Click here to switch to the map view.

The map label for this gene is mpl [H]

Identifier: 91785243

GI number: 91785243

Start: 4291141

End: 4292535

Strand: Reverse

Name: mpl [H]

Synonym: Bxe_A0536

Alternate gene names: 91785243

Gene position: 4292535-4291141 (Counterclockwise)

Preceding gene: 91785254

Following gene: 91785242

Centisome position: 87.68

GC content: 65.73

Gene sequence:

>1395_bases
ATGCACATCCACATCCTCGGTATTTGCGGCACGTTCATGGGCGGTCTCGCGGTCCTCGCGCGCGGCGCGGGCCACACCGT
GACGGGTTGCGACGCCGGCGTCTATCCGCCCATGAGCACGCAGCTGGAGGCGCAAGGCATTGGTCTGATCGAAGGTTACG
ACGCGGACCAGCTGAACGGTCTGAACGCGGATCTGTTCGTGATCGGCAACGTGGTCTCACGCGGCAACCCGCTGATGGAG
GCCATTCTCGACCGCGGCCTGCCGTATACCTCCGGTCCGCAATGGCTCGGCGAGCATGTGCTGAACGGCAAATGGGTGCT
GGCGGTGGCCGGCACGCACGGCAAGACGACCACCAGCTCCATGCTGACATGGCTGCTCGAAGACGCCGGCCTCAATCCGG
GCTTCCTGATCGGCGGTGTGCCGCTGAATTTCGGCGTGTCGGCCCGGCTGACCGATTCCAGCTTCTTCGTGATCGAAGCC
GACGAATACGACACGGCCTTTTTCGATAAGCGCTCGAAGTTCGTCCACTACCGGCCCAAAACCGCCGTGCTGAACAACCT
CGAATTCGATCACGCGGATATTTTCCCCGATCTGGCCGCGATCGAAACTCAGTTCCACCACCTGATCCGCACCGTGCCGG
GCATTGGCCGGATCGTGACGAACGGCCGTGAAGACGCGCTCGAGCGCGTGCTGACGCGCGGTTGCTGGAGCGAAGTGGAG
CGCTTCGGCGTGCAGGGCGGCTGGGAAACACTGCCGGCCGAAGACGGCGTGCCGGTCGACGAGCGTTTCGCCGTCTATCA
CAACAGTGAGCGCGTCGGCGCGGTCGAGTGGCAAGTGCAAGGCGAGCACAACCGCATGAACGCGCTGGCCGCGATCGCCG
CCGCGCGCCATGTCGGCGTGCCGCCGGCGCAAGCCGCCCGGTCGCTGGCGAGCTTTCGCAACGTCAAGCGCCGCATGGAG
GTGCGCGGCAGCGTCGACGGCGTCACCGTCTATGACGACTTCGCCCATCATCCCACCGCCATCGAGACCACCCTGGCCGG
GCTGCGCGCCCGCGTGGGCCGCGACAACACGCGCATTCTCGCCGTACTGGAACCGCGCTCCAATACCATGAAGCTTGGTG
TGATGAAAGCGCAGTTGCCGGCCAGCCTTGCCGACGCCGATCTCGTCTTCGGCTACGGCGCGCCGACCGGCCGCGACGCG
CTCGGCTGGAATCTCGGCGAAGCGCTCGCGCCGCTCGGCGGCAAGGCGCAGGCTTTCGACAATCTCGACGCGCTGGTCAA
AGCGGTAGTTCACACAGCGCGCCCCGGCGACCAGATTCTGGTGATGAGCAACGGCGGCTTCGGCGGCGTGCATCAGAAAC
TGCTCGATGCTCTTTCCGCGCGAGGCACTTCGTGA

Upstream 100 bases:

>100_bases
CCGGGCCGCCCCAGGTTTTCTTGCCCCCTCGGGGGCCTGGCGCGAAGCGGCAGGTTAGGGGGCCCTTCCAGGAAAAACGC
CCGCGCGGCACGACAGGCTT

Downstream 100 bases:

>100_bases
TCCTTTATCTGCACGGTTTCCGCTCGTCACCCAATTCGTTCAAGGCGCGCGTGCTGGCGGCACGGCTTGTCGAACTCGGC
CGCAGCGACGAATGGTGCTG

Product: UDP-N-acetylmuramate/L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, MurC

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MHIHILGICGTFMGGLAVLARGAGHTVTGCDAGVYPPMSTQLEAQGIGLIEGYDADQLNGLNADLFVIGNVVSRGNPLME
AILDRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTSSMLTWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEA
DEYDTAFFDKRSKFVHYRPKTAVLNNLEFDHADIFPDLAAIETQFHHLIRTVPGIGRIVTNGREDALERVLTRGCWSEVE
RFGVQGGWETLPAEDGVPVDERFAVYHNSERVGAVEWQVQGEHNRMNALAAIAAARHVGVPPAQAARSLASFRNVKRRME
VRGSVDGVTVYDDFAHHPTAIETTLAGLRARVGRDNTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPTGRDA
LGWNLGEALAPLGGKAQAFDNLDALVKAVVHTARPGDQILVMSNGGFGGVHQKLLDALSARGTS

Sequences:

>Translated_464_residues
MHIHILGICGTFMGGLAVLARGAGHTVTGCDAGVYPPMSTQLEAQGIGLIEGYDADQLNGLNADLFVIGNVVSRGNPLME
AILDRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTSSMLTWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEA
DEYDTAFFDKRSKFVHYRPKTAVLNNLEFDHADIFPDLAAIETQFHHLIRTVPGIGRIVTNGREDALERVLTRGCWSEVE
RFGVQGGWETLPAEDGVPVDERFAVYHNSERVGAVEWQVQGEHNRMNALAAIAAARHVGVPPAQAARSLASFRNVKRRME
VRGSVDGVTVYDDFAHHPTAIETTLAGLRARVGRDNTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPTGRDA
LGWNLGEALAPLGGKAQAFDNLDALVKAVVHTARPGDQILVMSNGGFGGVHQKLLDALSARGTS
>Mature_464_residues
MHIHILGICGTFMGGLAVLARGAGHTVTGCDAGVYPPMSTQLEAQGIGLIEGYDADQLNGLNADLFVIGNVVSRGNPLME
AILDRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTSSMLTWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEA
DEYDTAFFDKRSKFVHYRPKTAVLNNLEFDHADIFPDLAAIETQFHHLIRTVPGIGRIVTNGREDALERVLTRGCWSEVE
RFGVQGGWETLPAEDGVPVDERFAVYHNSERVGAVEWQVQGEHNRMNALAAIAAARHVGVPPAQAARSLASFRNVKRRME
VRGSVDGVTVYDDFAHHPTAIETTLAGLRARVGRDNTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPTGRDA
LGWNLGEALAPLGGKAQAFDNLDALVKAVVHTARPGDQILVMSNGGFGGVHQKLLDALSARGTS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=464, Percent_Identity=59.4827586206897, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1786279, Length=306, Percent_Identity=28.1045751633987, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49726; Mature: 49726

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLARGAGHTVTGCDAGVYPPMSTQLEAQGIGLIEGYDADQLNG
CEEEEEEHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCC
LNADLFVIGNVVSRGNPLMEAILDRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTSS
CCCEEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHH
MLTWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPK
HHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCC
TAVLNNLEFDHADIFPDLAAIETQFHHLIRTVPGIGRIVTNGREDALERVLTRGCWSEVE
HHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
RFGVQGGWETLPAEDGVPVDERFAVYHNSERVGAVEWQVQGEHNRMNALAAIAAARHVGV
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCC
PPAQAARSLASFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTLAGLRARVGRDNTRIL
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEE
AVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPTGRDALGWNLGEALAPLGGKAQAFD
EEECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHH
NLDALVKAVVHTARPGDQILVMSNGGFGGVHQKLLDALSARGTS
HHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLARGAGHTVTGCDAGVYPPMSTQLEAQGIGLIEGYDADQLNG
CEEEEEEHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCC
LNADLFVIGNVVSRGNPLMEAILDRGLPYTSGPQWLGEHVLNGKWVLAVAGTHGKTTTSS
CCCEEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHH
MLTWLLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPK
HHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCC
TAVLNNLEFDHADIFPDLAAIETQFHHLIRTVPGIGRIVTNGREDALERVLTRGCWSEVE
HHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
RFGVQGGWETLPAEDGVPVDERFAVYHNSERVGAVEWQVQGEHNRMNALAAIAAARHVGV
HHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCC
PPAQAARSLASFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTLAGLRARVGRDNTRIL
CHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEE
AVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPTGRDALGWNLGEALAPLGGKAQAFD
EEECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCHHHHH
NLDALVKAVVHTARPGDQILVMSNGGFGGVHQKLLDALSARGTS
HHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]