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The map label for this gene is alkJ [H]

Identifier: 91785757

GI number: 91785757

Start: 4830181

End: 4831896

Strand: Reverse

Name: alkJ [H]

Synonym: Bxe_A0016

Alternate gene names: 91785757

Gene position: 4831896-4830181 (Counterclockwise)

Preceding gene: 91785758

Following gene: 91785756

Centisome position: 98.69

GC content: 62.94

Gene sequence:

>1716_bases
ATGAGCTATACCGACACCACGCCCGAGACTGCCCGGCGGCTCGAAGGCGAGTTCGACTACATCATCGTGGGCGCGGGGAC
GGCGGGATGTGTGCTGGCCAATCGCCTGAGCGAAGATCCGGACGTGCAGGTGTTGCTGCTCGAAGCCGGCGGCAAGGACG
ATTACCACTGGATTCACGTGCCGGTCGGCTACCTCTATTGCATCGGCAATCCGCGCACGGACTGGCTTTACAAAACCCAG
GCCGAAGCCGGGCTCAATGGCCGCGCGCTTTCGTATCCACGCGGACGCGTATTGGGCGGCAGTTCGTCCATCAACGGCAT
GATCTACATGCGTGGTCAGCGCGAAGACTACGATGAATGGGCGCGAGTCACCAACGACTCATCCTGGTCCTGGAACTCAG
TGCTGCCCGTCTTCAGGCGTAGCGAAGATCATTACGCCGGCGCGAGCGAATCTCATGGCGCGGGCGGCCCTTGGCGCGTC
GAGAAACAGCGGTTGAAATGGAAAATTCTCGAAGAGTTTTCGCGTGCGGCGCAAGAGGCCGGCATTCCCGCAACCGACGA
CTTCAACCGTGGCGACAACACCGGCGTCGGCTACTTCGACGTCAATCAGAAACGCGGCATTCGCTGGAACGCGTCGAAGG
CATTCTTGCGTCCCGCACTCAAGCGTCCGAATCTCACGGTCATCACCGGCGCGCACACGCAGCGCGTCGTCTTCGAAGGA
CGCCGTTGCACGGGCGTCGAATATCGCGGCAACGACACGGACTATCTGGCCAAGGCTCGCTGCGAAGTGATCCTGAGCTC
GGGGGCCGTCAACTCGCCGCAATTGCTCGAATTGTCCGGCATCGGCAATGGCGCGCGTCTGCAGAACCTGGGCATCGAGG
TCGTCAACGATCTGCGTGGCGTCGGAGAGAATCTACAAGACCATTTGCAATTGCGGATGGCTTATAAGGTCGACGGCGTA
CGCACGCTCAACACCGCGTCCGCGCATTGGTGGGGCAAGCTGATGATCGGCCTGCAGTACGCGCTGTTCCAAAGCGGCCC
CATGTCGATGTCGCCGTCGCAACTTGGTGCGTTTGCCAGGTCCGATCCGAACGATCGCTCGCTCACGCGGCCCGACCTCG
AATATCACGTCCAGCCGCTCTCGCTCGATCGCTTCGGCGAACCGCTGCATCGGTTCAATGCGTTCACCGCGTCGGTGTGC
CAGTTGCGGCCCACTTCGCGCGGCAGCATTCACATCGAATCGGCCAATGCTTCAGCGCCGCCGTTGATCGCGCCGAACTA
TCTCTCCACCGATTACGACCGGCACGTCGCCGCCAACGCACTGCGTCTCACGCGGCGCATTGCCGCGGCGCCGGCGCTTG
CACGCTATCGTCCGCAAGAGATTTTGCCCGGCACTCAGTACCAGACGGAAGAGGAGTTGCAGCAGGCTGCCGGCGCGGTC
GGCACGACCATCTTCCATCCGGTCGGCACCTGCCGCATGGGCACGACCGACGACCCCGGCGCGGTGGTCGACAACCGGCT
ACGGGTTATCGGTGTTGACGGACTGCGCGTGGTCGATGCATCCGTCATGCCGACCATCACCTCGGGCAATACCAATTCGC
CCACGCTGATGATCGCGGAGCGCGCCAGCGACATGATCCGCGAGGATCGCCGTGCGCGCGTGGCCGCCAGTTCCGTGGCG
CAAACCAGCCCGGCGCCTTCAGCATTCGCAGTGTGA

Upstream 100 bases:

>100_bases
AACGCGCGCAAAGAAGGCAGAAAACACAATCAAAGCCCAGCGCCGTGGCTGCATGGCGACCATGTAACCCATCGGCGAAA
AAAGCTTAGGAGACTGAACA

Downstream 100 bases:

>100_bases
CAGCTGACATGCGACTCGCATGAGATACTCGCCCGCCCTGCGCGGCGGGCATCTCGTGCGCGCGGCCGCGTTCGGGACGG
GCATCATCCACTAAGTGCAA

Product: GMC family oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSYTDTTPETARRLEGEFDYIIVGAGTAGCVLANRLSEDPDVQVLLLEAGGKDDYHWIHVPVGYLYCIGNPRTDWLYKTQ
AEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQREDYDEWARVTNDSSWSWNSVLPVFRRSEDHYAGASESHGAGGPWRV
EKQRLKWKILEEFSRAAQEAGIPATDDFNRGDNTGVGYFDVNQKRGIRWNASKAFLRPALKRPNLTVITGAHTQRVVFEG
RRCTGVEYRGNDTDYLAKARCEVILSSGAVNSPQLLELSGIGNGARLQNLGIEVVNDLRGVGENLQDHLQLRMAYKVDGV
RTLNTASAHWWGKLMIGLQYALFQSGPMSMSPSQLGAFARSDPNDRSLTRPDLEYHVQPLSLDRFGEPLHRFNAFTASVC
QLRPTSRGSIHIESANASAPPLIAPNYLSTDYDRHVAANALRLTRRIAAAPALARYRPQEILPGTQYQTEEELQQAAGAV
GTTIFHPVGTCRMGTTDDPGAVVDNRLRVIGVDGLRVVDASVMPTITSGNTNSPTLMIAERASDMIREDRRARVAASSVA
QTSPAPSAFAV

Sequences:

>Translated_571_residues
MSYTDTTPETARRLEGEFDYIIVGAGTAGCVLANRLSEDPDVQVLLLEAGGKDDYHWIHVPVGYLYCIGNPRTDWLYKTQ
AEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQREDYDEWARVTNDSSWSWNSVLPVFRRSEDHYAGASESHGAGGPWRV
EKQRLKWKILEEFSRAAQEAGIPATDDFNRGDNTGVGYFDVNQKRGIRWNASKAFLRPALKRPNLTVITGAHTQRVVFEG
RRCTGVEYRGNDTDYLAKARCEVILSSGAVNSPQLLELSGIGNGARLQNLGIEVVNDLRGVGENLQDHLQLRMAYKVDGV
RTLNTASAHWWGKLMIGLQYALFQSGPMSMSPSQLGAFARSDPNDRSLTRPDLEYHVQPLSLDRFGEPLHRFNAFTASVC
QLRPTSRGSIHIESANASAPPLIAPNYLSTDYDRHVAANALRLTRRIAAAPALARYRPQEILPGTQYQTEEELQQAAGAV
GTTIFHPVGTCRMGTTDDPGAVVDNRLRVIGVDGLRVVDASVMPTITSGNTNSPTLMIAERASDMIREDRRARVAASSVA
QTSPAPSAFAV
>Mature_570_residues
SYTDTTPETARRLEGEFDYIIVGAGTAGCVLANRLSEDPDVQVLLLEAGGKDDYHWIHVPVGYLYCIGNPRTDWLYKTQA
EAGLNGRALSYPRGRVLGGSSSINGMIYMRGQREDYDEWARVTNDSSWSWNSVLPVFRRSEDHYAGASESHGAGGPWRVE
KQRLKWKILEEFSRAAQEAGIPATDDFNRGDNTGVGYFDVNQKRGIRWNASKAFLRPALKRPNLTVITGAHTQRVVFEGR
RCTGVEYRGNDTDYLAKARCEVILSSGAVNSPQLLELSGIGNGARLQNLGIEVVNDLRGVGENLQDHLQLRMAYKVDGVR
TLNTASAHWWGKLMIGLQYALFQSGPMSMSPSQLGAFARSDPNDRSLTRPDLEYHVQPLSLDRFGEPLHRFNAFTASVCQ
LRPTSRGSIHIESANASAPPLIAPNYLSTDYDRHVAANALRLTRRIAAAPALARYRPQEILPGTQYQTEEELQQAAGAVG
TTIFHPVGTCRMGTTDDPGAVVDNRLRVIGVDGLRVVDASVMPTITSGNTNSPTLMIAERASDMIREDRRARVAASSVAQ
TSPAPSAFAV

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=43.6802973977695, Blast_Score=425, Evalue=1e-119,
Organism=Escherichia coli, GI1786503, Length=538, Percent_Identity=39.5910780669145, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17532301, Length=559, Percent_Identity=35.2415026833631, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24642055, Length=578, Percent_Identity=36.8512110726644, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24642042, Length=581, Percent_Identity=36.144578313253, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI45549471, Length=590, Percent_Identity=36.271186440678, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI45551458, Length=590, Percent_Identity=36.271186440678, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24642059, Length=570, Percent_Identity=36.8421052631579, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI24642048, Length=585, Percent_Identity=35.2136752136752, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24650267, Length=588, Percent_Identity=34.5238095238095, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859995, Length=570, Percent_Identity=35.2631578947368, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI17137792, Length=567, Percent_Identity=34.5679012345679, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24642035, Length=586, Percent_Identity=33.9590443686007, Blast_Score=277, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24642039, Length=586, Percent_Identity=34.9829351535836, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI18859993, Length=584, Percent_Identity=31.8493150684932, Blast_Score=265, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24642037, Length=574, Percent_Identity=32.5783972125435, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24642051, Length=583, Percent_Identity=30.5317324185249, Blast_Score=254, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642057, Length=596, Percent_Identity=31.2080536912752, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=29.2096219931272, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 62696; Mature: 62565

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTDTTPETARRLEGEFDYIIVGAGTAGCVLANRLSEDPDVQVLLLEAGGKDDYHWIHV
CCCCCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEE
PVGYLYCIGNPRTDWLYKTQAEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQREDYDEW
ECEEEEECCCCCCCCEEEECCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCHHHH
ARVTNDSSWSWNSVLPVFRRSEDHYAGASESHGAGGPWRVEKQRLKWKILEEFSRAAQEA
HHHCCCCCCCHHCCCHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHC
GIPATDDFNRGDNTGVGYFDVNQKRGIRWNASKAFLRPALKRPNLTVITGAHTQRVVFEG
CCCCCCCCCCCCCCCEEEEECCCCCCCEECCHHHHHHHHHCCCCEEEEECCCCEEEEECC
RRCTGVEYRGNDTDYLAKARCEVILSSGAVNSPQLLELSGIGNGARLQNLGIEVVNDLRG
CCEECEEECCCCHHHHHHHHEEEEEECCCCCCCCEEEEECCCCCCEEHHCCHHHHHHHHH
VGENLQDHLQLRMAYKVDGVRTLNTASAHWWGKLMIGLQYALFQSGPMSMSPSQLGAFAR
HCCCHHHHHHEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
SDPNDRSLTRPDLEYHVQPLSLDRFGEPLHRFNAFTASVCQLRPTSRGSIHIESANASAP
CCCCCCCCCCCCHHEEEEECCHHHHCCHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCC
PLIAPNYLSTDYDRHVAANALRLTRRIAAAPALARYRPQEILPGTQYQTEEELQQAAGAV
CEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCHHCCCCCCCCCHHHHHHHHHHH
GTTIFHPVGTCRMGTTDDPGAVVDNRLRVIGVDGLRVVDASVMPTITSGNTNSPTLMIAE
HHHHCCCCCCCCCCCCCCCCCHHCCCEEEEECCCCEEECCCCCCEEECCCCCCCEEEEEH
RASDMIREDRRARVAASSVAQTSPAPSAFAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SYTDTTPETARRLEGEFDYIIVGAGTAGCVLANRLSEDPDVQVLLLEAGGKDDYHWIHV
CCCCCCHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEE
PVGYLYCIGNPRTDWLYKTQAEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQREDYDEW
ECEEEEECCCCCCCCEEEECCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCHHHH
ARVTNDSSWSWNSVLPVFRRSEDHYAGASESHGAGGPWRVEKQRLKWKILEEFSRAAQEA
HHHCCCCCCCHHCCCHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHC
GIPATDDFNRGDNTGVGYFDVNQKRGIRWNASKAFLRPALKRPNLTVITGAHTQRVVFEG
CCCCCCCCCCCCCCCEEEEECCCCCCCEECCHHHHHHHHHCCCCEEEEECCCCEEEEECC
RRCTGVEYRGNDTDYLAKARCEVILSSGAVNSPQLLELSGIGNGARLQNLGIEVVNDLRG
CCEECEEECCCCHHHHHHHHEEEEEECCCCCCCCEEEEECCCCCCEEHHCCHHHHHHHHH
VGENLQDHLQLRMAYKVDGVRTLNTASAHWWGKLMIGLQYALFQSGPMSMSPSQLGAFAR
HCCCHHHHHHEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHC
SDPNDRSLTRPDLEYHVQPLSLDRFGEPLHRFNAFTASVCQLRPTSRGSIHIESANASAP
CCCCCCCCCCCCHHEEEEECCHHHHCCHHHHHHHHHHHHHEECCCCCCEEEEECCCCCCC
PLIAPNYLSTDYDRHVAANALRLTRRIAAAPALARYRPQEILPGTQYQTEEELQQAAGAV
CEECCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHCCCHHCCCCCCCCCHHHHHHHHHHH
GTTIFHPVGTCRMGTTDDPGAVVDNRLRVIGVDGLRVVDASVMPTITSGNTNSPTLMIAE
HHHHCCCCCCCCCCCCCCCCCHHCCCEEEEECCCCEEECCCCCCEEECCCCCCCEEEEEH
RASDMIREDRRARVAASSVAQTSPAPSAFAV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]