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The map label for this gene is pucI [H]

Identifier: 91783887

GI number: 91783887

Start: 2805071

End: 2806552

Strand: Reverse

Name: pucI [H]

Synonym: Bxe_A1924

Alternate gene names: 91783887

Gene position: 2806552-2805071 (Counterclockwise)

Preceding gene: 91783889

Following gene: 91783886

Centisome position: 57.33

GC content: 62.15

Gene sequence:

>1482_bases
ATGGCTCAGTTCAGTGCAGCGCCCGGCAGTCCCGCAATTCCCGCTTACGCCGACGGCGGCGCGCCCGGCGATCCGTCGAT
GCCGGCAGGCTACAGCGAGCGGCTCTATAACGAAGACCTCGCGCCGTTGCGCCATCAAACCTGGGGCGCCTACAACATCT
TCGCGTTCTGGATGTCGGACGTGCACAGCGTCGGCGGCTATGTGTTCGCGGGCAGTCTGTTCGCGCTCGGCCTGACGAGC
TGGCAGGTGCTGATCGCGCTGCTGGTCGGCATCACGATCGTGAACCTCTTGTGCAACCTGATCGCGAAGCCCAGCCAGGC
GAACGGCGTACCTTATCCGGTCGCATGCCGCGCGACTTTCGGCGTGCTCGGCGCGAATATTCCCGCGGTGATTCGCGGCC
TGATCGCGGTTGCGTGGTATGGCATTCAGACCTATCTGGCGTCGAGCGCGCTGGTGATCGTGGTGTTGAAGTTCGTTCCG
CAGTTGCTGCCTTATGCCGATGTTCATCATCACGGCTTCATGGGGCTCTCCACGCTCGGCTGGGCCGGTTTCATGCTGCT
GTGGGTCTTGCAGGCACTCGTGTTCTGGCACGGCATGGAGACCATCAAGAAGTTCATCGACTTTGCCGGGCCGGCCGTCT
ACGTGGTGATGTTCATTCTCGCGGGCTACATGGTGTATCGCGCAGGGTGGCGCAACATCGGCATCAATCTGGGCGGTGTC
AAATATCACGGCATGCAGGTCGTGCCGGTGATGATCACGGCGACTTCGCTGGTGGTGTCGTACTTCTCCGGACCCATGCT
GAACTTCGGCGACTTTTCGCGCTACGGCAAGAGCTTTCGCAGCGTGCAGCGTGGCAACTTCTGGGGGCTGCCGGTCAACT
TTCTGGCCTTCTCGCTGGTGACTGTGATCACGACCGCGGCCACGCTGCCGGTGTTCGGCGAGCTGATCACGGACCCGGTC
GAAACCGTGGGCCGTATCGACTACCCGACCGCGGTGATTCTCGGCGCGCTGACCTTCACGATCGCGACGATCGGCATCAA
CATCGTCGCCAATTTCGTCTCGCCCGCGTTCGACTTTTCCAATGTCGCGCCGCGCCTGATCAGCTGGCGCGCGGGCGGTA
TGCTCGCGGCGGTGGCGTCGATCTTCATCACGCCGTGGAATCTGTTCAACAACCCGGCCGTGATTCACTACACGCTGGAC
GTTCTCGGCAGCTTCATCGGACCTTTGTACGGTGTACTGATCGTCGATTACTACCTCGTGAAGCGTCAGAGAATCGTTCT
CGACGATCTGTACACGGTCGCGCCCACCGGCTCGTACTGGTATCGCAACGGCGTGAACTATCGGGCCGTGGCCGCGCTGC
TGCCGGCAGCCGTGATCGCGGTGATCTGCGTGATGGTGCCCTCGCTCGACGGTATGGCGAACTTCTCGTGGTTCATCGGC
GCCGGGCTCGCCGCGCTGTTCTATCGCGTGATTGCACGCTGA

Upstream 100 bases:

>100_bases
GCATGTCCTTTGTTCCGAGCGGACAGAGCGCGCGCGACTGGCATGGCCGATGCAACGTCCTTCGATTCGCTATCGATGGT
CCCTATCCCAAGGAGGAATC

Downstream 100 bases:

>100_bases
ATTTTGCAGATGCAACAGGAGTCGATATGCGCATCAAACTGATCAACCCGAATACCACGCAACGCATGACGGAGGCGATG
GGCCGCTGCGCGCGTGAAGT

Product: NCS1 family nucleobase/cation symporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MAQFSAAPGSPAIPAYADGGAPGDPSMPAGYSERLYNEDLAPLRHQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTS
WQVLIALLVGITIVNLLCNLIAKPSQANGVPYPVACRATFGVLGANIPAVIRGLIAVAWYGIQTYLASSALVIVVLKFVP
QLLPYADVHHHGFMGLSTLGWAGFMLLWVLQALVFWHGMETIKKFIDFAGPAVYVVMFILAGYMVYRAGWRNIGINLGGV
KYHGMQVVPVMITATSLVVSYFSGPMLNFGDFSRYGKSFRSVQRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPV
ETVGRIDYPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLD
VLGSFIGPLYGVLIVDYYLVKRQRIVLDDLYTVAPTGSYWYRNGVNYRAVAALLPAAVIAVICVMVPSLDGMANFSWFIG
AGLAALFYRVIAR

Sequences:

>Translated_493_residues
MAQFSAAPGSPAIPAYADGGAPGDPSMPAGYSERLYNEDLAPLRHQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTS
WQVLIALLVGITIVNLLCNLIAKPSQANGVPYPVACRATFGVLGANIPAVIRGLIAVAWYGIQTYLASSALVIVVLKFVP
QLLPYADVHHHGFMGLSTLGWAGFMLLWVLQALVFWHGMETIKKFIDFAGPAVYVVMFILAGYMVYRAGWRNIGINLGGV
KYHGMQVVPVMITATSLVVSYFSGPMLNFGDFSRYGKSFRSVQRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPV
ETVGRIDYPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLD
VLGSFIGPLYGVLIVDYYLVKRQRIVLDDLYTVAPTGSYWYRNGVNYRAVAALLPAAVIAVICVMVPSLDGMANFSWFIG
AGLAALFYRVIAR
>Mature_492_residues
AQFSAAPGSPAIPAYADGGAPGDPSMPAGYSERLYNEDLAPLRHQTWGAYNIFAFWMSDVHSVGGYVFAGSLFALGLTSW
QVLIALLVGITIVNLLCNLIAKPSQANGVPYPVACRATFGVLGANIPAVIRGLIAVAWYGIQTYLASSALVIVVLKFVPQ
LLPYADVHHHGFMGLSTLGWAGFMLLWVLQALVFWHGMETIKKFIDFAGPAVYVVMFILAGYMVYRAGWRNIGINLGGVK
YHGMQVVPVMITATSLVVSYFSGPMLNFGDFSRYGKSFRSVQRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVE
TVGRIDYPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDV
LGSFIGPLYGVLIVDYYLVKRQRIVLDDLYTVAPTGSYWYRNGVNYRAVAALLPAAVIAVICVMVPSLDGMANFSWFIGA
GLAALFYRVIAR

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=470, Percent_Identity=28.0851063829787, Blast_Score=191, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6319495, Length=448, Percent_Identity=25.4464285714286, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6322219, Length=452, Percent_Identity=24.1150442477876, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319429, Length=483, Percent_Identity=24.6376811594203, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323266, Length=472, Percent_Identity=22.8813559322034, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324766, Length=472, Percent_Identity=21.8220338983051, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324645, Length=474, Percent_Identity=21.9409282700422, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53366; Mature: 53235

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCC
>Mature Secondary Structure 
AQFSAAPGSPAIPAYADGGAPGDPSMPAGYSERLYNEDLAPLRHQTWGAYNIFAFWMSD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHCCCHHHHHHHHHHH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HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]