Click here to switch to the map view.

The map label for this gene is pmbA [H]

Identifier: 91784774

GI number: 91784774

Start: 3764125

End: 3765495

Strand: Reverse

Name: pmbA [H]

Synonym: Bxe_A1019

Alternate gene names: 91784774

Gene position: 3765495-3764125 (Counterclockwise)

Preceding gene: 91784777

Following gene: 91784764

Centisome position: 76.91

GC content: 65.21

Gene sequence:

>1371_bases
ATGGCAGCAGACATGGACGTCAAGCAGCGCTTTTTTCCGCATACCCAGGATGAGCTGAAGGAAATCGCCTCGGACATCCT
TCGTCACGCGAAGTCGCTCGGCGGCACCGACGCGGCGACCGAAATCTCCGAAGGCGACGGCCTGTCCGTCTCCGTGCGGC
GCGGCGAAGTCGAGACGATCGAACACAACCGCGACAAGATGGTCGGCGTGACGGTGTTCATCGGCAACAAGCGCGGCAAT
GCGAGCACTTCGGACTTTTCGTCGCAGGCTTTGAAGGACACGGTCGCGGCGGCCTACAACATCGCGCGCTTCACGGCCGA
AGACGACTGCGCGGGTCTGGCCGAAGCCGAATTGCTGGAAACGGCGCCGCGCGATCTCGATCTCTATCACCCGTGGAATC
TGTCCGCGGACGAAGCGGTGGAAATTGCCCGCCGCGCGGAAGACGCCGCGTTCGCGACCGATCCGCAGATCAAAAACTCG
GAGGGCGCGAGCGTCTCGGCGCAGCACTCGCAGTTCGTGCTGGCCACCTCGCGCGGCTTCCTGGCGGGTTATCCGTACTC
GCGCCACTACATTGCGTGCGCGCCGATTGCGGGCAGCGGCCGCAACATGCAGCGCGACGACTGGTACACGTCCACCCGCA
GCGCCGAAGAACTGGCCAATCCGGAAGCGGTGGGCCGTTACGCGGCGCAGCGCGCGCTGGCGCGCATCGGCGCGCGCGGT
CTGGACACGCGCAAGGTGCCGGTACTGTTCGAAGCGCCGCTCGCCGCGGGCCTGCTCGGCGCATTCGTGCAAGCCACGAG
CGGCGGCGCGCTGTATCGCAAGACCACGTTCCTCGTCGACAGCCTGGGCAAGCCGGTGTTCGCGCCGCACGTGCAGGTGG
TCGAAGACCCGCATATTCCCCGCGCAATGGGCAGCGCGCCGTTCGACGAAGAAGGCGTGCGCACCAGACAGCGTTCGGTG
GTGAAAGACGGCGTGGTGGAAGGCTATTTCCTGTCCACCTACTCGGCACGCAAGCTCGGCATGCAGACTACCGGCAATGC
GGGCGGCTCGCACAACCTGTCGCTGCTGAGCTCGAACACGCGCCCCGAGGACGACTTCGAAGAGATGCTGCGCAAGCTCG
GCACGGGCCTGTTGCTGACCGAACTGATGGGGCAGGGCGTGAACTACGTGACGGGCGACTACTCGCGCGGTGCGTCGGGC
TTTTGGGTCGAGAACGGCAAGATCCAGTATCCGGTCGAGGAAATCACCGTGGCGAGCACGTTGCAGGAGATGTTCCACCA
CATCGTCGCGATCGGCGCGGATACGATCACGCGCGGCACCAAGAAAACCGGTTCGGTGCTGATCGAGCGCATGACGATCG
CGGGGCAGTGA

Upstream 100 bases:

>100_bases
ATGCGCGAGGCATGTGCTCAACCGGTGCGAATTCGGCGAACACAGCAAACACAGCGAACACACACAGGGCCGCTCGGCCC
CGAACAGGACGGCAACGACA

Downstream 100 bases:

>100_bases
AGCGGTTATGCCGCGTGCCGGTCGGCCTCCGGTTCGACCGTTGAAACGAAAACGCCACGCGAGTCATTGCGTGGCGTTTT
TTCGTATCAGGCGGGCGGCG

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAADMDVKQRFFPHTQDELKEIASDILRHAKSLGGTDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGN
ASTSDFSSQALKDTVAAAYNIARFTAEDDCAGLAEAELLETAPRDLDLYHPWNLSADEAVEIARRAEDAAFATDPQIKNS
EGASVSAQHSQFVLATSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSTRSAEELANPEAVGRYAAQRALARIGARG
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVVEDPHIPRAMGSAPFDEEGVRTRQRSV
VKDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLSLLSSNTRPEDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASG
FWVENGKIQYPVEEITVASTLQEMFHHIVAIGADTITRGTKKTGSVLIERMTIAGQ

Sequences:

>Translated_456_residues
MAADMDVKQRFFPHTQDELKEIASDILRHAKSLGGTDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGN
ASTSDFSSQALKDTVAAAYNIARFTAEDDCAGLAEAELLETAPRDLDLYHPWNLSADEAVEIARRAEDAAFATDPQIKNS
EGASVSAQHSQFVLATSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSTRSAEELANPEAVGRYAAQRALARIGARG
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVVEDPHIPRAMGSAPFDEEGVRTRQRSV
VKDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLSLLSSNTRPEDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASG
FWVENGKIQYPVEEITVASTLQEMFHHIVAIGADTITRGTKKTGSVLIERMTIAGQ
>Mature_455_residues
AADMDVKQRFFPHTQDELKEIASDILRHAKSLGGTDAATEISEGDGLSVSVRRGEVETIEHNRDKMVGVTVFIGNKRGNA
STSDFSSQALKDTVAAAYNIARFTAEDDCAGLAEAELLETAPRDLDLYHPWNLSADEAVEIARRAEDAAFATDPQIKNSE
GASVSAQHSQFVLATSRGFLAGYPYSRHYIACAPIAGSGRNMQRDDWYTSTRSAEELANPEAVGRYAAQRALARIGARGL
DTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVVEDPHIPRAMGSAPFDEEGVRTRQRSVV
KDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLSLLSSNTRPEDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASGF
WVENGKIQYPVEEITVASTLQEMFHHIVAIGADTITRGTKKTGSVLIERMTIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=439, Percent_Identity=47.8359908883827, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1789640, Length=400, Percent_Identity=23.5, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49257; Mature: 49125

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADMDVKQRFFPHTQDELKEIASDILRHAKSLGGTDAATEISEGDGLSVSVRRGEVETI
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGNKRGNASTSDFSSQALKDTVAAAYNIARFTAEDDCAGLAEAELLE
HCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
TAPRDLDLYHPWNLSADEAVEIARRAEDAAFATDPQIKNSEGASVSAQHSQFVLATSRGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCC
LAGYPYSRHYIACAPIAGSGRNMQRDDWYTSTRSAEELANPEAVGRYAAQRALARIGARG
EECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCC
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVVEDPHIP
CCCCCCCEEEECHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCEEECCCCCC
RAMGSAPFDEEGVRTRQRSVVKDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLSLLSSNT
HHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCCCCCEEEEECCC
RPEDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASGFWVENGKIQYPVEEITVAST
CCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFHHIVAIGADTITRGTKKTGSVLIERMTIAGQ
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AADMDVKQRFFPHTQDELKEIASDILRHAKSLGGTDAATEISEGDGLSVSVRRGEVETI
CCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHH
EHNRDKMVGVTVFIGNKRGNASTSDFSSQALKDTVAAAYNIARFTAEDDCAGLAEAELLE
HCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
TAPRDLDLYHPWNLSADEAVEIARRAEDAAFATDPQIKNSEGASVSAQHSQFVLATSRGF
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCC
LAGYPYSRHYIACAPIAGSGRNMQRDDWYTSTRSAEELANPEAVGRYAAQRALARIGARG
EECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCC
LDTRKVPVLFEAPLAAGLLGAFVQATSGGALYRKTTFLVDSLGKPVFAPHVQVVEDPHIP
CCCCCCCEEEECHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCEEECCCCCC
RAMGSAPFDEEGVRTRQRSVVKDGVVEGYFLSTYSARKLGMQTTGNAGGSHNLSLLSSNT
HHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCCCCCCCEEEEECCC
RPEDDFEEMLRKLGTGLLLTELMGQGVNYVTGDYSRGASGFWVENGKIQYPVEEITVAST
CCCHHHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCCEEEECCEEECCHHHHHHHHH
LQEMFHHIVAIGADTITRGTKKTGSVLIERMTIAGQ
HHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]