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The map label for this gene is pcl [H]

Identifier: 91784861

GI number: 91784861

Start: 3862508

End: 3864355

Strand: Reverse

Name: pcl [H]

Synonym: Bxe_A0931

Alternate gene names: 91784861

Gene position: 3864355-3862508 (Counterclockwise)

Preceding gene: 91784862

Following gene: 91784860

Centisome position: 78.93

GC content: 67.91

Gene sequence:

>1848_bases
ATGATCGCGCTGCATGATCTGTTGTCGGTGGTTCATGAGAGCGCCGCGCTGCGCACACCCGTTTGCCGGGAGGGTGGCAC
GGTGCTCGATCGCGCGGCGTTTCGCGCGCGCGTCGCCGCCTTGATCGCGCTCGTGCAAACGCGGGCCGCGCAACGCTACG
CACTCTGTATCGACGATCCGTTCGATTTCGCCTGCGCGCTGTTCGCATTGTTCGCGTGCGGCAAGGAGCCGGTGATTCCG
GCCAATGCGACGCCGGGCTATCTGGCCGATCTCGCGGATGCTTATGACGCGGTGTTGACCGATGCGGATTTGCCGCCGTT
CGAGTTTGCTGCTAAAGCCAACGTTAGTGTCGACGCCGAAGCTGAAGTCAACGCAGTCATCCATGCCGATACCGGCCTCG
ACACGGGTGTCGAAGTCGGCGTCAGCGCAGCAGCGGCAGGCAACGCAGGAGTCGACACCGATACTGGAAACGCCGAGGCA
GCCTCGGCGTACGCATCGAACGCCGCTTACCCGATCGCCCGCCACGCCCCGCTTACGCTTTACACATCCGGCAGCAGCGG
CCGCCCCAAGCCGATCCGCAAAACGCTCGCGCAATTCAACGCCGAAGTGCACACACTGGAAAAACAATGGGGCGCGCTGG
TCGGCGACGCGACGATGCTCGCGAGCGTGCCGCATCACCACATCTACGGTTTGCTGTTTCGCGTGCTGTGGCCGCTTGCC
GCCGGCCGTGCCTTCGACCGTGCGGTCAGCATCGAGCCATTGCATCTGCAAGCGCAGATCGAACAATGCGGCGCGGTGGT
GATCGTCTCGACGCCCGCGCAACTGTCGCGCTGGCCCGCGTTGCCCGGCTTTGCCGACCTGACGCCGGCGCCGCGCGCGT
TCTTTTCGTCGGGCGGCCCGCTGGCGCCTGAAGCCGCGCAGGAATACGCCGCAGCCTATGGCGCGGCGCCGCTCGAAATT
TACGGCAGCACGGAAACCGGCGGCATCGCATGGCGGCGCCAGGATCAGAGCGACGCCTGGCAACCGGTGGCCGGAATCGA
CGTGCGCCGCGACGAAGACGGCGCGCTGAACGTCCGCTCGCCGCACCTCGATCACACGGGCTGGCATCGCACCGACGACA
GGATCGCTTTCGACGCCGACGGCCGTTTCCGCCTGCAAGGCCGGCTCGACCGCGTGCTCAAGCTCGACGGCAAACGCGTG
TCGTTGCCGGAACTGGAAGCGCGCCTCGCGCTGCATCCGTATGTGGCGCAAGCGGCGATCGTGCCGCTGACGGGCGCCTC
GCGCGAGCGCGTCGGCGCCGTCGTGGCGCTCACCGAAGCGGGCGGCGCGGCATTGCGCGACGAAGGCCGCGTGGCGCTCG
CAAAAATGTTGCGCCGGCACCTCGCCGAATATTTCGACGTCGTGGTGCTGCCGCGCCATTGGCGTTTTCGCGTCACGCTG
CCGTTCGACGCGCGCGGCAAACTGCCGGTGGCCGCCGTGGCGGCCGCGTTCGAGCCGCGCAGCGAAGGTGTCGAAGTGCT
GGCCGAAGCGCGCAGCGGCGATACGCTGCATTACGAGTTGCGCGTGCCGCCCACGCTCGTGCATTTCGCCGGGCATTTTC
CCGGCCTGCCGATTCTGCCCGGCGTCGTTCAGATCGACTGGGCGATGCGCCTCGCCGCCGACCACTTGCCGGCCGTGCGC
GCGCTGGCGTCGATCGATCGTCTGAAGTTCATGGCGCCGGTGCCGCCGGGCGCCGTGCTCAAGCTCACGCTCGCTCACGA
CGCGGCGCGCCGGCGCGTGCAGTTCGCGTATCGCGTGAACGGCCGCGATTGCGCGTCCGGTGTGATCGTCTACGGGGCGC
CGGCGTGA

