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The map label for this gene is trmJ [H]

Identifier: 91784181

GI number: 91784181

Start: 3119939

End: 3120775

Strand: Reverse

Name: trmJ [H]

Synonym: Bxe_A1620

Alternate gene names: 91784181

Gene position: 3120775-3119939 (Counterclockwise)

Preceding gene: 91784186

Following gene: 91784180

Centisome position: 63.74

GC content: 68.94

Gene sequence:

>837_bases
GTGGACCACAGCCACCATTCTTCCGATCCTGCCGTAGCCGAATTGCGCGGCGGCTTCACCTCGACCCGCTTCGTGCTCGT
CGAGCCGAGCCATCCAGGCAATGTCGGCGCCGCGGCGCGCGCGCTGAAAACCATGGGTTTCTCGCGGCTCGTGCTGGTGT
CGCCACGCGTGCCGCACGTACAGACCGATCCGGAGGCCATCGCTATGGCGAGCGGCGCCGACGACGTGCTGGCGTGCGCG
CACGTCGTGCCGACGCTCGCCGACGCCCTGAGCGGCGTGCATTGGTCGATCGCGCTGACCGCGCGGCTGCGCGAATACGG
GCCGCCGCAATGGACGCCGCGCGCCGCGGCCGGCATCGCGCGCGACCAGGCAGTGCACGGCGAGATCGCGCTGGTGTTCG
GCAACGAGCGCACGGGTTTGTCGAACGAGGACGTCGAGCGGTGCAGCGCGCTCGCGCATATTCCGGCCAATCCGGCCTAT
AGCTCGCTCAATCTCGCGCAGGCCGTGCAGGTGCTCGCGTACGAGTTGCGCACCACCTATCTCTCGGCCGATAGCGCAAC
GGCCACGGCGCACGCCGCGCCTCGGGCGGCGGCGGGACCGGGCGAACCGGCCGGCGCGCGCGCGGCGAGCGACGAAATCG
AAAGCATGTTCACGCATCTGGAAAGCGCGCTGGTCGCGCTCGAATTTCTCGATCCGGCCAATCCGAAGAAACTGATGTCG
CGTCTGCGGCGGCTGTTCGCGCGCTCCGGCCTGGAGCGCGAGGAAGTGAATATCGTGCGGGGCATCGCCAAACACATTCT
GTTGAAGACCGGACCCAGGGGCGGCGACGCGTCCTGA

Upstream 100 bases:

>100_bases
GACGTATTCGCGCGCGCGCTCATGCCCGCGCTTTCACGCAATCGGGACCGGCCGCGGCGTTGGCGCTACCATACGCGTCT
TTAGTTTGCCGAATTCCATC

Downstream 100 bases:

>100_bases
GAACGGCATGCGTTCTGATAGCCGACAGCCAGCTAGCCAGCTAGCCAGCTAGCCAGCTAGCCGGATAGCTGGCGGCAACC
CCGCGGGCAGCGTGGGCTTG

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MDHSHHSSDPAVAELRGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVPHVQTDPEAIAMASGADDVLACA
HVVPTLADALSGVHWSIALTARLREYGPPQWTPRAAAGIARDQAVHGEIALVFGNERTGLSNEDVERCSALAHIPANPAY
SSLNLAQAVQVLAYELRTTYLSADSATATAHAAPRAAAGPGEPAGARAASDEIESMFTHLESALVALEFLDPANPKKLMS
RLRRLFARSGLEREEVNIVRGIAKHILLKTGPRGGDAS

Sequences:

>Translated_278_residues
MDHSHHSSDPAVAELRGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVPHVQTDPEAIAMASGADDVLACA
HVVPTLADALSGVHWSIALTARLREYGPPQWTPRAAAGIARDQAVHGEIALVFGNERTGLSNEDVERCSALAHIPANPAY
SSLNLAQAVQVLAYELRTTYLSADSATATAHAAPRAAAGPGEPAGARAASDEIESMFTHLESALVALEFLDPANPKKLMS
RLRRLFARSGLEREEVNIVRGIAKHILLKTGPRGGDAS
>Mature_278_residues
MDHSHHSSDPAVAELRGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVPHVQTDPEAIAMASGADDVLACA
HVVPTLADALSGVHWSIALTARLREYGPPQWTPRAAAGIARDQAVHGEIALVFGNERTGLSNEDVERCSALAHIPANPAY
SSLNLAQAVQVLAYELRTTYLSADSATATAHAAPRAAAGPGEPAGARAASDEIESMFTHLESALVALEFLDPANPKKLMS
RLRRLFARSGLEREEVNIVRGIAKHILLKTGPRGGDAS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=249, Percent_Identity=41.3654618473896, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1790865, Length=168, Percent_Identity=37.5, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29319; Mature: 29319

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHSHHSSDPAVAELRGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVPHV
CCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
QTDPEAIAMASGADDVLACAHVVPTLADALSGVHWSIALTARLREYGPPQWTPRAAAGIA
CCCHHHHEECCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCHHHHHHH
RDQAVHGEIALVFGNERTGLSNEDVERCSALAHIPANPAYSSLNLAQAVQVLAYELRTTY
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LSADSATATAHAAPRAAAGPGEPAGARAASDEIESMFTHLESALVALEFLDPANPKKLMS
CCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RLRRLFARSGLEREEVNIVRGIAKHILLKTGPRGGDAS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDHSHHSSDPAVAELRGGFTSTRFVLVEPSHPGNVGAAARALKTMGFSRLVLVSPRVPHV
CCCCCCCCCCHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCC
QTDPEAIAMASGADDVLACAHVVPTLADALSGVHWSIALTARLREYGPPQWTPRAAAGIA
CCCHHHHEECCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCHHHHHHH
RDQAVHGEIALVFGNERTGLSNEDVERCSALAHIPANPAYSSLNLAQAVQVLAYELRTTY
HHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LSADSATATAHAAPRAAAGPGEPAGARAASDEIESMFTHLESALVALEFLDPANPKKLMS
CCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RLRRLFARSGLEREEVNIVRGIAKHILLKTGPRGGDAS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA