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The map label for this gene is greA [H]

Identifier: 91784541

GI number: 91784541

Start: 3528784

End: 3529314

Strand: Reverse

Name: greA [H]

Synonym: Bxe_A1258

Alternate gene names: 91784541

Gene position: 3529314-3528784 (Counterclockwise)

Preceding gene: 91784542

Following gene: 91784540

Centisome position: 72.09

GC content: 58.95

Gene sequence:

>531_bases
ATGCACTTAACCGCGGTGATTTTTTTTACGGCTGTTTTTTGCATAGAGTTGTTTATGAGCACTGTTCCATTGACTAAGCG
CGGCGCGGAAATGTTGCGCGATGAATTGCAGCGCCTGAAATCGGTTGAGCGTCCCTCGGTGATCAATTCGATCGCGGAAG
CGCGTGCCCAGGGCGATCTGTCGGAAAACGCGGAATACGACGCGGCGAAGGAAAAGCAGGGCTTTATCGAAGGCCGGATC
GCCGAGATCGAATCCAAGCTGGCCGGCGCGCAGGTGATCGATCCGGCGGCGGTGGAGGCGGATGGTCGCGTGGTGTTCGG
TGCAACGGTGGAGCTGGAAGACCTGAATTCGGGTGCGTCCGTCAAATATCAGATCGTCGGCGACGACGAAGCGGATATCG
ACCACGGCCTGATCTCGATCAGCTCGCCGATTGCGCGCGCCCTGATCGGCAAGTCGGAGGGCGACGTTGCGTCGGTGCAG
GCACCGGGCGGCGTGCGCGAATACGAAATCATCGCGGTCAGCTACGTTTGA

Upstream 100 bases:

>100_bases
TAAGGCTTCGAATACCTGTCCAAGATGTAAGTGCCGCGGTTAAGCGGCGTTCCGCAGGTGCTTGGCGCCGGGTTTTGCCC
GGTGCTCTGGCCCCCGCGGA

Downstream 100 bases:

>100_bases
ACGCGCCGCTGGTGCCCCAGATGCCACATCGACTGTTCCGGTTATTGACGGTGGTATGGGTCGGCAGTCTGCTGACCATC
GGCTATGCCGTCGCGCCCGT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MHLTAVIFFTAVFCIELFMSTVPLTKRGAEMLRDELQRLKSVERPSVINSIAEARAQGDLSENAEYDAAKEKQGFIEGRI
AEIESKLAGAQVIDPAAVEADGRVVFGATVELEDLNSGASVKYQIVGDDEADIDHGLISISSPIARALIGKSEGDVASVQ
APGGVREYEIIAVSYV

Sequences:

>Translated_176_residues
MHLTAVIFFTAVFCIELFMSTVPLTKRGAEMLRDELQRLKSVERPSVINSIAEARAQGDLSENAEYDAAKEKQGFIEGRI
AEIESKLAGAQVIDPAAVEADGRVVFGATVELEDLNSGASVKYQIVGDDEADIDHGLISISSPIARALIGKSEGDVASVQ
APGGVREYEIIAVSYV
>Mature_176_residues
MHLTAVIFFTAVFCIELFMSTVPLTKRGAEMLRDELQRLKSVERPSVINSIAEARAQGDLSENAEYDAAKEKQGFIEGRI
AEIESKLAGAQVIDPAAVEADGRVVFGATVELEDLNSGASVKYQIVGDDEADIDHGLISISSPIARALIGKSEGDVASVQ
APGGVREYEIIAVSYV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=58.2278481012658, Blast_Score=186, Evalue=9e-49,
Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=37.3417721518987, Blast_Score=100, Evalue=7e-23,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18826; Mature: 18826

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLTAVIFFTAVFCIELFMSTVPLTKRGAEMLRDELQRLKSVERPSVINSIAEARAQGDL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
SENAEYDAAKEKQGFIEGRIAEIESKLAGAQVIDPAAVEADGRVVFGATVELEDLNSGAS
CCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCC
VKYQIVGDDEADIDHGLISISSPIARALIGKSEGDVASVQAPGGVREYEIIAVSYV
EEEEEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEC
>Mature Secondary Structure
MHLTAVIFFTAVFCIELFMSTVPLTKRGAEMLRDELQRLKSVERPSVINSIAEARAQGDL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
SENAEYDAAKEKQGFIEGRIAEIESKLAGAQVIDPAAVEADGRVVFGATVELEDLNSGAS
CCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCC
VKYQIVGDDEADIDHGLISISSPIARALIGKSEGDVASVQAPGGVREYEIIAVSYV
EEEEEECCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]