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The map label for this gene is flgJ [H]

Identifier: 91785629

GI number: 91785629

Start: 4687781

End: 4688779

Strand: Reverse

Name: flgJ [H]

Synonym: Bxe_A0148

Alternate gene names: 91785629

Gene position: 4688779-4687781 (Counterclockwise)

Preceding gene: 91785630

Following gene: 91785628

Centisome position: 95.77

GC content: 63.96

Gene sequence:

>999_bases
ATGAATTCGGATACGACCAATTCCGCCGCTTCGGCGAACGACCTGACCCAGCGCTTCGCGCTCGACGTGCAGGGCTTCGC
GAAGCTGAGCGCGCAGGCCAAGGCATCCCCGCAAGCCGGCATGAAGATGGCCGCGCAACAGTTCGACGCGGTATTCACGC
AGATGATGCTGAAGAGCATGCGCGACGCGACCCCGCAGGACGGTCCGTTCGATTCGCACGACAGCGCCACCTTCACGTCG
ATGATGGATCAGCAGTTGTCGCAGCAGCTTTCGCAAAAGGGGATTGGCGTGGCGGACGCCATGCTCAAGCAGTTGATGCG
CAATCAGGGCATGCAGGTGGGCGGCGGTGCGGGCGCCGCGGGTGGATTGGCCGGCATGGCCAACGCGTTGGGCGGCGGCA
GCGGCGGCGACGAAGGCCAGACGGCGGCGCTGAACGCGCTCGCGAGAGCGTACGGCAATGCCCAGGCCAACGGCCAGTTG
GCGATCGGCAGGGGCTATTCGGCCAACAGCGCGCTCACTCCGCCGCTCAAGGGCGACGGCAGCTCACCGAAGGTCGACGC
TTTCGTGGACAAGCTCGCCGGGCCGGCCCAGGCCGCGAGCGCGGCGACCGGCATACCGGCGCGCTTCATCATCGGTCAGG
CCGCGCTCGAATCGGGCTGGGGCAAGAGCGAGATCCGGAAGACGGACGGTTCGACCAGCCACAATGTGTTCGGCATCAAG
GCGACCAAAGACTGGACCGGCAAGACTGTGTCGACGGTCACGACTGAATATGTGAACGGCAAGCCGCAGCGCACCGTCGA
AAAATTCCGTGCGTACGACTCGTATCAGGAAGCCATGACCGACTATGCGAGCCTGCTCAAGGGCAATCCGCGCTATGCGC
AGGTGATCAATTCGGCACACGACGTGAACGGTTTTGCCAACGGCATGCAGCGCGCCGGCTACGCCACCGATCCGCATTAC
GCGAAAAAACTGATGTCCATCATGCAGAAGATGGGCTGA

Upstream 100 bases:

>100_bases
ATTGGGTGCGACGCCCGCGGATCTGATGTCGATCCTGCAGGCGATGAAAGCGGCGGGCGCTCTGCGCGCCGACCTGGAAA
TCATCTAGGGAAGACACAGG

Downstream 100 bases:

>100_bases
GAGATGGGCTGAGCGGCCGTCGCGCCGTTCGAACCATCCGCTCATTCAATCGAATAAAACCATGCACGCGACGGGGCCAG
GTGCATAAAAAGACGGACGA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNSDTTNSAASANDLTQRFALDVQGFAKLSAQAKASPQAGMKMAAQQFDAVFTQMMLKSMRDATPQDGPFDSHDSATFTS
MMDQQLSQQLSQKGIGVADAMLKQLMRNQGMQVGGGAGAAGGLAGMANALGGGSGGDEGQTAALNALARAYGNAQANGQL
AIGRGYSANSALTPPLKGDGSSPKVDAFVDKLAGPAQAASAATGIPARFIIGQAALESGWGKSEIRKTDGSTSHNVFGIK
ATKDWTGKTVSTVTTEYVNGKPQRTVEKFRAYDSYQEAMTDYASLLKGNPRYAQVINSAHDVNGFANGMQRAGYATDPHY
AKKLMSIMQKMG

Sequences:

>Translated_332_residues
MNSDTTNSAASANDLTQRFALDVQGFAKLSAQAKASPQAGMKMAAQQFDAVFTQMMLKSMRDATPQDGPFDSHDSATFTS
MMDQQLSQQLSQKGIGVADAMLKQLMRNQGMQVGGGAGAAGGLAGMANALGGGSGGDEGQTAALNALARAYGNAQANGQL
AIGRGYSANSALTPPLKGDGSSPKVDAFVDKLAGPAQAASAATGIPARFIIGQAALESGWGKSEIRKTDGSTSHNVFGIK
ATKDWTGKTVSTVTTEYVNGKPQRTVEKFRAYDSYQEAMTDYASLLKGNPRYAQVINSAHDVNGFANGMQRAGYATDPHY
AKKLMSIMQKMG
>Mature_332_residues
MNSDTTNSAASANDLTQRFALDVQGFAKLSAQAKASPQAGMKMAAQQFDAVFTQMMLKSMRDATPQDGPFDSHDSATFTS
MMDQQLSQQLSQKGIGVADAMLKQLMRNQGMQVGGGAGAAGGLAGMANALGGGSGGDEGQTAALNALARAYGNAQANGQL
AIGRGYSANSALTPPLKGDGSSPKVDAFVDKLAGPAQAASAATGIPARFIIGQAALESGWGKSEIRKTDGSTSHNVFGIK
ATKDWTGKTVSTVTTEYVNGKPQRTVEKFRAYDSYQEAMTDYASLLKGNPRYAQVINSAHDVNGFANGMQRAGYATDPHY
AKKLMSIMQKMG

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=316, Percent_Identity=41.1392405063291, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34605; Mature: 34605

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDTTNSAASANDLTQRFALDVQGFAKLSAQAKASPQAGMKMAAQQFDAVFTQMMLKSM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDATPQDGPFDSHDSATFTSMMDQQLSQQLSQKGIGVADAMLKQLMRNQGMQVGGGAGAA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCCH
GGLAGMANALGGGSGGDEGQTAALNALARAYGNAQANGQLAIGRGYSANSALTPPLKGDG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCC
SSPKVDAFVDKLAGPAQAASAATGIPARFIIGQAALESGWGKSEIRKTDGSTSHNVFGIK
CCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEE
ATKDWTGKTVSTVTTEYVNGKPQRTVEKFRAYDSYQEAMTDYASLLKGNPRYAQVINSAH
ECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DVNGFANGMQRAGYATDPHYAKKLMSIMQKMG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSDTTNSAASANDLTQRFALDVQGFAKLSAQAKASPQAGMKMAAQQFDAVFTQMMLKSM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDATPQDGPFDSHDSATFTSMMDQQLSQQLSQKGIGVADAMLKQLMRNQGMQVGGGAGAA
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEECCCCCCH
GGLAGMANALGGGSGGDEGQTAALNALARAYGNAQANGQLAIGRGYSANSALTPPLKGDG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCC
SSPKVDAFVDKLAGPAQAASAATGIPARFIIGQAALESGWGKSEIRKTDGSTSHNVFGIK
CCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEE
ATKDWTGKTVSTVTTEYVNGKPQRTVEKFRAYDSYQEAMTDYASLLKGNPRYAQVINSAH
ECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DVNGFANGMQRAGYATDPHYAKKLMSIMQKMG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]