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The map label for this gene is pilQ [H]

Identifier: 91785421

GI number: 91785421

Start: 4479902

End: 4481605

Strand: Reverse

Name: pilQ [H]

Synonym: Bxe_A0358

Alternate gene names: 91785421

Gene position: 4481605-4479902 (Counterclockwise)

Preceding gene: 91785422

Following gene: 91785420

Centisome position: 91.54

GC content: 67.55

Gene sequence:

>1704_bases
ATGAGACTCGCGAAGCCATTTCCGGTTGGATTGTTGGGCGTCGGCGCAGGTGTATCGCCCGGACTGAAAATATGTCTCGC
TCTGTGTGTCAGCGCGGCGCTGGCTGTCGGGGGTGCGAGCGCGTCGACTCCACCGTTGCCGCCGTTGCCTGCCGGGATGC
CGCTCGACGGTGCGGTTATGCCAGCCGATCCTCCGTACAGCGCGCCATCGCTGCCACGCGAAGTTACCGCCGACGTGCCC
AATCCGTTTCGTCAGGATTCCGCAGACGATCCAGACGCATCGCCTGCCAGTGCGACGGCCGCCGATGCCGCCGCGGACGG
GCCTGCCACCGACGTCAACGCCGCCACCGCCGCTGACGGCTCCGCGCCGCTACGCAGGCAATCTGCCGCAGCGGCGCGCG
ACAATGCCGCCGGCAGCAGCGCGCTCGAAGGTCCGCCGGTGCCGCTGCCGCCGCTCGCGCGTCTGAGCGGCGCGCCGCTC
AGTGCCGCCGATGCCGGCCTCGCCCCCGACGACAAACCGCTCTCGCTGAATTTTCAGCGTGCCGAACTCGGCGCCGTGCT
CAATGCGTTTGCCAGGTTCACGGGGCTGAACATCGTTGCGAGCGAGAGAGTGCGCGGTGCAGTCTCGCTACGGCTCGACA
AGGTGCCGTGGCGCACCGCCTTCGACACCCTGCTCGACGTCAACGGCCTCGCGATGGAGCGGCACGGCAACGTGATCTGG
GTCGCGCCGATTGCGGATCTGGCTGCGCGCGAGCGGCAGCGCTTCGAAGCGCACGCCCGGGCCGCCGATCTCGAGCCGCT
CGCCAGCCGCACGTTCGAACTGCACTACGCGCATGCGGAGGACGTCAGGCGCCTGCTGACCGGTTCGGGCGCCCAGCGCG
TCCTGTCCAAACGTGGTGCCGCGACCGCCGATCCGCGCACCAATCTGCTGTTCGTGACCGATCTGGAAGCGCGCCTCGCG
CAGATTGCGGCGCTGCTCACCTCGGTTGACCGGCCGACGCGCCAGGTATTGATCGAAGCGCGTATCGTCGAGGGCGAACA
CGGTTTTTCGCGCAATCTCGGCGTGAAGCTCTCGATGGCGACCAGCAACGCCGACGGCAGCGCGAAAGGTTTGACCGACG
CCGCCTACGATCTGTCGGCCCGCCCGATCGCCGGTTTCGATGCGGCGACCGCCGGTCTGACGTTATTCGCCGCCGGCGCG
ACGCGCTTGCTGAACATCGAGCTGAGCGCGCTCGAAGCACAGGGGCGCGGCGAAATCGTTTCGAGCCCGCGCGTGGTCAC
GGCGGACCGGATGAAGGCGGTGGTCGAGCAGGGCACCGAACTGCCGTATCAGGCGAAAGTGGGGCAGGGCGTATCGGGCG
TGCAGTTTCGGCGCGCCACGCTCAAACTGGAGGTCGAGCCGCAAATCATGCCGGACGGCCGTGTGGTGCTGGACCTCGAC
GTCGCCAAAGACAGCGTCGGCGAACAGACCGACGCTGGGCCTGCGATCAACACCAAACACGTGCAAACGCGCGTCGAAGT
CGAGGATGGTGGTACGGTGTCGATCGGCGGCATTTACGCGACCGACGACCGGGACGATGTGACGCAGGTGCCGCTCCTGG
GCAAAATACCGGTTTTGGGCGCGCTTTTCCGCCATCGGGCTCATCGCGACCGGCGCAGCGAGCTGGCCGTGTTCATCACG
CCGCGCGTCGTTCAGACAAATTAG

Upstream 100 bases:

>100_bases
AGCAGATCGGCGTGGAACGTGTCACGAGGCTGGACGCGTTCGGCATTACGCTCGCGAAGGGCAGCGCGACGCGCATGCTG
GCGCTGACGGAGACTTCGTG

Downstream 100 bases:

>100_bases
GGCACGCTCCTGCCTCTGCCGGGAAAAACGCAGGGCGGCCCTAAGTTTTCCGCTTTACAGCGCGCCCACCCGGCGATTCC
CATTGGGGCGCCGGGCGCGA

Product: putative fimbrial type-4 assembly signal peptide, PilQ-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MRLAKPFPVGLLGVGAGVSPGLKICLALCVSAALAVGGASASTPPLPPLPAGMPLDGAVMPADPPYSAPSLPREVTADVP
NPFRQDSADDPDASPASATAADAAADGPATDVNAATAADGSAPLRRQSAAAARDNAAGSSALEGPPVPLPPLARLSGAPL
SAADAGLAPDDKPLSLNFQRAELGAVLNAFARFTGLNIVASERVRGAVSLRLDKVPWRTAFDTLLDVNGLAMERHGNVIW
VAPIADLAARERQRFEAHARAADLEPLASRTFELHYAHAEDVRRLLTGSGAQRVLSKRGAATADPRTNLLFVTDLEARLA
QIAALLTSVDRPTRQVLIEARIVEGEHGFSRNLGVKLSMATSNADGSAKGLTDAAYDLSARPIAGFDAATAGLTLFAAGA
TRLLNIELSALEAQGRGEIVSSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLEVEPQIMPDGRVVLDLD
VAKDSVGEQTDAGPAINTKHVQTRVEVEDGGTVSIGGIYATDDRDDVTQVPLLGKIPVLGALFRHRAHRDRRSELAVFIT
PRVVQTN

Sequences:

>Translated_567_residues
MRLAKPFPVGLLGVGAGVSPGLKICLALCVSAALAVGGASASTPPLPPLPAGMPLDGAVMPADPPYSAPSLPREVTADVP
NPFRQDSADDPDASPASATAADAAADGPATDVNAATAADGSAPLRRQSAAAARDNAAGSSALEGPPVPLPPLARLSGAPL
SAADAGLAPDDKPLSLNFQRAELGAVLNAFARFTGLNIVASERVRGAVSLRLDKVPWRTAFDTLLDVNGLAMERHGNVIW
VAPIADLAARERQRFEAHARAADLEPLASRTFELHYAHAEDVRRLLTGSGAQRVLSKRGAATADPRTNLLFVTDLEARLA
QIAALLTSVDRPTRQVLIEARIVEGEHGFSRNLGVKLSMATSNADGSAKGLTDAAYDLSARPIAGFDAATAGLTLFAAGA
TRLLNIELSALEAQGRGEIVSSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLEVEPQIMPDGRVVLDLD
VAKDSVGEQTDAGPAINTKHVQTRVEVEDGGTVSIGGIYATDDRDDVTQVPLLGKIPVLGALFRHRAHRDRRSELAVFIT
PRVVQTN
>Mature_567_residues
MRLAKPFPVGLLGVGAGVSPGLKICLALCVSAALAVGGASASTPPLPPLPAGMPLDGAVMPADPPYSAPSLPREVTADVP
NPFRQDSADDPDASPASATAADAAADGPATDVNAATAADGSAPLRRQSAAAARDNAAGSSALEGPPVPLPPLARLSGAPL
SAADAGLAPDDKPLSLNFQRAELGAVLNAFARFTGLNIVASERVRGAVSLRLDKVPWRTAFDTLLDVNGLAMERHGNVIW
VAPIADLAARERQRFEAHARAADLEPLASRTFELHYAHAEDVRRLLTGSGAQRVLSKRGAATADPRTNLLFVTDLEARLA
QIAALLTSVDRPTRQVLIEARIVEGEHGFSRNLGVKLSMATSNADGSAKGLTDAAYDLSARPIAGFDAATAGLTLFAAGA
TRLLNIELSALEAQGRGEIVSSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLEVEPQIMPDGRVVLDLD
VAKDSVGEQTDAGPAINTKHVQTRVEVEDGGTVSIGGIYATDDRDDVTQVPLLGKIPVLGALFRHRAHRDRRSELAVFIT
PRVVQTN

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=391, Percent_Identity=35.5498721227622, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI87082242, Length=353, Percent_Identity=29.4617563739377, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 59074; Mature: 59074

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAKPFPVGLLGVGAGVSPGLKICLALCVSAALAVGGASASTPPLPPLPAGMPLDGAVM
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PADPPYSAPSLPREVTADVPNPFRQDSADDPDASPASATAADAAADGPATDVNAATAADG
CCCCCCCCCCCCHHHCCCCCCCHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
SAPLRRQSAAAARDNAAGSSALEGPPVPLPPLARLSGAPLSAADAGLAPDDKPLSLNFQR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEHH
AELGAVLNAFARFTGLNIVASERVRGAVSLRLDKVPWRTAFDTLLDVNGLAMERHGNVIW
HHHHHHHHHHHHHHCCEEEEHHHHCCEEEEEEECCCCHHHHHHHHCCCCEEEECCCCEEE
VAPIADLAARERQRFEAHARAADLEPLASRTFELHYAHAEDVRRLLTGSGAQRVLSKRGA
EECHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEEECCHHHHHHHHCCCCHHHHHHHCCC
ATADPRTNLLFVTDLEARLAQIAALLTSVDRPTRQVLIEARIVEGEHGFSRNLGVKLSMA
CCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCCEEEEEE
TSNADGSAKGLTDAAYDLSARPIAGFDAATAGLTLFAAGATRLLNIELSALEAQGRGEIV
ECCCCCCCCCCCHHHHCCCCCCCCCCCHHHHCEEEEECCCEEEEEEEEEEEECCCCCCEE
SSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLEVEPQIMPDGRVVLDLD
CCCEEEEHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEEEEEEECCEECCCCCEEEEEE
VAKDSVGEQTDAGPAINTKHVQTRVEVEDGGTVSIGGIYATDDRDDVTQVPLLGKIPVLG
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCEEECCCCCCHHCCCCCCCCHHHH
ALFRHRAHRDRRSELAVFITPRVVQTN
HHHHHHHHHHHHCCEEEEEECEEEECC
>Mature Secondary Structure
MRLAKPFPVGLLGVGAGVSPGLKICLALCVSAALAVGGASASTPPLPPLPAGMPLDGAVM
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
PADPPYSAPSLPREVTADVPNPFRQDSADDPDASPASATAADAAADGPATDVNAATAADG
CCCCCCCCCCCCHHHCCCCCCCHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCC
SAPLRRQSAAAARDNAAGSSALEGPPVPLPPLARLSGAPLSAADAGLAPDDKPLSLNFQR
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEHH
AELGAVLNAFARFTGLNIVASERVRGAVSLRLDKVPWRTAFDTLLDVNGLAMERHGNVIW
HHHHHHHHHHHHHHCCEEEEHHHHCCEEEEEEECCCCHHHHHHHHCCCCEEEECCCCEEE
VAPIADLAARERQRFEAHARAADLEPLASRTFELHYAHAEDVRRLLTGSGAQRVLSKRGA
EECHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEEECCHHHHHHHHCCCCHHHHHHHCCC
ATADPRTNLLFVTDLEARLAQIAALLTSVDRPTRQVLIEARIVEGEHGFSRNLGVKLSMA
CCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCCCCCCCCEEEEEE
TSNADGSAKGLTDAAYDLSARPIAGFDAATAGLTLFAAGATRLLNIELSALEAQGRGEIV
ECCCCCCCCCCCHHHHCCCCCCCCCCCHHHHCEEEEECCCEEEEEEEEEEEECCCCCCEE
SSPRVVTADRMKAVVEQGTELPYQAKVGQGVSGVQFRRATLKLEVEPQIMPDGRVVLDLD
CCCEEEEHHHHHHHHHCCCCCCCHHHCCCCCCCCEEEEEEEEEEECCEECCCCCEEEEEE
VAKDSVGEQTDAGPAINTKHVQTRVEVEDGGTVSIGGIYATDDRDDVTQVPLLGKIPVLG
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCEEECCCCCCHHCCCCCCCCHHHH
ALFRHRAHRDRRSELAVFITPRVVQTN
HHHHHHHHHHHHCCEEEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]