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The map label for this gene is ligA [H]

Identifier: 91784125

GI number: 91784125

Start: 3054296

End: 3056362

Strand: Reverse

Name: ligA [H]

Synonym: Bxe_A1676

Alternate gene names: 91784125

Gene position: 3056362-3054296 (Counterclockwise)

Preceding gene: 91784126

Following gene: 91784124

Centisome position: 62.43

GC content: 65.22

Gene sequence:

>2067_bases
ATGGCCCGAACCCCCGTCTCACCTCCCGCAACCAGCGCCCCGGCAGAGCGTGCCGCGTGGCTGCGCGCGGAACTCGAACG
CGCCAATTACGCGTACTACGTGCTCGACCAGCCGGACCTGCCCGACGCGGAATACGACCGGCTGTTCAAGGAACTCGAGA
GCATCGAGACGGAACATCCCGACCTGATCGTGCCGGATTCGCCCACCCAGCGCGTGGGCGGCGAGGCGGCGAGCGGTTTC
GAGCCGGTTGTGCACGAGCAGCCCATGCTGTCGCTGAACAACGGTTTCGCGGACGAGGACATCGTCGCGTTCGACAAGCG
CGTTGGCGACGCGCTCGGCAAGAATGCGAGCGAGCCGCCGGTGCCGGTCGAATACGCGGCCGAGCTGAAGTTCGACGGCC
TCGCCATTTCGCTGCGTTATGTCGACGGCGTATTCGTCCAGGCGTCCACGCGCGGCGACGGCACGACCGGCGAGAACGTC
ACCGAAAACGTCCGCACCATCCGCTCCATTCCGCTCAGGCTCAAGGGCAAACGCGTGCCGCACGTGCTCGACGTGCGTGG
CGAAGTGCTGATGTTCAAGCGCGATTTCGAACGTCTGAACGAACGCCAGCGGGCCGCGGAACAAAAGGAGTTCGCCAATC
CGCGCAATGCGGCGGCGGGCAGCTTGCGGCAGCTCGATTCGAAGATCACCGCGCAGCGGCCGCTGTCGTTTTTCTCTTAT
GGAATCGGCGTGCTCGAAGGCATGGACATGCCGGCCACGCACAGTGAACTGCTCGACTGGTACAAGGAGCTCGGCTTGCC
GGTGAACGGCGAACGGGCGGTGGTGCACGGCGCGGAGGGTTTGCTGGGCTTTTTCCACGCCGTTGGTGAAAAGCGCGAGA
AGCTGCCGTACGACATCGACGGGGTGGTCTACAAGGTCAACCGGCGCGATGAGCAGGAGGCGCTCGGCTTCGTGTCGCGC
GCGCCGCGTTTTGCGCTGGCGCACAAATTCCCCGCGCAGGAAGCGCTCACCCGGCTGGTCGCGATCGACGTGCAGGTCGG
CCGCACCGGTGCGGTCACGCCGGTGGCGCGGCTGGAACCGGTGTTCGTGGGCGGCGCGACGGTCACGAATGCCACGCTGC
ACAACGAAGACGAGGTGCGCCGCAAGGATATCCGCATCGGCGACACGGTGATCGTGCGGCGTGCCGGCGACGTGATTCCC
GAAGTGGTGAGCGCGCTGCTCGACCGGCGCCCCGACGACGCCCGCGAATTCGTGATGCCCACGCATTGCCCGGTATGCGG
CTCGAACATCGAGCGGCTGCCGGACGAGGCGATTGCGCGCTGTACCGGCGGCCTGTTCTGTCCCGCGCAGCGCAAGCAGG
CGTTGTGGCATTTCGCGCAGCGGCGCGCGCTGGATATCGACGGCCTTGGCGAAAAGATCATCGATCAACTGGTCGAGCAG
AATCTGGTGCGCACACCGGCCGATCTGTTCAACCTCGGCTTCGCGACGCTCGCCGAACTCGACCGCTTTGCCGAGAAGTC
CGCGCAGAACCTGCTCGATTCGCTCGAAAAGGCCAAGCACACCACGTTGGCGCGTTTTATCTACGCGCTCGGGATTCGCC
ATGTGGGCGAGTCGACGGCGAAAGACCTGGCCAAACATTTCGGCTCGCTGGATCCGATCATGGACGCGTCGGTCGAAGCC
TTGCTGGAAGTCAACGACGTCGGGCCGGTAGTGGCCGAGTCGATTCATCAGTTCTTCGCCGAAGAGCACAACCGCACGGT
GATCGAACAACTGCGCGCGCCGGGCCGGGTCACGTGGCCGGAGGGGCCGCCCGCGCCCAAAGCGCCGCAAGGCGTCTTGG
CCGGCAAGACGGTGGTGTTGACCGGCACCTTGCCGAGCCTCGCCCGCGAAGAAGCGAAGGAAATGCTGGAAGCAGCGGGC
GCGAAAGTGGCTGGATCCGTGTCGAAGAAAACGGATTATGTGGTGGCGGGGGCCGAAGCGGGCAGCAAGTTGGCCAAGGC
CGAGGAACTCGGCATTCCCGTACTCGACGAAGATGGTATGCGTAAGCTCCTGGAGGGGCAGTTATGA

Upstream 100 bases:

>100_bases
GACGCGCCGCGCGCCGAAACTGGCCATTCGGCCGATGTAAAGGGGCACGCTTCCTGCGATAATCCAATGTCTGAATTTCA
CTGAAGAACCGTCCGACAGC

Downstream 100 bases:

>100_bases
TCCGCGAAATTCTCAAGATGGGCGATCCGCGCCTGTTGCGCATTGCCGATCCGGTCGATCACTTCGACACGCCCGAACTG
CATGAACTCGTGAAGGACAT

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MARTPVSPPATSAPAERAAWLRAELERANYAYYVLDQPDLPDAEYDRLFKELESIETEHPDLIVPDSPTQRVGGEAASGF
EPVVHEQPMLSLNNGFADEDIVAFDKRVGDALGKNASEPPVPVEYAAELKFDGLAISLRYVDGVFVQASTRGDGTTGENV
TENVRTIRSIPLRLKGKRVPHVLDVRGEVLMFKRDFERLNERQRAAEQKEFANPRNAAAGSLRQLDSKITAQRPLSFFSY
GIGVLEGMDMPATHSELLDWYKELGLPVNGERAVVHGAEGLLGFFHAVGEKREKLPYDIDGVVYKVNRRDEQEALGFVSR
APRFALAHKFPAQEALTRLVAIDVQVGRTGAVTPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIP
EVVSALLDRRPDDAREFVMPTHCPVCGSNIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQ
NLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDASVEA
LLEVNDVGPVVAESIHQFFAEEHNRTVIEQLRAPGRVTWPEGPPAPKAPQGVLAGKTVVLTGTLPSLAREEAKEMLEAAG
AKVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRKLLEGQL

Sequences:

>Translated_688_residues
MARTPVSPPATSAPAERAAWLRAELERANYAYYVLDQPDLPDAEYDRLFKELESIETEHPDLIVPDSPTQRVGGEAASGF
EPVVHEQPMLSLNNGFADEDIVAFDKRVGDALGKNASEPPVPVEYAAELKFDGLAISLRYVDGVFVQASTRGDGTTGENV
TENVRTIRSIPLRLKGKRVPHVLDVRGEVLMFKRDFERLNERQRAAEQKEFANPRNAAAGSLRQLDSKITAQRPLSFFSY
GIGVLEGMDMPATHSELLDWYKELGLPVNGERAVVHGAEGLLGFFHAVGEKREKLPYDIDGVVYKVNRRDEQEALGFVSR
APRFALAHKFPAQEALTRLVAIDVQVGRTGAVTPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIP
EVVSALLDRRPDDAREFVMPTHCPVCGSNIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQ
NLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDASVEA
LLEVNDVGPVVAESIHQFFAEEHNRTVIEQLRAPGRVTWPEGPPAPKAPQGVLAGKTVVLTGTLPSLAREEAKEMLEAAG
AKVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRKLLEGQL
>Mature_687_residues
ARTPVSPPATSAPAERAAWLRAELERANYAYYVLDQPDLPDAEYDRLFKELESIETEHPDLIVPDSPTQRVGGEAASGFE
PVVHEQPMLSLNNGFADEDIVAFDKRVGDALGKNASEPPVPVEYAAELKFDGLAISLRYVDGVFVQASTRGDGTTGENVT
ENVRTIRSIPLRLKGKRVPHVLDVRGEVLMFKRDFERLNERQRAAEQKEFANPRNAAAGSLRQLDSKITAQRPLSFFSYG
IGVLEGMDMPATHSELLDWYKELGLPVNGERAVVHGAEGLLGFFHAVGEKREKLPYDIDGVVYKVNRRDEQEALGFVSRA
PRFALAHKFPAQEALTRLVAIDVQVGRTGAVTPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPE
VVSALLDRRPDDAREFVMPTHCPVCGSNIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQN
LVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDASVEAL
LEVNDVGPVVAESIHQFFAEEHNRTVIEQLRAPGRVTWPEGPPAPKAPQGVLAGKTVVLTGTLPSLAREEAKEMLEAAGA
KVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGMRKLLEGQL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=46.1538461538462, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=666, Percent_Identity=58.4084084084084, Blast_Score=764, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=425, Percent_Identity=26.5882352941176, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324791, Length=90, Percent_Identity=41.1111111111111, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI62472391, Length=92, Percent_Identity=46.7391304347826, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737765, Length=92, Percent_Identity=46.7391304347826, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75269; Mature: 75138

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTPVSPPATSAPAERAAWLRAELERANYAYYVLDQPDLPDAEYDRLFKELESIETEHP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
DLIVPDSPTQRVGGEAASGFEPVVHEQPMLSLNNGFADEDIVAFDKRVGDALGKNASEPP
CEECCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPVEYAAELKFDGLAISLRYVDGVFVQASTRGDGTTGENVTENVRTIRSIPLRLKGKRVP
CCHHHHHCEEECCEEEEEEEECEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEECCCCCC
HVLDVRGEVLMFKRDFERLNERQRAAEQKEFANPRNAAAGSLRQLDSKITAQRPLSFFSY
EEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
GIGVLEGMDMPATHSELLDWYKELGLPVNGERAVVHGAEGLLGFFHAVGEKREKLPYDID
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCC
GVVYKVNRRDEQEALGFVSRAPRFALAHKFPAQEALTRLVAIDVQVGRTGAVTPVARLEP
CEEEEECCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHCCE
VFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVSALLDRRPDDAREFVMP
EEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHCCCC
THCPVCGSNIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQ
CCCCCCCCCHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
NLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KDLAKHFGSLDPIMDASVEALLEVNDVGPVVAESIHQFFAEEHNRTVIEQLRAPGRVTWP
HHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEECC
EGPPAPKAPQGVLAGKTVVLTGTLPSLAREEAKEMLEAAGAKVAGSVSKKTDYVVAGAEA
CCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCC
GSKLAKAEELGIPVLDEDGMRKLLEGQL
CHHHHHHHHCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
ARTPVSPPATSAPAERAAWLRAELERANYAYYVLDQPDLPDAEYDRLFKELESIETEHP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
DLIVPDSPTQRVGGEAASGFEPVVHEQPMLSLNNGFADEDIVAFDKRVGDALGKNASEPP
CEECCCCCHHHCCCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VPVEYAAELKFDGLAISLRYVDGVFVQASTRGDGTTGENVTENVRTIRSIPLRLKGKRVP
CCHHHHHCEEECCEEEEEEEECEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEECCCCCC
HVLDVRGEVLMFKRDFERLNERQRAAEQKEFANPRNAAAGSLRQLDSKITAQRPLSFFSY
EEECCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHH
GIGVLEGMDMPATHSELLDWYKELGLPVNGERAVVHGAEGLLGFFHAVGEKREKLPYDID
HHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCC
GVVYKVNRRDEQEALGFVSRAPRFALAHKFPAQEALTRLVAIDVQVGRTGAVTPVARLEP
CEEEEECCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHCCE
VFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVSALLDRRPDDAREFVMP
EEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHCCCC
THCPVCGSNIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVEQ
CCCCCCCCCHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
NLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYALGIRHVGESTA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
KDLAKHFGSLDPIMDASVEALLEVNDVGPVVAESIHQFFAEEHNRTVIEQLRAPGRVTWP
HHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEECC
EGPPAPKAPQGVLAGKTVVLTGTLPSLAREEAKEMLEAAGAKVAGSVSKKTDYVVAGAEA
CCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCC
GSKLAKAEELGIPVLDEDGMRKLLEGQL
CHHHHHHHHCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA