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The map label for this gene is tycC [H]

Identifier: 91783776

GI number: 91783776

Start: 2675147

End: 2676772

Strand: Reverse

Name: tycC [H]

Synonym: Bxe_A2036

Alternate gene names: 91783776

Gene position: 2676772-2675147 (Counterclockwise)

Preceding gene: 91783778

Following gene: 91783775

Centisome position: 54.67

GC content: 64.02

Gene sequence:

>1626_bases
ATGCTGGATTCATTCTTCTTCGAGACCGCCGCGCGTGAGCCGGATCGTCCCGCGCTATGGGTGGACGAGCGCTCGTATTG
CTACGACGAACTTGCTTCGCGAGCGGGCCGGATCGCGGCCGACGTATCTGCGGTGCTGCCGGCCGGCCGCGCGCGGCGCT
GCCTGCTATTCGCGCATCGCAGCGTGGACGCTTACGCGGGTTTGCTAGGAATCCTCAACGCAGACTGCGCTTATGTACCG
CTCAATCCCAACATGCCTGCGGCGCGTATTGCCGCCATAATCGAACAGTCGGACGCACCGGTCATGCTGGTTGACCGGCG
TTGCGCCGCGCTGCTCGACGAGGTGGTGTCGCTGCTGGACGAATCGCCCAGGATCTTCCTGCTCGACGACGAAGAGGGCG
AAGTCCAACAAGTCTCTCAGGACTCCGACGAAACGGCTCCGACCCGTGAAGTGTCGGCCGGCCGCGACCTGACACGGCTG
CCAAGCGATCCGCTTGTGGCCGAAACCGTCATGCCTCGCGCGACGTGCGACGTGGCCTATATTCTGTTCACGTCAGGTTC
GACCGGCGCACCCAAGGGCGTGCCCATCTCGCACGGGAATGCTTGTGCTTACGTGGCGGGGCAGTTGCATCTGATCGACA
GGCTGCCGGGTGCCCGCTATATACAGTTATGCGAGCTTTCCTTCGATCCTTCGGTACACGACATGTTCGTGTGCTGGGCA
AATGGCGCATGCCTGTACGTGCCGAAGACCGTCGAGCCGATCTACAACGCGGCCTTCATCAAGGAACACGCGATCACGCA
CTGGAGCAGCGTGCCTTCGGGCGCCGCCTTCATGCAGCAGTTCCGCAAGCTGCAGCCGGACGAATTTCCCAGCCTGCGCG
TGACGTTGTTCGGCGGAGAACCGTTGCCGCGTTTGCTGGTGCAAGCCTGGCTGCGCGCCGCGCCGAACAGCCGGGTGCTG
AACATGTACGGGCCGACTGAAACGACGGTTGCGTGCACCGCATTCGAAGTCACCACGGAATTTCTCGCCGATCCGCGCCG
CGCGGTCATGCCGCTTGGCCGCGCGTTGCCGGGCATGGAACTGTTGATCGTGGACGCCGCGCTCGAACCGGTCGCACCGG
GCATCAGCGGCGAATTGCTGATTGGCGGTCCGCAGGTCGCTTCGGGTTATCTGTCGGCGGACGAGCCCGGCAATCGCCGT
TTCATTTCGCGGATGTATCCGGGCCACAGCGCGTCGCGCTGGTATCGGAGCGCGGACGCCGCGCGCGAGGTGGCCGGCGA
GGGCATCGTGTTTCAAGGCCGCCTGGATACGCAGATCAAGATTCGCGGCAATCGTATCGAGCTCGAAGAAGTCGAGCATG
TCGTCAGGTCGTGCAGTCAGGCCGCGCTGTGCGCGGTTATCGCGTGGCCTGTGGATGAAGCGGGCCGCGCGGGTGGCCTG
ATCGCTTTCGTGACGCAGGTGCAGGGCGGCGCGACGCAGATTCTGCAGGCGTGTCGTCAGCGCTTGCCCGCGTACGCCGT
GCCGCAACGGGTCGTGATGCTGGACACGTTGCCGCTGAACGTCAATGGCAAGGTCGATAGAAGGGCGCTGGCCGGACAGT
GTATGACCGGCGATGTATTCACCTGA

Upstream 100 bases:

>100_bases
GCCCGGCAGCGTGCTGCCGGCATGGCTGGCAGCCACTCCGGCGCGCTCGAGCGACCGGTCCGGCGAGGCCGGCACGTTGC
CCCATGCGTCGAGGCCGTGA

Downstream 100 bases:

>100_bases
GCATCCCGCGGCGTGTCTCGTTGCACGTTCCTGACGGATCGGTGCCGTGTTGAAAAATCGAAGGATCGGGGGAATCACAA
TGAAGACGCATGTACGCCGT

Product: non-ribosomal peptide synthase amino acid adenylation subunit

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Tyrocidine synthase III; ATP-dependent asparagine adenylase; AsnA; Asparagine activase; ATP-dependent glutamine adenylase; GlnA; Glutamine activase; ATP-dependent tyrosine adenylase; TyrA; Tyrosine activase; ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent ornithine adenylase; OrnA; Ornithine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLDSFFFETAAREPDRPALWVDERSYCYDELASRAGRIAADVSAVLPAGRARRCLLFAHRSVDAYAGLLGILNADCAYVP
LNPNMPAARIAAIIEQSDAPVMLVDRRCAALLDEVVSLLDESPRIFLLDDEEGEVQQVSQDSDETAPTREVSAGRDLTRL
PSDPLVAETVMPRATCDVAYILFTSGSTGAPKGVPISHGNACAYVAGQLHLIDRLPGARYIQLCELSFDPSVHDMFVCWA
NGACLYVPKTVEPIYNAAFIKEHAITHWSSVPSGAAFMQQFRKLQPDEFPSLRVTLFGGEPLPRLLVQAWLRAAPNSRVL
NMYGPTETTVACTAFEVTTEFLADPRRAVMPLGRALPGMELLIVDAALEPVAPGISGELLIGGPQVASGYLSADEPGNRR
FISRMYPGHSASRWYRSADAAREVAGEGIVFQGRLDTQIKIRGNRIELEEVEHVVRSCSQAALCAVIAWPVDEAGRAGGL
IAFVTQVQGGATQILQACRQRLPAYAVPQRVVMLDTLPLNVNGKVDRRALAGQCMTGDVFT

Sequences:

>Translated_541_residues
MLDSFFFETAAREPDRPALWVDERSYCYDELASRAGRIAADVSAVLPAGRARRCLLFAHRSVDAYAGLLGILNADCAYVP
LNPNMPAARIAAIIEQSDAPVMLVDRRCAALLDEVVSLLDESPRIFLLDDEEGEVQQVSQDSDETAPTREVSAGRDLTRL
PSDPLVAETVMPRATCDVAYILFTSGSTGAPKGVPISHGNACAYVAGQLHLIDRLPGARYIQLCELSFDPSVHDMFVCWA
NGACLYVPKTVEPIYNAAFIKEHAITHWSSVPSGAAFMQQFRKLQPDEFPSLRVTLFGGEPLPRLLVQAWLRAAPNSRVL
NMYGPTETTVACTAFEVTTEFLADPRRAVMPLGRALPGMELLIVDAALEPVAPGISGELLIGGPQVASGYLSADEPGNRR
FISRMYPGHSASRWYRSADAAREVAGEGIVFQGRLDTQIKIRGNRIELEEVEHVVRSCSQAALCAVIAWPVDEAGRAGGL
IAFVTQVQGGATQILQACRQRLPAYAVPQRVVMLDTLPLNVNGKVDRRALAGQCMTGDVFT
>Mature_541_residues
MLDSFFFETAAREPDRPALWVDERSYCYDELASRAGRIAADVSAVLPAGRARRCLLFAHRSVDAYAGLLGILNADCAYVP
LNPNMPAARIAAIIEQSDAPVMLVDRRCAALLDEVVSLLDESPRIFLLDDEEGEVQQVSQDSDETAPTREVSAGRDLTRL
PSDPLVAETVMPRATCDVAYILFTSGSTGAPKGVPISHGNACAYVAGQLHLIDRLPGARYIQLCELSFDPSVHDMFVCWA
NGACLYVPKTVEPIYNAAFIKEHAITHWSSVPSGAAFMQQFRKLQPDEFPSLRVTLFGGEPLPRLLVQAWLRAAPNSRVL
NMYGPTETTVACTAFEVTTEFLADPRRAVMPLGRALPGMELLIVDAALEPVAPGISGELLIGGPQVASGYLSADEPGNRR
FISRMYPGHSASRWYRSADAAREVAGEGIVFQGRLDTQIKIRGNRIELEEVEHVVRSCSQAALCAVIAWPVDEAGRAGGL
IAFVTQVQGGATQILQACRQRLPAYAVPQRVVMLDTLPLNVNGKVDRRALAGQCMTGDVFT

Specific function: Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=558, Percent_Identity=24.7311827956989, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI28416953, Length=390, Percent_Identity=25.3846153846154, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI38505220, Length=561, Percent_Identity=23.5294117647059, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786801, Length=548, Percent_Identity=30.8394160583942, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1789201, Length=385, Percent_Identity=28.3116883116883, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788107, Length=554, Percent_Identity=22.7436823104693, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI145693145, Length=423, Percent_Identity=24.3498817966903, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786810, Length=442, Percent_Identity=23.7556561085973, Blast_Score=73, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556356, Length=311, Percent_Identity=29.5819935691318, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=361, Percent_Identity=27.7008310249307, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=454, Percent_Identity=24.0088105726872, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17550940, Length=353, Percent_Identity=21.8130311614731, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319591, Length=602, Percent_Identity=26.2458471760797, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=23.0555555555556, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648676, Length=489, Percent_Identity=27.6073619631902, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI18859661, Length=365, Percent_Identity=27.9452054794521, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 58725; Mature: 58725

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSFFFETAAREPDRPALWVDERSYCYDELASRAGRIAADVSAVLPAGRARRCLLFAHR
CCCHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECC
SVDAYAGLLGILNADCAYVPLNPNMPAARIAAIIEQSDAPVMLVDRRCAALLDEVVSLLD
CHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHC
ESPRIFLLDDEEGEVQQVSQDSDETAPTREVSAGRDLTRLPSDPLVAETVMPRATCDVAY
CCCCEEEEECCCCHHHHHCCCCCCCCCCHHHHCCCCHHCCCCCCCHHHHHCCCCCCCEEE
ILFTSGSTGAPKGVPISHGNACAYVAGQLHLIDRLPGARYIQLCELSFDPSVHDMFVCWA
EEEECCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCCCEEEEEEECCCCCCCEEEEEEE
NGACLYVPKTVEPIYNAAFIKEHAITHWSSVPSGAAFMQQFRKLQPDEFPSLRVTLFGGE
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
PLPRLLVQAWLRAAPNSRVLNMYGPTETTVACTAFEVTTEFLADPRRAVMPLGRALPGME
CHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHCCCCCE
LLIVDAALEPVAPGISGELLIGGPQVASGYLSADEPGNRRFISRMYPGHSASRWYRSADA
EEEEECHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
AREVAGEGIVFQGRLDTQIKIRGNRIELEEVEHVVRSCSQAALCAVIAWPVDEAGRAGGL
HHHHCCCCEEEECCCCCEEEEECCEECHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCE
IAFVTQVQGGATQILQACRQRLPAYAVPQRVVMLDTLPLNVNGKVDRRALAGQCMTGDVF
EEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHCCCCCCCCCC
T
C
>Mature Secondary Structure
MLDSFFFETAAREPDRPALWVDERSYCYDELASRAGRIAADVSAVLPAGRARRCLLFAHR
CCCHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECC
SVDAYAGLLGILNADCAYVPLNPNMPAARIAAIIEQSDAPVMLVDRRCAALLDEVVSLLD
CHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHC
ESPRIFLLDDEEGEVQQVSQDSDETAPTREVSAGRDLTRLPSDPLVAETVMPRATCDVAY
CCCCEEEEECCCCHHHHHCCCCCCCCCCHHHHCCCCHHCCCCCCCHHHHHCCCCCCCEEE
ILFTSGSTGAPKGVPISHGNACAYVAGQLHLIDRLPGARYIQLCELSFDPSVHDMFVCWA
EEEECCCCCCCCCCCCCCCCEEEEEECEEEEECCCCCCCEEEEEEECCCCCCCEEEEEEE
NGACLYVPKTVEPIYNAAFIKEHAITHWSSVPSGAAFMQQFRKLQPDEFPSLRVTLFGGE
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCC
PLPRLLVQAWLRAAPNSRVLNMYGPTETTVACTAFEVTTEFLADPRRAVMPLGRALPGME
CHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCHHHHHHHHHCCCCCE
LLIVDAALEPVAPGISGELLIGGPQVASGYLSADEPGNRRFISRMYPGHSASRWYRSADA
EEEEECHHHCCCCCCCCEEEECCHHHCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHH
AREVAGEGIVFQGRLDTQIKIRGNRIELEEVEHVVRSCSQAALCAVIAWPVDEAGRAGGL
HHHHCCCCEEEECCCCCEEEEECCEECHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCE
IAFVTQVQGGATQILQACRQRLPAYAVPQRVVMLDTLPLNVNGKVDRRALAGQCMTGDVF
EEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHCCCCCCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9352938; 10801488 [H]