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The map label for this gene is rfaF [H]

Identifier: 91786116

GI number: 91786116

Start: 222249

End: 223244

Strand: Direct

Name: rfaF [H]

Synonym: Bpro_0205

Alternate gene names: 91786116

Gene position: 222249-223244 (Clockwise)

Preceding gene: 91786115

Following gene: 91786121

Centisome position: 4.27

GC content: 66.47

Gene sequence:

>996_bases
TTGAGCAAATCACTGATCATTGCCCCGCAGTGGATAGGCGACGCGGTGATGACCGAGCCCTTGCTGCGCCGGCTGCATGC
ACGGGGTGAGCGCATCACGGTCGGGGCGCTGCCCTGGGTGGCGCCGGTGTACCGGGCCATGCCGCAGGTGGCGGAAGTCA
TCGAGTTTCCGTTTGCCCATGGCGGCCTGCAGTTCAAGGCCCGCCGCGCCCTTGCCAAGCGCATCGAAGGCCAGTTTGAC
GCGGCCTACGTGCTGCCCAATTCACTGAAAAGCGCGCTGTTGCCCTTTCTGGCCAGCATTCCGAAACGCGTGGGTTACCT
CGGGGAGGCGCGCGTCGGCCTGCTGACCCATCGCCTGAAAAACCCGAAAAACAAACCGCCGATGGTGGCGTTTTACTCAG
CCCTCAGCGGCGAGCAGGACGTGGCCGGGGACCGGCCGCAGCTGCAGATGGATGCGGCCGACGTGGACCGCGCGCTGGCA
GAGCTCGGACTGGCGCGTGGCGCTTACTACGTGTTTGCGCCGGGCGCCGAATACGGCCCGGCCAAGCGTTGGCCGGCCCG
GCATTTTTCCGAGCTGGCGGCGAAGCTGGACCGGCCGGTCGTGTTGCTCGGCTCCGGCAAGGAGGCGTCGCTGTGCGACG
AGATTGCGGCGCCGGTGAATGCGGCAGAGGCCGGCAAGTGCCTCAACTTTGCCGGCCGGACCTCGCTGCTGCAGGCGCTG
TGCCTGATCGCCGCCAGCAAAAGCACCATCAGCAATGATTCCGGCCTGATGCATGTGGCCGCCGCGCTGGGCGTGCCGCA
GGTGGCCATTTTTGGCTCATCGAGCCCGCTGCACACACCACCGCTGAGCGAGAAGGCCGCCGTGCTCTGGCTGAAAACCG
ACCCGGCCTACCAGCCGCCGCTCGACTGCGCACCTTGCTTTGCGCGCGACTGCCCGCTCGGCCATACCCGCTGCCTGAAT
GACATCACGGCCGATCAGGTGCGCCAAAAGTTATAG

Upstream 100 bases:

>100_bases
CCACTGACGGTTCGTGCCTGGATTGTCATTCCGGGCTTGACCCGGAATCCACCGTTTGCGCGCCAGCATGGATGCCGGAT
CGAGTCCGGCATGACAAACC

Downstream 100 bases:

>100_bases
CGACCAGAGCCCAAAGTTATTGGGCTAGAAGTTGTTTTTGTTCAAAAACTTCAACGGCGTCGGCGCTCATGCGGGCAATC
ACCTCGGCGGCCGGCGCCAG

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSKSLIIAPQWIGDAVMTEPLLRRLHARGERITVGALPWVAPVYRAMPQVAEVIEFPFAHGGLQFKARRALAKRIEGQFD
AAYVLPNSLKSALLPFLASIPKRVGYLGEARVGLLTHRLKNPKNKPPMVAFYSALSGEQDVAGDRPQLQMDAADVDRALA
ELGLARGAYYVFAPGAEYGPAKRWPARHFSELAAKLDRPVVLLGSGKEASLCDEIAAPVNAAEAGKCLNFAGRTSLLQAL
CLIAASKSTISNDSGLMHVAAALGVPQVAIFGSSSPLHTPPLSEKAAVLWLKTDPAYQPPLDCAPCFARDCPLGHTRCLN
DITADQVRQKL

Sequences:

>Translated_331_residues
MSKSLIIAPQWIGDAVMTEPLLRRLHARGERITVGALPWVAPVYRAMPQVAEVIEFPFAHGGLQFKARRALAKRIEGQFD
AAYVLPNSLKSALLPFLASIPKRVGYLGEARVGLLTHRLKNPKNKPPMVAFYSALSGEQDVAGDRPQLQMDAADVDRALA
ELGLARGAYYVFAPGAEYGPAKRWPARHFSELAAKLDRPVVLLGSGKEASLCDEIAAPVNAAEAGKCLNFAGRTSLLQAL
CLIAASKSTISNDSGLMHVAAALGVPQVAIFGSSSPLHTPPLSEKAAVLWLKTDPAYQPPLDCAPCFARDCPLGHTRCLN
DITADQVRQKL
>Mature_330_residues
SKSLIIAPQWIGDAVMTEPLLRRLHARGERITVGALPWVAPVYRAMPQVAEVIEFPFAHGGLQFKARRALAKRIEGQFDA
AYVLPNSLKSALLPFLASIPKRVGYLGEARVGLLTHRLKNPKNKPPMVAFYSALSGEQDVAGDRPQLQMDAADVDRALAE
LGLARGAYYVFAPGAEYGPAKRWPARHFSELAAKLDRPVVLLGSGKEASLCDEIAAPVNAAEAGKCLNFAGRTSLLQALC
LIAASKSTISNDSGLMHVAAALGVPQVAIFGSSSPLHTPPLSEKAAVLWLKTDPAYQPPLDCAPCFARDCPLGHTRCLND
ITADQVRQKL

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=346, Percent_Identity=42.7745664739884, Blast_Score=230, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 35561; Mature: 35429

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLIIAPQWIGDAVMTEPLLRRLHARGERITVGALPWVAPVYRAMPQVAEVIEFPFAH
CCCCEEECHHHHCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
GGLQFKARRALAKRIEGQFDAAYVLPNSLKSALLPFLASIPKRVGYLGEARVGLLTHRLK
CCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
NPKNKPPMVAFYSALSGEQDVAGDRPQLQMDAADVDRALAELGLARGAYYVFAPGAEYGP
CCCCCCCCEEHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECCCCCCCC
AKRWPARHFSELAAKLDRPVVLLGSGKEASLCDEIAAPVNAAEAGKCLNFAGRTSLLQAL
CCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
CLIAASKSTISNDSGLMHVAAALGVPQVAIFGSSSPLHTPPLSEKAAVLWLKTDPAYQPP
HHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCC
LDCAPCFARDCPLGHTRCLNDITADQVRQKL
CCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKSLIIAPQWIGDAVMTEPLLRRLHARGERITVGALPWVAPVYRAMPQVAEVIEFPFAH
CCCEEECHHHHCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
GGLQFKARRALAKRIEGQFDAAYVLPNSLKSALLPFLASIPKRVGYLGEARVGLLTHRLK
CCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
NPKNKPPMVAFYSALSGEQDVAGDRPQLQMDAADVDRALAELGLARGAYYVFAPGAEYGP
CCCCCCCCEEHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCEEEECCCCCCCC
AKRWPARHFSELAAKLDRPVVLLGSGKEASLCDEIAAPVNAAEAGKCLNFAGRTSLLQAL
CCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
CLIAASKSTISNDSGLMHVAAALGVPQVAIFGSSSPLHTPPLSEKAAVLWLKTDPAYQPP
HHHHHCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCC
LDCAPCFARDCPLGHTRCLNDITADQVRQKL
CCCCHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]