Upstream 100 bases:

>100_bases
ACGGCGCGATCGCTTATGGGCTGATCGGCGTGCTGCTGGCGGCCGAAGTCGTCTGGCGTTATCTGGTCGTGCTGCCGCGC
GCCGCGCGTTCGGAGGCGGC

Downstream 100 bases:

>100_bases
AGACCTTCTCCCCATGCATCGTCATTCCGATCTACAACCATAAGGACGCGATCAGCGCGACCGTCGCTCATCTCGCGGTT
CACGGCCTGCCGTTTTTCGT

Product: putative AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: 4CL; 4-coumaroyl-CoA synthase [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MIALHDLLSVVHESAALRTPVCREGGTVLDRAAFRARVAALIALVQTRAAQRYALCIDDPFDFACALFALFACGKEPVIP
ANATPGYLADLADAYDAVLTDADLPPFEFAAKANVSVDAEAEVNAVIHADTGLDTGVEVGVSAAAAGNAGVDTDTGNAEA
ASAYASNAAYPIARHAPLTLYTSGSSGRPKPIRKTLAQFNAEVHTLEKQWGALVGDATMLASVPHHHIYGLLFRVLWPLA
AGRAFDRAVSIEPLHLQAQIEQCGAVVIVSTPAQLSRWPALPGFADLTPAPRAFFSSGGPLAPEAAQEYAAAYGAAPLEI
YGSTETGGIAWRRQDQSDAWQPVAGIDVRRDEDGALNVRSPHLDHTGWHRTDDRIAFDADGRFRLQGRLDRVLKLDGKRV
SLPELEARLALHPYVAQAAIVPLTGASRERVGAVVALTEAGGAALRDEGRVALAKMLRRHLAEYFDVVVLPRHWRFRVTL
PFDARGKLPVAAVAAAFEPRSEGVEVLAEARSGDTLHYELRVPPTLVHFAGHFPGLPILPGVVQIDWAMRLAADHLPAVR
ALASIDRLKFMAPVPPGAVLKLTLAHDAARRRVQFAYRVNGRDCASGVIVYGAPA

Sequences:

>Translated_615_residues
MIALHDLLSVVHESAALRTPVCREGGTVLDRAAFRARVAALIALVQTRAAQRYALCIDDPFDFACALFALFACGKEPVIP
ANATPGYLADLADAYDAVLTDADLPPFEFAAKANVSVDAEAEVNAVIHADTGLDTGVEVGVSAAAAGNAGVDTDTGNAEA
ASAYASNAAYPIARHAPLTLYTSGSSGRPKPIRKTLAQFNAEVHTLEKQWGALVGDATMLASVPHHHIYGLLFRVLWPLA
AGRAFDRAVSIEPLHLQAQIEQCGAVVIVSTPAQLSRWPALPGFADLTPAPRAFFSSGGPLAPEAAQEYAAAYGAAPLEI
YGSTETGGIAWRRQDQSDAWQPVAGIDVRRDEDGALNVRSPHLDHTGWHRTDDRIAFDADGRFRLQGRLDRVLKLDGKRV
SLPELEARLALHPYVAQAAIVPLTGASRERVGAVVALTEAGGAALRDEGRVALAKMLRRHLAEYFDVVVLPRHWRFRVTL
PFDARGKLPVAAVAAAFEPRSEGVEVLAEARSGDTLHYELRVPPTLVHFAGHFPGLPILPGVVQIDWAMRLAADHLPAVR
ALASIDRLKFMAPVPPGAVLKLTLAHDAARRRVQFAYRVNGRDCASGVIVYGAPA
>Mature_615_residues
MIALHDLLSVVHESAALRTPVCREGGTVLDRAAFRARVAALIALVQTRAAQRYALCIDDPFDFACALFALFACGKEPVIP
ANATPGYLADLADAYDAVLTDADLPPFEFAAKANVSVDAEAEVNAVIHADTGLDTGVEVGVSAAAAGNAGVDTDTGNAEA
ASAYASNAAYPIARHAPLTLYTSGSSGRPKPIRKTLAQFNAEVHTLEKQWGALVGDATMLASVPHHHIYGLLFRVLWPLA
AGRAFDRAVSIEPLHLQAQIEQCGAVVIVSTPAQLSRWPALPGFADLTPAPRAFFSSGGPLAPEAAQEYAAAYGAAPLEI
YGSTETGGIAWRRQDQSDAWQPVAGIDVRRDEDGALNVRSPHLDHTGWHRTDDRIAFDADGRFRLQGRLDRVLKLDGKRV
SLPELEARLALHPYVAQAAIVPLTGASRERVGAVVALTEAGGAALRDEGRVALAKMLRRHLAEYFDVVVLPRHWRFRVTL
PFDARGKLPVAAVAAAFEPRSEGVEVLAEARSGDTLHYELRVPPTLVHFAGHFPGLPILPGVVQIDWAMRLAADHLPAVR
ALASIDRLKFMAPVPPGAVLKLTLAHDAARRRVQFAYRVNGRDCASGVIVYGAPA

