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The map label for this gene is oprM [H]

Identifier: 91783846

GI number: 91783846

Start: 2756496

End: 2757992

Strand: Direct

Name: oprM [H]

Synonym: Bxe_A1966

Alternate gene names: 91783846

Gene position: 2756496-2757992 (Clockwise)

Preceding gene: 91783845

Following gene: 91783851

Centisome position: 56.3

GC content: 69.41

Gene sequence:

>1497_bases
ATGACCACGCCCACACTCTTCCGATACTCGCGCATCACCGCGCTCGCCGGCATGGCATGCGCGCTCGCCGCCTGCTCGTT
CGGGCCGGGCGGCGAGCCGCCCGCCATGCCGCAACCCGCGCACTACGGCGCCGAAGCGCAGCCGGCGCAAACCGTGCCCG
CGCAAGGCGTCACCCAGCAGTTCGTGGTGGGCGCGAAGCCGGTGCCCGAATGGTGGAAGCTGTATCAGTCGGACGCGCTG
AACGCGCTGGTCGACGAAGGCTTGCGCAGCAGCCCCACCCTCGCCGCGACCGACAGGAGCCTCGCCGCCGCGCGCGAGCA
GTTGCGCGCGCAGATCGGCAGCTCGATGCTGCCCACCATCGACGCCGGCGGCCAGGCGACCCGCAATCGCGCGCTGGCGA
TCCCTGAGATCGGACCCAACACGTTTCTGTACAACGTCTTTGTCGGTCAATTGCAGGCGCACTACACGTTCGACCTGTTC
GGTGCGGCGCGCCTCGCCGATGCCGCGCTCGCCGCGCGCGTCAACGTTCAGGCCTATCAGTTCGACGCCGCGCGCCGCGC
TCTGGCCGCCAACATCGTGACCGCGGCGATCACCAGCGCGGCGCTGCATGCGCAGATCGAGACGACCGAACGGCTCGTGA
CGATCGCCAACGATCAGGCACGCGACACGCAGCGGCGTTATGCGCTGGGCGCGGTCTCGCATGCGGATCAGCTGAGCGCG
CAACAAAGCGCGGCCAGCTTGTCGGCGAGCTTGCCGGGCCTGAAGCAGCAATGGCTGAGCACGCGCCACGCGCTGGCGGT
GCTACTCGGCCGGACGCCTGACGCGGCGCCGGAGGATCTGGCGCTCGCGCAACTGCACGTGCCTGAGCAGGTGCCGGTGG
TGGTGCCGTCGGAACTGCTGCGCAGCCGCCCCGACATTCAGGCCGCCGACGCTGCGTTGAAGGCCGCGGCGGCCGATGTC
GGCGTCGCCACGGCGCAGATGTATCCGAGTCTGTCGTTGAGTGCATCGATGGGACAAGGCGGCTTTAGCTGGCCGGTCGC
GCTCTCCGGTGCGGGCGCGATCTGGAGCATCGGTGCGTCGCTGTCGCAGCCGTTGTTTCATGGCGGCGCACTGGTCGCGC
AACGGCGTGCGGCGCTCGATACCTATGACGCCACGGTCTCGCAGTACAAGCAAACGGTATTGGCCGCGTTCCAGAACGTC
GCGGACACGCTTGCGGCGCTCGAGCACGACTCGCAGGCGCTGGACGCCGCGAATATTGCGGCGCGCTCGGCGCAAGGCAT
CTTCGATGAAACCTCCGGGCGCTACCGGCTGGGCGCGCTGCCTGTGGCGTCGGCGCGTTCGAGCGAGCAGCAGTTCCGCA
ACGCGCAGCTTGATGAGATTCGCTATACGAGCGCGCGGCTGACGGACACGGCGGCGCTGTTCCAGGCGATGGGGAATCCG
CCGGTTGAGACGGGGGTGGGTGGGGCTTCGGCGGCGAGCGCTGGCGTCGCCGAGTAG

Upstream 100 bases:

>100_bases
ATCTGCCCGCGCTGTACGCCACGTCGTTCCGCGTGCGTGCCGATGAACGCGAGCCGCAGACGCCGTCCGCGTCCGGCACG
ACGCATACGGGGAATTGAGC

Downstream 100 bases:

>100_bases
CTAAACCTGGGATTCCATACGGATGGCCTGGAAGTTATCCCCACTTTTTGTGCACAAGTCCGTGGATAAGTCTCTCCCCA
CAGCGCTGAACCCATGCCAT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTTPTLFRYSRITALAGMACALAACSFGPGGEPPAMPQPAHYGAEAQPAQTVPAQGVTQQFVVGAKPVPEWWKLYQSDAL
NALVDEGLRSSPTLAATDRSLAAAREQLRAQIGSSMLPTIDAGGQATRNRALAIPEIGPNTFLYNVFVGQLQAHYTFDLF
GAARLADAALAARVNVQAYQFDAARRALAANIVTAAITSAALHAQIETTERLVTIANDQARDTQRRYALGAVSHADQLSA
QQSAASLSASLPGLKQQWLSTRHALAVLLGRTPDAAPEDLALAQLHVPEQVPVVVPSELLRSRPDIQAADAALKAAAADV
GVATAQMYPSLSLSASMGQGGFSWPVALSGAGAIWSIGASLSQPLFHGGALVAQRRAALDTYDATVSQYKQTVLAAFQNV
ADTLAALEHDSQALDAANIAARSAQGIFDETSGRYRLGALPVASARSSEQQFRNAQLDEIRYTSARLTDTAALFQAMGNP
PVETGVGGASAASAGVAE

Sequences:

>Translated_498_residues
MTTPTLFRYSRITALAGMACALAACSFGPGGEPPAMPQPAHYGAEAQPAQTVPAQGVTQQFVVGAKPVPEWWKLYQSDAL
NALVDEGLRSSPTLAATDRSLAAAREQLRAQIGSSMLPTIDAGGQATRNRALAIPEIGPNTFLYNVFVGQLQAHYTFDLF
GAARLADAALAARVNVQAYQFDAARRALAANIVTAAITSAALHAQIETTERLVTIANDQARDTQRRYALGAVSHADQLSA
QQSAASLSASLPGLKQQWLSTRHALAVLLGRTPDAAPEDLALAQLHVPEQVPVVVPSELLRSRPDIQAADAALKAAAADV
GVATAQMYPSLSLSASMGQGGFSWPVALSGAGAIWSIGASLSQPLFHGGALVAQRRAALDTYDATVSQYKQTVLAAFQNV
ADTLAALEHDSQALDAANIAARSAQGIFDETSGRYRLGALPVASARSSEQQFRNAQLDEIRYTSARLTDTAALFQAMGNP
PVETGVGGASAASAGVAE
>Mature_497_residues
TTPTLFRYSRITALAGMACALAACSFGPGGEPPAMPQPAHYGAEAQPAQTVPAQGVTQQFVVGAKPVPEWWKLYQSDALN
ALVDEGLRSSPTLAATDRSLAAAREQLRAQIGSSMLPTIDAGGQATRNRALAIPEIGPNTFLYNVFVGQLQAHYTFDLFG
AARLADAALAARVNVQAYQFDAARRALAANIVTAAITSAALHAQIETTERLVTIANDQARDTQRRYALGAVSHADQLSAQ
QSAASLSASLPGLKQQWLSTRHALAVLLGRTPDAAPEDLALAQLHVPEQVPVVVPSELLRSRPDIQAADAALKAAAADVG
VATAQMYPSLSLSASMGQGGFSWPVALSGAGAIWSIGASLSQPLFHGGALVAQRRAALDTYDATVSQYKQTVLAAFQNVA
DTLAALEHDSQALDAANIAARSAQGIFDETSGRYRLGALPVASARSSEQQFRNAQLDEIRYTSARLTDTAALFQAMGNPP
VETGVGGASAASAGVAE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=23.0125523012552, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI2367348, Length=353, Percent_Identity=24.6458923512748, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51907; Mature: 51776

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPTLFRYSRITALAGMACALAACSFGPGGEPPAMPQPAHYGAEAQPAQTVPAQGVTQQ
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
FVVGAKPVPEWWKLYQSDALNALVDEGLRSSPTLAATDRSLAAAREQLRAQIGSSMLPTI
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCC
DAGGQATRNRALAIPEIGPNTFLYNVFVGQLQAHYTFDLFGAARLADAALAARVNVQAYQ
CCCCCHHCCCEEECCCCCCCCEEEHHHHHHHHHHEEEEHHHHHHHHHHHHHHHCCEEHHH
FDAARRALAANIVTAAITSAALHAQIETTERLVTIANDQARDTQRRYALGAVSHADQLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHH
QQSAASLSASLPGLKQQWLSTRHALAVLLGRTPDAAPEDLALAQLHVPEQVPVVVPSELL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCEECCHHHH
RSRPDIQAADAALKAAAADVGVATAQMYPSLSLSASMGQGGFSWPVALSGAGAIWSIGAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHCHH
LSQPLFHGGALVAQRRAALDTYDATVSQYKQTVLAAFQNVADTLAALEHDSQALDAANIA
HCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ARSAQGIFDETSGRYRLGALPVASARSSEQQFRNAQLDEIRYTSARLTDTAALFQAMGNP
HHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
PVETGVGGASAASAGVAE
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPTLFRYSRITALAGMACALAACSFGPGGEPPAMPQPAHYGAEAQPAQTVPAQGVTQQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
FVVGAKPVPEWWKLYQSDALNALVDEGLRSSPTLAATDRSLAAAREQLRAQIGSSMLPTI
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCC
DAGGQATRNRALAIPEIGPNTFLYNVFVGQLQAHYTFDLFGAARLADAALAARVNVQAYQ
CCCCCHHCCCEEECCCCCCCCEEEHHHHHHHHHHEEEEHHHHHHHHHHHHHHHCCEEHHH
FDAARRALAANIVTAAITSAALHAQIETTERLVTIANDQARDTQRRYALGAVSHADQLSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHH
QQSAASLSASLPGLKQQWLSTRHALAVLLGRTPDAAPEDLALAQLHVPEQVPVVVPSELL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHEECCCCCCCEECCHHHH
RSRPDIQAADAALKAAAADVGVATAQMYPSLSLSASMGQGGFSWPVALSGAGAIWSIGAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHCHH
LSQPLFHGGALVAQRRAALDTYDATVSQYKQTVLAAFQNVADTLAALEHDSQALDAANIA
HCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ARSAQGIFDETSGRYRLGALPVASARSSEQQFRNAQLDEIRYTSARLTDTAALFQAMGNP
HHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
PVETGVGGASAASAGVAE
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]