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The map label for this gene is gltJ [H]

Identifier: 91785181

GI number: 91785181

Start: 4214848

End: 4215588

Strand: Reverse

Name: gltJ [H]

Synonym: Bxe_A0598

Alternate gene names: 91785181

Gene position: 4215588-4214848 (Counterclockwise)

Preceding gene: 91785182

Following gene: 91785180

Centisome position: 86.11

GC content: 60.05

Gene sequence:

>741_bases
ATGTCATATCACTGGAACTGGGGCATTCTGCTGAGTCCCGTCTCCACCGGCGAGCCGACCACCTATCTGGGCTGGCTGCT
GTCGGGCTTCTGGGTGACGATTACCGTGTCGCTGTCGGCATGGGTGATCGCGCTGATCGTCGGGTCGTTGTTCGGCGTGT
TGCGCACGGTGCCGAATAAATGGGCGTCGGGTATCGGCACGCTCTATGTCGCGATTTTCCGAAACATTCCGCTGATCGTG
CAGTTCTTCATCTGGTATCTGGTGATACCGGAGTTGCTGCCCGTATCAATGGGCAACTGGTTCAAGCAACTGCCGCCGGG
CGCGCAGTTCTTTTCGTCGTCGATCATCTGTCTCGGGCTCTTCACTGCCGCGCGCGTGTGCGAGCAGGTGCGCTCGGGTA
TCAACGCACTGCCGCGCGGCCAGCGCGCCGCGGGTCTGGCAATGGGCTTCACGCAATGGCAGACGTACCGCTATGTGCTG
CTGCCGGTCGCATACCGGATCATCGTGCCGCCGCTCACGTCCGAGTTTCTGAATATTTTCAAGAACTCGGCGGTCGCGTC
GACGATCGGCCTGCTCGATCTGTCCGCTCAGGCGCGCCAACTGGTCGACTACACGTCGCAGACGTATGAGTCGTTTATCG
CGGTCACGGTGGCGTACATGCTGATCAATCTGGTCGTGATGCAACTCATGCGCTGGGTCGAAGCGAAGACCCGGTTGCCC
GGCTATATCGGAGGCAAGTAA

Upstream 100 bases:

>100_bases
GACAAGGCAATCGACTAAACCTTAGCTGTTTTTTTGAAACGCTGAACGAAACGGAAGGGGCCACGCGCTTCTTCCGTTTC
TTTTTGCTGGAGTCTTGGTC

Downstream 100 bases:

>100_bases
TGCACCATTTCGACTGGAGCGGTATTCCGGGCGCATTGCCTACGCTGTGGACCGGCGCCGTAGTCACTCTCAAGATCACG
CTGATCGCGATCGTGTTCGG

Product: His/Glu/Gln/Arg/opine ABC transporter inner membrane protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSYHWNWGILLSPVSTGEPTTYLGWLLSGFWVTITVSLSAWVIALIVGSLFGVLRTVPNKWASGIGTLYVAIFRNIPLIV
QFFIWYLVIPELLPVSMGNWFKQLPPGAQFFSSSIICLGLFTAARVCEQVRSGINALPRGQRAAGLAMGFTQWQTYRYVL
LPVAYRIIVPPLTSEFLNIFKNSAVASTIGLLDLSAQARQLVDYTSQTYESFIAVTVAYMLINLVVMQLMRWVEAKTRLP
GYIGGK

Sequences:

>Translated_246_residues
MSYHWNWGILLSPVSTGEPTTYLGWLLSGFWVTITVSLSAWVIALIVGSLFGVLRTVPNKWASGIGTLYVAIFRNIPLIV
QFFIWYLVIPELLPVSMGNWFKQLPPGAQFFSSSIICLGLFTAARVCEQVRSGINALPRGQRAAGLAMGFTQWQTYRYVL
LPVAYRIIVPPLTSEFLNIFKNSAVASTIGLLDLSAQARQLVDYTSQTYESFIAVTVAYMLINLVVMQLMRWVEAKTRLP
GYIGGK
>Mature_245_residues
SYHWNWGILLSPVSTGEPTTYLGWLLSGFWVTITVSLSAWVIALIVGSLFGVLRTVPNKWASGIGTLYVAIFRNIPLIVQ
FFIWYLVIPELLPVSMGNWFKQLPPGAQFFSSSIICLGLFTAARVCEQVRSGINALPRGQRAAGLAMGFTQWQTYRYVLL
PVAYRIIVPPLTSEFLNIFKNSAVASTIGLLDLSAQARQLVDYTSQTYESFIAVTVAYMLINLVVMQLMRWVEAKTRLPG
YIGGK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=247, Percent_Identity=64.7773279352227, Blast_Score=327, Evalue=4e-91,
Organism=Escherichia coli, GI1788226, Length=214, Percent_Identity=29.4392523364486, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786873, Length=216, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787030, Length=214, Percent_Identity=26.6355140186916, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI48994930, Length=116, Percent_Identity=38.7931034482759, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788645, Length=221, Percent_Identity=26.2443438914027, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787086, Length=221, Percent_Identity=25.3393665158371, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI87082239, Length=175, Percent_Identity=25.1428571428571, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788646, Length=219, Percent_Identity=27.8538812785388, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787087, Length=228, Percent_Identity=23.6842105263158, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27426; Mature: 27295

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCC
>Mature Secondary Structure 
SYHWNWGILLSPVSTGEPTTYLGWLLSGFWVTITVSLSAWVIALIVGSLFGVLRTVPNK
CEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WASGIGTLYVAIFRNIPLIVQFFIWYLVIPELLPVSMGNWFKQLPPGAQFFSSSIICLGL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FTAARVCEQVRSGINALPRGQRAAGLAMGFTQWQTYRYVLLPVAYRIIVPPLTSEFLNIF
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KNSAVASTIGLLDLSAQARQLVDYTSQTYESFIAVTVAYMLINLVVMQLMRWVEAKTRLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GYIGGK
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]