Click here to switch to the map view.

The map label for this gene is paaF [C]

Identifier: 91784800

GI number: 91784800

Start: 3789421

End: 3790230

Strand: Reverse

Name: paaF [C]

Synonym: Bxe_A0993

Alternate gene names: 91784800

Gene position: 3790230-3789421 (Counterclockwise)

Preceding gene: 91784801

Following gene: 91784798

Centisome position: 77.42

GC content: 66.3

Gene sequence:

>810_bases
ATGAACGCCGAGGCGCGCAACGCTTCACTGGTCGAGATCGAACACGGCGCGTACGGTGTGCCGGGCGTCGTGCGTTTGAC
GATGAACCGGCCGGATGCCTTTAACGCGCTCTCCGAAGCCCTGCTCGACGACCTGCAAACGGCATTGAGCGAGATTGGCC
GGTCGGACGCGCGCGTGGTTGTGATCGCCGGCGCGGGCCGGGCGTTCTGCGCGGGACACGATCTGAAGGAAATGCGCGCG
GCGCCGTCGCTCGCGTACTACCAGGCGCTGTTCGCCCGCTGCACGAAGCTCATGATGACGATCCAGCGCCTGCCGCAACC
GGTGATCGCGCGCGTGCAAGGCATTGCCACCGCGGCCGGCTGCCAGCTCGTCGCAATGTGCGATCTGGCGGTCGCGGCCG
ACACGGCGCGTTTTGCCGTTTCGGGTGTGAATCTCGGGCTGTTCTGCGCGACGCCGTCGGTGCCGCTGTCGCGCAACCTG
TCGCGTAAGGCGGCGCTCGAAATGCTGCTCACCGGCGATTTTATCGACGCCGTGGGCGCGAAGCGGGAAGGTCTCGTGAA
CCGCGTGGTCCCGGCGGATGCGCTCGACGCGGAACTCGCGCGGCTCGCCGCCAGCATCTGCGCGAAACCCGTCGAAGCCG
TGAGCGCCGGAAAAGGTCTCTTCTACCGGCAACTGGAGATGGGTATCGAAGCGGCTTACCAGCTCGCCGGCCAGACGATG
GCCTGCAACATGATGGATGAGTCGGCGCTCGAAGGTGTGCAGGCGTTCATTGACAAACGGCCGCCCGACTGGAAACGGGA
CGCAGGCTAG

Upstream 100 bases:

>100_bases
CCGCGCTCGGGCGCAGCGTGGTTGCCCACATGCGCTCGCTGGCCGGGATCTGAGCCGCCGCACCGCGCGCGCTCGCCATC
CACCCAATGAGGAGTGAGTC

Downstream 100 bases:

>100_bases
CGGCGTCGTTCAAGGCGCCGTTCAGGTTCTAACCACCAGCCGTTTTTCGATCCACTCGATCAACGCCCCGATCACCCGAT
CGCGGTCCATGTCGTTCATC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNAEARNASLVEIEHGAYGVPGVVRLTMNRPDAFNALSEALLDDLQTALSEIGRSDARVVVIAGAGRAFCAGHDLKEMRA
APSLAYYQALFARCTKLMMTIQRLPQPVIARVQGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNL
SRKAALEMLLTGDFIDAVGAKREGLVNRVVPADALDAELARLAASICAKPVEAVSAGKGLFYRQLEMGIEAAYQLAGQTM
ACNMMDESALEGVQAFIDKRPPDWKRDAG

Sequences:

>Translated_269_residues
MNAEARNASLVEIEHGAYGVPGVVRLTMNRPDAFNALSEALLDDLQTALSEIGRSDARVVVIAGAGRAFCAGHDLKEMRA
APSLAYYQALFARCTKLMMTIQRLPQPVIARVQGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNL
SRKAALEMLLTGDFIDAVGAKREGLVNRVVPADALDAELARLAASICAKPVEAVSAGKGLFYRQLEMGIEAAYQLAGQTM
ACNMMDESALEGVQAFIDKRPPDWKRDAG
>Mature_269_residues
MNAEARNASLVEIEHGAYGVPGVVRLTMNRPDAFNALSEALLDDLQTALSEIGRSDARVVVIAGAGRAFCAGHDLKEMRA
APSLAYYQALFARCTKLMMTIQRLPQPVIARVQGIATAAGCQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNL
SRKAALEMLLTGDFIDAVGAKREGLVNRVVPADALDAELARLAASICAKPVEAVSAGKGLFYRQLEMGIEAAYQLAGQTM
ACNMMDESALEGVQAFIDKRPPDWKRDAG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=247, Percent_Identity=44.1295546558704, Blast_Score=208, Evalue=3e-54,
Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=31.496062992126, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI70995211, Length=253, Percent_Identity=29.2490118577075, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI260274832, Length=247, Percent_Identity=22.6720647773279, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI45643119, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI260275230, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=36.144578313253, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=28.3464566929134, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788597, Length=255, Percent_Identity=30.1960784313725, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=33.5907335907336, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787660, Length=267, Percent_Identity=30.7116104868914, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=38.8235294117647, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=30.4878048780488, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=32.4, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=27.1255060728745, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=184, Percent_Identity=30.9782608695652, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=210, Percent_Identity=30.4761904761905, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=186, Percent_Identity=29.0322580645161, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=26.4248704663212, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=37.8048780487805, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=31.9672131147541, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=31.9672131147541, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=28.6852589641434, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24650670, Length=271, Percent_Identity=26.9372693726937, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=222, Percent_Identity=26.5765765765766, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28500; Mature: 28500

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAEARNASLVEIEHGAYGVPGVVRLTMNRPDAFNALSEALLDDLQTALSEIGRSDARVV
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VIAGAGRAFCAGHDLKEMRAAPSLAYYQALFARCTKLMMTIQRLPQPVIARVQGIATAAG
EEECCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLSRKAALEMLLTGDFIDAVGA
HHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
KREGLVNRVVPADALDAELARLAASICAKPVEAVSAGKGLFYRQLEMGIEAAYQLAGQTM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHH
ACNMMDESALEGVQAFIDKRPPDWKRDAG
EEHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNAEARNASLVEIEHGAYGVPGVVRLTMNRPDAFNALSEALLDDLQTALSEIGRSDARVV
CCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
VIAGAGRAFCAGHDLKEMRAAPSLAYYQALFARCTKLMMTIQRLPQPVIARVQGIATAAG
EEECCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CQLVAMCDLAVAADTARFAVSGVNLGLFCATPSVPLSRNLSRKAALEMLLTGDFIDAVGA
HHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
KREGLVNRVVPADALDAELARLAASICAKPVEAVSAGKGLFYRQLEMGIEAAYQLAGQTM
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHH
ACNMMDESALEGVQAFIDKRPPDWKRDAG
EEHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA