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The map label for this gene is dat [H]

Identifier: 91786228

GI number: 91786228

Start: 341559

End: 342461

Strand: Reverse

Name: dat [H]

Synonym: Bpro_0317

Alternate gene names: 91786228

Gene position: 342461-341559 (Counterclockwise)

Preceding gene: 91786229

Following gene: 91786227

Centisome position: 6.59

GC content: 63.57

Gene sequence:

>903_bases
ATGACGTCCTCTTCCCCCTCTACCCTGCCCGACACACCGTGCTACCTCAACGGCGAGTTCACCAGCCTGAAAGACGCCAA
GATCAGCGTGCTGGACCGGGGCTTCATCTTTGGCGATGGGGTGTATGAGGTCGTGCCGGCCTACGCGGGCAAGCTGTTCC
GCTTTGAGCAGCACATGGCGCGCCTTAGCCGCAGCCTGGCGGAACTGCGCATCGCCAACCCCATGACACACGCCGAGTGG
CGCGAACTCACGCTCAAACTGATAGCGTCTTACGACCAATCCATGCCGGCCGACATGGAGAAAGGCAACCAGCTGATCTA
CATCCAGGTCACCCGCGGCGTGGCCATGCGCGACCACCCCATGCCGCCCGGCCTGACGCCCACCGTCTTCGTCATGACCA
ACGCCCTGAAGTTGCCCGGCGCGCAGCAGCGCGAGCAGGGCGTGGCCTGCGTGACCGCTGACGACTTCCGTTGGGAAAAG
GCCCACATCAAAAGCGTCAGCCTGCTGGGTGCGGTGTTCTCGCGCCAGATCAGCGTGGACGCCGGCGCGGTCGAAACCGT
GATGTTCCGTGACAACTACCTGAGCGAAGCAGCCTCCAGCAACGTCTGGGTCGTCAAGAACGGCACGGTGATGGGACCGC
CGAAGGACCAGCTGGTGCTCGAGGGCATCCGTTACGGCCTGATGGAGGAAATGTGCCGGGCCCTGGGCCTGGGGTTTGAG
CTGCGCCGCATCACGCGCGCCGAAGTACTGGCGGCCGACGAGTTGCTGCTGTCGTCGGCCACCAAGGAGGTACTGCCCGT
CACCCTGCTGGACGGTCAACCGGTTGGAAATGGCAAGCCCGGCCCCATCTATGCCAGCTTGTATGCTGCATACCAGCAGG
CCAAGCGAGAGCAATCAACATGA

Upstream 100 bases:

>100_bases
ATCGATAGACAATCAGCTTCGCCCGCAACACCGCCCCATCCGCCTCCATCGCCTTCACCGCCTCCAATCAGTCCTCCGAC
CTTACTTTTTCCCGTTCATC

Downstream 100 bases:

>100_bases
CGACTTCACCGAAAAACCCGCCGACAACCCCGCCGAACGCCGCGCCCGAACCCGCACCGCGTCAGGAGTCGCTGATCGAC
TACCCTTCGCTCTTCCCCAT

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTSSSPSTLPDTPCYLNGEFTSLKDAKISVLDRGFIFGDGVYEVVPAYAGKLFRFEQHMARLSRSLAELRIANPMTHAEW
RELTLKLIASYDQSMPADMEKGNQLIYIQVTRGVAMRDHPMPPGLTPTVFVMTNALKLPGAQQREQGVACVTADDFRWEK
AHIKSVSLLGAVFSRQISVDAGAVETVMFRDNYLSEAASSNVWVVKNGTVMGPPKDQLVLEGIRYGLMEEMCRALGLGFE
LRRITRAEVLAADELLLSSATKEVLPVTLLDGQPVGNGKPGPIYASLYAAYQQAKREQST

Sequences:

>Translated_300_residues
MTSSSPSTLPDTPCYLNGEFTSLKDAKISVLDRGFIFGDGVYEVVPAYAGKLFRFEQHMARLSRSLAELRIANPMTHAEW
RELTLKLIASYDQSMPADMEKGNQLIYIQVTRGVAMRDHPMPPGLTPTVFVMTNALKLPGAQQREQGVACVTADDFRWEK
AHIKSVSLLGAVFSRQISVDAGAVETVMFRDNYLSEAASSNVWVVKNGTVMGPPKDQLVLEGIRYGLMEEMCRALGLGFE
LRRITRAEVLAADELLLSSATKEVLPVTLLDGQPVGNGKPGPIYASLYAAYQQAKREQST
>Mature_299_residues
TSSSPSTLPDTPCYLNGEFTSLKDAKISVLDRGFIFGDGVYEVVPAYAGKLFRFEQHMARLSRSLAELRIANPMTHAEWR
ELTLKLIASYDQSMPADMEKGNQLIYIQVTRGVAMRDHPMPPGLTPTVFVMTNALKLPGAQQREQGVACVTADDFRWEKA
HIKSVSLLGAVFSRQISVDAGAVETVMFRDNYLSEAASSNVWVVKNGTVMGPPKDQLVLEGIRYGLMEEMCRALGLGFEL
RRITRAEVLAADELLLSSATKEVLPVTLLDGQPVGNGKPGPIYASLYAAYQQAKREQST

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=295, Percent_Identity=26.4406779661017, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32993; Mature: 32862

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSSPSTLPDTPCYLNGEFTSLKDAKISVLDRGFIFGDGVYEVVPAYAGKLFRFEQHMA
CCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHH
RLSRSLAELRIANPMTHAEWRELTLKLIASYDQSMPADMEKGNQLIYIQVTRGVAMRDHP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEEECCCEECCCC
MPPGLTPTVFVMTNALKLPGAQQREQGVACVTADDFRWEKAHIKSVSLLGAVFSRQISVD
CCCCCCCEEEEEEHHHCCCCCCHHHCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHCCC
AGAVETVMFRDNYLSEAASSNVWVVKNGTVMGPPKDQLVLEGIRYGLMEEMCRALGLGFE
CCCEEEEEECCHHHHHHCCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHH
LRRITRAEVLAADELLLSSATKEVLPVTLLDGQPVGNGKPGPIYASLYAAYQQAKREQST
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSSPSTLPDTPCYLNGEFTSLKDAKISVLDRGFIFGDGVYEVVPAYAGKLFRFEQHMA
CCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHH
RLSRSLAELRIANPMTHAEWRELTLKLIASYDQSMPADMEKGNQLIYIQVTRGVAMRDHP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEEEEEECCCEECCCC
MPPGLTPTVFVMTNALKLPGAQQREQGVACVTADDFRWEKAHIKSVSLLGAVFSRQISVD
CCCCCCCEEEEEEHHHCCCCCCHHHCCCEEEEECCCEEHHHHHHHHHHHHHHHHHHHCCC
AGAVETVMFRDNYLSEAASSNVWVVKNGTVMGPPKDQLVLEGIRYGLMEEMCRALGLGFE
CCCEEEEEECCHHHHHHCCCCEEEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHH
LRRITRAEVLAADELLLSSATKEVLPVTLLDGQPVGNGKPGPIYASLYAAYQQAKREQST
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]