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The map label for this gene is ddl [H]

Identifier: 91785291

GI number: 91785291

Start: 4339492

End: 4340433

Strand: Reverse

Name: ddl [H]

Synonym: Bxe_A0488

Alternate gene names: 91785291

Gene position: 4340433-4339492 (Counterclockwise)

Preceding gene: 91785292

Following gene: 91785290

Centisome position: 88.66

GC content: 63.59

Gene sequence:

>942_bases
ATGAGCAGTATCGACCCCAAACAGTTCGGCAAGGTGGCAGTGCTGCTCGGCGGTAATTCCGCCGAGCGCGAGGTGTCGCT
CAATTCCGGGCGTCTCGTGCTTCAAGGTCTGCGCGACGCCGGCATCGACGCGCATCCGTTCGATCCGGCCGAGCGTCCGC
TCGCCGCATTGAAGGAAGAGGGCTTCGTGCGCGCGTTCAACGCGCTGCACGGCGGCTACGGCGAGAACGGCCAGATCCAG
GGCGCGCTCGATTTTTACGGCATCCGCTACACGGGCAGCGGCGTGCTCGGCTCGGCGCTCGGTCTGGACAAGTTCCGCAC
CAAGCTCGTGTGGCAGCAACTCGGTATTCCGACGCCGCCGTTCGAAGCCGTGCTGCGCGGCGACGACTACGAAGCGCGCG
CCAAGGAGATCGTCGCGAAGCTCGGTTTGCCGCTTTTCGTGAAGCCGGCGAGCGAAGGTTCGAGCGTCGCGGTAATCAAG
GTGAAAAGCGCAGACGCGCTGCCCGCCGCGTTGATCGAAGCAGTGAAGTTCGACAGGATCGTGGTGGTCGAAAAGAGTAT
TGAAGGCGGCGGCGAATACACCGCGTGCATCGCCGGCAATCTGGATTTGCCGGTGATCCGCATCGTGCCGGCCGGCGAGT
TTTACGATTACCACGCGAAGTACATCGCGAACGACACGCAGTATCTGATTCCGTGCGGTCTGACGGCGGACGAGGAGGCG
CGTCTGAAAGTGCTGGCGCGCCGTGCCTTCGACGTGCTCGGCTGCACCGACTGGGGCCGCGCGGATTTCATGCTCGACGC
CGACGGCAACCCGTATTTCCTCGAAGTGAACACGGCGCCCGGCATGACCGATCACTCGCTGCCGCCGAAAGCGGCGAGAG
CGGTGGGCATCAGTTATCAGGAACTCGTTGTCGCCGTGTTGGCGCTGACGCTCAAGGACTAA

Upstream 100 bases:

>100_bases
CGGCGATAGTGCGCGACGGCGATGTGGTGATCACGATGGGCGCAGGTTCGATCGGCGGTGTGCCGGCTCGCCTCGCGCAG
GAAACAAAGGTGTGAGTGAG

Downstream 100 bases:

>100_bases
CCGGGGCGACCCAAAAGCAACACCATGTGGAACAACGTTCGCCAGCTCAATTTCGCCTCCAACGCATTGCATGTGTTGCT
GGTGCTTGTGCTGCTGGCGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSSIDPKQFGKVAVLLGGNSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAERPLAALKEEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPPFEAVLRGDDYEARAKEIVAKLGLPLFVKPASEGSSVAVIK
VKSADALPAALIEAVKFDRIVVVEKSIEGGGEYTACIAGNLDLPVIRIVPAGEFYDYHAKYIANDTQYLIPCGLTADEEA
RLKVLARRAFDVLGCTDWGRADFMLDADGNPYFLEVNTAPGMTDHSLPPKAARAVGISYQELVVAVLALTLKD

Sequences:

>Translated_313_residues
MSSIDPKQFGKVAVLLGGNSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAERPLAALKEEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPPFEAVLRGDDYEARAKEIVAKLGLPLFVKPASEGSSVAVIK
VKSADALPAALIEAVKFDRIVVVEKSIEGGGEYTACIAGNLDLPVIRIVPAGEFYDYHAKYIANDTQYLIPCGLTADEEA
RLKVLARRAFDVLGCTDWGRADFMLDADGNPYFLEVNTAPGMTDHSLPPKAARAVGISYQELVVAVLALTLKD
>Mature_312_residues
SSIDPKQFGKVAVLLGGNSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAERPLAALKEEGFVRAFNALHGGYGENGQIQG
ALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPPFEAVLRGDDYEARAKEIVAKLGLPLFVKPASEGSSVAVIKV
KSADALPAALIEAVKFDRIVVVEKSIEGGGEYTACIAGNLDLPVIRIVPAGEFYDYHAKYIANDTQYLIPCGLTADEEAR
LKVLARRAFDVLGCTDWGRADFMLDADGNPYFLEVNTAPGMTDHSLPPKAARAVGISYQELVVAVLALTLKD

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=52.317880794702, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=32.8571428571429, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33536; Mature: 33404

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIDPKQFGKVAVLLGGNSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAERPLAALKEE
CCCCCCHHCCCEEEEECCCCCCCEEECCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHC
GFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHHHCCCCCC
FEAVLRGDDYEARAKEIVAKLGLPLFVKPASEGSSVAVIKVKSADALPAALIEAVKFDRI
HHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCEE
VVVEKSIEGGGEYTACIAGNLDLPVIRIVPAGEFYDYHAKYIANDTQYLIPCGLTADEEA
EEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCHHCCHHEEECCCCEEEEECCCCCCHH
RLKVLARRAFDVLGCTDWGRADFMLDADGNPYFLEVNTAPGMTDHSLPPKAARAVGISYQ
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVAVLALTLKD
HHHHHHHHHHCCC
>Mature Secondary Structure 
SSIDPKQFGKVAVLLGGNSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAERPLAALKEE
CCCCCHHCCCEEEEECCCCCCCEEECCCCEEEEEEHHHCCCCCCCCCCHHHHHHHHHHC
GFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQLGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHHHCCCCCC
FEAVLRGDDYEARAKEIVAKLGLPLFVKPASEGSSVAVIKVKSADALPAALIEAVKFDRI
HHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCEE
VVVEKSIEGGGEYTACIAGNLDLPVIRIVPAGEFYDYHAKYIANDTQYLIPCGLTADEEA
EEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCHHCCHHEEECCCCEEEEECCCCCCHH
RLKVLARRAFDVLGCTDWGRADFMLDADGNPYFLEVNTAPGMTDHSLPPKAARAVGISYQ
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVAVLALTLKD
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA