Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 91784957

GI number: 91784957

Start: 3967310

End: 3968479

Strand: Reverse

Name: braE [H]

Synonym: Bxe_A0823

Alternate gene names: 91784957

Gene position: 3968479-3967310 (Counterclockwise)

Preceding gene: 91784958

Following gene: 91784956

Centisome position: 81.06

GC content: 62.82

Gene sequence:

>1170_bases
ATGACCTCAATTCAACCGATCGAACCGTCCACGACGCTCATCCCTGAAAAGAACCTGACCAGGACGCTGATGGTCGGCAT
CATCACCGCGATCTTCGTGATCGCGGCGCCGATGATCATCGGCGCGGCAGGCGGCAACTACTGGGTCCGCGTGCTCGACT
TCGCGATGCTCTACGTGATGCTCGCGCTCGGCCTGAACGTGGTGGTCGGCTTCGCCGGCCTGCTCGACCTGGGCTACATC
GCGTTTTACGCGGTCGGCGCCTACGTTGCGGCGCTGCTGAGTTCGCCGCACCTGTCCACGCAGTTCGAGTGGATCGCGCA
TCTCGCGCCGAACGGGCTGCACGTGCCGTTCCTGATCATCGTGCCGTGCGCGATGGCTCTCGCCGCGCTGTTCGGCGTGC
TGCTCGGCGCACCGACGCTGCGTCTGCGTGGCGATTACCTCGCCATCGTGACGTTGGGCTTCGGGGAAATCGTGCGGATC
TTCATGAACAACCTCGACCGTCCGGTGAACATCACCAACGGCCCGAAGGGGATCACGGCGATCGACCCGGTGCATTTCGG
CGACTTCAGCCTCGCGCAGACGCACTCGCTGTTCGGGTTGCAGTTCCCGTCGGTCTACTCGTACTACTACCTGTTCGTGA
TCGCCGCCCTGGTCGTGATCTGGGTGTGTACGCGTTTGCAGCACTCGCGTATCGGCCGTGCATGGGCCGCGATCCGCGAG
GACGAAATCGCCGCCAAGGCAATGGGCATCAACACCCGCAACGTGAAATTGCTGGCGTTCGCGATGGGCGCTTCGTTCGG
CGGTCTGTCGGGCGCGATGTTCGGCTCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACGCTGCCGGAATCGATCGTGG
TGCTGGCGTGCGTGGTGCTGGGCGGCATGGGCCATATTCCGGGCGTGATTCTCGGCGCGGTGCTGCTCGCCGTGCTGCCG
GAATTCCTGCGCTCGACGATGGGCCCGCTGCAGAACATGATCTTCGGCCATGAAATCGTCGATACGGAAGTGATCCGTCA
GCTGGTCTATGCACTCGCGATGGTGCTGATCATGCTGTACCGCTCGGAAGGCTTGTGGCCGTCGCCCAAGCATGAAGACA
AGATTGCGAAACTGTCGAAGCGCAACGGCAAGAAGCCGGTGCGGGTCTAA

Upstream 100 bases:

>100_bases
GTTCGCCTTTATCGTGCTGATCATCGTGCTGGTGTTCCGTCCGTCGGGTCTGCTCGGCGAACGCGTGGCGGATCGCGCCT
GATCCTGGGAGAAAGCAAAC

Downstream 100 bases:

>100_bases
CGGACATGGAGAATAAATATGAGCGATAACATTCGACTGTCCGTCAAAGGCGTGAACAAACGCTTCGGCGGTTTGCAGGC
GCTGTCCGATGTCGGTCTTG

Product: branched chain amino acid ABC transporter inner membrane protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIEPSTTLIPEKNLTRTLMVGIITAIFVIAAPMIIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYVAALLSSPHLSTQFEWIAHLAPNGLHVPFLIIVPCAMALAALFGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITAIDPVHFGDFSLAQTHSLFGLQFPSVYSYYYLFVIAALVVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTLPESIVVLACVVLGGMGHIPGVILGAVLLAVLP
EFLRSTMGPLQNMIFGHEIVDTEVIRQLVYALAMVLIMLYRSEGLWPSPKHEDKIAKLSKRNGKKPVRV

Sequences:

>Translated_389_residues
MTSIQPIEPSTTLIPEKNLTRTLMVGIITAIFVIAAPMIIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYVAALLSSPHLSTQFEWIAHLAPNGLHVPFLIIVPCAMALAALFGVLLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITAIDPVHFGDFSLAQTHSLFGLQFPSVYSYYYLFVIAALVVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTLPESIVVLACVVLGGMGHIPGVILGAVLLAVLP
EFLRSTMGPLQNMIFGHEIVDTEVIRQLVYALAMVLIMLYRSEGLWPSPKHEDKIAKLSKRNGKKPVRV
>Mature_388_residues
TSIQPIEPSTTLIPEKNLTRTLMVGIITAIFVIAAPMIIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYVAALLSSPHLSTQFEWIAHLAPNGLHVPFLIIVPCAMALAALFGVLLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPVNITNGPKGITAIDPVHFGDFSLAQTHSLFGLQFPSVYSYYYLFVIAALVVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTLPESIVVLACVVLGGMGHIPGVILGAVLLAVLPE
FLRSTMGPLQNMIFGHEIVDTEVIRQLVYALAMVLIMLYRSEGLWPSPKHEDKIAKLSKRNGKKPVRV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=341, Percent_Identity=42.5219941348974, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 42075; Mature: 41943

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSIQPIEPSTTLIPEKNLTRTLMVGIITAIFVIAAPMIIGAAGGNYWVRVLDFAMLYVM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYVAALLSSPHLSTQFEWIAHLAPNGLHVPFLII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHH
VPCAMALAALFGVLLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITA
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCE
IDPVHFGDFSLAQTHSLFGLQFPSVYSYYYLFVIAALVVIWVCTRLQHSRIGRAWAAIRE
ECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGSFQGFVSPESFTLPESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVLPEFLRSTMGPLQNMIFGHEIVDTEVIRQLVYALAMVLIMLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RSEGLWPSPKHEDKIAKLSKRNGKKPVRV
HCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]