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The map label for this gene is gshA [H]

Identifier: 91785794

GI number: 91785794

Start: 4871281

End: 4872897

Strand: Reverse

Name: gshA [H]

Synonym: Bxe_A4484

Alternate gene names: 91785794

Gene position: 4872897-4871281 (Counterclockwise)

Preceding gene: 91785795

Following gene: 91785793

Centisome position: 99.53

GC content: 62.59

Gene sequence:

>1617_bases
ATGCCAAACACGACATCTTCCCGCACGACCGACGCGTTCTCGCACCGCCTGTCCGTGCTGACCACAGGCCCGCAGCGCGA
TGCGCTGATTCGTGGCCTGCGCGGCATCGAAAAGGAAAGCCTGCGCGTGACGCACGAGGGCAAGCTCGCCTTGACGCCGC
ATCCACGCGCGCTCGGTTCGGCTCTCACGCATCCGTCGCTAACCACGGACTATTCCGAAGCGCTGCTCGAATTGATCACG
CCGGCCGAGCACGACGTCGCGCTGACGCTCGAGAAGCTCGACACGCTGCATCGTTTTGTCTATGCGGAACTCGGCGACGA
GATTCTGTGGAACAACTCCATGCCCGGCCTGCTGCCCGGTACCGACGAGGGTATTCCGATCGCGCACTACGGCAGTTCGA
ATATCGGCAAACTGAAGTACGTCTACCGCATTGGTCTCGCGCTCCGTTACGGACGCACCATGCAGTGCATTGCCGGCATC
CACTACAACTATTCGCTGAACGAAGAAGTATGGCGGCGGCTGCATGCCGATCAGCAATCCACCGCGAACGCCGTCGACTT
CCAGTCCGACCGCTACCTCGCGCTGATCCGCAATTTCCGCCGCACCAACTGGCTGTTGATGTATCTGTTCGGTGCTTCGC
CGGCGCTGGATCGCCGTTTCTTGCGCGAGCGCAAACATTCGCTCGATACGTTCGACGCCGACACGCTCTACCGGCCGTAC
GCGACCAGCCTGCGCATGAGCGACCTCGGCTATTCGAACACCACGGCGCAAGCCGCGCTGCACGCGGATTACGACACGCT
GCCAGGTTATCTCGACGCGCTGGCGAAAGCCGTGAGCCAGCCATACCCCGCGTACGAGGCAATCGGCACACAGCGCGATG
GCGAGTGGGTACAGATCAACACGAACGTGCTGCAGATCGAAAACGAGTTTTACTCGACGATCCGCCCGAAGCGCATTACG
TATCCGGGCGAGCGTCCGCTGCATGCACTGGCAGCGCGCGGCGTGCAATACGTGGAAGTGCGCTGCATGGATATCGATCC
GTTCGAACCGACCGGCATTTCGCTTGAAACGTCGCGCTTTCTCGACGCCTATCTGCTGGTCTGCGCGCTCGACGACAGCG
CGCTGCTGCCGCCTGATGCCTATGCCGAAGCCAATCACAACTTCGCCCGCGTGACGATGGAAGGGCGCAAGCCCGGCCTC
GAACTGACACGCGACGGCCAGCCGGTCGCGATGGCGGATTGGGCGAACGAACTGCTCGTCAAGATCGACGCGGCTGCGGC
CACGCTCGACGCTTTGCACGGCGGCGACGCGCATGCACGCGCTGTCGCCGTTCAACGCGCGAAGCTGGCCGATGTATCGC
TGACACCGTCGGCGCGCGTGCTGCAGACCATGCGCGAGAAGCAGCAGAGTTTCCTCGCCTTCGGCCTTGCGCAAAGCGAA
GCGCACGCCGCGTATTTTCGCGCGCGTCCGCTGGACGCGGAGCAGACCAAGGTATTCACCGATCTCGCTGCACAGTCGCT
GGCTGAACAGGCGAAACTCGAACGGGAAGAAGTCGGTTCGTTCGACGCATTCGTCGCGGCTTATCGCGCGTACACGTTGA
ACCGGTTCAGCGTCTGA

Upstream 100 bases:

>100_bases
GGCGAGATGTTAAAGTGCCCGCTACTGAAAATTCCGCCGAACGTTGTCAACCGCGCTGTGCTGCGGCACATGGCGGCCCG
ACAACGCCCGACTCGCATCA

Downstream 100 bases:

>100_bases
ACAACGCGCGCGTGGGTAAGGCGTGGGTGAAGTGAGAAGAGAAACAGGCGGTCGGAAAGGCCGCTTTTTTCTGCGCAGGT
AACGTCTACGCAATGCGCCA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MPNTTSSRTTDAFSHRLSVLTTGPQRDALIRGLRGIEKESLRVTHEGKLALTPHPRALGSALTHPSLTTDYSEALLELIT
PAEHDVALTLEKLDTLHRFVYAELGDEILWNNSMPGLLPGTDEGIPIAHYGSSNIGKLKYVYRIGLALRYGRTMQCIAGI
HYNYSLNEEVWRRLHADQQSTANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRERKHSLDTFDADTLYRPY
ATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPAYEAIGTQRDGEWVQINTNVLQIENEFYSTIRPKRIT
YPGERPLHALAARGVQYVEVRCMDIDPFEPTGISLETSRFLDAYLLVCALDDSALLPPDAYAEANHNFARVTMEGRKPGL
ELTRDGQPVAMADWANELLVKIDAAAATLDALHGGDAHARAVAVQRAKLADVSLTPSARVLQTMREKQQSFLAFGLAQSE
AHAAYFRARPLDAEQTKVFTDLAAQSLAEQAKLEREEVGSFDAFVAAYRAYTLNRFSV

