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The map label for this gene is trmD [H]

Identifier: 91784785

GI number: 91784785

Start: 3774601

End: 3775368

Strand: Reverse

Name: trmD [H]

Synonym: Bxe_A1008

Alternate gene names: 91784785

Gene position: 3775368-3774601 (Counterclockwise)

Preceding gene: 91784786

Following gene: 91784784

Centisome position: 77.11

GC content: 61.72

Gene sequence:

>768_bases
ATGCAGTTCGATGTCGTCACGCTCTTTCCTGACATGTTTCGTGCGCTGACCGACTGGGGTATCACCAGCCGTGCGGCGAA
GCAGGAGCGCTACAGTTTGCGTACGTGGAATCCGCGCGATTTCACGACCGACAACTACCGCACAATCGACGATCGCCCGT
ACGGCGGCGGCCCCGGCATGGTGATGCTGGCGAGGCCGCTGGAAGACGCGATCGGCGCGGCGAAAGCGGCGCAGGCGGAG
CAGGGCATTGGCGCCCCGCGCGTCGTGATGATGTCGCCGCAAGGTGTCACGCTGAATCACGACCGTGTCATGCGGTTTGC
TGCCGAGCCTGGCCTGATCCTGTTGTGCGGGCGCTATGAAGCGATCGACCAGCGTTTGCTCGACCGTGTCGTCGACGAAG
AAGTCAGCCTTGGCGACTTCGTGCTGTCAGGCGGCGAATTGCCGGCTATGGCGTTGATGGATGCCGTGGTGCGTCAGTTG
CCCGGTGTGCTCAACGACTCGCAGTCTGCGGTGCAGGACAGTTTTGTCGACGTGCTGCTGGATTGTCCGCATTACACGCG
CCCCGAGGAATACAACGGCGTGCGTGTTCCAGATGTGCTGCTCGGCGGCCATCATGCGGAGATCGAAACGTGGCGTCGGC
GTGAAGCCTTGCGCAACACGTTGAACAAACGGCCCGATCTGATCGTGAAGGCCAGAAAGAACAAGATGTTGAGCCGTGCC
GACGAGGCATGGCTCGCGAGTCTCGCGAAGGAAGAGTCGAAGGCGTAA

Upstream 100 bases:

>100_bases
GTTCGTCGGCGTCTTTGTCAAAACAGTGGACCAGGCGGCGAAGCAGATCACCGTCGACTGGGAAGCTGATTACTAAATAA
TTCGCTGAATGGAGAGAGCG

Downstream 100 bases:

>100_bases
AGCCTCAGGTCTTTCGAGCGGACATAGGCGGCGCCGCGCATGCGTGCACGGCGCCAGATGTGAACCCATCCTCTATCGGG
GCCGTAGCCGAGCATTTTCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MQFDVVTLFPDMFRALTDWGITSRAAKQERYSLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAIGAAKAAQAE
QGIGAPRVVMMSPQGVTLNHDRVMRFAAEPGLILLCGRYEAIDQRLLDRVVDEEVSLGDFVLSGGELPAMALMDAVVRQL
PGVLNDSQSAVQDSFVDVLLDCPHYTRPEEYNGVRVPDVLLGGHHAEIETWRRREALRNTLNKRPDLIVKARKNKMLSRA
DEAWLASLAKEESKA

Sequences:

>Translated_255_residues
MQFDVVTLFPDMFRALTDWGITSRAAKQERYSLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAIGAAKAAQAE
QGIGAPRVVMMSPQGVTLNHDRVMRFAAEPGLILLCGRYEAIDQRLLDRVVDEEVSLGDFVLSGGELPAMALMDAVVRQL
PGVLNDSQSAVQDSFVDVLLDCPHYTRPEEYNGVRVPDVLLGGHHAEIETWRRREALRNTLNKRPDLIVKARKNKMLSRA
DEAWLASLAKEESKA
>Mature_255_residues
MQFDVVTLFPDMFRALTDWGITSRAAKQERYSLRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLARPLEDAIGAAKAAQAE
QGIGAPRVVMMSPQGVTLNHDRVMRFAAEPGLILLCGRYEAIDQRLLDRVVDEEVSLGDFVLSGGELPAMALMDAVVRQL
PGVLNDSQSAVQDSFVDVLLDCPHYTRPEEYNGVRVPDVLLGGHHAEIETWRRREALRNTLNKRPDLIVKARKNKMLSRA
DEAWLASLAKEESKA

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=254, Percent_Identity=51.1811023622047, Blast_Score=249, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28509; Mature: 28509

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVVTLFPDMFRALTDWGITSRAAKQERYSLRTWNPRDFTTDNYRTIDDRPYGGGPGM
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCE
VMLARPLEDAIGAAKAAQAEQGIGAPRVVMMSPQGVTLNHDRVMRFAAEPGLILLCGRYE
EEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECHHHHHHHHCCCCEEEEECCHH
AIDQRLLDRVVDEEVSLGDFVLSGGELPAMALMDAVVRQLPGVLNDSQSAVQDSFVDVLL
HHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
DCPHYTRPEEYNGVRVPDVLLGGHHAEIETWRRREALRNTLNKRPDLIVKARKNKMLSRA
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
DEAWLASLAKEESKA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFDVVTLFPDMFRALTDWGITSRAAKQERYSLRTWNPRDFTTDNYRTIDDRPYGGGPGM
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCE
VMLARPLEDAIGAAKAAQAEQGIGAPRVVMMSPQGVTLNHDRVMRFAAEPGLILLCGRYE
EEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECHHHHHHHHCCCCEEEEECCHH
AIDQRLLDRVVDEEVSLGDFVLSGGELPAMALMDAVVRQLPGVLNDSQSAVQDSFVDVLL
HHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
DCPHYTRPEEYNGVRVPDVLLGGHHAEIETWRRREALRNTLNKRPDLIVKARKNKMLSRA
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHH
DEAWLASLAKEESKA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA