAYLFGFAFMNLFHGALADSFGRR
PVVLWGIAVFTLASAGCALSQSIGQLVFFRALQGLSTGAGIVVSRAVIRDMYPPAQAQQVMSQVTIYFGVAPAIAPLMGG
WLFVHLGWHSIFWFLTGVGATLWAANFRFLPETLRSEERQPFEVRHLMRGYWQLGSSPRFLLLALASGVPFNGMFLYVLA
APAFLGQHLSLQPTEFFWFFILTISGIMSGAWLSGRLAGKIAPKRQIRHGFVIMLVTSLVNLAANLLFAPHVSWALFPIA
VFAFGWALMVPVVTLLVLDLYPERRGMASSLQAFIGSTANGLVAGLIAPLVMHSTPALATVSLGMMCIGLVSWAYLHHRW
PEIGRAVSL
>Mature_409_residues
MNPNADQLWRAPRWTLAVLLAVLGMLGPFSIDTYIPAFSGIATALGASPVEMQQTLSAYLFGFAFMNLFHGALADSFGRR
PVVLWGIAVFTLASAGCALSQSIGQLVFFRALQGLSTGAGIVVSRAVIRDMYPPAQAQQVMSQVTIYFGVAPAIAPLMGG
WLFVHLGWHSIFWFLTGVGATLWAANFRFLPETLRSEERQPFEVRHLMRGYWQLGSSPRFLLLALASGVPFNGMFLYVLA
APAFLGQHLSLQPTEFFWFFILTISGIMSGAWLSGRLAGKIAPKRQIRHGFVIMLVTSLVNLAANLLFAPHVSWALFPIA
VFAFGWALMVPVVTLLVLDLYPERRGMASSLQAFIGSTANGLVAGLIAPLVMHSTPALATVSLGMMCIGLVSWAYLHHRW
PEIGRAVSL

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=347, Percent_Identity=36.0230547550432, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI48994889, Length=390, Percent_Identity=27.9487179487179, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87082312, Length=355, Percent_Identity=29.0140845070423, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790146, Length=333, Percent_Identity=26.7267267267267, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790794, Length=316, Percent_Identity=25.3164556962025, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790195, Length=149, Percent_Identity=34.8993288590604, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787065, Length=275, Percent_Identity=25.8181818181818, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081738, Length=180, Percent_Identity=31.6666666666667, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788392, Length=172, Percent_Identity=33.1395348837209, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789042, Length=143, Percent_Identity=29.3706293706294, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=185, Percent_Identity=28.1081081081081, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322070, Length=128, Percent_Identity=33.59375, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322071, Length=127, Percent_Identity=33.0708661417323, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44544; Mature: 44544

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]