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The map label for this gene is braC [H]

Identifier: 91786260

GI number: 91786260

Start: 368186

End: 369316

Strand: Direct

Name: braC [H]

Synonym: Bpro_0350

Alternate gene names: 91786260

Gene position: 368186-369316 (Clockwise)

Preceding gene: 91786259

Following gene: 91786261

Centisome position: 7.08

GC content: 54.64

Gene sequence:

>1131_bases
ATGTTTGCGACCAGATTCTCAAAGTTACAAGCCTGCGTGATGTTTGCTGGGGTGGCGCTTGCCTTCTCAGCCGGAGCGCA
AGTGAAAGTGGGTGTATTTGTCCCTCTTACGGGAGATGCGGCCGGGTACGGCCAGAGTGCTTTGGAGGCGATTCAGCTTG
CAGTTAAACAGAAAAATGATGCCGGCGGGGTGCTCGGCCAAAAGATCGAATTGGTAGTGCGAGACGATGGCGCCAAGCCC
GAGCAATCGGTAACCGTTGCCAAGCGCTTGGCCACGAGCGATGGAGTACTGGTCATGCTGGGTAGCATCTCCAGCCCTGC
CAGCTTTGCGGCAACACAAGTGGCGCTTCAGACCGGCACGCCGCAAATCGTGTTGTTGGGTACCGCTCAGCGGATTACCA
CCCAAGGCAACCCATGGGTCTTTCGTGGAACCATCCCCGATACGAAGCTGTCGGGAGATCTGGTTGAGTTCATCATCCGT
AAATATCCGGGCAAGAAGAAAGTCGGCTACCTGTACGTGAACGACGATTTTGGTCGTGGCGGCTTTGAGGCTTTCAAGGC
ACGTGCTGAAAGCTCCGGACTGACTATCGTCGCGGAGGAAAAGTACGCTCCAGGTGACTTGGACTTCACCAGCCAACTGA
CCAAGATTCAGAGCGAAAATCCGGACGTATTTGTTGATTGGTCCCGCTACACTGAAGGCGCGCTGGTGGCGATTCAGGCC
AAGCGCAAGGGCATGACATTGCCGCACTTCGGCTCAGAGGGACTCTCGCACCCCAACTTCATCAAACTTGCCGGGGACGC
AAGCGACGGCATGATGTATCCCGTGCCTTTCAGCATGACCACGCAAAGCGGAAACAACATCGCCGTCACCTTGGCGCAGG
ATATCAAGAAGACCTACAACAAGGAGATTGAGTACACGCATGCCTTGGCCTATGACGCCATGCGGGCTGTCATCTTCGCG
ATTGAGAAATCCGGCACTACCACCGATAAGCTGAAATTCAGGGACGCCCTGAAGAGCCTGGAATTTGATTCAACCCGTGG
AAAGTTCAAATTTGACGCGAAAGGCGATCCCACCTTGCTGCCTTCGATCGTCAAACTCTCAAGTGGTAAAGAGCAAGACG
CCAACAAGTAA

Upstream 100 bases:

>100_bases
TCCTCTGGCGAAGGCGGGTGTCTATGAAAAAGTGAGCGTGACGGGATTCCTCAAAAAGAGGGGTTGAGTAAGTTCGATCA
ATTGAAAAAGGACCATTGAT

Downstream 100 bases:

>100_bases
ACGACGAAGGAAACAGTCATGAAAAAAGAATTTATCCGCGGAACCTGGCAGAAGACGCGTGCCTTCTCTCCTGCGGTGAT
CACAGAGGGCGGAAAAACGA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFATRFSKLQACVMFAGVALAFSAGAQVKVGVFVPLTGDAAGYGQSALEAIQLAVKQKNDAGGVLGQKIELVVRDDGAKP
EQSVTVAKRLATSDGVLVMLGSISSPASFAATQVALQTGTPQIVLLGTAQRITTQGNPWVFRGTIPDTKLSGDLVEFIIR
KYPGKKKVGYLYVNDDFGRGGFEAFKARAESSGLTIVAEEKYAPGDLDFTSQLTKIQSENPDVFVDWSRYTEGALVAIQA
KRKGMTLPHFGSEGLSHPNFIKLAGDASDGMMYPVPFSMTTQSGNNIAVTLAQDIKKTYNKEIEYTHALAYDAMRAVIFA
IEKSGTTTDKLKFRDALKSLEFDSTRGKFKFDAKGDPTLLPSIVKLSSGKEQDANK

Sequences:

>Translated_376_residues
MFATRFSKLQACVMFAGVALAFSAGAQVKVGVFVPLTGDAAGYGQSALEAIQLAVKQKNDAGGVLGQKIELVVRDDGAKP
EQSVTVAKRLATSDGVLVMLGSISSPASFAATQVALQTGTPQIVLLGTAQRITTQGNPWVFRGTIPDTKLSGDLVEFIIR
KYPGKKKVGYLYVNDDFGRGGFEAFKARAESSGLTIVAEEKYAPGDLDFTSQLTKIQSENPDVFVDWSRYTEGALVAIQA
KRKGMTLPHFGSEGLSHPNFIKLAGDASDGMMYPVPFSMTTQSGNNIAVTLAQDIKKTYNKEIEYTHALAYDAMRAVIFA
IEKSGTTTDKLKFRDALKSLEFDSTRGKFKFDAKGDPTLLPSIVKLSSGKEQDANK
>Mature_376_residues
MFATRFSKLQACVMFAGVALAFSAGAQVKVGVFVPLTGDAAGYGQSALEAIQLAVKQKNDAGGVLGQKIELVVRDDGAKP
EQSVTVAKRLATSDGVLVMLGSISSPASFAATQVALQTGTPQIVLLGTAQRITTQGNPWVFRGTIPDTKLSGDLVEFIIR
KYPGKKKVGYLYVNDDFGRGGFEAFKARAESSGLTIVAEEKYAPGDLDFTSQLTKIQSENPDVFVDWSRYTEGALVAIQA
KRKGMTLPHFGSEGLSHPNFIKLAGDASDGMMYPVPFSMTTQSGNNIAVTLAQDIKKTYNKEIEYTHALAYDAMRAVIFA
IEKSGTTTDKLKFRDALKSLEFDSTRGKFKFDAKGDPTLLPSIVKLSSGKEQDANK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=347, Percent_Identity=23.9193083573487, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789867, Length=354, Percent_Identity=24.2937853107345, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40410; Mature: 40410

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFATRFSKLQACVMFAGVALAFSAGAQVKVGVFVPLTGDAAGYGQSALEAIQLAVKQKND
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
AGGVLGQKIELVVRDDGAKPEQSVTVAKRLATSDGVLVMLGSISSPASFAATQVALQTGT
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHEEEEEECCC
PQIVLLGTAQRITTQGNPWVFRGTIPDTKLSGDLVEFIIRKYPGKKKVGYLYVNDDFGRG
CEEEEEECCHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCC
GFEAFKARAESSGLTIVAEEKYAPGDLDFTSQLTKIQSENPDVFVDWSRYTEGALVAIQA
HHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEE
KRKGMTLPHFGSEGLSHPNFIKLAGDASDGMMYPVPFSMTTQSGNNIAVTLAQDIKKTYN
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEEEECCCCEEEEEEHHHHHHHHC
KEIEYTHALAYDAMRAVIFAIEKSGTTTDKLKFRDALKSLEFDSTRGKFKFDAKGDPTLL
CCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHH
PSIVKLSSGKEQDANK
HHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFATRFSKLQACVMFAGVALAFSAGAQVKVGVFVPLTGDAAGYGQSALEAIQLAVKQKND
CCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCC
AGGVLGQKIELVVRDDGAKPEQSVTVAKRLATSDGVLVMLGSISSPASFAATQVALQTGT
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHEEEEEECCC
PQIVLLGTAQRITTQGNPWVFRGTIPDTKLSGDLVEFIIRKYPGKKKVGYLYVNDDFGRG
CEEEEEECCHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCC
GFEAFKARAESSGLTIVAEEKYAPGDLDFTSQLTKIQSENPDVFVDWSRYTEGALVAIQA
HHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCEEEEEEE
KRKGMTLPHFGSEGLSHPNFIKLAGDASDGMMYPVPFSMTTQSGNNIAVTLAQDIKKTYN
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEEEECCCCEEEEEEHHHHHHHHC
KEIEYTHALAYDAMRAVIFAIEKSGTTTDKLKFRDALKSLEFDSTRGKFKFDAKGDPTLL
CCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHH
PSIVKLSSGKEQDANK
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]