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The map label for this gene is nasA [H]

Identifier: 91783604

GI number: 91783604

Start: 2477440

End: 2478807

Strand: Direct

Name: nasA [H]

Synonym: Bxe_A2211

Alternate gene names: 91783604

Gene position: 2477440-2478807 (Clockwise)

Preceding gene: 91783603

Following gene: 91783605

Centisome position: 50.6

GC content: 64.47

Gene sequence:

>1368_bases
ATGAAAAACGTGATGAGCTCCCTAAAGAGCGGGGACTGGCGCGCGCTGGTGGCGTGTTTCCTCTATTTCGACACCGGCTT
TACCGTCTGGGTCCTGTATGGACCGCTCGCGCCGTTCATCAGCAAGAGCATTGCAATGACGCCCGCGCAACAAGGTTTGC
TGGTGGCGGTGCCGGTGCTCTCGGCCGCGATACTGCGCGTCACGCTCGGTAATCTTTATCAGTCCGCGCACGGCAAGCGC
ATTGCGTTGATGGGCGTGCTGCTTTCGGCAGTGCCGACCATCGTGCTGCCGTTGATGCCGTCTGTGCCGTCGTACACCGT
GCTGCTGGTGCTCGGCGTATTCCTCGGTGTCGGCGGTGCGAGTTTCGCCGTGGCGCTGCCGATGGCCGGCAGCAACTATC
CGCCGAAGGTGCAGGGTCTGGTGTTGGGCCTCGCCGCGGCCGGCAATATCGGCGCGGTGCTCGACGGCTTCCTGTTCCCG
CAACTGGCCACGCATTACGGCTGGCAGATGGCCGCCGGCGGCGCACTGCCGCTGCTCGCGATCGCGGCGATCACGCTGTA
TTTCTGGGCCAACGATTCCGGCGTGAAATCGGGCAGCGTGCTGCGCGCATTCGGCAGCTTTACGGTGACGCTGGTCGGAT
TGATCGTGCTGGTGCTGCTCGTCGAAGCGGGCATGTTCGGCTCCGGCAAGACCGGTGTGCTGCTGTTGCCCGTGATCGGC
GCCTTGCTGGCTATCGCGGTGCTGCCCAAGCGCTATCGCGCGGTGCTGGCCGAACGTGACACGTGGGCGATCATGCTGGT
GTACAGCATTACGTTCGGTGGTTTTGTCGGCATGTCCTCGTACGTTTCGCTGCTTCTCACCAACCTCTATCAGCTCTCGA
AGATCGACGCGGGTCTCTTCATGGCGCTGCTCGCCGCAACGGGCGCGATGGTGCGTCCGCTCGGCGGCCTGATCGCCGAC
AAGGTGTCCGGTGTGCGCGCGCTGACTTTCCTGCTCGCGATCATCTCGTTGTGCGACTTCGTGTTCGCCGCAGCCATGCC
TGCAATGGCGGGCGGCATTGCGCTGCTGTTGGTCCTGTATGTGGCCTTCGGTCTCGGCAACGGCGCGACCTTCCAGTTGG
TGCCGCATCGCTGGGCCGGCCGCACGGGGCTGATGTCGGGCATTGTCGGCGCCGCGGGCGGCATCGGCGGATTCTATCTG
CCGGTCGTGATGGGTATCGCGAAGGAAAGCACGGGCAGCTATCAGATGGGCTTTGCGACGTTCGGCGTGCTGGCCGCATG
CGCGTTCCTCGCGATCGTCGCGCTGCGCCGTCCGTGGATGGCGTGGTCGATGCACTCCGGCGTGATGCATGCGCACGCAA
CGGAGTAG

Upstream 100 bases:

>100_bases
TGCATGTTCATACCGGTTTTCAACATCGTGATCATTCCGGTGCGTAGCGCATGGCGCGATCTGCCGGATAGTCGAAAAAT
CAGCATTGTGAGGATATCTG

Downstream 100 bases:

>100_bases
TGGTGTGGCGTGGAGCGGAGTAGCGCTCCCGATCCGGCCGCCGCATTGACGCGGAAGCCGGGTCACACGCAACGGAGCCG
ATCAACCATCACCAGAAGTT

Product: major facilitator superfamily nitrate/nitrite transporter NarK/NasA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKNVMSSLKSGDWRALVACFLYFDTGFTVWVLYGPLAPFISKSIAMTPAQQGLLVAVPVLSAAILRVTLGNLYQSAHGKR
IALMGVLLSAVPTIVLPLMPSVPSYTVLLVLGVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFLFP
QLATHYGWQMAAGGALPLLAIAAITLYFWANDSGVKSGSVLRAFGSFTVTLVGLIVLVLLVEAGMFGSGKTGVLLLPVIG
ALLAIAVLPKRYRAVLAERDTWAIMLVYSITFGGFVGMSSYVSLLLTNLYQLSKIDAGLFMALLAATGAMVRPLGGLIAD
KVSGVRALTFLLAIISLCDFVFAAAMPAMAGGIALLLVLYVAFGLGNGATFQLVPHRWAGRTGLMSGIVGAAGGIGGFYL
PVVMGIAKESTGSYQMGFATFGVLAACAFLAIVALRRPWMAWSMHSGVMHAHATE

Sequences:

>Translated_455_residues
MKNVMSSLKSGDWRALVACFLYFDTGFTVWVLYGPLAPFISKSIAMTPAQQGLLVAVPVLSAAILRVTLGNLYQSAHGKR
IALMGVLLSAVPTIVLPLMPSVPSYTVLLVLGVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFLFP
QLATHYGWQMAAGGALPLLAIAAITLYFWANDSGVKSGSVLRAFGSFTVTLVGLIVLVLLVEAGMFGSGKTGVLLLPVIG
ALLAIAVLPKRYRAVLAERDTWAIMLVYSITFGGFVGMSSYVSLLLTNLYQLSKIDAGLFMALLAATGAMVRPLGGLIAD
KVSGVRALTFLLAIISLCDFVFAAAMPAMAGGIALLLVLYVAFGLGNGATFQLVPHRWAGRTGLMSGIVGAAGGIGGFYL
PVVMGIAKESTGSYQMGFATFGVLAACAFLAIVALRRPWMAWSMHSGVMHAHATE
>Mature_455_residues
MKNVMSSLKSGDWRALVACFLYFDTGFTVWVLYGPLAPFISKSIAMTPAQQGLLVAVPVLSAAILRVTLGNLYQSAHGKR
IALMGVLLSAVPTIVLPLMPSVPSYTVLLVLGVFLGVGGASFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFLFP
QLATHYGWQMAAGGALPLLAIAAITLYFWANDSGVKSGSVLRAFGSFTVTLVGLIVLVLLVEAGMFGSGKTGVLLLPVIG
ALLAIAVLPKRYRAVLAERDTWAIMLVYSITFGGFVGMSSYVSLLLTNLYQLSKIDAGLFMALLAATGAMVRPLGGLIAD
KVSGVRALTFLLAIISLCDFVFAAAMPAMAGGIALLLVLYVAFGLGNGATFQLVPHRWAGRTGLMSGIVGAAGGIGGFYL
PVVMGIAKESTGSYQMGFATFGVLAACAFLAIVALRRPWMAWSMHSGVMHAHATE

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47258; Mature: 47258

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVMSSLKSGDWRALVACFLYFDTGFTVWVLYGPLAPFISKSIAMTPAQQGLLVAVPVL
CCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEHHHH
SAAILRVTLGNLYQSAHGKRIALMGVLLSAVPTIVLPLMPSVPSYTVLLVLGVFLGVGGA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
SFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFLFPQLATHYGWQMAAGGALPLLA
HHEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHH
IAAITLYFWANDSGVKSGSVLRAFGSFTVTLVGLIVLVLLVEAGMFGSGKTGVLLLPVIG
HHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHH
ALLAIAVLPKRYRAVLAERDTWAIMLVYSITFGGFVGMSSYVSLLLTNLYQLSKIDAGLF
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MALLAATGAMVRPLGGLIADKVSGVRALTFLLAIISLCDFVFAAAMPAMAGGIALLLVLY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFGLGNGATFQLVPHRWAGRTGLMSGIVGAAGGIGGFYLPVVMGIAKESTGSYQMGFAT
HHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHHHH
FGVLAACAFLAIVALRRPWMAWSMHSGVMHAHATE
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKNVMSSLKSGDWRALVACFLYFDTGFTVWVLYGPLAPFISKSIAMTPAQQGLLVAVPVL
CCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEHHHH
SAAILRVTLGNLYQSAHGKRIALMGVLLSAVPTIVLPLMPSVPSYTVLLVLGVFLGVGGA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
SFAVALPMAGSNYPPKVQGLVLGLAAAGNIGAVLDGFLFPQLATHYGWQMAAGGALPLLA
HHEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHH
IAAITLYFWANDSGVKSGSVLRAFGSFTVTLVGLIVLVLLVEAGMFGSGKTGVLLLPVIG
HHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHH
ALLAIAVLPKRYRAVLAERDTWAIMLVYSITFGGFVGMSSYVSLLLTNLYQLSKIDAGLF
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MALLAATGAMVRPLGGLIADKVSGVRALTFLLAIISLCDFVFAAAMPAMAGGIALLLVLY
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFGLGNGATFQLVPHRWAGRTGLMSGIVGAAGGIGGFYLPVVMGIAKESTGSYQMGFAT
HHHCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEHHHHH
FGVLAACAFLAIVALRRPWMAWSMHSGVMHAHATE
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]