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The map label for this gene is iaaA [H]

Identifier: 91784946

GI number: 91784946

Start: 3958075

End: 3959067

Strand: Reverse

Name: iaaA [H]

Synonym: Bxe_A0834

Alternate gene names: 91784946

Gene position: 3959067-3958075 (Counterclockwise)

Preceding gene: 91784947

Following gene: 91784945

Centisome position: 80.87

GC content: 68.58

Gene sequence:

>993_bases
ATGAACTCCAACGCAGTCATTGCCATTCATGGCGGAGCAGGGACGATCCTGCGCGCGTCGATGTCGGCCAGCGCCGAAGC
CGACTATCACGCCGCCTTGCATGCGGTGCTGAGCGCCGGCCAGCGCGTGCTTGCCGAGGGCGGCAGCGCACTCGACGCCG
TCAGCGAGGCCGTGCGCCTGCTCGAAGACTGCCCGCTCTTCAACGCGGGCCGCGGCGCGGTTTTTACGGCGGCGGGCACG
CACGAACTCGACGCCGCGATCATGGACGGCCGTACGCTCGAAGCCGGCGCGATCTGCTGCGTGAAGCGCGTGCGCAATCC
GGTTCTGGCCGCGCGCCGCGTGCTCGAAGGCAGCGAGCATGTGCTGTTCACGGGCGAAGGTGCGGAAGCGTTCGCCGCCG
CGCAAGGGCTCGAATTGGTCGAGCCTGAGTATTTCCATACCGAAGCGCGCCATCGTCAATGGCTGCTCGCGCGCGATCAG
CAGCGCGCCATGCTCGATCACGACGGCGCCACGCTCGCCGCCGCGCCGGCTTCGAACGACGACGATCCCACGCCGCATGA
ACCAATCGATCCGAACCGCAAGTTCGGCACGGTCGGCGCGGTCGCGCTCGATCGGCACGGCCACGTGGCGGCGGCGACCT
CGACGGGCGGCGTCACCAACAAGCAGGTCGGCCGGGTCGGCGATGCGCCGCTGATCGGCGCCGGCTGCTATGCGGACGAC
GCGACCTGCGCCGTCTCGACGACCGGTTCGGGCGAGATGTTCATGCGCATGGTGGCGGCCTACGACGTCGCCGCGCAAAT
GGCCTACCGCAACGTGTCGCTGCAGGAAGCGGCCGACGACGTGGTGATGAACCGCCTTCCGAAGATCGACGGCCGCGGCG
GCCTGATCGCCGTCGATGCGCGCGGCAACGTCGCGCTGCCGTTCAATACCGAAGGCATGTATCGCGGTTTTGCGCGGCTC
GGTGAAACGCCGGTGACGGCGATTTACCGCTAG

Upstream 100 bases:

>100_bases
ATCTGCAAGCGGGAGCCGCCGGCACGAGCGCGCGGCCCGCGCGCAACGCGACCCGAACCACCCGTACTGCCCGAACCACT
CGCACTAGCCAATCCAAACG

Downstream 100 bases:

>100_bases
TCGTTCTGCTCGCCGATAGCGTGAATCTTCGTTTCAAACTCGCCTGAATTTCAGTTAGCCGCGTTCGAATTCAAAGGAAC
CACCGTGCCGACTCCATCGC

Product: peptidase T2, asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNSNAVIAIHGGAGTILRASMSASAEADYHAALHAVLSAGQRVLAEGGSALDAVSEAVRLLEDCPLFNAGRGAVFTAAGT
HELDAAIMDGRTLEAGAICCVKRVRNPVLAARRVLEGSEHVLFTGEGAEAFAAAQGLELVEPEYFHTEARHRQWLLARDQ
QRAMLDHDGATLAAAPASNDDDPTPHEPIDPNRKFGTVGAVALDRHGHVAAATSTGGVTNKQVGRVGDAPLIGAGCYADD
ATCAVSTTGSGEMFMRMVAAYDVAAQMAYRNVSLQEAADDVVMNRLPKIDGRGGLIAVDARGNVALPFNTEGMYRGFARL
GETPVTAIYR

Sequences:

>Translated_330_residues
MNSNAVIAIHGGAGTILRASMSASAEADYHAALHAVLSAGQRVLAEGGSALDAVSEAVRLLEDCPLFNAGRGAVFTAAGT
HELDAAIMDGRTLEAGAICCVKRVRNPVLAARRVLEGSEHVLFTGEGAEAFAAAQGLELVEPEYFHTEARHRQWLLARDQ
QRAMLDHDGATLAAAPASNDDDPTPHEPIDPNRKFGTVGAVALDRHGHVAAATSTGGVTNKQVGRVGDAPLIGAGCYADD
ATCAVSTTGSGEMFMRMVAAYDVAAQMAYRNVSLQEAADDVVMNRLPKIDGRGGLIAVDARGNVALPFNTEGMYRGFARL
GETPVTAIYR
>Mature_330_residues
MNSNAVIAIHGGAGTILRASMSASAEADYHAALHAVLSAGQRVLAEGGSALDAVSEAVRLLEDCPLFNAGRGAVFTAAGT
HELDAAIMDGRTLEAGAICCVKRVRNPVLAARRVLEGSEHVLFTGEGAEAFAAAQGLELVEPEYFHTEARHRQWLLARDQ
QRAMLDHDGATLAAAPASNDDDPTPHEPIDPNRKFGTVGAVALDRHGHVAAATSTGGVTNKQVGRVGDAPLIGAGCYADD
ATCAVSTTGSGEMFMRMVAAYDVAAQMAYRNVSLQEAADDVVMNRLPKIDGRGGLIAVDARGNVALPFNTEGMYRGFARL
GETPVTAIYR

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=320, Percent_Identity=37.8125, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI145275200, Length=320, Percent_Identity=37.8125, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI285002251, Length=237, Percent_Identity=36.7088607594937, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI98991769, Length=279, Percent_Identity=33.6917562724014, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI285002253, Length=237, Percent_Identity=34.5991561181435, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1787050, Length=327, Percent_Identity=59.0214067278288, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=37.3831775700935, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21357893, Length=271, Percent_Identity=33.210332103321, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221330102, Length=241, Percent_Identity=33.6099585062241, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652135, Length=250, Percent_Identity=33.2, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24655553, Length=222, Percent_Identity=31.0810810810811, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24657358, Length=237, Percent_Identity=29.535864978903, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 34491; Mature: 34491

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNAVIAIHGGAGTILRASMSASAEADYHAALHAVLSAGQRVLAEGGSALDAVSEAVRL
CCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHH
LEDCPLFNAGRGAVFTAAGTHELDAAIMDGRTLEAGAICCVKRVRNPVLAARRVLEGSEH
HHHCCCCCCCCCEEEEECCCCHHHHHHHCCCEECHHHHHHHHHHCCHHHHHHHHHCCCCE
VLFTGEGAEAFAAAQGLELVEPEYFHTEARHRQWLLARDQQRAMLDHDGATLAAAPASND
EEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCC
DDPTPHEPIDPNRKFGTVGAVALDRHGHVAAATSTGGVTNKQVGRVGDAPLIGAGCYADD
CCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCHHCCCCCCCEEECCCCCCC
ATCAVSTTGSGEMFMRMVAAYDVAAQMAYRNVSLQEAADDVVMNRLPKIDGRGGLIAVDA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
RGNVALPFNTEGMYRGFARLGETPVTAIYR
CCCEEEEECCCHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MNSNAVIAIHGGAGTILRASMSASAEADYHAALHAVLSAGQRVLAEGGSALDAVSEAVRL
CCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHH
LEDCPLFNAGRGAVFTAAGTHELDAAIMDGRTLEAGAICCVKRVRNPVLAARRVLEGSEH
HHHCCCCCCCCCEEEEECCCCHHHHHHHCCCEECHHHHHHHHHHCCHHHHHHHHHCCCCE
VLFTGEGAEAFAAAQGLELVEPEYFHTEARHRQWLLARDQQRAMLDHDGATLAAAPASND
EEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCC
DDPTPHEPIDPNRKFGTVGAVALDRHGHVAAATSTGGVTNKQVGRVGDAPLIGAGCYADD
CCCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCHHCCCCCCCEEECCCCCCC
ATCAVSTTGSGEMFMRMVAAYDVAAQMAYRNVSLQEAADDVVMNRLPKIDGRGGLIAVDA
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
RGNVALPFNTEGMYRGFARLGETPVTAIYR
CCCEEEEECCCHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]