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The map label for this gene is dnaK [H]

Identifier: 91783690

GI number: 91783690

Start: 2575642

End: 2577510

Strand: Direct

Name: dnaK [H]

Synonym: Bxe_A2124

Alternate gene names: 91783690

Gene position: 2575642-2577510 (Clockwise)

Preceding gene: 91783689

Following gene: 91783691

Centisome position: 52.61

GC content: 68.06

Gene sequence:

>1869_bases
ATGAGCGACGCACGCCAGCCAGGCACATCACGCTATGCCATCGGCATCGACCTCGGCACCACGCACTGCGCGCTGTCCTA
TGTGGACACGAGCGCGAGCGACGGCGAAAAGACCGCCCAGGGCGTGCTGCCAATCGCCCAGCTCACCGGTCCGGGCGCGA
TCGACAGCCTCGATCTGCTGCCCTCGTTTCTGTATCTGCCGCATCCCGACGAACTTGCGTCGGGCGACCTCTATCTGCCG
TGGACCGGCCAGCGCGAATTCGCGGTCGGCGAGTTCGCCCGCAGCCGCGGCGCGGCCACGCCGATCCGGCTCGTGTCGAG
CGCGAAGAGCTGGCTGTGCCATCCGGGAGTCGACCGGCGCGCCGCGATTCTGCCCAACGACGCGCCGCCCGAAGTCGCGC
GCGTCTCGCCGCTCGAAAGCTCGGTGCGTTATCTGACCCACTTGCGTGAAGCCTGGGATCATGCGCATCCCGACGCGCCG
TTCAGCGAACAGGACATCACGGTGACGATTCCGGCATCGTTCGATCCGGCCGCGCGTGAACTGACCGCCGAAGCTGCCGC
GGCCGCCGGCTACGGCAGCATGACGCTGCTGGAAGAACCGCAGGCCGCGCTGTATAGCTGGATCCAGAAAAGCGGCGGGC
AGTGGCGCAAGCAGGTCAAGGTCGGCGACATCATTCTCGTGGTGGACGTGGGCGGCGGCACGACCGACCTCTCGCTGATC
GCCGTGATCGAACGCGAAGGCAATCTCGAACTGCATCGCGTCGCGGTCGGCGAGCATATTCTGCTCGGCGGCGACAACAT
GGACCTCGCGCTCGCGCACGTGGTCGCGCGCAAGCTCGCGGGGCAAGGCACCCAGGCCGACGCATGGCAACTGCGCGCCC
TCACCTATGCGTGCCGCTCGGCCAAGGAAACGCTGCTCAGCGATCCGGCCACCGATACGGTGCCGCTCGTCGTGCCGAGC
CGCGGCTCGAAGCTGATCGGCGGCTCGATCCGCACCGAGCTCACGCGCGCCGAATTGACCCAGACGATTCTGGAAGGCTT
CTTCCCGCAGGTGGAGAGCGCCGCGCGTCCGGTGAGCCGCGCGCGCGCCGGTCTGACGCAACTCGGCCTGCCGTATGCGC
AGGACGCGGGCATCACGCGGCATCTGGCGGCGTTTCTCGGCCGTCAGGTGGGCGCGCTCGCCGAGCTCGAAGGACTGCGC
GATCTCGCCACGGCGCAAAACGCCAGCTTCCTGCATCCCACTGCCGTGCTGTTCAACGGCGGCGTGTTCAAGTCGCCGTT
GCTGGTCGAACGCATCATGGGCACGCTCAACGGCTGGCTCACAGCAGAAGGCGCGGCGCCGGCGCGGCTCCTCGAAGGCG
CCGATCTGGATCTCGCCGTCGCGCGCGGCGCAGCTTACTACGGTTACGTGCGACGCGGCCAGGGTGTGCGGATTCGCGGC
GGCACGGCACGCGCGTACTACATCGCGATCGAATCGGCGATGCCCGCGGTGCCCGGCCTCGAACCGCCCATCCAGGCGCT
GTGCGTGGCACCGTTCGGCATGGAAGAAGGCACCGAAGCCGAACTGCCCGCGCAGGAATTCGGGCTGGTGGTCGGCGAGC
CCGTGCATTTCCGCTTCTTCGGCTCGTCGGTGCGGCGGCAGGACCAGGTCGGCACGCTGCTCGACTTCTGGGGTCCTGAT
GAATTGCAGGAACTGGAAGAGATTCAGGCGACGCTGCCCGCCGCCGGCCGCAATGCCGGTGAAGTCGTGCCGGTGAAGCT
GCATGCGCGCGTCACCGAAGCGGGCACGCTCGAACTCGAAGCCGTGCCGCGTGGCACCGACGAGCGCTGGAAAGTCGAGT
TCGACGTGCGCGGCAACGCAAATGCATGA

Upstream 100 bases:

>100_bases
GCAGCATCAGCCATCGCGGCTGGCAGGTCGTCGAAGTGCGCCTGCCGAAGCTCGCGGACAGTCACAACGCGAAAGTGATC
GCACCGGCTGAGGTGGAGCT

Downstream 100 bases:

>100_bases
ATGAGATGAAGCGGTACAGCGTCGGCATCGATCTCGGCACCAGCAACACGGTGCTCGCCTATGCGCAGATGGGCGCACCA
GATGGGTCGCGGGTCGAGTC

Product: putative molecular chaperone protein HspA/DnaK

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MSDARQPGTSRYAIGIDLGTTHCALSYVDTSASDGEKTAQGVLPIAQLTGPGAIDSLDLLPSFLYLPHPDELASGDLYLP
WTGQREFAVGEFARSRGAATPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVARVSPLESSVRYLTHLREAWDHAHPDAP
FSEQDITVTIPASFDPAARELTAEAAAAAGYGSMTLLEEPQAALYSWIQKSGGQWRKQVKVGDIILVVDVGGGTTDLSLI
AVIEREGNLELHRVAVGEHILLGGDNMDLALAHVVARKLAGQGTQADAWQLRALTYACRSAKETLLSDPATDTVPLVVPS
RGSKLIGGSIRTELTRAELTQTILEGFFPQVESAARPVSRARAGLTQLGLPYAQDAGITRHLAAFLGRQVGALAELEGLR
DLATAQNASFLHPTAVLFNGGVFKSPLLVERIMGTLNGWLTAEGAAPARLLEGADLDLAVARGAAYYGYVRRGQGVRIRG
GTARAYYIAIESAMPAVPGLEPPIQALCVAPFGMEEGTEAELPAQEFGLVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPD
ELQELEEIQATLPAAGRNAGEVVPVKLHARVTEAGTLELEAVPRGTDERWKVEFDVRGNANA

Sequences:

>Translated_622_residues
MSDARQPGTSRYAIGIDLGTTHCALSYVDTSASDGEKTAQGVLPIAQLTGPGAIDSLDLLPSFLYLPHPDELASGDLYLP
WTGQREFAVGEFARSRGAATPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVARVSPLESSVRYLTHLREAWDHAHPDAP
FSEQDITVTIPASFDPAARELTAEAAAAAGYGSMTLLEEPQAALYSWIQKSGGQWRKQVKVGDIILVVDVGGGTTDLSLI
AVIEREGNLELHRVAVGEHILLGGDNMDLALAHVVARKLAGQGTQADAWQLRALTYACRSAKETLLSDPATDTVPLVVPS
RGSKLIGGSIRTELTRAELTQTILEGFFPQVESAARPVSRARAGLTQLGLPYAQDAGITRHLAAFLGRQVGALAELEGLR
DLATAQNASFLHPTAVLFNGGVFKSPLLVERIMGTLNGWLTAEGAAPARLLEGADLDLAVARGAAYYGYVRRGQGVRIRG
GTARAYYIAIESAMPAVPGLEPPIQALCVAPFGMEEGTEAELPAQEFGLVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPD
ELQELEEIQATLPAAGRNAGEVVPVKLHARVTEAGTLELEAVPRGTDERWKVEFDVRGNANA
>Mature_621_residues
SDARQPGTSRYAIGIDLGTTHCALSYVDTSASDGEKTAQGVLPIAQLTGPGAIDSLDLLPSFLYLPHPDELASGDLYLPW
TGQREFAVGEFARSRGAATPIRLVSSAKSWLCHPGVDRRAAILPNDAPPEVARVSPLESSVRYLTHLREAWDHAHPDAPF
SEQDITVTIPASFDPAARELTAEAAAAAGYGSMTLLEEPQAALYSWIQKSGGQWRKQVKVGDIILVVDVGGGTTDLSLIA
VIEREGNLELHRVAVGEHILLGGDNMDLALAHVVARKLAGQGTQADAWQLRALTYACRSAKETLLSDPATDTVPLVVPSR
GSKLIGGSIRTELTRAELTQTILEGFFPQVESAARPVSRARAGLTQLGLPYAQDAGITRHLAAFLGRQVGALAELEGLRD
LATAQNASFLHPTAVLFNGGVFKSPLLVERIMGTLNGWLTAEGAAPARLLEGADLDLAVARGAAYYGYVRRGQGVRIRGG
TARAYYIAIESAMPAVPGLEPPIQALCVAPFGMEEGTEAELPAQEFGLVVGEPVHFRFFGSSVRRQDQVGTLLDFWGPDE
LQELEEIQATLPAAGRNAGEVVPVKLHARVTEAGTLELEAVPRGTDERWKVEFDVRGNANA

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI124256496, Length=242, Percent_Identity=28.9256198347107, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788875, Length=317, Percent_Identity=28.7066246056782, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1786196, Length=413, Percent_Identity=24.2130750605327, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17534015, Length=392, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17534013, Length=392, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507981, Length=204, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320950, Length=376, Percent_Identity=24.468085106383, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323004, Length=383, Percent_Identity=23.4986945169713, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319396, Length=376, Percent_Identity=23.936170212766, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319314, Length=383, Percent_Identity=24.5430809399478, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324120, Length=352, Percent_Identity=26.4204545454545, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319972, Length=352, Percent_Identity=26.4204545454545, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737941, Length=322, Percent_Identity=25.776397515528, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17647515, Length=179, Percent_Identity=33.5195530726257, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24663992, Length=179, Percent_Identity=33.5195530726257, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24663988, Length=179, Percent_Identity=33.5195530726257, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 66367; Mature: 66236

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDARQPGTSRYAIGIDLGTTHCALSYVDTSASDGEKTAQGVLPIAQLTGPGAIDSLDLL
CCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHCCCCHHHHCCCCCCCHHHHH
PSFLYLPHPDELASGDLYLPWTGQREFAVGEFARSRGAATPIRLVSSAKSWLCHPGVDRR
HHHHCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
AAILPNDAPPEVARVSPLESSVRYLTHLREAWDHAHPDAPFSEQDITVTIPASFDPAARE
EEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHH
LTAEAAAAAGYGSMTLLEEPQAALYSWIQKSGGQWRKQVKVGDIILVVDVGGGTTDLSLI
HHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCHHHHEEEECCEEEEEECCCCCCCEEEE
AVIEREGNLELHRVAVGEHILLGGDNMDLALAHVVARKLAGQGTQADAWQLRALTYACRS
EEEECCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKETLLSDPATDTVPLVVPSRGSKLIGGSIRTELTRAELTQTILEGFFPQVESAARPVSR
HHHHHHCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAGLTQLGLPYAQDAGITRHLAAFLGRQVGALAELEGLRDLATAQNASFLHPTAVLFNG
HHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEECC
GVFKSPLLVERIMGTLNGWLTAEGAAPARLLEGADLDLAVARGAAYYGYVRRGQGVRIRG
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCEEEECCCCHHHHHEECCCCEEEEC
GTARAYYIAIESAMPAVPGLEPPIQALCVAPFGMEEGTEAELPAQEFGLVVGEPVHFRFF
CCCEEEEEEEECCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCHHHCCEEECCCEEEEEC
GSSVRRQDQVGTLLDFWGPDELQELEEIQATLPAAGRNAGEVVPVKLHARVTEAGTLELE
CCHHHHHHHHCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEE
AVPRGTDERWKVEFDVRGNANA
ECCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
SDARQPGTSRYAIGIDLGTTHCALSYVDTSASDGEKTAQGVLPIAQLTGPGAIDSLDLL
CCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHCCCCHHHHCCCCCCCHHHHH
PSFLYLPHPDELASGDLYLPWTGQREFAVGEFARSRGAATPIRLVSSAKSWLCHPGVDRR
HHHHCCCCCHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
AAILPNDAPPEVARVSPLESSVRYLTHLREAWDHAHPDAPFSEQDITVTIPASFDPAARE
EEECCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCHHHHH
LTAEAAAAAGYGSMTLLEEPQAALYSWIQKSGGQWRKQVKVGDIILVVDVGGGTTDLSLI
HHHHHHHHCCCCCEEEECCHHHHHHHHHHHCCCHHHHEEEECCEEEEEECCCCCCCEEEE
AVIEREGNLELHRVAVGEHILLGGDNMDLALAHVVARKLAGQGTQADAWQLRALTYACRS
EEEECCCCEEEEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKETLLSDPATDTVPLVVPSRGSKLIGGSIRTELTRAELTQTILEGFFPQVESAARPVSR
HHHHHHCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAGLTQLGLPYAQDAGITRHLAAFLGRQVGALAELEGLRDLATAQNASFLHPTAVLFNG
HHHCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEECC
GVFKSPLLVERIMGTLNGWLTAEGAAPARLLEGADLDLAVARGAAYYGYVRRGQGVRIRG
CCCCCHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCEEEECCCCHHHHHEECCCCEEEEC
GTARAYYIAIESAMPAVPGLEPPIQALCVAPFGMEEGTEAELPAQEFGLVVGEPVHFRFF
CCCEEEEEEEECCCCCCCCCCCCHHHHEECCCCCCCCCCCCCCHHHCCEEECCCEEEEEC
GSSVRRQDQVGTLLDFWGPDELQELEEIQATLPAAGRNAGEVVPVKLHARVTEAGTLELE
CCHHHHHHHHCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCEEEEE
AVPRGTDERWKVEFDVRGNANA
ECCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]