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The map label for this gene is radA [H]

Identifier: 91786111

GI number: 91786111

Start: 217927

End: 219324

Strand: Direct

Name: radA [H]

Synonym: Bpro_0200

Alternate gene names: 91786111

Gene position: 217927-219324 (Clockwise)

Preceding gene: 91786105

Following gene: 91786112

Centisome position: 4.19

GC content: 66.52

Gene sequence:

>1398_bases
ATGGCCAAAGAAAAAACGATTTACACCTGCACCGAATGCGGCGGCACCAGCCCCAAGTGGCTGGGCAAGTGCCCGCACTG
CACTGCCTGGAACACGCTGATCGAGTCGGTCGCCGAAAACAGCTCACCTGTCAAAAACCGTTTTGCATCCCTGGCCAAGG
CTTCCGAGGTGACCACCCTCTCCGACATTGAGGCCACCGACATGGCGCGCACACCCACGGGCCATGAGGAGCTGGACCGC
GTGCTGGGCGGCGGCATTGTGGACGGCGGGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGCT
GCAGGCGCTGGACTCCCTGCAACGCGCCGGCCTGAACACGCTGTATGTGACGGGCGAGGAAAGCGGGGCGCAGGTTGCGC
TGCGCTCGCGCCGGCTGGGGCTGGACGGCTCGCAGGTCAAGGTGCTGGCGGAGATTCAGCTGGAGAAAATCCTCGCCACG
CTGGAGGCGACCCGGCCGGCGATTGCCGTCATTGACTCGATCCAGACGGTGTACTCCGACCAGCTCACCTCGGCGCCCGG
TTCGGTGGCGCAGGTGCGCGAATGCGCGGCCCACCTGACGCGCTACGCCAAAGCCAGCGGACAATCTGACGGTGTTGCCC
GTGGCCGGGCAACGTGCATCGTGCTGGTCGGCCATGTCACCAAAGAGGGCGCGTTGGCCGGCCCGCGCGTGCTGGAGCAC
ATGGTCGACACCGTGCTGTACTTCGAAGGCGACACGCATTCGAGCTTTCGCCTGGTGCGCGCCATCAAGAACCGCTTCGG
CGCCGTCAATGAAATCGGCGTGTTTGCCATGACGGAGAAGGGCCTCAAGGGCGTGACCAACCCGAGCGCGATTTTCCTGT
CCCAGCACAGTGAGCCGGTGCCCGGCAGCTGCGTGCTGGTCACGCTGGAAGGCACACGCCCGATGCTGGTCGAGATCCAG
GCGCTGGTCGACAACGGCGGCCCCAGCCCGCGCCGACTCTCGGTTGGCCTGGACCGTGACCGGCTGGCCATGCTGCTGGC
CGTGCTGCACCGCCATGCCGGCGTGGCCTGCATGGACCAGGACGTGTTCGTCAATGCGGTAGGCGGCGTGCGCATCAGCG
AGCCGGCCGCCGACCTGAGCGTGATGCTGGCCATCACCTCCAGCCTGCGCGGCAAACCGCTGCCCAAGGGCTTCATTGCT
TTTGGCGAGGTGGGACTGGCCGGCGAGGTGCGCCCCGCGCCGCGCGGGCAGGAGCGCCTGAAGGAAGCGGCCAAGCTGGG
GTTTAGCGTGGCGGTGGTGCCCAAGGCCAATGCGCCGAGAAAGCCGATTGAAGGGCTGATCATCCATGCCGTGGAGCGGG
TGGAGCAGGCCATGGAAGTGGTGCGCAGTTTGAATTGA

Upstream 100 bases:

>100_bases
AGCCGGCCAGTCAGCGATTATTCACCTGCCCGCAAAAACAGGTGGCGTGCCAACATTGACAAAGCAGCAATACTGGTTGT
TTGTACAGTAATATCCGTTC

Downstream 100 bases:

>100_bases
GCGCAGTTTGGTTCTAGAGTCATGCATGTAGCCAGTTCGTTTTCAAGCCCTCTCGAACCACCTCGGCCATCTCATGAGAA
TTGCAGTCATTTCGGACATC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAKEKTIYTCTECGGTSPKWLGKCPHCTAWNTLIESVAENSSPVKNRFASLAKASEVTTLSDIEATDMARTPTGHEELDR
VLGGGIVDGGVVLIGGDPGIGKSTLLLQALDSLQRAGLNTLYVTGEESGAQVALRSRRLGLDGSQVKVLAEIQLEKILAT
LEATRPAIAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRYAKASGQSDGVARGRATCIVLVGHVTKEGALAGPRVLEH
MVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQ
ALVDNGGPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLSVMLAITSSLRGKPLPKGFIA
FGEVGLAGEVRPAPRGQERLKEAAKLGFSVAVVPKANAPRKPIEGLIIHAVERVEQAMEVVRSLN

Sequences:

>Translated_465_residues
MAKEKTIYTCTECGGTSPKWLGKCPHCTAWNTLIESVAENSSPVKNRFASLAKASEVTTLSDIEATDMARTPTGHEELDR
VLGGGIVDGGVVLIGGDPGIGKSTLLLQALDSLQRAGLNTLYVTGEESGAQVALRSRRLGLDGSQVKVLAEIQLEKILAT
LEATRPAIAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRYAKASGQSDGVARGRATCIVLVGHVTKEGALAGPRVLEH
MVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQ
ALVDNGGPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLSVMLAITSSLRGKPLPKGFIA
FGEVGLAGEVRPAPRGQERLKEAAKLGFSVAVVPKANAPRKPIEGLIIHAVERVEQAMEVVRSLN
>Mature_464_residues
AKEKTIYTCTECGGTSPKWLGKCPHCTAWNTLIESVAENSSPVKNRFASLAKASEVTTLSDIEATDMARTPTGHEELDRV
LGGGIVDGGVVLIGGDPGIGKSTLLLQALDSLQRAGLNTLYVTGEESGAQVALRSRRLGLDGSQVKVLAEIQLEKILATL
EATRPAIAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRYAKASGQSDGVARGRATCIVLVGHVTKEGALAGPRVLEHM
VDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQA
LVDNGGPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLSVMLAITSSLRGKPLPKGFIAF
GEVGLAGEVRPAPRGQERLKEAAKLGFSVAVVPKANAPRKPIEGLIIHAVERVEQAMEVVRSLN

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=472, Percent_Identity=53.6016949152542, Blast_Score=485, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49153; Mature: 49022

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKTIYTCTECGGTSPKWLGKCPHCTAWNTLIESVAENSSPVKNRFASLAKASEVTTL
CCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
SDIEATDMARTPTGHEELDRVLGGGIVDGGVVLIGGDPGIGKSTLLLQALDSLQRAGLNT
CCCCHHHCCCCCCCHHHHHHHHCCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCE
LYVTGEESGAQVALRSRRLGLDGSQVKVLAEIQLEKILATLEATRPAIAVIDSIQTVYSD
EEEECCCCCCCEEEHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QLTSAPGSVAQVRECAAHLTRYAKASGQSDGVARGRATCIVLVGHVTKEGALAGPRVLEH
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
MVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPV
HHHHEEEECCCCCHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCCCCEEEEECCCCCC
PGSCVLVTLEGTRPMLVEIQALVDNGGPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQ
CCCEEEEEECCCCCEEEEEEEHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECC
DVFVNAVGGVRISEPAADLSVMLAITSSLRGKPLPKGFIAFGEVGLAGEVRPAPRGQERL
CEEEEEECCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHH
KEAAKLGFSVAVVPKANAPRKPIEGLIIHAVERVEQAMEVVRSLN
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKEKTIYTCTECGGTSPKWLGKCPHCTAWNTLIESVAENSSPVKNRFASLAKASEVTTL
CCCCEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
SDIEATDMARTPTGHEELDRVLGGGIVDGGVVLIGGDPGIGKSTLLLQALDSLQRAGLNT
CCCCHHHCCCCCCCHHHHHHHHCCCEECCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCE
LYVTGEESGAQVALRSRRLGLDGSQVKVLAEIQLEKILATLEATRPAIAVIDSIQTVYSD
EEEECCCCCCCEEEHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QLTSAPGSVAQVRECAAHLTRYAKASGQSDGVARGRATCIVLVGHVTKEGALAGPRVLEH
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
MVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVTNPSAIFLSQHSEPV
HHHHEEEECCCCCHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCCCCEEEEECCCCCC
PGSCVLVTLEGTRPMLVEIQALVDNGGPSPRRLSVGLDRDRLAMLLAVLHRHAGVACMDQ
CCCEEEEEECCCCCEEEEEEEHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEEECC
DVFVNAVGGVRISEPAADLSVMLAITSSLRGKPLPKGFIAFGEVGLAGEVRPAPRGQERL
CEEEEEECCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHH
KEAAKLGFSVAVVPKANAPRKPIEGLIIHAVERVEQAMEVVRSLN
HHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]