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The map label for this gene is bcr [H]

Identifier: 91783976

GI number: 91783976

Start: 2888776

End: 2890038

Strand: Direct

Name: bcr [H]

Synonym: Bxe_A1832

Alternate gene names: 91783976

Gene position: 2888776-2890038 (Clockwise)

Preceding gene: 91783975

Following gene: 91783984

Centisome position: 59.0

GC content: 62.39

Gene sequence:

>1263_bases
ATGAACCACACTATCACCGACTGTTCCACTTCTACCGCGCGCCCACCGAACTCGCCGCGCTTCCTGCTGTTCCTGATCTG
CCTGTTCGCTTCGGCCGGACAACTCGCAATCGACATCTACGTACCCGCGCTACCCGCGATGGCGCATTTCTTTGCGACAT
CCCCTCAGGCGATCCAGTCGAGCGTGTCCGGATACATGGTGGCCTATGCGCTCGGCCAACTCGTCTTCGGACCGGTGGCC
GACGCTTATGGACGCAAACGCGTGCTGGCTTTCGGGCTCGTGATCTACACGCTTGGCTGCCTGCTGTCACTCGTCGCATC
GAATCTGGAGACATTCGTTCTTGCCCGCTGTCTGCAAGGATTCGGCATCGCTACCACGAACCTGCTCGCGAAAGCGATCA
TCACCGATTCGTTCGCCGGACAAGCGCTGGTGCATGCGTTCACCTATATGTCGATCGCGTGGGGACTCGCGCCGATCGTG
GCGCCGGTGATCGGCGCGCATCTGCAAACGTGGTTTGGCTGGCAAGCCTGCCTCGTCTTTCTGCTGGTTTATTCGCTGGT
GATGTGGGGACTGCTGTGGCGCTACCGCGAAACATTGGTGCAGCCGGTGCATCTCGCGCCGCGCACGCTGATGGCGAATG
CGGGCAAGGTGCTGTCGAGCCCGGTGTTCCAGAGCTGCTTCCTCGCGCAAGGCCTGTGCTACAGCATCCTGCTCGTGTTC
AACATCGTCGGGCCCTTCATGGTGCAGAACACGTTGCACAAACCGCCGACTTTTTTCGGCTACCTCGCGCTCGGCATCGG
GGCCATGTACTTCCTCGGCGGGCTTTCGAATCGTGTGCACGGCGCACGTCTGCCGGATGCGGAACAGCGGCTGCGCATCG
GCGCGCGCGTGATGGCGGCCGCGTCGATTGCGATGCTGGTTCTGGCGCTGACAGTCGGCCTGCGAGTGTGGACACTCGCG
CTGCCCGTGCTGGTGATGGGGTTCTGCGCGGGTGCGATGTATCCGACTCTGATGGCCAAGGGAAATTCGCTGTTCCCGCA
CATCGCGGGCCTGACGAGCGCGATCCTCGGGTGTGCGCTGCTGCTGGTCTCGTCGGCGATGATGGGACTGGCCGGCTTCG
TATCGGTCCAGGAACTGACGTCGCTGGCGGTGTTTTTTGTGATCCTCGCCCTTACGGTCGTGGCGATGGTGACTAAGCTG
CTACGCCATCTGGCGCAACCTCAGTCCGCAGCCGTGGCACGCGGGAGCGGCGAAGCGGTGTAA

Upstream 100 bases:

>100_bases
CGCAGCGACCCCGTGCGCCCATGCTCCAGGTATCGCCGCCGCGACGGCCAGGACGATCGCGCCTGTTCCCGTCACCAGAA
TCTGTTCCCTTTATGCTGTC

Downstream 100 bases:

>100_bases
GTACGCTTCTGCTTCCGAATTCGCCGCACGTCAAAACGGAGTGTATGGAAACGACGTGCGGTCCTGAGTAACGAGGGCTC
GTGAGGTTCCGCCAGACCGT

Product: major facilitator superfamily drug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNHTITDCSTSTARPPNSPRFLLFLICLFASAGQLAIDIYVPALPAMAHFFATSPQAIQSSVSGYMVAYALGQLVFGPVA
DAYGRKRVLAFGLVIYTLGCLLSLVASNLETFVLARCLQGFGIATTNLLAKAIITDSFAGQALVHAFTYMSIAWGLAPIV
APVIGAHLQTWFGWQACLVFLLVYSLVMWGLLWRYRETLVQPVHLAPRTLMANAGKVLSSPVFQSCFLAQGLCYSILLVF
NIVGPFMVQNTLHKPPTFFGYLALGIGAMYFLGGLSNRVHGARLPDAEQRLRIGARVMAAASIAMLVLALTVGLRVWTLA
LPVLVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSSAMMGLAGFVSVQELTSLAVFFVILALTVVAMVTKL
LRHLAQPQSAAVARGSGEAV

Sequences:

>Translated_420_residues
MNHTITDCSTSTARPPNSPRFLLFLICLFASAGQLAIDIYVPALPAMAHFFATSPQAIQSSVSGYMVAYALGQLVFGPVA
DAYGRKRVLAFGLVIYTLGCLLSLVASNLETFVLARCLQGFGIATTNLLAKAIITDSFAGQALVHAFTYMSIAWGLAPIV
APVIGAHLQTWFGWQACLVFLLVYSLVMWGLLWRYRETLVQPVHLAPRTLMANAGKVLSSPVFQSCFLAQGLCYSILLVF
NIVGPFMVQNTLHKPPTFFGYLALGIGAMYFLGGLSNRVHGARLPDAEQRLRIGARVMAAASIAMLVLALTVGLRVWTLA
LPVLVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSSAMMGLAGFVSVQELTSLAVFFVILALTVVAMVTKL
LRHLAQPQSAAVARGSGEAV
>Mature_420_residues
MNHTITDCSTSTARPPNSPRFLLFLICLFASAGQLAIDIYVPALPAMAHFFATSPQAIQSSVSGYMVAYALGQLVFGPVA
DAYGRKRVLAFGLVIYTLGCLLSLVASNLETFVLARCLQGFGIATTNLLAKAIITDSFAGQALVHAFTYMSIAWGLAPIV
APVIGAHLQTWFGWQACLVFLLVYSLVMWGLLWRYRETLVQPVHLAPRTLMANAGKVLSSPVFQSCFLAQGLCYSILLVF
NIVGPFMVQNTLHKPPTFFGYLALGIGAMYFLGGLSNRVHGARLPDAEQRLRIGARVMAAASIAMLVLALTVGLRVWTLA
LPVLVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSSAMMGLAGFVSVQELTSLAVFFVILALTVVAMVTKL
LRHLAQPQSAAVARGSGEAV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=355, Percent_Identity=25.9154929577465, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI48994889, Length=345, Percent_Identity=27.536231884058, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082312, Length=360, Percent_Identity=26.6666666666667, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1790195, Length=155, Percent_Identity=29.6774193548387, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787065, Length=157, Percent_Identity=28.0254777070064, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790146, Length=240, Percent_Identity=26.25, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44767; Mature: 44767

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]