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The map label for this gene is amnG

Identifier: 91784642

GI number: 91784642

Start: 3632776

End: 3633813

Strand: Reverse

Name: amnG

Synonym: Bxe_A1152

Alternate gene names: 91784642

Gene position: 3633813-3632776 (Counterclockwise)

Preceding gene: 91784643

Following gene: 91784641

Centisome position: 74.22

GC content: 59.83

Gene sequence:

>1038_bases
ATGAGCATCAACGGGAACGCCGCAGCGTCCAAGCTTTATATTTCCGACGTCACTTTGCGCGATGGAAGCCACGCTATCCG
CCATCAATACTCTGTGCCTCAGGTACGTCAGATCGCCCTGGCGCTGGACGAGGCCCGCGTTGACTCCATAGAGGTCGCGC
ACGGGGATGGCCTTCAGGGTTCGAGCTTCAACTACGGCTTTGGCGCTCACACCGACGTGGAATGGATCGAAGCTGTAGCC
GATGTTGTGAAGCACGCCAAGATCGCGACACTCCTGCTGCCAGGCATAGGCACCGTGCATGATCTGCAGGCCGCGTACAA
CGCCGGCGCGCGCATCGTGCGTGTCGCCACCCACTGCACTGAAGCCGATGTGTCGCGCCAGCACATCGAACTCGCTCGCA
AGCTGGACATGGACACGGTGGGCTTCCTAATGATGAGTCACATGTCCTCGCCGCAAGCTCTGGCTGAGCAGGCCAAGCTC
ATGGAAAGCTACGGCGCGACCTGCTGCTATGTCGTCGATTCCGGCGGCGCGATGAACATGAACGATGTTCGAGATCGTGT
TCTCGCGCTGCGTGAAGTCTTGCAGCCGGAAACTGCCATTGGGATTCATGCGCATCACAATCTCAGCCTCGGCGTGGCCA
ATTCCATCGTGGCCGTAGAAGCAGGCGCTATCCGTGTCGACGCAAGCCTGGCTGGCATGGGCGCGGGTGCCGGGAATGCG
CCGTTGGAGGTATTCATCGCCGCCGCGGAACGCATGGGCTGGCAGCATGGTACGGACCTGTACAGATTGATGGATGCCGC
CGATGACATCGTTCGCCCTCTGCAAGATCGTCCAGTGCGTGTTGATCGAGAGACGCTCGCGCTCGGCTATGCCGGTGTCT
ACAGCTCATTCCTGCGGCACGCCGAGGCCGCCGGTCACAAGTATGGTCTGAAGACGGTCGATATTCTGGTGGAGCTTGGC
AAGCGTCGTATGGTTGGTGGGCAAGAAGACATGATCGTCGATGTGGCGCTCGATTTGGCACAACAGACGACAACGTAG

Upstream 100 bases:

>100_bases
AAATCTCGACATCATGACCAGCGCCGGCCTTCGCACAGCCGAACAAATGGCAGCACGGATGACGGCCGGGACGGTCGCCG
CCTGAACGAGGAGCACAACG

Downstream 100 bases:

>100_bases
CTAGCGGTTAGACGAATGGCATCGGGGAGCAGCTACTCGCTCCGCTCTAGGCGTTTGGGAAAACGCATTACCCATGCCGA
ACCTATTGAGCCGATCCACA

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 2; 4-hydroxy-2-keto-pentanoic acid aldolase 2; 4-hydroxy-2-oxopentanoate aldolase 2

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSINGNAAASKLYISDVTLRDGSHAIRHQYSVPQVRQIALALDEARVDSIEVAHGDGLQGSSFNYGFGAHTDVEWIEAVA
DVVKHAKIATLLLPGIGTVHDLQAAYNAGARIVRVATHCTEADVSRQHIELARKLDMDTVGFLMMSHMSSPQALAEQAKL
MESYGATCCYVVDSGGAMNMNDVRDRVLALREVLQPETAIGIHAHHNLSLGVANSIVAVEAGAIRVDASLAGMGAGAGNA
PLEVFIAAAERMGWQHGTDLYRLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEAAGHKYGLKTVDILVELG
KRRMVGGQEDMIVDVALDLAQQTTT

Sequences:

>Translated_345_residues
MSINGNAAASKLYISDVTLRDGSHAIRHQYSVPQVRQIALALDEARVDSIEVAHGDGLQGSSFNYGFGAHTDVEWIEAVA
DVVKHAKIATLLLPGIGTVHDLQAAYNAGARIVRVATHCTEADVSRQHIELARKLDMDTVGFLMMSHMSSPQALAEQAKL
MESYGATCCYVVDSGGAMNMNDVRDRVLALREVLQPETAIGIHAHHNLSLGVANSIVAVEAGAIRVDASLAGMGAGAGNA
PLEVFIAAAERMGWQHGTDLYRLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEAAGHKYGLKTVDILVELG
KRRMVGGQEDMIVDVALDLAQQTTT
>Mature_344_residues
SINGNAAASKLYISDVTLRDGSHAIRHQYSVPQVRQIALALDEARVDSIEVAHGDGLQGSSFNYGFGAHTDVEWIEAVAD
VVKHAKIATLLLPGIGTVHDLQAAYNAGARIVRVATHCTEADVSRQHIELARKLDMDTVGFLMMSHMSSPQALAEQAKLM
ESYGATCCYVVDSGGAMNMNDVRDRVLALREVLQPETAIGIHAHHNLSLGVANSIVAVEAGAIRVDASLAGMGAGAGNAP
LEVFIAAAERMGWQHGTDLYRLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAEAAGHKYGLKTVDILVELGK
RRMVGGQEDMIVDVALDLAQQTTT

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=330, Percent_Identity=83.030303030303, Blast_Score=545, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA2_BURXL (Q13VU3)

Other databases:

- EMBL:   CP000270
- RefSeq:   YP_559848.1
- ProteinModelPortal:   Q13VU3
- SMR:   Q13VU3
- GeneID:   4002973
- GenomeReviews:   CP000270_GR
- KEGG:   bxe:Bxe_A1152
- HOGENOM:   HBG300122
- OMA:   HERIRSE
- ProtClustDB:   PRK08195
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 37004; Mature: 36872

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 174-174 BINDING 203-203 BINDING 294-294

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINGNAAASKLYISDVTLRDGSHAIRHQYSVPQVRQIALALDEARVDSIEVAHGDGLQG
CCCCCCCCCCEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
SSFNYGFGAHTDVEWIEAVADVVKHAKIATLLLPGIGTVHDLQAAYNAGARIVRVATHCT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEHHHHCC
EADVSRQHIELARKLDMDTVGFLMMSHMSSPQALAEQAKLMESYGATCCYVVDSGGAMNM
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCH
NDVRDRVLALREVLQPETAIGIHAHHNLSLGVANSIVAVEAGAIRVDASLAGMGAGAGNA
HHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCEEEEECCCEEECCHHHCCCCCCCCC
PLEVFIAAAERMGWQHGTDLYRLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRH
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHH
AEAAGHKYGLKTVDILVELGKRRMVGGQEDMIVDVALDLAQQTTT
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCC
>Mature Secondary Structure 
SINGNAAASKLYISDVTLRDGSHAIRHQYSVPQVRQIALALDEARVDSIEVAHGDGLQG
CCCCCCCCCEEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
SSFNYGFGAHTDVEWIEAVADVVKHAKIATLLLPGIGTVHDLQAAYNAGARIVRVATHCT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEHHHHCC
EADVSRQHIELARKLDMDTVGFLMMSHMSSPQALAEQAKLMESYGATCCYVVDSGGAMNM
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCH
NDVRDRVLALREVLQPETAIGIHAHHNLSLGVANSIVAVEAGAIRVDASLAGMGAGAGNA
HHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCEEEEECCCEEECCHHHCCCCCCCCC
PLEVFIAAAERMGWQHGTDLYRLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRH
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHH
AEAAGHKYGLKTVDILVELGKRRMVGGQEDMIVDVALDLAQQTTT
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA