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The map label for this gene is ampD [H]

Identifier: 91785260

GI number: 91785260

Start: 4307813

End: 4308409

Strand: Reverse

Name: ampD [H]

Synonym: Bxe_A0519

Alternate gene names: 91785260

Gene position: 4308409-4307813 (Counterclockwise)

Preceding gene: 91785261

Following gene: 91785259

Centisome position: 88.0

GC content: 63.99

Gene sequence:

>597_bases
ATGAGCGTCGAGTTCACCGTCGACGCCGACGGTTGGGTCGCCGCCGCACGCAAACTGCCATCGCCGAATTTCGAGGCGCG
GCCTGAAGGAGCCGAGCCGACGCTGATCGTCGTCCACAACATCAGCCTGCCGCCCAACGAATTCGGCGGCACCGCAATCG
CCGAACTGTTCCTCAATACGCTCGACTGCAACGCTCACCCGTATTACGACTCGCATCTGCGCGGCGTGCGGGTGTCGGCG
CATTTCGTGATTCATCGCGACGGCGCGCTCGAGCAGTTCGTGTCGTGCAACGAGCGTGCGTGGCACGCCGGGCCGTCGAA
TTTTTTCGGCCGCGAGCGTTGCAATGATTTCTCGATCGGCATCGAGCTGGAGGGCAGCGACACCACGCCTTTCGAAGCGG
CGCAATACCGCACGCTCGGCGCCCTCGTGAGCGCGCTCAAGGCGCGCTATCCGGTCACGGCGCTTGCCGGTCACTCGGAC
ATCGCCCCCGGTCGCAAGACCGATCCAGGGCCGCATTTCGAGTGGTCGCGCCTGCAGCGCGACGCGTCGCTCGACGCTCA
GTATTTCCCCTATCTCAAGTTTTCCAAAACGCCGTAA

Upstream 100 bases:

>100_bases
ACGCGCCGAAGAGCGATTCCGTGGTGGTGGCCGGGCAGCCGTTCTGCTGCGCCGCGCACGCGCAGCGTCACGGCGCGCGA
CCCACCGGCCGCGACGCTCG

Downstream 100 bases:

>100_bases
CGGCCCTTCGTGCGACTTCGCGTGATGCGAGGAGAACAAGGCGCTGCCTTGTTCTCCGGGGAGTCGGCTTCGCGCCGTGT
TTGCGCCGCACCAGGCAAAA

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSVEFTVDADGWVAAARKLPSPNFEARPEGAEPTLIVVHNISLPPNEFGGTAIAELFLNTLDCNAHPYYDSHLRGVRVSA
HFVIHRDGALEQFVSCNERAWHAGPSNFFGRERCNDFSIGIELEGSDTTPFEAAQYRTLGALVSALKARYPVTALAGHSD
IAPGRKTDPGPHFEWSRLQRDASLDAQYFPYLKFSKTP

Sequences:

>Translated_198_residues
MSVEFTVDADGWVAAARKLPSPNFEARPEGAEPTLIVVHNISLPPNEFGGTAIAELFLNTLDCNAHPYYDSHLRGVRVSA
HFVIHRDGALEQFVSCNERAWHAGPSNFFGRERCNDFSIGIELEGSDTTPFEAAQYRTLGALVSALKARYPVTALAGHSD
IAPGRKTDPGPHFEWSRLQRDASLDAQYFPYLKFSKTP
>Mature_197_residues
SVEFTVDADGWVAAARKLPSPNFEARPEGAEPTLIVVHNISLPPNEFGGTAIAELFLNTLDCNAHPYYDSHLRGVRVSAH
FVIHRDGALEQFVSCNERAWHAGPSNFFGRERCNDFSIGIELEGSDTTPFEAAQYRTLGALVSALKARYPVTALAGHSDI
APGRKTDPGPHFEWSRLQRDASLDAQYFPYLKFSKTP

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=171, Percent_Identity=55.5555555555556, Blast_Score=197, Evalue=3e-52,
Organism=Escherichia coli, GI1787092, Length=124, Percent_Identity=39.5161290322581, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21862; Mature: 21730

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEFTVDADGWVAAARKLPSPNFEARPEGAEPTLIVVHNISLPPNEFGGTAIAELFLNT
CCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
LDCNAHPYYDSHLRGVRVSAHFVIHRDGALEQFVSCNERAWHAGPSNFFGRERCNDFSIG
HCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
IELEGSDTTPFEAAQYRTLGALVSALKARYPVTALAGHSDIAPGRKTDPGPHFEWSRLQR
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHH
DASLDAQYFPYLKFSKTP
CCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
SVEFTVDADGWVAAARKLPSPNFEARPEGAEPTLIVVHNISLPPNEFGGTAIAELFLNT
CEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
LDCNAHPYYDSHLRGVRVSAHFVIHRDGALEQFVSCNERAWHAGPSNFFGRERCNDFSIG
HCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEE
IELEGSDTTPFEAAQYRTLGALVSALKARYPVTALAGHSDIAPGRKTDPGPHFEWSRLQR
EEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHH
DASLDAQYFPYLKFSKTP
CCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]