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The map label for this gene is rfbI [H]

Identifier: 91783980

GI number: 91783980

Start: 2893503

End: 2894528

Strand: Reverse

Name: rfbI [H]

Synonym: Bxe_A1828

Alternate gene names: 91783980

Gene position: 2894528-2893503 (Counterclockwise)

Preceding gene: 91783981

Following gene: 91783979

Centisome position: 59.12

GC content: 61.5

Gene sequence:

>1026_bases
ATGGTCTATCAGATAGAAATGGCGGGAACCGGCGAGCGATTCGAAGCCCGTGCCGACGAATCGCTGCTGCAGGCGGCGAC
GAGTGCAGGGGTTGCAATTCCTCACGATTGCCAACTGGGCGGATGCGGAACGTGTCGCGTGAAGGTAATCAGCGGGACGC
ACGAATATGAAGAATTTCCGTTCGGCATCTCGCAGGAGGAAGCCGACGCCGGTTTTATGCTTGCCTGCCAGGCGCGGGCA
AGCAGCGACATGGTGATTGCCGCGGCGCACGTGGGCGGAGATGCATCGGCGCCTGCGGAGATCGAAGCGCGTGTGGTTGC
CGTCAGCCCGCTGGGGCCCGGAGTCCGGCATCTGTCGATCGAGCTGCCCGAGCAGGCGGAGCTCAGGTTCAATCCCGGCC
AATACATGAACGTTCTGCTCGGCGAAGGCGAACGCAGGAGCTTTTCGATGGCTTCGGCTGCCGCCGGCTCCGTGCTCGAT
TTTCATATCAGGCGGGTTCCAGGCGGCCGGTTTACGGATCACATGCTGGCGTCGCTGAAGCCGGGGGATCCGCTGAACGT
CGAGTTGCCGCTGGGCAGTTTCCGCTTTCATGTGGAGGACTATCGTCCGCTTCTGATGGTGGCGACGGGAACGGGACTCG
CGCCCGTCAAGGCGATTCTGGAATCGCTCATGGACGATCCGGACTGTCCGCCAGTGTGGCTCTACTGGGGCGCACGTACC
GAGGCGGATCTGTATCTGCACGACGAGATTCAGCAATGGGGTGCGCGTCTCTACGAGTTCCAGTACGTACCGGTGCTCTC
GCGTCCCGGCAGCGAATGGGCGGGACGACAGGGCTACGTCCAGCACGCGGTGTGCGAAGATTTTGCGGACCTGTCGGAAT
ACGCCGTCTACCTGTGCGGTTCTCCGACGATGATTAACGATGCCCGGCGTCACTTCCTGGAGAAAGGGGCAAGCATCAAT
CATCTGTACGCGGACAGTTTCACTTTTCAGCACGACGCCGAAGCGCTCGCAAACAGCGGAACGTGA

Upstream 100 bases:

>100_bases
GGCCAGCGGACGTCGCGTCCTCGTGGAAGCCGACATCAAGAAGTAATGCGGTCTGCCGCCCGGTGGCAGGCTGGTCCATT
GTTTCGTTAGAGATTGCAAG

Downstream 100 bases:

>100_bases
ACGTTGCCGACGGGTGACGGCTTCACTGGTGCCGTGGCCGTTGGCGTGCACCCGACCACCTTGGGCGACGTCCATGCAAA
ACGTCGTGCCACGCGAACCG

Product: putative CDP-6-deoxy-delta-3,4- glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MVYQIEMAGTGERFEARADESLLQAATSAGVAIPHDCQLGGCGTCRVKVISGTHEYEEFPFGISQEEADAGFMLACQARA
SSDMVIAAAHVGGDASAPAEIEARVVAVSPLGPGVRHLSIELPEQAELRFNPGQYMNVLLGEGERRSFSMASAAAGSVLD
FHIRRVPGGRFTDHMLASLKPGDPLNVELPLGSFRFHVEDYRPLLMVATGTGLAPVKAILESLMDDPDCPPVWLYWGART
EADLYLHDEIQQWGARLYEFQYVPVLSRPGSEWAGRQGYVQHAVCEDFADLSEYAVYLCGSPTMINDARRHFLEKGASIN
HLYADSFTFQHDAEALANSGT

Sequences:

>Translated_341_residues
MVYQIEMAGTGERFEARADESLLQAATSAGVAIPHDCQLGGCGTCRVKVISGTHEYEEFPFGISQEEADAGFMLACQARA
SSDMVIAAAHVGGDASAPAEIEARVVAVSPLGPGVRHLSIELPEQAELRFNPGQYMNVLLGEGERRSFSMASAAAGSVLD
FHIRRVPGGRFTDHMLASLKPGDPLNVELPLGSFRFHVEDYRPLLMVATGTGLAPVKAILESLMDDPDCPPVWLYWGART
EADLYLHDEIQQWGARLYEFQYVPVLSRPGSEWAGRQGYVQHAVCEDFADLSEYAVYLCGSPTMINDARRHFLEKGASIN
HLYADSFTFQHDAEALANSGT
>Mature_341_residues
MVYQIEMAGTGERFEARADESLLQAATSAGVAIPHDCQLGGCGTCRVKVISGTHEYEEFPFGISQEEADAGFMLACQARA
SSDMVIAAAHVGGDASAPAEIEARVVAVSPLGPGVRHLSIELPEQAELRFNPGQYMNVLLGEGERRSFSMASAAAGSVLD
FHIRRVPGGRFTDHMLASLKPGDPLNVELPLGSFRFHVEDYRPLLMVATGTGLAPVKAILESLMDDPDCPPVWLYWGART
EADLYLHDEIQQWGARLYEFQYVPVLSRPGSEWAGRQGYVQHAVCEDFADLSEYAVYLCGSPTMINDARRHFLEKGASIN
HLYADSFTFQHDAEALANSGT

Specific function: Catalyzes The Reduction Of Soluble Flavins By Reduced Pyridine Nucleotides. Seems To Reduces The Complexed Fe(3+) Iron Of Siderophores To Fe(2+), Thus Releasing It From The Chelator. [C]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI84875541, Length=174, Percent_Identity=30.4597701149425, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI2367314, Length=231, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322146, Length=184, Percent_Identity=28.2608695652174, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=186, Percent_Identity=27.4193548387097, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 37128; Mature: 37128

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYQIEMAGTGERFEARADESLLQAATSAGVAIPHDCQLGGCGTCRVKVISGTHEYEEFP
CEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHCC
FGISQEEADAGFMLACQARASSDMVIAAAHVGGDASAPAEIEARVVAVSPLGPGVRHLSI
CCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCEEEEE
ELPEQAELRFNPGQYMNVLLGEGERRSFSMASAAAGSVLDFHIRRVPGGRFTDHMLASLK
ECCCCCCEEECCCCEEEEEECCCCCCCEEHHHHHCCCEEEEEEEECCCCCCHHHHHHHCC
PGDPLNVELPLGSFRFHVEDYRPLLMVATGTGLAPVKAILESLMDDPDCPPVWLYWGART
CCCCCEEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
EADLYLHDEIQQWGARLYEFQYVPVLSRPGSEWAGRQGYVQHAVCEDFADLSEYAVYLCG
CCEEEEHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEC
SPTMINDARRHFLEKGASINHLYADSFTFQHDAEALANSGT
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MVYQIEMAGTGERFEARADESLLQAATSAGVAIPHDCQLGGCGTCRVKVISGTHEYEEFP
CEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCHHHCC
FGISQEEADAGFMLACQARASSDMVIAAAHVGGDASAPAEIEARVVAVSPLGPGVRHLSI
CCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEEEEEEECCCCCCEEEEE
ELPEQAELRFNPGQYMNVLLGEGERRSFSMASAAAGSVLDFHIRRVPGGRFTDHMLASLK
ECCCCCCEEECCCCEEEEEECCCCCCCEEHHHHHCCCEEEEEEEECCCCCCHHHHHHHCC
PGDPLNVELPLGSFRFHVEDYRPLLMVATGTGLAPVKAILESLMDDPDCPPVWLYWGART
CCCCCEEEECCCCEEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
EADLYLHDEIQQWGARLYEFQYVPVLSRPGSEWAGRQGYVQHAVCEDFADLSEYAVYLCG
CCEEEEHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEC
SPTMINDARRHFLEKGASINHLYADSFTFQHDAEALANSGT
CCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]