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The map label for this gene is gltD [H]

Identifier: 91785413

GI number: 91785413

Start: 4467675

End: 4469144

Strand: Reverse

Name: gltD [H]

Synonym: Bxe_A0366

Alternate gene names: 91785413

Gene position: 4469144-4467675 (Counterclockwise)

Preceding gene: 91785414

Following gene: 91785412

Centisome position: 91.28

GC content: 62.93

Gene sequence:

>1470_bases
ATGGGCAAGGCAACCGGTTTTCTCGAGTTCGAACGCCGCCACGAGGCGTACGAAGCTCCGCTCACGCGTGTGAAGCACTA
CAAGGAATTCGTCGCCGCACTGAGCGACGAGGAAGCGAAGATTCAAGGCGCACGTTGCATGGACTGCGGCATTCCGTTCT
GCAACAACGGCTGCCCGGTGAACAACATCATCCCGGACTTCAACGACCTGGTGTTTCATCAGGACTGGAAGAACGCGATC
GAAGTGCTGCACTCCACCAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAAGCGGCGTGCACGCT
CGGCATCAACAATGACCCGGTCGGCATCAAGTCGATCGAACACGCGATCATCGACAAGGCATGGACCGAAGGCTGGGTCG
CGCCGCTGCCGCCGAAGCACAAGACCGGCAAGAAGGTCGCCGTGGTCGGATCCGGCCCCGCTGGTTTGGCTGTCGCGCAG
CAACTCGCGCGCGCGGGGCACGATGTCACCGTGTTCGAGAAGAACGACCGCATCGGCGGCTTGCTGCGTTACGGCATTCC
CGACTTCAAGCTCGAGAAGTGGCTGATCGATCGCCGCATGCGCCAGATGGAAGCCGAAGGCGTGACGTTCCGCGCCAACG
TGTTCATCGGCAAGGCGGATTCGCTGCCGGCGCATATCGGCAACACGGCGAAGGAAACCATCACGCCGGATCAGTTGAAG
GAAGAGTTCGACGCAGTGGTGATCGCGGGCGGTTCGGAAACGCCGCGCGATCTGCCGGTGCCGGGCCGCGAGCTGGAAGG
CATCCACTACGCGATGGAATTCCTGCCGCAACAGAACAAGGTCAATGCCGGCGACAAGGTCGCGAACCAGCTGCTCGCGA
AGGGCAAGCATGTGGTCGTGATCGGCGGCGGCGATACGGGTTCGGACTGCGTGGGCACCTCGAATCGTCATGGCGCGAAG
AGCGTCACGCAATTCGAACTGCTGCCGCAACCGCCGGAAGAAGAGAACAAGCCGCTTGTGTGGCCGTACTGGCCGATCAA
GCTGCGCACGTCGTCGTCGCATGACGAAGGCTGCTCGCGTGACTGGGCGGTCGCGACCAAGCGCCTCGAAGGCAAGAACG
GCAAGGTCGAGAAGCTGATCGCGGCGCGCGTCGAATGGAAGGACGGCAAGATGCAGGAAGTGCCGGGCTCCGAATTCGAA
ATGAAGGCCGATCTGGTGCTGCTCGCGATGGGTTTCACGCAGCCGGTCTCGCCGGTGCTCGAAGCGTTCGGCGTCGACAA
GGATGCGCGCGGCAACGTGCGTTCGTCGACCGAAGGCGACAAGGCGTATTACACGTCGGTGGACAAAGTGTTCACCGCGG
GCGACATGCGTCGCGGCCAGTCGCTGGTGGTGTGGGCGATTCGCGAAGGCCGCCAGTGCGCGCGTTCCGTCGACGCGTTC
CTGATGGGTCATTCGGAACTGCCGCGCTAA

Upstream 100 bases:

>100_bases
AGACGTCACAACCAAGCCGTACCCGCCGCGTGGCGCCGTTGATCGGTCGCACGCGGTGGGTGTAGATCACCCCACCTGAT
TAGATAGAGAAGAGAACCAC

Downstream 100 bases:

>100_bases
ACGTCTCCGGCCGGCTTCGCGCTTTGGCGCGAAAGCCGGCTACTGCTTGCAGGAAGAGGTGGAGACGACAGACGTGCTAC
GTGAGCGGCACATCGAGCGG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDEEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVFHQDWKNAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIEHAIIDKAWTEGWVAPLPPKHKTGKKVAVVGSGPAGLAVAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKADSLPAHIGNTAKETITPDQLK
EEFDAVVIAGGSETPRDLPVPGRELEGIHYAMEFLPQQNKVNAGDKVANQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAK
SVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDWAVATKRLEGKNGKVEKLIAARVEWKDGKMQEVPGSEFE
MKADLVLLAMGFTQPVSPVLEAFGVDKDARGNVRSSTEGDKAYYTSVDKVFTAGDMRRGQSLVVWAIREGRQCARSVDAF
LMGHSELPR

Sequences:

>Translated_489_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDEEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVFHQDWKNAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIEHAIIDKAWTEGWVAPLPPKHKTGKKVAVVGSGPAGLAVAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKADSLPAHIGNTAKETITPDQLK
EEFDAVVIAGGSETPRDLPVPGRELEGIHYAMEFLPQQNKVNAGDKVANQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAK
SVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDWAVATKRLEGKNGKVEKLIAARVEWKDGKMQEVPGSEFE
MKADLVLLAMGFTQPVSPVLEAFGVDKDARGNVRSSTEGDKAYYTSVDKVFTAGDMRRGQSLVVWAIREGRQCARSVDAF
LMGHSELPR
>Mature_488_residues
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDEEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVFHQDWKNAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIEHAIIDKAWTEGWVAPLPPKHKTGKKVAVVGSGPAGLAVAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKADSLPAHIGNTAKETITPDQLKE
EFDAVVIAGGSETPRDLPVPGRELEGIHYAMEFLPQQNKVNAGDKVANQLLAKGKHVVVIGGGDTGSDCVGTSNRHGAKS
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSRDWAVATKRLEGKNGKVEKLIAARVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPVSPVLEAFGVDKDARGNVRSSTEGDKAYYTSVDKVFTAGDMRRGQSLVVWAIREGRQCARSVDAFL
MGHSELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=493, Percent_Identity=25.1521298174442, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1789606, Length=487, Percent_Identity=34.2915811088296, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1788811, Length=468, Percent_Identity=35.6837606837607, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI87082180, Length=468, Percent_Identity=33.1196581196581, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788468, Length=462, Percent_Identity=26.8398268398268, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789243, Length=439, Percent_Identity=26.879271070615, Blast_Score=116, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17570289, Length=509, Percent_Identity=45.5795677799607, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71984108, Length=480, Percent_Identity=25.4166666666667, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17543860, Length=180, Percent_Identity=31.6666666666667, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320030, Length=503, Percent_Identity=49.3041749502982, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574881, Length=504, Percent_Identity=48.8095238095238, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665539, Length=504, Percent_Identity=48.8095238095238, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24665547, Length=504, Percent_Identity=48.8095238095238, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24665543, Length=504, Percent_Identity=48.8095238095238, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI24640763, Length=487, Percent_Identity=23.2032854209446, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18858217, Length=487, Percent_Identity=23.2032854209446, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53752; Mature: 53620

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDEEAKIQGARCMDCGIPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVFHQDWKNAIEVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWTEGWVAPLPPKHKTGKKVAVVGSGPAGLAVAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKADSLPAHIGNTAKETITPDQLK
EEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHCCCCHHHCCCHHHHH
EEFDAVVIAGGSETPRDLPVPGRELEGIHYAMEFLPQQNKVNAGDKVANQLLAKGKHVVV
HHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEE
IGGGDTGSDCVGTSNRHGAKSVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSR
EECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCC
DWAVATKRLEGKNGKVEKLIAARVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPVSPVL
CHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEECCEEEEEECCCCCHHHHH
EAFGVDKDARGNVRSSTEGDKAYYTSVDKVFTAGDMRRGQSLVVWAIREGRQCARSVDAF
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCEEEEEEEHHHHHHHHHHHHH
LMGHSELPR
HHCCCCCCC
>Mature Secondary Structure 
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALSDEEAKIQGARCMDCGIPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVFHQDWKNAIEVLHSTNNFPEFTGRICPAPCEAACTLGINNDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWTEGWVAPLPPKHKTGKKVAVVGSGPAGLAVAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRANVFIGKADSLPAHIGNTAKETITPDQLK
EEECCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHCCCCHHHCCCHHHHH
EEFDAVVIAGGSETPRDLPVPGRELEGIHYAMEFLPQQNKVNAGDKVANQLLAKGKHVVV
HHHCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEE
IGGGDTGSDCVGTSNRHGAKSVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHDEGCSR
EECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCCCCCCCCC
DWAVATKRLEGKNGKVEKLIAARVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPVSPVL
CHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEECCEEEEEECCCCCHHHHH
EAFGVDKDARGNVRSSTEGDKAYYTSVDKVFTAGDMRRGQSLVVWAIREGRQCARSVDAF
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCEEEEEEEHHHHHHHHHHHHH
LMGHSELPR
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]