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The map label for this gene is citH [H]

Identifier: 91785135

GI number: 91785135

Start: 4169099

End: 4170463

Strand: Reverse

Name: citH [H]

Synonym: Bxe_A0645

Alternate gene names: 91785135

Gene position: 4170463-4169099 (Counterclockwise)

Preceding gene: 91785136

Following gene: 91785134

Centisome position: 85.18

GC content: 64.03

Gene sequence:

>1365_bases
ATGCAGACCCCCGCGTCGCCGTCGGCGACCACTTCCGCGCATTCGCCGTCTGCGCCGCTTTCCAAAGCGATGGTTCGGCG
GATCGTGTTTTCTTCGTCGGTCGGCAATGCGCTCGAATGGTTCGACTTTCTGGTCTACGGATACTTCGCGACCATCATCG
CCAAAGAGTTTTTCCCGATGCAGGACGAGTGGCTCTCCACGCTGCTCGCCATCGCCACCTTCGGCATTTCGTTCCTGATG
CGTCCGCTGGGCGCGGTCGTACTGGGCATCTATGGCGACCGCAAGGGCCGCAAGGCCGCACTGACGCTTGCTATCGCGCT
GATGATGGTCGGCACCTTCACGATGGCTGTCATGCCGCCATATGCAACCATCGGCATGGCGGCGCCGATCCTGATTCTGC
TCGCGCGGCTCGTGCAAGGCTTCGCGGTGGGCGGCGAGTTCGGCAGTGCGACGGCCTTCATGGTCGAACACAGCGCGTCG
CGCCGCGGCTACTACGCGAGCTGGCAGTTCGCGAGCCAGGGTCTCGCCGCGATCACGGCGGCCGCGTTCGGCTCGCTGCT
GACCGCCTGGCTGCCGCCCGCGCAACTGAATGACTGGGGCTGGCGCATGCCGTTCGTGTTCGGCTTGCTGGTCGGCCCGG
TGGGCTACTACATCCGTTCGCATCTCGACGAGACACCGGAATTCCTCGCGCTGCGCGAGGCGCGTGAGGCTGGCGGCGCA
CGCACGGCGGCAAGCGGCGAGAAGGACGCCTCGTTCGCCAGCCAGTGGGTCAACCTGCTGCTCGCGGTCGGTATCGTCGC
GCAATCCACGGTGGGTGTGTATGTGCTGCAACTGTACATGCCCATGTATGCGGTCAAGCAGTTGCACATGCCGGCGGCGG
CATCGTTCGGCGTGGTGGTGCTCAACGGCGGCCTGCAGTTCCTGCTTTCGCCTGTCATGGGCGCATGGTCCGACCGCATC
GGCCGGATTCGCATCATGCTGACCACGTCGATCCTGATGGGCGGGTTGATCTATCCGATGTTCGCACTGCTGCAGGCTCA
TCCGACCATTGGCTGGCTGCTGCTGTTGCAGGGCACGGCGGGAATTTTCAAGGCGGCGTATTCCGGGCCCATGCCCGCGT
TGATGTCCGAGATCTTTCCGACCCAGGTGCGCTCCACGGGTCTTTCAATCGGTTACAGCATCGGCGTGACGATCTTCGGC
GGCTTCGCGCCCACGATTGTCGAAACGTTCATTCATTTGACCGGCGACAAACTTGCGCCAAGTTACTACGTGCTGATTGC
CGCGGTACTGTCGGGCCTCTCGCTCGTCGTCGTGGCCTGGCGCATGCGCCGCGTGCGTCTGATGCAGGGCGTTCAGCCCG
CCTGA

Upstream 100 bases:

>100_bases
ATCGACTGCCGCTGCAAGGCATCGCACGAATCAGCTACACCGGACGCCAGCGGCGCTTCTGGCACACCCGGCATCGGGCA
TCAGACAGGCAGGAGATATC

Downstream 100 bases:

>100_bases
CCGGCAAGGCGGCCGGCGCCGAACGAGGCGCCGGCCGCCCTGCCTGGCATGATCGGCACGGCATCGTCGCAGACCTGAGC
CGGCTCTTCGAGCTGGCTTT

Product: major facilitator superfamily metabolite/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MQTPASPSATTSAHSPSAPLSKAMVRRIVFSSSVGNALEWFDFLVYGYFATIIAKEFFPMQDEWLSTLLAIATFGISFLM
RPLGAVVLGIYGDRKGRKAALTLAIALMMVGTFTMAVMPPYATIGMAAPILILLARLVQGFAVGGEFGSATAFMVEHSAS
RRGYYASWQFASQGLAAITAAAFGSLLTAWLPPAQLNDWGWRMPFVFGLLVGPVGYYIRSHLDETPEFLALREAREAGGA
RTAASGEKDASFASQWVNLLLAVGIVAQSTVGVYVLQLYMPMYAVKQLHMPAAASFGVVVLNGGLQFLLSPVMGAWSDRI
GRIRIMLTTSILMGGLIYPMFALLQAHPTIGWLLLLQGTAGIFKAAYSGPMPALMSEIFPTQVRSTGLSIGYSIGVTIFG
GFAPTIVETFIHLTGDKLAPSYYVLIAAVLSGLSLVVVAWRMRRVRLMQGVQPA

Sequences:

>Translated_454_residues
MQTPASPSATTSAHSPSAPLSKAMVRRIVFSSSVGNALEWFDFLVYGYFATIIAKEFFPMQDEWLSTLLAIATFGISFLM
RPLGAVVLGIYGDRKGRKAALTLAIALMMVGTFTMAVMPPYATIGMAAPILILLARLVQGFAVGGEFGSATAFMVEHSAS
RRGYYASWQFASQGLAAITAAAFGSLLTAWLPPAQLNDWGWRMPFVFGLLVGPVGYYIRSHLDETPEFLALREAREAGGA
RTAASGEKDASFASQWVNLLLAVGIVAQSTVGVYVLQLYMPMYAVKQLHMPAAASFGVVVLNGGLQFLLSPVMGAWSDRI
GRIRIMLTTSILMGGLIYPMFALLQAHPTIGWLLLLQGTAGIFKAAYSGPMPALMSEIFPTQVRSTGLSIGYSIGVTIFG
GFAPTIVETFIHLTGDKLAPSYYVLIAAVLSGLSLVVVAWRMRRVRLMQGVQPA
>Mature_454_residues
MQTPASPSATTSAHSPSAPLSKAMVRRIVFSSSVGNALEWFDFLVYGYFATIIAKEFFPMQDEWLSTLLAIATFGISFLM
RPLGAVVLGIYGDRKGRKAALTLAIALMMVGTFTMAVMPPYATIGMAAPILILLARLVQGFAVGGEFGSATAFMVEHSAS
RRGYYASWQFASQGLAAITAAAFGSLLTAWLPPAQLNDWGWRMPFVFGLLVGPVGYYIRSHLDETPEFLALREAREAGGA
RTAASGEKDASFASQWVNLLLAVGIVAQSTVGVYVLQLYMPMYAVKQLHMPAAASFGVVVLNGGLQFLLSPVMGAWSDRI
GRIRIMLTTSILMGGLIYPMFALLQAHPTIGWLLLLQGTAGIFKAAYSGPMPALMSEIFPTQVRSTGLSIGYSIGVTIFG
GFAPTIVETFIHLTGDKLAPSYYVLIAAVLSGLSLVVVAWRMRRVRLMQGVQPA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=443, Percent_Identity=33.8600451467269, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788292, Length=437, Percent_Identity=29.2906178489703, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1788942, Length=447, Percent_Identity=31.5436241610738, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=30.5687203791469, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87082231, Length=203, Percent_Identity=28.0788177339901, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87082404, Length=394, Percent_Identity=23.3502538071066, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48737; Mature: 48737

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPASPSATTSAHSPSAPLSKAMVRRIVFSSSVGNALEWFDFLVYGYFATIIAKEFFPM
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
QDEWLSTLLAIATFGISFLMRPLGAVVLGIYGDRKGRKAALTLAIALMMVGTFTMAVMPP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
YATIGMAAPILILLARLVQGFAVGGEFGSATAFMVEHSASRRGYYASWQFASQGLAAITA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCEEEHHHHHCCHHHHHH
AAFGSLLTAWLPPAQLNDWGWRMPFVFGLLVGPVGYYIRSHLDETPEFLALREAREAGGA
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
RTAASGEKDASFASQWVNLLLAVGIVAQSTVGVYVLQLYMPMYAVKQLHMPAAASFGVVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LNGGLQFLLSPVMGAWSDRIGRIRIMLTTSILMGGLIYPMFALLQAHPTIGWLLLLQGTA
ECCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCH
GIFKAAYSGPMPALMSEIFPTQVRSTGLSIGYSIGVTIFGGFAPTIVETFIHLTGDKLAP
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECHHEECCHHHHHHHHHHHHCCCCCCH
SYYVLIAAVLSGLSLVVVAWRMRRVRLMQGVQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQTPASPSATTSAHSPSAPLSKAMVRRIVFSSSVGNALEWFDFLVYGYFATIIAKEFFPM
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
QDEWLSTLLAIATFGISFLMRPLGAVVLGIYGDRKGRKAALTLAIALMMVGTFTMAVMPP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
YATIGMAAPILILLARLVQGFAVGGEFGSATAFMVEHSASRRGYYASWQFASQGLAAITA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEECCCCCCCCEEEHHHHHCCHHHHHH
AAFGSLLTAWLPPAQLNDWGWRMPFVFGLLVGPVGYYIRSHLDETPEFLALREAREAGGA
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCC
RTAASGEKDASFASQWVNLLLAVGIVAQSTVGVYVLQLYMPMYAVKQLHMPAAASFGVVV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
LNGGLQFLLSPVMGAWSDRIGRIRIMLTTSILMGGLIYPMFALLQAHPTIGWLLLLQGTA
ECCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEECCCH
GIFKAAYSGPMPALMSEIFPTQVRSTGLSIGYSIGVTIFGGFAPTIVETFIHLTGDKLAP
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEECHHEECCHHHHHHHHHHHHCCCCCCH
SYYVLIAAVLSGLSLVVVAWRMRRVRLMQGVQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]