Specific function: Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32563687, Length=297, Percent_Identity=23.9057239057239, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=317, Percent_Identity=24.6056782334385, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012743
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.12 [H]

Molecular weight: Translated: 65689; Mature: 65689

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALHDLLSVVHESAALRTPVCREGGTVLDRAAFRARVAALIALVQTRAAQRYALCIDDP
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
FDFACALFALFACGKEPVIPANATPGYLADLADAYDAVLTDADLPPFEFAAKANVSVDAE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEECCC
AEVNAVIHADTGLDTGVEVGVSAAAAGNAGVDTDTGNAEAASAYASNAAYPIARHAPLTL
CCEEEEEEECCCCCCCHHHCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEECCCCEEE
YTSGSSGRPKPIRKTLAQFNAEVHTLEKQWGALVGDATMLASVPHHHIYGLLFRVLWPLA
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHCHHHCCHHHHHHCCHHHHHHHHHHHHHHHH
AGRAFDRAVSIEPLHLQAQIEQCGAVVIVSTPAQLSRWPALPGFADLTPAPRAFFSSGGP
CCCHHHHEECCCCEEHHEEHHHCCEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHCCCCC
LAPEAAQEYAAAYGAAPLEIYGSTETGGIAWRRQDQSDAWQPVAGIDVRRDEDGALNVRS
CCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEEEECC
PHLDHTGWHRTDDRIAFDADGRFRLQGRLDRVLKLDGKRVSLPELEARLALHPYVAQAAI
CCCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHCEEE
VPLTGASRERVGAVVALTEAGGAALRDEGRVALAKMLRRHLAEYFDVVVLPRHWRFRVTL
EECCCCCHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCEEEEEEE
PFDARGKLPVAAVAAAFEPRSEGVEVLAEARSGDTLHYELRVPPTLVHFAGHFPGLPILP
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCHHHHHHCCCCCCCCCCC
GVVQIDWAMRLAADHLPAVRALASIDRLKFMAPVPPGAVLKLTLAHDAARRRVQFAYRVN
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEHHHHHHHHEEEEEEEC
GRDCASGVIVYGAPA
CCCCCCCEEEEECCC
>Mature Secondary Structure
MIALHDLLSVVHESAALRTPVCREGGTVLDRAAFRARVAALIALVQTRAAQRYALCIDDP
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
FDFACALFALFACGKEPVIPANATPGYLADLADAYDAVLTDADLPPFEFAAKANVSVDAE
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEECCC
AEVNAVIHADTGLDTGVEVGVSAAAAGNAGVDTDTGNAEAASAYASNAAYPIARHAPLTL
CCEEEEEEECCCCCCCHHHCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCCEECCCCEEE
YTSGSSGRPKPIRKTLAQFNAEVHTLEKQWGALVGDATMLASVPHHHIYGLLFRVLWPLA
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHCHHHCCHHHHHHCCHHHHHHHHHHHHHHHH
AGRAFDRAVSIEPLHLQAQIEQCGAVVIVSTPAQLSRWPALPGFADLTPAPRAFFSSGGP
CCCHHHHEECCCCEEHHEEHHHCCEEEEEECCHHHHHCCCCCCCCCCCCCCHHHHCCCCC
LAPEAAQEYAAAYGAAPLEIYGSTETGGIAWRRQDQSDAWQPVAGIDVRRDEDGALNVRS
CCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCEEEECC
PHLDHTGWHRTDDRIAFDADGRFRLQGRLDRVLKLDGKRVSLPELEARLALHPYVAQAAI
CCCCCCCCCCCCCCEEECCCCCEEEHHHHHHHHHCCCCCCCCCHHHHHHHHCHHHHCEEE
VPLTGASRERVGAVVALTEAGGAALRDEGRVALAKMLRRHLAEYFDVVVLPRHWRFRVTL
EECCCCCHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCEEEEEEE
PFDARGKLPVAAVAAAFEPRSEGVEVLAEARSGDTLHYELRVPPTLVHFAGHFPGLPILP
CCCCCCCCCHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCHHHHHHCCCCCCCCCCC
GVVQIDWAMRLAADHLPAVRALASIDRLKFMAPVPPGAVLKLTLAHDAARRRVQFAYRVN
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEHHHHHHHHEEEEEEEC
GRDCASGVIVYGAPA
CCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9630474 [H]