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The map label for this gene is oprM [H]

Identifier: 91785070

GI number: 91785070

Start: 4096768

End: 4098315

Strand: Reverse

Name: oprM [H]

Synonym: Bxe_A0710

Alternate gene names: 91785070

Gene position: 4098315-4096768 (Counterclockwise)

Preceding gene: 91785071

Following gene: 91785068

Centisome position: 83.71

GC content: 64.92

Gene sequence:

>1548_bases
ATGCAAAAACACTCTCTGATTGCAGTGGCGGTCGCGCTGTTCGCGGCGGGTTGCACGATGGCGCCGAAATATGAGCGCCC
GGCCGCACCCGTCACGGCGACCTTCCCGACCGGCGGCGTGTACGACACGCAACCGGGCGCGACCCAGCCGGGCCAGCAGC
AGCCCGGCACGACAGGCGCCGCGCGCACCGCGAGCGGCCAGGCCGCGGCGGATATCGGCTGGCGCGATTTCTTCGTCGAT
CCGCGCATGCAGCGGTTGATCGAGATCGCGCTGAAGAACAACCGCGATCTGCGCGTGTCGGTGCTGAACATGCAGGCTTC
GGCCGCGCAGTTCCGGATCGTTCGCGCCGGCCTGCTGCCTACGCTCGACGCCTCGGCGTCGCAAACCAAGCAGCGCACGC
CTAAAGACCTGTCGTTCTTCGACCAGACGATTTCCAACTCGTACTCGGTCGGGCTGAACGCGTCGTGGGAAATCGACTTC
TTCGGGCGGATTCAGAGCCTGAAGGATCAGGCGTTGGCGCAGTACCTGGCCACGGCTCAGGCGCGCAAGGCGGCGGAAAT
CACGCTGGTCTCGCAGGTGGCGAACCAGTACATGACGGTGCTCGAACTCGACGACCTGCTGAAGGTCACCGAGAACACGT
TGAAGACCTCGCAGGAATCGTATCGGATCGCCAAGCTGCAGTTCGACAACGGTACGGGTTCGGAACTGGATCTGCGCCAG
TCGCAAACCGTGGTCGAGCAGGCCCTGGCCAACCTGCAGCAGCAGCAGCGGCTGCGGGCTCAGGCGGATAACGCGCTCGT
GCTGCTTCTCGGCGAGCCGTTGCCGGACGATCTGCCGCCGGGCATGACGCTGGACAACCAGAATCTGCTCACGGATATTC
CGGCGGGTCTGCCGTCCGATCTGCTGACGCGCCGGCCGGACATCATGGAGGCCGAAGAGAATCTGCTGGCCGCCAACGCG
AACATCGGCGCGGCGCGGGCCGCGTTCTTCCCGAAGGTTTCGCTGACCGGCAGCTTCGGTACGCTGAGCCCGTCGCTCGG
CGGGTTGTTCAAGCCGGGTTCGGCGGCGTGGAGCTTCGCGCCGTCGATCACGCTGCCGATCTTCGAAGGCGGGCAGAATC
TTGCCAACCTCGATCTCGCCAACATCCAGAAGAAAGTCCAGATCGCCACGTATGAAAAGGCGATCCAGACGGCCTTCCGC
GAAGTGGCAGACGCCCTGGCGGCACGCGGCACGTACGATCAGCAGATCCAGGCGCTGGAGCGCAATACTTTTGCGGAACA
ACGCCGGCTGGATCTGTCGAATCTGCGCTATACGAATGGCGTCGATAGTTATCTGTCGGTGCTGACCGCGCAGACGGATC
TGTATTCGTCGCAGCAGTTGCTGGTCACGGCCCGGATGGAACGTCTGCAGAACCTCGTGACGTTGTATCAGGCACTGGGC
GGCGGCTGGGTCGAGCACAACGGCGATCAGCCGCGGCCGGCCGACGCGCCGGTCGACTACGGTGCGGCGAGTGCGCCGGT
GGCGGCTTCGGCCGCTACGGCGGGTTGA

Upstream 100 bases:

>100_bases
GACCCGGATGAGGCGCTCGCACACTATAACCAGCACCATCCGCATGACGGCCAGGGCGGCGGGGCGGCTGATGAAGGCTC
GGGCAAGGACGGACACTGAG

Downstream 100 bases:

>100_bases
GTCCTGTAGCTTCAGGCGCGCAAGGCGGGTTTCTGTTTCAGTAAAACCGTTCTGCGGGGCCAGTCACATGAAAAACGCCA
CGGCATGCCGTGGCGTTTTT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MQKHSLIAVAVALFAAGCTMAPKYERPAAPVTATFPTGGVYDTQPGATQPGQQQPGTTGAARTASGQAAADIGWRDFFVD
PRMQRLIEIALKNNRDLRVSVLNMQASAAQFRIVRAGLLPTLDASASQTKQRTPKDLSFFDQTISNSYSVGLNASWEIDF
FGRIQSLKDQALAQYLATAQARKAAEITLVSQVANQYMTVLELDDLLKVTENTLKTSQESYRIAKLQFDNGTGSELDLRQ
SQTVVEQALANLQQQQRLRAQADNALVLLLGEPLPDDLPPGMTLDNQNLLTDIPAGLPSDLLTRRPDIMEAEENLLAANA
NIGAARAAFFPKVSLTGSFGTLSPSLGGLFKPGSAAWSFAPSITLPIFEGGQNLANLDLANIQKKVQIATYEKAIQTAFR
EVADALAARGTYDQQIQALERNTFAEQRRLDLSNLRYTNGVDSYLSVLTAQTDLYSSQQLLVTARMERLQNLVTLYQALG
GGWVEHNGDQPRPADAPVDYGAASAPVAASAATAG

Sequences:

>Translated_515_residues
MQKHSLIAVAVALFAAGCTMAPKYERPAAPVTATFPTGGVYDTQPGATQPGQQQPGTTGAARTASGQAAADIGWRDFFVD
PRMQRLIEIALKNNRDLRVSVLNMQASAAQFRIVRAGLLPTLDASASQTKQRTPKDLSFFDQTISNSYSVGLNASWEIDF
FGRIQSLKDQALAQYLATAQARKAAEITLVSQVANQYMTVLELDDLLKVTENTLKTSQESYRIAKLQFDNGTGSELDLRQ
SQTVVEQALANLQQQQRLRAQADNALVLLLGEPLPDDLPPGMTLDNQNLLTDIPAGLPSDLLTRRPDIMEAEENLLAANA
NIGAARAAFFPKVSLTGSFGTLSPSLGGLFKPGSAAWSFAPSITLPIFEGGQNLANLDLANIQKKVQIATYEKAIQTAFR
EVADALAARGTYDQQIQALERNTFAEQRRLDLSNLRYTNGVDSYLSVLTAQTDLYSSQQLLVTARMERLQNLVTLYQALG
GGWVEHNGDQPRPADAPVDYGAASAPVAASAATAG
>Mature_515_residues
MQKHSLIAVAVALFAAGCTMAPKYERPAAPVTATFPTGGVYDTQPGATQPGQQQPGTTGAARTASGQAAADIGWRDFFVD
PRMQRLIEIALKNNRDLRVSVLNMQASAAQFRIVRAGLLPTLDASASQTKQRTPKDLSFFDQTISNSYSVGLNASWEIDF
FGRIQSLKDQALAQYLATAQARKAAEITLVSQVANQYMTVLELDDLLKVTENTLKTSQESYRIAKLQFDNGTGSELDLRQ
SQTVVEQALANLQQQQRLRAQADNALVLLLGEPLPDDLPPGMTLDNQNLLTDIPAGLPSDLLTRRPDIMEAEENLLAANA
NIGAARAAFFPKVSLTGSFGTLSPSLGGLFKPGSAAWSFAPSITLPIFEGGQNLANLDLANIQKKVQIATYEKAIQTAFR
EVADALAARGTYDQQIQALERNTFAEQRRLDLSNLRYTNGVDSYLSVLTAQTDLYSSQQLLVTARMERLQNLVTLYQALG
GGWVEHNGDQPRPADAPVDYGAASAPVAASAATAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=483, Percent_Identity=40.1656314699793, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI2367348, Length=434, Percent_Identity=24.8847926267281, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55492; Mature: 55492

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKHSLIAVAVALFAAGCTMAPKYERPAAPVTATFPTGGVYDTQPGATQPGQQQPGTTGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
ARTASGQAAADIGWRDFFVDPRMQRLIEIALKNNRDLRVSVLNMQASAAQFRIVRAGLLP
CCCCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCC
TLDASASQTKQRTPKDLSFFDQTISNSYSVGLNASWEIDFFGRIQSLKDQALAQYLATAQ
CCCCCHHHHHHCCCCHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ARKAAEITLVSQVANQYMTVLELDDLLKVTENTLKTSQESYRIAKLQFDNGTGSELDLRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCCCCCHHH
SQTVVEQALANLQQQQRLRAQADNALVLLLGEPLPDDLPPGMTLDNQNLLTDIPAGLPSD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHCCCCCCHH
LLTRRPDIMEAEENLLAANANIGAARAAFFPKVSLTGSFGTLSPSLGGLFKPGSAAWSFA
HHHCCCCHHHHHHHHEEECCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEECC
PSITLPIFEGGQNLANLDLANIQKKVQIATYEKAIQTAFREVADALAARGTYDQQIQALE
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RNTFAEQRRLDLSNLRYTNGVDSYLSVLTAQTDLYSSQQLLVTARMERLQNLVTLYQALG
HHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GGWVEHNGDQPRPADAPVDYGAASAPVAASAATAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKHSLIAVAVALFAAGCTMAPKYERPAAPVTATFPTGGVYDTQPGATQPGQQQPGTTGA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
ARTASGQAAADIGWRDFFVDPRMQRLIEIALKNNRDLRVSVLNMQASAAQFRIVRAGLLP
CCCCCCCCHHCCCCHHHCCCHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHCCCC
TLDASASQTKQRTPKDLSFFDQTISNSYSVGLNASWEIDFFGRIQSLKDQALAQYLATAQ
CCCCCHHHHHHCCCCHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
ARKAAEITLVSQVANQYMTVLELDDLLKVTENTLKTSQESYRIAKLQFDNGTGSELDLRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCCCCCHHH
SQTVVEQALANLQQQQRLRAQADNALVLLLGEPLPDDLPPGMTLDNQNLLTDIPAGLPSD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEECCCCHHHHCCCCCCHH
LLTRRPDIMEAEENLLAANANIGAARAAFFPKVSLTGSFGTLSPSLGGLFKPGSAAWSFA
HHHCCCCHHHHHHHHEEECCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEECC
PSITLPIFEGGQNLANLDLANIQKKVQIATYEKAIQTAFREVADALAARGTYDQQIQALE
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RNTFAEQRRLDLSNLRYTNGVDSYLSVLTAQTDLYSSQQLLVTARMERLQNLVTLYQALG
HHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GGWVEHNGDQPRPADAPVDYGAASAPVAASAATAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]