Sequences:

>Translated_538_residues
MPNTTSSRTTDAFSHRLSVLTTGPQRDALIRGLRGIEKESLRVTHEGKLALTPHPRALGSALTHPSLTTDYSEALLELIT
PAEHDVALTLEKLDTLHRFVYAELGDEILWNNSMPGLLPGTDEGIPIAHYGSSNIGKLKYVYRIGLALRYGRTMQCIAGI
HYNYSLNEEVWRRLHADQQSTANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRERKHSLDTFDADTLYRPY
ATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPAYEAIGTQRDGEWVQINTNVLQIENEFYSTIRPKRIT
YPGERPLHALAARGVQYVEVRCMDIDPFEPTGISLETSRFLDAYLLVCALDDSALLPPDAYAEANHNFARVTMEGRKPGL
ELTRDGQPVAMADWANELLVKIDAAAATLDALHGGDAHARAVAVQRAKLADVSLTPSARVLQTMREKQQSFLAFGLAQSE
AHAAYFRARPLDAEQTKVFTDLAAQSLAEQAKLEREEVGSFDAFVAAYRAYTLNRFSV
>Mature_537_residues
PNTTSSRTTDAFSHRLSVLTTGPQRDALIRGLRGIEKESLRVTHEGKLALTPHPRALGSALTHPSLTTDYSEALLELITP
AEHDVALTLEKLDTLHRFVYAELGDEILWNNSMPGLLPGTDEGIPIAHYGSSNIGKLKYVYRIGLALRYGRTMQCIAGIH
YNYSLNEEVWRRLHADQQSTANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRERKHSLDTFDADTLYRPYA
TSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPAYEAIGTQRDGEWVQINTNVLQIENEFYSTIRPKRITY
PGERPLHALAARGVQYVEVRCMDIDPFEPTGISLETSRFLDAYLLVCALDDSALLPPDAYAEANHNFARVTMEGRKPGLE
LTRDGQPVAMADWANELLVKIDAAAATLDALHGGDAHARAVAVQRAKLADVSLTPSARVLQTMREKQQSFLAFGLAQSEA
HAAYFRARPLDAEQTKVFTDLAAQSLAEQAKLEREEVGSFDAFVAAYRAYTLNRFSV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=517, Percent_Identity=39.8452611218569, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60002; Mature: 59871

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNTTSSRTTDAFSHRLSVLTTGPQRDALIRGLRGIEKESLRVTHEGKLALTPHPRALGS
CCCCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALLELITPAEHDVALTLEKLDTLHRFVYAELGDEILWNNSMPGLLPG
HHCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
TDEGIPIAHYGSSNIGKLKYVYRIGLALRYGRTMQCIAGIHYNYSLNEEVWRRLHADQQS
CCCCCEEEECCCCCCCCEEHHHHHHHHHHCCCCEEEHHCEECCCCCCHHHHHHHCCCCHH
TANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRERKHSLDTFDADTLYRPY
CCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCH
ATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPAYEAIGTQRDGEWVQIN
HHCEEHHCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEE
TNVLQIENEFYSTIRPKRITYPGERPLHALAARGVQYVEVRCMDIDPFEPTGISLETSRF
CCEEEECHHHHHHCCCCEECCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEHHHH
LDAYLLVCALDDSALLPPDAYAEANHNFARVTMEGRKPGLELTRDGQPVAMADWANELLV
HHHHEEEEEECCCCCCCCCHHHCCCCCEEEEEECCCCCCCCEECCCCCEEHHHHHHHHHE
KIDAAAATLDALHGGDAHARAVAVQRAKLADVSLTPSARVLQTMREKQQSFLAFGLAQSE
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AHAAYFRARPLDAEQTKVFTDLAAQSLAEQAKLEREEVGSFDAFVAAYRAYTLNRFSV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNTTSSRTTDAFSHRLSVLTTGPQRDALIRGLRGIEKESLRVTHEGKLALTPHPRALGS
CCCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHCCCCCCEEEEECCEEEECCCCHHHHH
ALTHPSLTTDYSEALLELITPAEHDVALTLEKLDTLHRFVYAELGDEILWNNSMPGLLPG
HHCCCCCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
TDEGIPIAHYGSSNIGKLKYVYRIGLALRYGRTMQCIAGIHYNYSLNEEVWRRLHADQQS
CCCCCEEEECCCCCCCCEEHHHHHHHHHHCCCCEEEHHCEECCCCCCHHHHHHHCCCCHH
TANAVDFQSDRYLALIRNFRRTNWLLMYLFGASPALDRRFLRERKHSLDTFDADTLYRPY
CCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCH
ATSLRMSDLGYSNTTAQAALHADYDTLPGYLDALAKAVSQPYPAYEAIGTQRDGEWVQIN
HHCEEHHCCCCCCCHHHHEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEE
TNVLQIENEFYSTIRPKRITYPGERPLHALAARGVQYVEVRCMDIDPFEPTGISLETSRF
CCEEEECHHHHHHCCCCEECCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCEEHHHH
LDAYLLVCALDDSALLPPDAYAEANHNFARVTMEGRKPGLELTRDGQPVAMADWANELLV
HHHHEEEEEECCCCCCCCCHHHCCCCCEEEEEECCCCCCCCEECCCCCEEHHHHHHHHHE
KIDAAAATLDALHGGDAHARAVAVQRAKLADVSLTPSARVLQTMREKQQSFLAFGLAQSE
EEHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AHAAYFRARPLDAEQTKVFTDLAAQSLAEQAKLEREEVGSFDAFVAAYRAYTLNRFSV
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA