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The map label for this gene is citZ [H]

Identifier: 91784081

GI number: 91784081

Start: 3005228

End: 3006442

Strand: Reverse

Name: citZ [H]

Synonym: Bxe_A1721

Alternate gene names: 91784081

Gene position: 3006442-3005228 (Counterclockwise)

Preceding gene: 91784092

Following gene: 91784080

Centisome position: 61.41

GC content: 62.39

Gene sequence:

>1215_bases
ATGACAAACACAACTCCCGTGCCGCTGGGCTCCAGCGATAGCCTTTACATGACGGCGGAGGAAGCCGCGAGCGCATTGAA
TGTCAGCGTGCCCACGCTCTATACCTATGTCAGCCGCAAGAACATCCGTGCGCACAAGACTCGCGGCAGCCGGGTCAGCC
GGTACCTGCGCTCGGATATTGAACAGATCAAGTCGGGGATCGCGCCTGCCTCGGCGCGCGACACCGCGCCCGGTCTGACG
GGCTCGTCGGCTATCACCTTGATGACGGAGGCGGGCTCCTTCTATCGCGGTGTGAGTGCCATCGATCTGGCAGAGAAGGC
CACTCTGGAGGACGTTGCCCGGCTGCTGTGGGATGCCGGTGACTTCGATCCCTTTGAATGCCCTGCGACCGCGCTTCCCC
CGCAAAGTGATGCGCTGCTCAAGGCCTGTGGCAACTTTAGTGCGCTGGACCGAGCCATCATGCTGCTTCCCGCCATCGAG
GCGGCGAATCCTCGTGCGCACGATCTCGGCAAGCATGGCTTTCTTCGCTCCGGGGCAGACGTGTTGCGCTGGTTCGCGGC
CTGGATGCTCGGACAGTCATCTCCGGCCAGGCTGCCGCTGCATCGCTACATTGGTGCGACGACAAAATGCGGACCGAAAC
TTGAGGATGCCGTACGCAGGGTGCTCGTTCTTTCGGCCGATCAGGCATTGCATCCCGCAACCTACGTCGCTCGCGCGGCG
GCCAGTACCGGCGCAACGCCCTATCGGTGCGTGATTGCTGGCCTGTCGGCTGCCACGGGCAAACGCTTGCCGGCTGTTCG
CGTCGGTGCGTTTTCCCGCTTTCTGGCCGAAATCGACAACGCCCAGGATTCGACCGAGCCGATCAGGATTCGCATGCGGG
AGAGCGAAGACATTCCTGGGTTTGGCTATTCGCCATTCAATGCGCCAGATCCACGTTCGGCAGCATTGTGGGCCTCGTTG
CGCGTTCTGCTGGCGACCGACCGGAACTTTGCACGCTTCGAGCGCGCTATCGACCTCGCCATTGATCTTACGGGCCAGCA
ACCGGATTTTGCGCTGCTGGCTGCCTATGTGAACCGCCGCATCGGCGCCGAGCCGGACGCGAATCTGGTGCGGCTGGGGC
GCATTGCCGGTTGGGTGGCACATGCGTGGGAGCAGCAAAACGAGATGCCCCTGGTACGCTGGAAGGTCAATTACAACGGT
GTTCTACCCGGTTGA

Upstream 100 bases:

>100_bases
GATGATCAATGGCCCGGCTGGGAACAAGTGTCGATGTATGTGGCATCATCAGTCAATCTCGATGATAATTGTCGATATAT
TGACTTGATCTCCGCCCCAC

Downstream 100 bases:

>100_bases
TTCTTATATCGATACAAACAATCGATATTGACACATTCCTTTCCCGAACTGTATGTTCAGCGAAAACCTGGGGTTCAAGG
AGACAGCGTGAACAAGCGGT

Product: excisionase/Xis, DNA-binding

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTNTTPVPLGSSDSLYMTAEEAASALNVSVPTLYTYVSRKNIRAHKTRGSRVSRYLRSDIEQIKSGIAPASARDTAPGLT
GSSAITLMTEAGSFYRGVSAIDLAEKATLEDVARLLWDAGDFDPFECPATALPPQSDALLKACGNFSALDRAIMLLPAIE
AANPRAHDLGKHGFLRSGADVLRWFAAWMLGQSSPARLPLHRYIGATTKCGPKLEDAVRRVLVLSADQALHPATYVARAA
ASTGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLAEIDNAQDSTEPIRIRMRESEDIPGFGYSPFNAPDPRSAALWASL
RVLLATDRNFARFERAIDLAIDLTGQQPDFALLAAYVNRRIGAEPDANLVRLGRIAGWVAHAWEQQNEMPLVRWKVNYNG
VLPG

Sequences:

>Translated_404_residues
MTNTTPVPLGSSDSLYMTAEEAASALNVSVPTLYTYVSRKNIRAHKTRGSRVSRYLRSDIEQIKSGIAPASARDTAPGLT
GSSAITLMTEAGSFYRGVSAIDLAEKATLEDVARLLWDAGDFDPFECPATALPPQSDALLKACGNFSALDRAIMLLPAIE
AANPRAHDLGKHGFLRSGADVLRWFAAWMLGQSSPARLPLHRYIGATTKCGPKLEDAVRRVLVLSADQALHPATYVARAA
ASTGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLAEIDNAQDSTEPIRIRMRESEDIPGFGYSPFNAPDPRSAALWASL
RVLLATDRNFARFERAIDLAIDLTGQQPDFALLAAYVNRRIGAEPDANLVRLGRIAGWVAHAWEQQNEMPLVRWKVNYNG
VLPG
>Mature_403_residues
TNTTPVPLGSSDSLYMTAEEAASALNVSVPTLYTYVSRKNIRAHKTRGSRVSRYLRSDIEQIKSGIAPASARDTAPGLTG
SSAITLMTEAGSFYRGVSAIDLAEKATLEDVARLLWDAGDFDPFECPATALPPQSDALLKACGNFSALDRAIMLLPAIEA
ANPRAHDLGKHGFLRSGADVLRWFAAWMLGQSSPARLPLHRYIGATTKCGPKLEDAVRRVLVLSADQALHPATYVARAAA
STGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLAEIDNAQDSTEPIRIRMRESEDIPGFGYSPFNAPDPRSAALWASLR
VLLATDRNFARFERAIDLAIDLTGQQPDFALLAAYVNRRIGAEPDANLVRLGRIAGWVAHAWEQQNEMPLVRWKVNYNGV
LPG

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 43674; Mature: 43543

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTTPVPLGSSDSLYMTAEEAASALNVSVPTLYTYVSRKNIRAHKTRGSRVSRYLRSDI
CCCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHH
EQIKSGIAPASARDTAPGLTGSSAITLMTEAGSFYRGVSAIDLAEKATLEDVARLLWDAG
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DFDPFECPATALPPQSDALLKACGNFSALDRAIMLLPAIEAANPRAHDLGKHGFLRSGAD
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHCCHH
VLRWFAAWMLGQSSPARLPLHRYIGATTKCGPKLEDAVRRVLVLSADQALHPATYVARAA
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCHHHHHHHHH
ASTGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLAEIDNAQDSTEPIRIRMRESEDIPG
HCCCCCCHHHHEECCHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
FGYSPFNAPDPRSAALWASLRVLLATDRNFARFERAIDLAIDLTGQQPDFALLAAYVNRR
CCCCCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHH
IGAEPDANLVRLGRIAGWVAHAWEQQNEMPLVRWKVNYNGVLPG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TNTTPVPLGSSDSLYMTAEEAASALNVSVPTLYTYVSRKNIRAHKTRGSRVSRYLRSDI
CCCCCCCCCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHH
EQIKSGIAPASARDTAPGLTGSSAITLMTEAGSFYRGVSAIDLAEKATLEDVARLLWDAG
HHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DFDPFECPATALPPQSDALLKACGNFSALDRAIMLLPAIEAANPRAHDLGKHGFLRSGAD
CCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHCCHH
VLRWFAAWMLGQSSPARLPLHRYIGATTKCGPKLEDAVRRVLVLSADQALHPATYVARAA
HHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCHHHHHHHHH
ASTGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLAEIDNAQDSTEPIRIRMRESEDIPG
HCCCCCCHHHHEECCHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
FGYSPFNAPDPRSAALWASLRVLLATDRNFARFERAIDLAIDLTGQQPDFALLAAYVNRR
CCCCCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHEEEEEECCCCCCHHHHHHHHHHH
IGAEPDANLVRLGRIAGWVAHAWEQQNEMPLVRWKVNYNGVLPG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]