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The map label for this gene is insF1 [C]

Identifier: 91783373

GI number: 91783373

Start: 2219293

End: 2219973

Strand: Direct

Name: insF1 [C]

Synonym: Bxe_A2445

Alternate gene names: 91783373

Gene position: 2219293-2219973 (Clockwise)

Preceding gene: 91783372

Following gene: 91783374

Centisome position: 45.33

GC content: 61.97

Gene sequence:

>681_bases
GTGAAGTACGCGTTCATCGAGCGCAATCGACACCACTGGCCGGTCTCGGTGCTATGTGAAGTGCTGGAAGTCAGTCCCAG
CGGATATCATCAGCGCAGGCAACGCACCGCGCAGGCCAAGCCGCACCGAGGCCGCGTGAGTGACGACGCACTGCTTGCGC
ACATCAAGACGATTCACACGCAGGTCAGGGGCGAATACGGCTGGCCGCGCATGTGGAAGGAGCTGCTCACGCATGGCGTG
CGCGTGGGCAAGGAGCGGGTTCGCAAACTGATGGCACAGCATGGCATTCGAGCCCGGCACAAGCGCAAGTACATCGCGAC
GACAAACTCGAACCACGATTTGCCGGTTGCGCCAAATCTGCTGGAGCGCAATTTCACTGCAACTGCACCGAACCAGGTCT
GGACGACCGACATCACGTATCTGGCGACAGCCGAGGGCTGGGTGTACCTCGCGGTCATCATCGACCTGTTCAGCCGGCAG
GTGGTGGGCTGGTCGATGCAGCCGCACATGAAGGCTGAACTGGTCACGGACGCATTGCGCATGGCGTGGTTCCGACGCCG
CCCTGAAGCCGGCGTAATCGTGCATAGCGACAGGGGCAGCCTGCCGCGCGTCAAACAAGATGAGAGCAGCGCGTCAATCT
GGATGAGAGTACTGGCGTTGCGGCAGCGGTGGAGGGCGTAG

Upstream 100 bases:

>100_bases
AGCAGATGGAGCTGGCGCGGCTGCGTGCCGAAGTGGCTCGCCTGAAGATGGAGCGAGATATTTTAAAAAAAGCAACCGCG
TACTTCGCCAAGGAGTCGAT

Downstream 100 bases:

>100_bases
CCCGGAACCGCTGCCGCAACGCCGCGCCGAGGAGTCCGCCCCGCTGCCGGGGTGCCTTGTCGGTGGTCGCGGTAAATCGG
GTATGAACAGCTCTTCCATG

Product: putative IS3 type transposase integrase (orfB)

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKYAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHTQVRGEYGWPRMWKELLTHGV
RVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQ
VVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSLPRVKQDESSASIWMRVLALRQRWRA

Sequences:

>Translated_226_residues
MKYAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHTQVRGEYGWPRMWKELLTHGV
RVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQ
VVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSLPRVKQDESSASIWMRVLALRQRWRA
>Mature_226_residues
MKYAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHTQVRGEYGWPRMWKELLTHGV
RVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNLLERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQ
VVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHSDRGSLPRVKQDESSASIWMRVLALRQRWRA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=197, Percent_Identity=36.5482233502538, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1788405, Length=202, Percent_Identity=44.0594059405941, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787263, Length=202, Percent_Identity=44.0594059405941, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786753, Length=202, Percent_Identity=44.0594059405941, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786570, Length=202, Percent_Identity=44.0594059405941, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786490, Length=202, Percent_Identity=44.0594059405941, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789981, Length=194, Percent_Identity=28.3505154639175, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 26337; Mature: 26337

Theoretical pI: Translated: 11.44; Mature: 11.44

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHT
CCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QVRGEYGWPRMWKELLTHGVRVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCCCCCHH
LERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQVVGWSMQPHMKAELVTDALR
HHCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
MAWFRRRPEAGVIVHSDRGSLPRVKQDESSASIWMRVLALRQRWRA
HHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYAFIERNRHHWPVSVLCEVLEVSPSGYHQRRQRTAQAKPHRGRVSDDALLAHIKTIHT
CCCEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QVRGEYGWPRMWKELLTHGVRVGKERVRKLMAQHGIRARHKRKYIATTNSNHDLPVAPNL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCCCCCHH
LERNFTATAPNQVWTTDITYLATAEGWVYLAVIIDLFSRQVVGWSMQPHMKAELVTDALR
HHCCCCCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
MAWFRRRPEAGVIVHSDRGSLPRVKQDESSASIWMRVLALRQRWRA
HHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]